EED

General Information

Gene Name       : EED
Gene ID             : ENSG00000074266.19
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: EED)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878EEDENSG00000074266.1939.0134.7536.88ENCSR000AEF
HepG2EEDENSG00000074266.1913.7319.1616.45ENCSR985KAT
K562EEDENSG00000074266.1930.2429.0229.63ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
eCLIPeCLIP: 4223ENCSR171XCREEDK562ReleasedENCAB164MTMENCSR818IOS  






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
EED4223K562ENCSR171XCRENCSR818IOSENCAB164MTMPA5-34430UK2881462AReleasedENCODE4


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
EED85322S1Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
EEDK562CRISPRBGKcLV23-33,BGKcLV23-34BGKcLV23-1,BGKcLV23-2NO CLEAR BAND,NotSatisfiedENCODE4
EEDK562CRISPRBGKcLV23-35,BGKcLV23-36BGKcLV23-1,BGKcLV23-2NO CLEAR BAND,NotSatisfiedENCODE4
EEDK562CRISPRBGKcLV44-21,BGKcLV44-22BGKcLV44-1,BGKcLV44-223.7,29.3NotSatisfiedENCORE2
EEDK562CRISPRBGKcLV46-25,BGKcLV46-26BGKcLV46-1,BGKcLV46-253.8,68.6ExperimentedENCORE2
EEDK562CRISPRBGKcLV46-27,BGKcLV46-28BGKcLV46-1,BGKcLV46-256.8,59.5ExperimentedENCORE2
EEDHepG2CRISPRBGHcLV24-25,BGHcLV24-26BGHcLV24-1,BGHcLV24-2NotSatisfiedENCODE4
EEDHepG2CRISPRBGHcLV24-27,BGHcLV24-28BGHcLV24-1,BGHcLV24-2NotSatisfiedENCODE4
EEDHepG2CRISPRBGHcLV45-27,BGHcLV45-28BGHcLV45-1,BGHcLV45-279.5,63.0SequencingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EEDProduct_ID: 85322S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EED-human		 CST_85322S_1_EED.png<br>Caption: IP-WB analysis of 85322S whole cell lysate using the EED specific antibody, 85322S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EED-specific antibody, 85322S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for EED is not avaliable on ENCORE database.



Proteins bound to RNA
  • EED RNA is bound by 26 proteins.  







eCLIP assay for EED is not avaliable on ENCORE database.





26 proteins are bound to EED RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr118598960585989741+3'-UTR4.723489.27158K562CPEB4
chr118598957485989691+3'-UTR4.895765.64557HepG2KHSRP
chr118598958085989672+3'-UTR3.550068.33024K562PUM2
chr118598961785989669+3'-UTR4.0553815.38759K562PABPC4
chr118598958185989678+3'-UTR3.2585710.80134K562DDX6
chr118595601985956083+5'-UTR5.8169410.00399K562GTF2F1
chr118595600285956084+5'-UTR4.814995.84876HepG2GTF2F1
chr118595597585956085+5'-UTR4.8834812.13666HepG2PRPF4
chr118595615185956224+5'-UTR3.294413.94360HepG2GTF2F1
chr118595599985956094+5'-UTR3.017053.17504K562CSTF2T
chr118595581785955953+5'-UTR4.564424.61711HepG2NCBP2
chr118595598785956078+5'-UTR3.780155.64899HepG2PCBP2
chr118595583685955970+5'-UTR4.0759111.58765K562DDX3X
chr118595615585956227+5'-UTR4.488445.44815HepG2PRPF4
chr118595607785956141+5'-UTR4.789835.89791K562RBM15
chr118595600985956090+5'-UTR4.255786.11943K562UCHL5
chr118598801885988127+5'-UTR,CDS,intron-Proximal5.220397.04013HepG2BCLAF1
chr118595587685955958+5'-UTR3.548984.93572K562LIN28B
chr118595614685956261+5'-UTR3.162354.68262K562RBM15
chr118595601285956103+5'-UTR4.394477.35664HepG2BUD13
chr118595600385956107+5'-UTR4.805355.03746HepG2BCLAF1
chr118595598185956089+5'-UTR3.113093.54210HepG2EIF3H
chr118596148385961558+CDS,intron-Distal,intron-Proximal3.223463.78727K562PRPF8
chr118596755085967600+CDS,intron-Distal,intron-Proximal3.986075.26367K562AQR
chr118596860885968712+CDS,intron-Distal,intron-Proximal5.763314.02817HepG2PRPF8
chr118597712485977202+CDS,intron-Distal,intron-Proximal3.844364.76554HepG2BCLAF1
chr118596626385966303+CDS,intron-Distal,intron-Proximal4.187843.71570HepG2BCLAF1
chr118596753485967622+CDS,intron-Distal,intron-Proximal4.837874.96013K562PRPF8
chr118596324085963286+CDS,intron-Distal,intron-Proximal4.982177.04013HepG2BCLAF1
chr118596143285961503+CDS,intron-Distal,intron-Proximal3.085853.70975K562UCHL5
chr118596319585963274+CDS,intron-Distal5.350445.73530K562DDX24
chr118596860785968683+CDS,intron-Distal,intron-Proximal3.502677.75987K562PRPF8
chr118596318785963240+CDS,intron-Distal,intron-Proximal3.484023.28366HepG2BCLAF1
chr118596823785968310+intron-Distal5.2834010.50009K562TARDBP
chr118598347485983582+intron-Distal3.346053.72904K562EXOSC5
chr118598821785988315+intron-Distal,intron-Proximal4.131423.31283K562PRPF8
chr118596868385968718+intron-Distal,intron-Proximal3.953183.02181K562PRPF8
chr118598820485988364+intron-Distal,intron-Proximal3.6039010.59064K562KHSRP
chr118598595385986017+intron-Distal4.194094.27460K562HNRNPM
chr118597965285979727+intron-Distal,intron-Proximal3.021133.07368K562TARDBP
chr118598299285983033+intron-Distal4.007023.01456K562EXOSC5
chr118598606185986158+intron-Distal4.457215.83842HepG2SUGP2
chr118598254085982586+intron-Distal4.261094.48560K562EXOSC5
chr118598596485986040+intron-Distal4.426553.59917K562EXOSC5
chr118598872785988790+intron-Distal3.301914.10408K562KHSRP
chr118596874785968875+intron-Distal3.344446.65670K562HNRNPL
chr118598555185985638+intron-Distal3.359173.59509K562HNRNPM
chr118596875585968856+intron-Distal3.718315.63547HepG2HNRNPL
chr118596863885968699+intron-Distal,intron-Proximal4.892345.17615K562AQR
chr118598888385988928+intron-Proximal3.040333.54212HepG2RBM5
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for EED is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for EED is not available on ENCORE database.

  • 63 RBPs can affect the levels of EED.  








The Knockdown/knockout RNA-Seq assay for EED is not available on ENCORE database.





63 RBPs can affect the gene expression of EED :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
EEDENSG00000074266.13323.79900216.177800.582802.409401.52980HepG2ABCF1
EEDENSG00000074266.13143.73210246.20360-0.776502.372301.72880HepG2CPSF6
EEDENSG00000074266.13348.03910498.91600-0.519504.923903.97570HepG2DAZAP1
EEDENSG00000074266.13159.69700239.39920-0.584103.475902.75930HepG2DNAJC2
EEDENSG00000074266.13325.90730471.51520-0.532803.378702.48400HepG2EIF4G2
EEDENSG00000074266.13413.00140307.828800.423902.038001.44100HepG2FXR1
EEDENSG00000074266.13150.57010279.12020-0.890504.810203.98100HepG2GPKOW
EEDENSG00000074266.13210.94290287.28070-0.445602.544701.85600HepG2HNRNPF
EEDENSG00000074266.13167.25930266.36690-0.671302.225901.65490HepG2HNRNPLL
EEDENSG00000074266.13165.26170269.44510-0.705204.819604.03760HepG2HNRNPU
EEDENSG00000074266.13197.63500282.88800-0.517402.709501.99340HepG2IGF2BP1
EEDENSG00000074266.13185.91590275.22950-0.566003.999703.13470HepG2ILF2
EEDENSG00000074266.13433.98810307.936000.494902.599601.96320HepG2MAGOH
EEDENSG00000074266.13466.75260309.670600.591803.308802.62460HepG2MATR3
EEDENSG00000074266.13150.07460236.16390-0.654102.761502.01410HepG2NSUN2
EEDENSG00000074266.13150.33720240.32380-0.676803.892303.03330HepG2PPIL4
EEDENSG00000074266.13300.13450472.26150-0.654005.038304.26350HepG2PUF60
EEDENSG00000074266.13383.04760501.33040-0.388203.012402.39950HepG2PUM1
EEDENSG00000074266.13357.33560500.96170-0.487403.442802.69770HepG2PUM2
EEDENSG00000074266.13430.62110327.357200.395502.374201.86620HepG2NELFE
EEDENSG00000074266.13140.94170239.95390-0.767705.029604.20640HepG2SAFB2
EEDENSG00000074266.13400.22740257.036500.638703.611102.83740HepG2SF3A3
EEDENSG00000074266.13179.30420263.66750-0.556302.081201.46300HepG2SF3B1
EEDENSG00000074266.13283.69170452.83330-0.674706.007005.03510HepG2SLBP
EEDENSG00000074266.13196.63610293.37020-0.577202.071601.39970HepG2SNRNP70
EEDENSG00000074266.13422.09920562.18540-0.413503.298702.76680HepG2SRFBP1
EEDENSG00000074266.13738.90380319.653201.2088022.6678021.59550HepG2SRSF1
EEDENSG00000074266.13172.45830282.24880-0.710705.228504.31960HepG2SRSF7
EEDENSG00000074266.13102.42580184.25710-0.847103.350501.87900HepG2SUGP2
EEDENSG00000074266.13174.31330259.54770-0.574302.526001.82910HepG2TARDBP
EEDENSG00000074266.13156.61220261.82350-0.741404.752704.13360HepG2TIAL1
EEDENSG00000074266.13135.95680264.15620-0.958207.845006.97360HepG2TRIM56
EEDENSG00000074266.13150.79990304.88750-1.015602.500201.56760HepG2U2AF2
EEDENSG00000074266.13340.73520220.079000.630504.361503.63740HepG2UTP3
EEDENSG00000074266.13794.74160424.796200.9036011.306308.96180K562AARS
EEDENSG00000074266.131136.75650913.986000.314602.642202.08420K562AGGF1
EEDENSG00000074266.131363.43030951.046200.519605.655004.70250K562AKAP8L
EEDENSG00000074266.131227.867001637.23770-0.415103.731603.07050K562AQR
EEDENSG00000074266.13727.58330871.78180-0.260902.274801.40570K562BCLAF1
EEDENSG00000074266.13172.02650269.66830-0.648602.411201.44180K562CCAR1
EEDENSG00000074266.131557.733501092.255700.512006.985205.09940K562CCDC124
EEDENSG00000074266.131562.315702013.10760-0.365703.935402.76140K562CELF1
EEDENSG00000074266.13557.75070709.84910-0.347902.806501.58660K562DDX21
EEDENSG00000074266.131310.91610962.421400.445706.390205.45800K562DDX47
EEDENSG00000074266.131324.759801023.379100.372304.407703.26540K562DKC1
EEDENSG00000074266.13975.41030783.026500.316802.531101.76960K562EIF3G
EEDENSG00000074266.13528.88260695.90820-0.395902.638801.81040K562FIP1L1
EEDENSG00000074266.131051.92570813.255200.371202.664602.06880K562FXR1
EEDENSG00000074266.13546.85430687.50890-0.330202.432101.36870K562GLRX3
EEDENSG00000074266.13346.66730517.01280-0.576604.209203.23530K562GPKOW
EEDENSG00000074266.13761.84110961.97770-0.336502.676001.62160K562HNRNPA1
EEDENSG00000074266.13345.32400464.85170-0.428802.454001.88800K562HNRNPC
EEDENSG00000074266.13286.57750415.09580-0.534503.375902.10820K562HNRNPF
EEDENSG00000074266.13608.948501065.28870-0.8069014.1482012.18800K562HNRNPU
EEDENSG00000074266.131308.693601009.289500.374703.025602.15990K562KIF1C
EEDENSG00000074266.131254.11470966.145100.376302.903901.79580K562MAK16
EEDENSG00000074266.13982.574701203.16600-0.292202.296001.46220K562MATR3
EEDENSG00000074266.13500.02340722.83040-0.531704.264203.27850K562MTPAP
EEDENSG00000074266.131086.76760792.511700.455402.720301.84570K562NAA15
EEDENSG00000074266.13570.20570803.10430-0.494106.425805.35230K562NSUN2
EEDENSG00000074266.131367.537201682.37960-0.298902.836101.37800K562PNPT1
EEDENSG00000074266.13933.54200677.982700.461402.298601.61250K562PRPF8
EEDENSG00000074266.132252.123201900.078100.245102.381101.39720K562PUM1
EEDENSG00000074266.131657.91670943.160100.8137011.3923010.33770K562PUS1
EEDENSG00000074266.131030.53100690.801600.576903.579902.64850K562RPS19
EEDENSG00000074266.13641.47340792.49650-0.305002.500701.72240K562SF3A3
EEDENSG00000074266.13665.99120830.82870-0.319002.382601.59130K562SF3B4
EEDENSG00000074266.131605.122701148.247800.483105.732304.72180K562SRSF1
EEDENSG00000074266.13479.43190633.48090-0.402003.458902.14020K562SRSF7
EEDENSG00000074266.131241.061401558.69080-0.328803.046701.46490K562SUCLG1
EEDENSG00000074266.13749.10260539.987300.472103.198502.31490K562SYNCRIP
EEDENSG00000074266.13844.228501162.13350-0.461103.892103.04660K562TARDBP
EEDENSG00000074266.13227.62110400.87880-0.816505.656004.27340K562TIAL1
EEDENSG00000074266.13305.98870425.85590-0.476902.853601.89040K562TRIM56
EEDENSG00000074266.13685.33110851.55050-0.313302.814402.09000K562UPF1
EEDENSG00000074266.131313.946101844.42870-0.489305.585704.28750K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for EED is not available on ENCORE database.





24 RBPs can affect the splicing of EED :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.145000.000000.00000includedK562SRSF1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.063000.001690.03212includedK562SF3B4
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.077000.006370.08899excludedK562SF3B4
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.159000.001080.06351includedHepG2RBM22
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.054000.002800.06838excludedK562AKAP8L
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.456000.000040.00900includedHepG2HNRNPM
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.171000.000180.02125includedK562XRCC6
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.075000.000010.00800includedK562PUM1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.267000.000000.00055includedK562FMR1
SEEEDENSG00000074266.13chr11+8598112485981199859794978597960385988021859881800.506000.000000.00000includedHepG2U2AF2
SEEEDENSG00000074266.13chr11+859794978597960385975213859753058598802185988180-0.436000.000870.01251excludedHepG2U2AF2
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.101000.000000.00004excludedK562AQR
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.110000.000000.00001includedK562FXR1
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.079000.000190.00429excludedK562PUS1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.336000.000000.00011includedHepG2MATR3
SEEEDENSG00000074266.13chr11+859685568596863885967428859675548597521385975305-0.236000.000000.00002excludedK562U2AF1
SEEEDENSG00000074266.13chr11+8598112485981199859794978597960385988021859881800.250000.000010.00476includedK562HNRNPM
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.407000.000070.00860includedHepG2TIAL1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.084000.000000.00609includedK562PUM2
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.248000.000070.01162includedHepG2SUPV3L1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.084000.000030.00961includedK562RBM22
SEEEDENSG00000074266.13chr11+859613378596149085956309859563858598802185988577-0.059000.000060.00381excludedK562UTP18
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.072000.000290.00716includedK562U2AF2
SEEEDENSG00000074266.13chr11+859685568596863885967428859675548597521385975305-0.203000.000000.00009excludedK562U2AF2
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.092000.000740.01553excludedK562U2AF2
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.114000.000000.00005includedK562TARDBP
SEEEDENSG00000074266.13chr11+859685568596863885967428859675548597521385975305-0.129000.000350.01204excludedK562AGGF1
SEEEDENSG00000074266.13chr11+859794978597960385977124859772588598802185988180-0.075000.000320.01143excludedK562AGGF1
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.095000.000030.00847includedK562ZC3H8
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.072000.000040.02504includedK562NCBP2
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.227000.000020.02007includedHepG2YBX3
SEEEDENSG00000074266.13chr11+8598112485981199859789368597960385988021859881800.136000.002730.09097includedHepG2HNRNPU
A3SSEEDENSG00000074266.13chr11+8598881385989033859889598598903385988131859881800.135000.000300.06487longerK562SUPV3L1
MXEEEDENSG00000074266.13chr11+8596855685968638859752138597530585967428859675548598802185988180-0.192000.000000.000002includedK562AQR
MXEEEDENSG00000074266.13chr11+85988813859889168598895985989033859880218598818085989440859897780.052000.001160.045611includedK562HNRNPU

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for EED is not avaliable on ENCORE database.