SEC23IP      CRISPR in K562      Batch: BGKcLV51

Experiment Information (Status: Sequencing)
BGKcLV51-95BGKcLV51-96
idx00
TRCN#_or_BGC#BGC#0000847BGC#0000847
shRNA_or_gRNA_sequenceAAGTAAGTTAGTGCCCGATGAAGTAAGTTAGTGCCCGATG
PAMAGGAGG
NameSEC23IP_94SEC23IP_94
Sample_IDBGKcLV51-95BGKcLV51-96
transduction_Date9/30/20259/30/2025
daysD6D6
RBP_nameSEC23IPSEC23IP
qPCR_result56.958.2
Ave_qPCR57.6
RT-qPCR_primer-Ftcctcagtgccagatgaatg
RT-qPCR_primer-Rccacaaacaccaaatggtca
protein_conc20272164
WB_result70.484.0
Ave_WB
WB_DONE_date10/30/25
MW
IP
antibody_Cat#A300-592Alot# 1
Antibody DCC IDENCAB911OUO
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID63366337




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
SEC23IPProduct_ID: A300-592A
Lot_ID: 1
Source: Bethyl Labs
Target Name: SEC23IP-human		 SEC23IP-HEPG2-CRISPR-A300-592A.png<br>Caption: Western blot following CRISPR against SEC23IP in HepG2 whole cell lysate using SEC23IP specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC23IP. SEC23IP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A300-592A_1_SEC23IP.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the SEC23IP specific antibody, A300-592A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the SEC23IP-specific antibody, A300-592A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 SEC23IP-K562-CRISPR-A300-592A.png<br>Caption: Western blot following CRISPR against SEC23IP in K562 whole cell lysate using SEC23IP specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against SEC23IP. SEC23IP protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV51-95BGKcLV51-96
Sample_IDBGKcLV51-95BGKcLV51-96
Sample Name
Sample NameSEC23IP-BGKcLV51-95SEC23IP-BGKcLV51-96
RBPSEC23IPSEC23IP
Cell_LineK562K562
Exp UID
StatusSequencingSequencing
Status_date2026-06-022026-06-02
ProjectENCORE2ENCORE2
Note
ID1458214583




Library-Prep Information
BGKcLV51-95BGKcLV51-96
Sample #1718
Sample NameSEC23IP-BGKcLV51-95SEC23IP-BGKcLV51-96
Sample_Name_Alias
Index Well PositionA03B03
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2026-05-182026-05-18
Lib_IDLib-260518Lib-260518
Tecan_Location
Tecan
Tecan_date
Size_bp292300
Peak_Molarity39.1041.10
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2026-05-212026-05-21
Sample_IDBGKcLV51-95BGKcLV51-96
RBPSEC23IPSEC23IP
Batch_IDBGKcLV51BGKcLV51
WB_result70.40084.000
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID48564857




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database