EED antibody: 85322S


Antibody Info
Product_ID85322S
Lot_ID1
Host_organismRabbit
SourceCell Signaling Technology
Antibody_DCC_ID
Target Info
GeneNameEED
Phosphate
Target_nameEED-human

Validation Images
Cell linePrimary validationSecondary validation
HepG2 EED-HEPG2-CRISPR-85322S.png<br>Caption: Western blot following CRISPR against EED in HepG2 whole cell lysate using EED specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EED. EED protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Caption: Western blot following CRISPR against EED in HepG2 whole cell lysate using EED specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EED. EED protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.

Releated Sample: BGHcLV45-27

Status: Experimented

Lab: Graveley Lab

K562 CST_85322S_1_EED.png<br>Caption: IP-WB analysis of 85322S whole cell lysate using the EED specific antibody, 85322S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EED-specific antibody, 85322S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Caption: IP-WB analysis of 85322S whole cell lysate using the EED specific antibody, 85322S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EED-specific antibody, 85322S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.

Method: immunoprecipitation

Releated Sample: eCLIP:4223

Status: Submitted

Lab: Yeo Lab