RIOK1

General Information

Gene Name       : RIOK1
Gene ID             : ENSG00000124784.8
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RIOK1)


Antibody Validation

 View validation images for all RIOK1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RIOK1ENSG00000124784.827.1424.0625.60ENCSR000AEF
HepG2RIOK1ENSG00000124784.821.7528.0624.91ENCSR985KAT
K562RIOK1ENSG00000124784.847.9249.1448.53ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RIOK1-BGKcLV30-K562ENCSR608WYZRIOK1K562ReleasedENCSR347NKX   CRISPRKD% = 56.7,58.9






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RIOK1ENCAB726SEPA302-456A1Released, ENCODE4BGHcLV34-33, Released, ENCODE4BGKcLV30-33, Released, ENCODE4
RIOK1ENCAB338ZHGA302-457A1Released, ENCODE4BGHcLV34-33, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RIOK1K562CRISPRENCSR608WYZENCSR347NKXRIOK1-BGKcLV30-33,RIOK1-BGKcLV30-34NT-BGKcLV30-1,NT-BGKcLV30-256.7,58.9ReleasedENCODE4
RIOK1HepG2CRISPRBGHcLV32-31,BGHcLV32-32BGHcLV32-1,BGHcLV32-20.0,0.0NotSatisfiedENCODE4
RIOK1HepG2CRISPRRIOK1-BGHcLV34-33B,RIOK1-BGHcLV34-34BNT-BGHcLV34-1B,NT-BGHcLV34-2B82.3,80.6SubmittingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RIOK1Product_ID: A302-456A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RIOK1-human		 RIOK1-HEPG2-CRISPR-A302-456A.png<br>Caption: Western blot following CRISPR against RIOK1 in HepG2 whole cell lysate using RIOK1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RIOK1. RIOK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A302-456A_1_RIOK1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RIOK1 specific antibody, A302-456A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RIOK1-specific antibody, A302-456A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RIOK1-K562-CRISPR-A302-456A.png<br>Caption: Western blot following CRISPR against RIOK1 in K562 whole cell lysate using RIOK1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RIOK1. RIOK1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
RIOK1Product_ID: A302-457A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RIOK1-human		 Bethyl_A302-457A_1_RIOK1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RIOK1 specific antibody, A302-457A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RIOK1-specific antibody, A302-457A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for RIOK1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RIOK1 RNA is bound by 14 proteins.  







eCLIP assay for RIOK1 is not avaliable on ENCORE database.





14 proteins are bound to RIOK1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr674178217417872+3'-UTR5.1696354.90861K562PUM2
chr674176957417751+3'-UTR3.850855.03335K562UCHL5
chr674030677403132+3'-UTR,CDS,intron-Proximal5.346598.41647HepG2BCLAF1
chr674012157401265+3'-UTR,CDS4.748654.90367K562ZNF622
chr674176897417737+3'-UTR4.4610714.69153K562PUM2
chr674012117401282+3'-UTR,CDS3.884005.07449K562DDX24
chr673900477390162+5'-UTR3.105655.50895HepG2NCBP2
chr673900567390127+5'-UTR4.585223.62829K562NCBP2
chr673901317390229+5'-UTR3.855089.36623K562DDX3X
chr674131227413175+CDS3.690926.84505K562ZNF622
chr674047857404800+CDS,intron-Proximal5.577078.88118K562AQR
chr674053377405491+CDS,intron-Distal,intron-Proximal3.663363.06150K562KHSRP
chr674144707414487+CDS,intron-Proximal3.785225.57819K562UCHL5
chr674051507405234+CDS,intron-Proximal3.318344.11795K562YBX3
chr674041717404204+CDS,intron-Proximal4.319363.38968HepG2BCLAF1
chr674116827411729+CDS,intron-Proximal5.8827912.46873K562AQR
chr674051737405224+CDS4.512265.91381K562LIN28B
chr674050927405166+CDS,intron-Proximal4.542724.23351K562BUD13
chr674131407413175+CDS3.234403.94979HepG2BCLAF1
chr673971337397198+intron-Distal4.775907.20766K562TARDBP
chr674117297411756+intron-Proximal4.292563.11721K562AQR
chr674048067404865+intron-Proximal4.384434.21433K562PRPF8
chr674048007404838+intron-Proximal5.8731111.02807K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RIOK1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RIOK1 is not available on ENCORE database.

  • 82 RBPs can affect the levels of RIOK1.  








The Knockdown/knockout RNA-Seq assay for RIOK1 is not available on ENCORE database.





82 RBPs can affect the gene expression of RIOK1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RIOK1ENSG00000124784.41040.69780625.090500.735306.315805.54900HepG2AKAP1
RIOK1ENSG00000124784.4445.92410813.20950-0.8668012.5090011.23600HepG2AUH
RIOK1ENSG00000124784.4315.38750431.05870-0.450802.165801.36800HepG2BCLAF1
RIOK1ENSG00000124784.4580.29740461.512500.330302.654101.99920HepG2CKAP4
RIOK1ENSG00000124784.4531.31220836.17430-0.654206.215205.21590HepG2CPSF6
RIOK1ENSG00000124784.4539.85600949.53630-0.814604.919403.63070HepG2DDX59
RIOK1ENSG00000124784.4245.11350372.39070-0.603404.518603.63690HepG2DNAJC21
RIOK1ENSG00000124784.4312.10990494.47960-0.663904.536903.78780HepG2EIF2S1
RIOK1ENSG00000124784.4489.19430358.848000.446902.932501.82400HepG2EIF3G
RIOK1ENSG00000124784.4884.81500679.706200.380403.868702.90830HepG2EIF4G1
RIOK1ENSG00000124784.4418.95250947.87700-1.1779015.1117013.73410HepG2GPKOW
RIOK1ENSG00000124784.4582.53750403.204300.530702.656501.56790HepG2GRSF1
RIOK1ENSG00000124784.4533.99930694.53920-0.379203.790202.96340HepG2HNRNPF
RIOK1ENSG00000124784.4512.17140904.57570-0.820608.863207.80690HepG2HNRNPLL
RIOK1ENSG00000124784.4339.79080424.32720-0.320502.180401.52060HepG2ILF2
RIOK1ENSG00000124784.4712.96480551.543700.370302.033101.46690HepG2MAGOH
RIOK1ENSG00000124784.4694.33390550.995000.333502.230901.50080HepG2MSI2
RIOK1ENSG00000124784.4586.79460922.53150-0.652704.216503.00580HepG2MTPAP
RIOK1ENSG00000124784.4534.49760802.07330-0.585604.998404.00380HepG2NSUN2
RIOK1ENSG00000124784.4762.24380513.774600.569007.104806.20650HepG2PCBP1
RIOK1ENSG00000124784.4379.79570652.12760-0.779908.081307.13430HepG2PUF60
RIOK1ENSG00000124784.4957.33400692.277800.467607.061306.20310HepG2PUM1
RIOK1ENSG00000124784.4650.01320450.369900.529302.191201.55760HepG2RAVER1
RIOK1ENSG00000124784.4533.04760358.762300.571103.064901.94830HepG2RCC2
RIOK1ENSG00000124784.4551.61190748.32550-0.440003.635402.87060HepG2RPLP0
RIOK1ENSG00000124784.4544.30340407.449400.417702.229201.50670HepG2RPS2
RIOK1ENSG00000124784.4503.21980372.174000.435102.020201.33880HepG2RRP9
RIOK1ENSG00000124784.4620.42950459.348400.433602.136301.30560HepG2SMNDC1
RIOK1ENSG00000124784.4647.33840996.23890-0.622003.621802.72360HepG2SNRNP70
RIOK1ENSG00000124784.4879.91570568.117300.631108.732607.99910HepG2SRSF1
RIOK1ENSG00000124784.4529.83320682.39340-0.365102.705902.01730HepG2SRSF7
RIOK1ENSG00000124784.4558.42200462.193900.272801.800801.33830HepG2SRSF9
RIOK1ENSG00000124784.4575.52340765.26950-0.411104.288703.67980HepG2SSB
RIOK1ENSG00000124784.4513.86230732.27530-0.511005.926705.07950HepG2SUCLG1
RIOK1ENSG00000124784.4332.94540485.56540-0.544402.923302.23080HepG2SUPV3L1
RIOK1ENSG00000124784.4668.01430881.65920-0.400302.819802.08930HepG2TARDBP
RIOK1ENSG00000124784.4863.90790582.305800.569004.808703.99390HepG2U2AF1
RIOK1ENSG00000124784.41014.49450558.245100.8617017.8029016.48180HepG2UTP3
RIOK1ENSG00000124784.42303.359602955.25790-0.359502.871501.62030K562ABCF1
RIOK1ENSG00000124784.41551.758302343.41590-0.594708.049207.13590K562AQR
RIOK1ENSG00000124784.41062.506401288.43450-0.278102.963101.97150K562BCLAF1
RIOK1ENSG00000124784.41166.653901808.01010-0.632006.613705.25140K562BOP1
RIOK1ENSG00000124784.4571.40940740.10750-0.373202.387201.42250K562CCAR1
RIOK1ENSG00000124784.41131.155201979.95600-0.807705.723604.00060K562CCDC124
RIOK1ENSG00000124784.42008.254602460.82440-0.293203.627302.35330K562CPEB4
RIOK1ENSG00000124784.41228.518601922.42780-0.6460010.291708.76470K562CPSF6
RIOK1ENSG00000124784.41384.916001129.708200.293702.897101.54320K562DDX27
RIOK1ENSG00000124784.43830.582102546.490500.5890016.8075015.32340K562DDX47
RIOK1ENSG00000124784.42593.355101855.225000.483105.521504.23080K562DKC1
RIOK1ENSG00000124784.4959.761101162.39810-0.276402.509701.37040K562EIF2S1
RIOK1ENSG00000124784.41136.719301881.47490-0.7270016.4306014.89290K562EIF2S2
RIOK1ENSG00000124784.41118.54630752.823200.571103.172002.08750K562EIF3A
RIOK1ENSG00000124784.41455.231401064.633400.450803.638502.73390K562EIF3G
RIOK1ENSG00000124784.41688.396102124.16800-0.331202.901502.03200K562FIP1L1
RIOK1ENSG00000124784.41187.345501956.03570-0.7202010.100608.41490K562FMR1
RIOK1ENSG00000124784.41128.497001657.92150-0.555005.479704.14120K562FTO
RIOK1ENSG00000124784.41643.523901149.742400.515408.674607.67540K562FXR1
RIOK1ENSG00000124784.41534.460901169.562200.391702.263001.63160K562G3BP2
RIOK1ENSG00000124784.41067.193601835.44090-0.7823012.7423011.02370K562GPKOW
RIOK1ENSG00000124784.41794.119301469.199300.288102.535501.50640K562HNRNPA1
RIOK1ENSG00000124784.4901.382601376.26890-0.610607.333406.40680K562HNRNPC
RIOK1ENSG00000124784.4990.288201228.85470-0.311402.339201.30420K562HNRNPF
RIOK1ENSG00000124784.41711.283301225.264200.481904.364302.77850K562HNRNPK
RIOK1ENSG00000124784.41371.477901627.02710-0.246502.280701.46840K562HNRNPU
RIOK1ENSG00000124784.41182.689601567.24960-0.406202.365401.51140K562KRR1
RIOK1ENSG00000124784.41703.782401289.215200.402104.236303.19200K562MAGOH
RIOK1ENSG00000124784.41300.169401791.59700-0.462505.192903.97060K562METAP2
RIOK1ENSG00000124784.42164.747503169.07770-0.549906.728404.99240K562MSI2
RIOK1ENSG00000124784.41642.567501991.57190-0.278004.809503.86340K562NSUN2
RIOK1ENSG00000124784.41137.499201635.69140-0.524004.682503.60150K562PCBP3
RIOK1ENSG00000124784.4965.191201254.57690-0.378303.632202.70850K562POLR2G
RIOK1ENSG00000124784.41573.400401085.234000.535805.555404.49170K562PRPF8
RIOK1ENSG00000124784.4957.068801233.92610-0.366602.647301.76680K562PSIP1
RIOK1ENSG00000124784.44000.930303449.855700.213702.422501.42860K562PUM1
RIOK1ENSG00000124784.42637.884103380.73400-0.358004.555703.31510K562PUM2
RIOK1ENSG00000124784.42212.887601709.879400.371902.931902.37580K562PUS1
RIOK1ENSG00000124784.42180.121801665.705300.388204.136403.42750K562RBM15
RIOK1ENSG00000124784.4897.832301125.58970-0.326202.426401.50260K562RCC2
RIOK1ENSG00000124784.41688.892001105.525800.611206.076004.86060K562RPS19
RIOK1ENSG00000124784.41467.927701118.028500.392703.707302.44860K562SAFB
RIOK1ENSG00000124784.41188.526401965.09810-0.7254016.9030015.02630K562SF3A3
RIOK1ENSG00000124784.41316.146901745.58610-0.407403.019102.00200K562SF3B1
RIOK1ENSG00000124784.41797.446301227.862000.549707.622906.45660K562SRSF1
RIOK1ENSG00000124784.41991.386701258.448200.6620011.7560010.06970K562SYNCRIP
RIOK1ENSG00000124784.4863.738901242.75190-0.524904.974304.01180K562TARDBP
RIOK1ENSG00000124784.4780.314801186.85570-0.605005.595104.22000K562TIAL1
RIOK1ENSG00000124784.4974.927701260.64870-0.370802.978301.98880K562TRIM56
RIOK1ENSG00000124784.41903.314502485.36120-0.384903.515101.91530K562TRIP6
RIOK1ENSG00000124784.41904.379902281.75550-0.260803.576802.12340K562TROVE2
RIOK1ENSG00000124784.41539.454501170.869900.394704.351503.35000K562U2AF2
RIOK1ENSG00000124784.4723.774801112.17260-0.619805.861004.32570K562UCHL5
RIOK1ENSG00000124784.41567.118102111.70190-0.430308.379407.21250K562UPF1
RIOK1ENSG00000124784.41446.809302118.15350-0.549905.199203.55140K562UTP3
RIOK1ENSG00000124784.41360.038101806.92320-0.409904.545403.41020K562WDR3
RIOK1ENSG00000124784.42139.752901878.945600.187402.102301.35700K562WDR43
RIOK1ENSG00000124784.41947.960602639.93870-0.438504.999103.78480K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RIOK1 is not available on ENCORE database.





18 RBPs can affect the splicing of RIOK1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERIOK1ENSG00000124784.4chr6+739893073989737396873739700574011907401283-0.123000.000000.00000excludedK562AQR
SERIOK1ENSG00000124784.4chr6+741312174131757411475741168474144707414623-0.085000.000000.00000excludedK562AQR
SERIOK1ENSG00000124784.4chr6+741312174131757411564741168474144707414623-0.059000.000170.00381excludedK562PUS1
SERIOK1ENSG00000124784.4chr6+7393112739320273897287390306739333173935360.097000.000000.00003includedK562U2AF1
SERIOK1ENSG00000124784.4chr6+7393112739320273897287390306739333173935360.085000.000010.01043includedK562TBRG4
SERIOK1ENSG00000124784.4chr6+739528573953767393331739353674011907401283-0.065000.002980.08598excludedK562HNRNPU
SERIOK1ENSG00000124784.4chr6+741312174131757411564741168474144707414623-0.060000.000140.00872excludedK562SF3B1
A3SSRIOK1ENSG00000124784.4chr6+739525373953767395285739537673933407393536-0.062000.000500.04661shorterK562AKAP8L
A3SSRIOK1ENSG00000124784.4chr6+7404635740478874046507404788740417374042600.230000.000000.00000longerK562AQR
A3SSRIOK1ENSG00000124784.4chr6+7395253739537673952857395376739333173935360.191000.000000.00001longerK562HNRNPC
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.055000.003130.04341includedK562SF3B4
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.110000.000020.00027includedHepG2U2AF2
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.093000.000390.02984includedHepG2HNRNPUL1
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.061000.001200.07195includedK562PHF6
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.084000.000030.00881includedK562UTP3
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.091000.003280.03565includedHepG2QKI
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.138000.000220.00352includedHepG2TAF15
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.059000.001300.01959includedK562U2AF2
RIRIOK1ENSG00000124784.4chr6+7402835740313074028357402948740304974031300.086000.005270.06760includedHepG2SRSF9
RIRIOK1ENSG00000124784.4chr6+740283574031307402835740294874030497403130-0.069000.000380.03411excludedK562FXR2
MXERIOK1ENSG00000124784.4chr6+739525373953767396935739700573933317393536739893073989730.051000.002260.066011includedK562LIN28B
MXERIOK1ENSG00000124784.4chr6+73969357397005739893073989737395285739537674011907401283-0.181000.001650.053962includedK562LIN28B
MXERIOK1ENSG00000124784.4chr6+739525373953767396935739700573933317393536739893073989730.128000.000000.000021includedK562HNRNPC
MXERIOK1ENSG00000124784.4chr6+73969357397005739893073989737395285739537674011907401283-0.157000.004270.062122includedK562HNRNPC

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RIOK1 is not avaliable on ENCORE database.