DICER1

General Information

Gene Name       : DICER1
Gene ID             : ENSG00000100697.14
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: DICER1)


Antibody Validation

 View validation images for all DICER1 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DICER1ENSG00000100697.1427.2229.6728.45ENCSR000AEF
HepG2DICER1ENSG00000100697.1414.0426.1120.07ENCSR985KAT
K562DICER1ENSG00000100697.149.838.329.07ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DICER1-BGKcLV15-K562ENCSR715XCSDICER1K562ReleasedENCSR404OHQ   CRISPRKD% = 78.0,71.9
knockdown followed by RNA-seq DICER1-BGHcLV16-HepG2ENCSR428WVZDICER1HepG2ReleasedENCSR964HKT   CRISPRKD% = ,






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DICER1ENCAB330RCKPA5-17021TL2693039BGKcLV15-11, Released, ENCODE4
DICER1ENCAB582NBRRN030PW1Released, ENCODE3BGKcLV15-11, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DICER1K562shRNABGKLV29-23,BGKLV29-24BGKLV29-1,BGKLV29-278.5,71.1NotSatisfiedENCODE3
DICER1K562CRISPRENCSR715XCSENCSR404OHQDICER1-BGKcLV15-11,DICER1-BGKcLV15-12NT-BGKcLV15-1,NT-BGKcLV15-278.0,71.9ReleasedENCODE4
DICER1HepG2CRISPRENCSR428WVZENCSR964HKTDICER1-BGHcLV16-13,DICER1-BGHcLV16-14NT-BGHcLV16-1,NT-BGHcLV16-2,ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DICER1Product_ID: RN030PW
Lot_ID: 1
Source: MBLI
Target Name: DICER1-human		 DICER1_HepG2.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DICER1 specific antibody. Lane 1 is 3% of ten million whole cell lysate input (lane under '15% input') , lane 2 is 20% of IP enrichment using rabbit normal IgG (lane under 'IgG') and lane 3 is 20% IP enrichment using rabbit polyclonal anti-DICER1antibody (lanes under 'anti-DICER1'). Asterisk indicates heavy chain of antibody.
DICER1Product_ID: PA5-17021
Lot_ID: TL2693039
Source: Thermo Fisher
Target Name: DICER1-human		 DICER1-K562-CRISPR-PA5-17021.png<br>Caption: Western blot following CRISPR against DICER1 in K562 whole cell lysate using DICER1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DICER1. DICER1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DICER1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • DICER1 RNA is bound by 21 proteins.  







eCLIP assay for DICER1 is not avaliable on ENCORE database.





21 proteins are bound to DICER1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr149555689095556937-3'-UTR,CDS,intron-Distal,intron-Proximal3.676665.97823HepG2BCLAF1
chr149555425695554341-3'-UTR,intron-Distal3.702365.78005K562KHSRP
chr149555409295554242-3'-UTR,intron-Distal4.775906.02873K562TARDBP
chr149555426595554319-3'-UTR,intron-Distal4.980869.83252HepG2FUBP3
chr149555392595554010-3'-UTR,intron-Distal4.398516.54171HepG2DDX55
chr149562349795623595-5'-UTR,intron-Distal3.382107.35853HepG2PRPF8
chr149557143895571505-5'-UTR,CDS,intron-Distal4.376014.01763HepG2GRWD1
chr149557201795572106-5'-UTR,CDS,intron-Distal3.334809.64262HepG2BCLAF1
chr149557203295572104-5'-UTR,CDS,intron-Distal3.175933.79741HepG2GRWD1
chr149562359695623681-5'-UTR,intron-Distal,intron-Proximal3.148103.64037HepG2NCBP2
chr149557145495571495-5'-UTR,CDS,intron-Distal3.457785.91405HepG2BCLAF1
chr149562360295623677-5'-UTR,intron-Distal,intron-Proximal3.081509.70395HepG2DDX3X
chr149562372795623769-5'-UTR,intron-Distal,intron-Proximal4.663014.66990HepG2DDX3X
chr149557205295572106-5'-UTR,CDS,intron-Distal5.121376.71899HepG2FXR2
chr149556292295563031-CDS,intron-Distal6.6797423.22211HepG2FXR2
chr149558395895584084-CDS,intron-Distal,intron-Proximal4.807368.02070HepG2FXR2
chr149559076895590872-CDS,intron-Distal3.130446.42929HepG2GRWD1
chr149556994795570083-CDS,intron-Distal,intron-Proximal3.6107812.15188HepG2FXR2
chr149559797395598053-CDS,intron-Distal,intron-Proximal4.574994.97857HepG2AQR
chr149559892095598979-CDS,intron-Distal,intron-Proximal3.377105.63425HepG2BCLAF1
chr149558398695584041-CDS,intron-Distal3.8776310.36594HepG2BCLAF1
chr149559581595595853-CDS,intron-Distal4.312383.29312HepG2FXR2
chr149559645295596508-CDS,intron-Distal4.102889.37631HepG2BCLAF1
chr149559580895595854-CDS,intron-Distal,intron-Proximal3.8499210.67609HepG2BCLAF1
chr149559304295593085-CDS,intron-Distal4.698869.14010HepG2BCLAF1
chr149559078495590840-CDS,intron-Distal3.765835.09222HepG2FXR2
chr149555756495557699-CDS,intron-Distal,intron-Proximal5.8180011.12069HepG2FXR2
chr149558395895584063-CDS,intron-Distal,intron-Proximal4.161093.04254K562UCHL5
chr149557136395571433-CDS,intron-Distal,intron-Proximal5.689696.69228HepG2PRPF8
chr149558397695584063-CDS,intron-Distal3.549055.31167HepG2GRWD1
chr149556611795566155-CDS,intron-Distal4.099415.94293HepG2BCLAF1
chr149558295395583033-CDS,intron-Distal,intron-Proximal5.558319.32708HepG2FXR2
chr149556993195570006-CDS,intron-Distal,intron-Proximal3.200198.71292HepG2BCLAF1
chr149556039495560492-CDS,intron-Distal3.217415.74981HepG2GRWD1
chr149559052995590645-CDS,intron-Distal,intron-Proximal4.6970329.84980HepG2BCLAF1
chr149557764995577734-CDS,intron-Distal,intron-Proximal4.098433.88780HepG2G3BP1
chr149558286295582927-CDS,intron-Distal,intron-Proximal4.5451413.52497HepG2BCLAF1
chr149556291195563046-CDS,intron-Distal4.2742726.70106HepG2BCLAF1
chr149555758095557651-CDS,intron-Distal3.301157.17439HepG2BCLAF1
chr149559076595590871-CDS,intron-Distal3.8963921.20562HepG2BCLAF1
chr149558404795584083-CDS,intron-Distal4.229627.09561HepG2BCLAF1
chr149559053395590576-CDS,intron-Distal3.563633.88337HepG2FXR2
chr149558281695582899-CDS,intron-Distal3.508124.04703HepG2FXR2
chr149559078195590879-CDS,intron-Distal3.616984.22136HepG2RBM15
chr149558200895582064-CDS,intron-Distal3.902243.56019HepG2GRWD1
chr149557009595570138-CDS,intron-Distal3.718923.48848HepG2FXR2
chr149558278795582831-CDS,intron-Distal,intron-Proximal4.4091011.11870HepG2BCLAF1
chr149559885595598888-CDS,intron-Distal,intron-Proximal5.228307.73487HepG2BCLAF1
chr149556291895563036-CDS,intron-Distal3.289713.89474HepG2GRWD1
chr149558292795583030-CDS,intron-Distal,intron-Proximal4.0794220.16822HepG2BCLAF1
chr149557765895577768-CDS,intron-Distal3.834588.60107HepG2FXR2
chr149559299095593042-CDS,intron-Distal4.742599.80377HepG2BCLAF1
chr149556620595566256-CDS,intron-Distal4.391898.47938HepG2BCLAF1
chr149556289895563026-CDS,intron-Distal3.865925.97760HepG2G3BP1
chr149557765395577733-CDS,intron-Distal,intron-Proximal3.4097012.67896HepG2BCLAF1
chr149555739195557412-CDS,intron-Distal3.837625.06180HepG2BCLAF1
chr149556292595563045-CDS,intron-Distal3.555408.46997HepG2PPIG
chr149556293895563021-CDS,intron-Distal3.328413.54210HepG2EIF3H
chr149559092995590998-CDS,intron-Distal5.8041811.17302HepG2BCLAF1
chr149558203095582066-CDS,intron-Distal4.249623.42472HepG2FXR2
chr149559294395592972-CDS,intron-Distal4.842125.68196HepG2BCLAF1
chr149558408395584091-CDS,intron-Distal5.406389.32793HepG2BCLAF1
chr149557246595572514-CDS,intron-Distal,intron-Proximal5.5207910.47286HepG2BCLAF1
chr149557479495574836-CDS,intron-Distal3.241307.13168HepG2BCLAF1
chr149558202195582051-CDS,intron-Distal3.113903.55800HepG2BCLAF1
chr149557400795574039-CDS,intron-Distal3.372824.34695HepG2BCLAF1
chr149561783495617999-intron-Distal3.618924.94813HepG2HNRNPM
chr149562186995621928-intron-Distal5.040157.87558HepG2HNRNPM
chr149562178095621843-intron-Distal4.093613.62366HepG2HNRNPM
chr149562267795622776-intron-Distal3.903064.94813HepG2HNRNPM
chr149560335595603484-intron-Distal4.7328410.84081HepG2PTBP1
chr149562098295621088-intron-Distal4.572444.80665HepG2QKI
chr149559181895592014-intron-Distal6.2173817.22982HepG2PCBP2
chr149560333295603437-intron-Distal5.9154313.54105HepG2PCBP2
chr149559852895598574-intron-Distal4.869744.20763HepG2U2AF2
chr149560381195603887-intron-Distal5.500907.34252HepG2PCBP2
chr149559211295592198-intron-Distal4.384323.28841HepG2PTBP1
chr149561742195617509-intron-Distal3.441015.10768HepG2QKI
chr149562379195623848-intron-Distal,intron-Proximal5.719686.02643HepG2DDX3X
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DICER1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DICER1 is not available on ENCORE database.

  • 96 RBPs can affect the levels of DICER1.  








The Knockdown/knockout RNA-Seq assay for DICER1 is not available on ENCORE database.





96 RBPs can affect the gene expression of DICER1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DICER1ENSG00000100697.102394.814901916.093900.321602.774001.69930HepG2AATF
DICER1ENSG00000100697.102043.521102723.09160-0.414207.853006.64080HepG2AKAP8L
DICER1ENSG00000100697.103307.484103746.84670-0.179902.143301.52550HepG2AUH
DICER1ENSG00000100697.101030.830402423.04630-1.2330020.1797017.54520HepG2BCCIP
DICER1ENSG00000100697.103094.412404116.59740-0.411802.188301.33010HepG2CCAR1
DICER1ENSG00000100697.101460.661102653.16050-0.861107.314706.07650HepG2CNOT7
DICER1ENSG00000100697.101700.952002298.78820-0.434506.417004.87120HepG2YBX3
DICER1ENSG00000100697.101862.034501496.804100.314902.607701.55950HepG2CSTF2
DICER1ENSG00000100697.101793.755302730.87040-0.6064010.083608.80800HepG2DAZAP1
DICER1ENSG00000100697.103725.015102656.199200.487804.826204.01820HepG2DDX3X
DICER1ENSG00000100697.101867.583702382.31950-0.351202.133701.44650HepG2DDX55
DICER1ENSG00000100697.102073.329403234.35120-0.641503.795902.65630HepG2DDX59
DICER1ENSG00000100697.102268.213102593.02890-0.193102.112901.50850HepG2DKC1
DICER1ENSG00000100697.101499.833702257.30490-0.5898012.1237010.73460HepG2DNAJC21
DICER1ENSG00000100697.101640.532702237.27470-0.4476010.772709.58300HepG2DNAJC2
DICER1ENSG00000100697.102525.664301996.703000.338903.608502.39560HepG2EIF3G
DICER1ENSG00000100697.101888.610302648.51090-0.487906.511905.23210HepG2EWSR1
DICER1ENSG00000100697.102564.882502015.048400.348003.423602.17160HepG2FASTKD2
DICER1ENSG00000100697.101969.894901186.250600.7316011.8333010.26240HepG2FUBP3
DICER1ENSG00000100697.103002.033102268.794200.403903.238002.52090HepG2G3BP1
DICER1ENSG00000100697.103611.161202408.450800.584307.111405.67010HepG2G3BP2
DICER1ENSG00000100697.101696.333202278.92940-0.425903.754103.01840HepG2HNRNPA1
DICER1ENSG00000100697.102961.768102297.064700.366603.412502.63970HepG2HNRNPK
DICER1ENSG00000100697.101888.404502518.09880-0.415208.149707.14880HepG2HNRNPU
DICER1ENSG00000100697.103388.676002306.224700.555106.135605.16710HepG2IGF2BP2
DICER1ENSG00000100697.103659.955002164.111300.758002.680201.71320HepG2IGF2BP3
DICER1ENSG00000100697.102109.555502572.16010-0.286005.334004.34220HepG2ILF2
DICER1ENSG00000100697.103250.237102319.583900.486603.639702.47050HepG2KHSRP
DICER1ENSG00000100697.102041.438203353.03240-0.715905.785603.96910HepG2KRR1
DICER1ENSG00000100697.103909.905302460.200900.668307.604506.59800HepG2MATR3
DICER1ENSG00000100697.103948.237902518.184100.648707.749806.71610HepG2NCBP2
DICER1ENSG00000100697.103116.195602242.753100.474404.461003.55730HepG2PABPC4
DICER1ENSG00000100697.102579.545702174.854900.246103.093502.45760HepG2PCBP1
DICER1ENSG00000100697.101912.445202245.94850-0.231902.368201.68140HepG2PPIL4
DICER1ENSG00000100697.103337.533002378.237700.4888010.976609.58560HepG2PRPF4
DICER1ENSG00000100697.101035.620701837.97790-0.8276010.720709.26960HepG2PRPF8
DICER1ENSG00000100697.102934.556102212.941700.407107.235606.43240HepG2PTBP1
DICER1ENSG00000100697.103355.067302741.802300.291102.917902.22930HepG2PUM2
DICER1ENSG00000100697.104062.905202396.786800.7613020.2149018.74950HepG2QKI
DICER1ENSG00000100697.102212.193403595.51120-0.7007019.1452018.03220HepG2RBM22
DICER1ENSG00000100697.103953.708402175.194100.8620020.2566018.54430HepG2RBM25
DICER1ENSG00000100697.103703.106802462.836900.5883015.9293014.83310HepG2NELFE
DICER1ENSG00000100697.103109.318702293.715700.438809.155608.13360HepG2RECQL
DICER1ENSG00000100697.102578.914303108.86600-0.269602.482301.82980HepG2RPLP0
DICER1ENSG00000100697.102029.137002663.73940-0.392602.360201.61850HepG2RPS3A
DICER1ENSG00000100697.101639.354402242.49740-0.452009.965908.86010HepG2SAFB2
DICER1ENSG00000100697.103335.594902304.055800.5337013.2731012.12870HepG2SF3B4
DICER1ENSG00000100697.102968.115802478.078500.260203.450802.68170HepG2SLBP
DICER1ENSG00000100697.102102.851602615.16300-0.314606.581605.65490HepG2SMN1
DICER1ENSG00000100697.102613.946504061.03610-0.6356022.3414021.27390HepG2SRFBP1
DICER1ENSG00000100697.104576.834202404.895400.9283044.8690043.47060HepG2SRSF1
DICER1ENSG00000100697.102280.143302835.19250-0.314305.335504.42200HepG2SRSF7
DICER1ENSG00000100697.102962.465301956.476100.5984017.3336016.16720HepG2SRSF9
DICER1ENSG00000100697.104366.747903526.008300.308406.052205.34850HepG2SSB
DICER1ENSG00000100697.101776.841102317.01110-0.383002.337101.34020HepG2STIP1
DICER1ENSG00000100697.103627.197102902.389200.321504.631603.86410HepG2SUCLG1
DICER1ENSG00000100697.103386.472702161.503000.6476015.5973014.42830HepG2TAF15
DICER1ENSG00000100697.102696.066901986.244400.440705.871304.66560HepG2TIA1
DICER1ENSG00000100697.101718.973702446.92480-0.509408.060107.28740HepG2TIAL1
DICER1ENSG00000100697.101887.246602468.66830-0.387507.167806.33360HepG2TRIM56
DICER1ENSG00000100697.103101.504202524.965800.296602.724101.85790HepG2TUFM
DICER1ENSG00000100697.10928.613601401.03280-0.593305.730304.62790HepG2UCHL5
DICER1ENSG00000100697.101087.621002171.84220-0.9977010.103608.12580HepG2UPF2
DICER1ENSG00000100697.102830.327203400.07930-0.264603.722702.52630HepG2UTP18
DICER1ENSG00000100697.102584.382203193.65340-0.305405.278104.49560HepG2UTP3
DICER1ENSG00000100697.10858.234502334.60140-1.4437032.1530029.82420HepG2XRN2
DICER1ENSG00000100697.103302.913802347.060300.4928014.4210013.08240HepG2ZC3H8
DICER1ENSG00000100697.104010.939603638.062700.140701.959801.47780HepG2ZNF622
DICER1ENSG00000100697.10805.653101375.04980-0.7713016.8201015.54460K562AGGF1
DICER1ENSG00000100697.10952.256201510.89190-0.666009.074507.00970K562AKAP8
DICER1ENSG00000100697.10632.430701430.78200-1.1778023.8162021.55170K562AKAP8L
DICER1ENSG00000100697.10992.255701258.82070-0.343302.464901.90460K562AQR
DICER1ENSG00000100697.10976.922001501.19760-0.6198010.824908.87020K562ATP5C1
DICER1ENSG00000100697.10655.44780858.46470-0.389303.582302.04200K562BCCIP
DICER1ENSG00000100697.101328.191101643.32380-0.307202.976401.74530K562CCDC124
DICER1ENSG00000100697.101042.473101526.12130-0.549902.852001.76940K562CNOT7
DICER1ENSG00000100697.101208.048301526.07730-0.337103.703702.41330K562CPEB4
DICER1ENSG00000100697.10499.09720718.71270-0.526105.642503.90450K562EIF4B
DICER1ENSG00000100697.10723.639601180.94730-0.706608.224506.67780K562FIP1L1
DICER1ENSG00000100697.101272.45230890.226500.515303.463902.65500K562G3BP2
DICER1ENSG00000100697.101022.531001438.03570-0.492005.366604.30900K562GRSF1
DICER1ENSG00000100697.101164.284001518.48620-0.383204.074903.04700K562KIF1C
DICER1ENSG00000100697.101045.832601453.50900-0.474904.731103.21110K562MAK16
DICER1ENSG00000100697.101195.349701701.07850-0.509006.888305.32710K562MBNL1
DICER1ENSG00000100697.101265.555201046.923800.273502.812901.99700K562MTPAP
DICER1ENSG00000100697.10886.772501192.32680-0.427102.500801.67140K562NAA15
DICER1ENSG00000100697.10618.680101070.37020-0.790803.778902.57180K562PABPN1
DICER1ENSG00000100697.10489.79890661.91840-0.434502.744801.33130K562PHF6
DICER1ENSG00000100697.10739.172501340.52560-0.858809.122407.40560K562PPIG
DICER1ENSG00000100697.10522.47200740.58490-0.503303.498002.65370K562PRPF8
DICER1ENSG00000100697.10430.52050607.88540-0.497703.153102.17880K562PSIP1
DICER1ENSG00000100697.10869.519401419.08390-0.706709.145508.20630K562PUS1
DICER1ENSG00000100697.10888.036801382.56680-0.6387010.048908.94450K562RBM15
DICER1ENSG00000100697.10669.83140964.23770-0.525604.946203.78250K562RBM22
DICER1ENSG00000100697.10946.309701390.04380-0.554702.982701.40480K562SBDS
DICER1ENSG00000100697.10743.43890925.89280-0.316602.519501.70560K562SF3B4
DICER1ENSG00000100697.10659.352101194.09000-0.856804.050702.35340K562SFPQ
DICER1ENSG00000100697.10902.775801175.92760-0.381405.203103.96480K562SRPK2
DICER1ENSG00000100697.10616.90510997.53730-0.693306.508405.38100K562TARDBP
DICER1ENSG00000100697.10747.00430962.21310-0.365203.064202.19490K562TIA1
DICER1ENSG00000100697.10738.27280943.99820-0.354602.473301.45280K562TRA2A
DICER1ENSG00000100697.10856.341901055.41470-0.301602.254001.46520K562WDR3
DICER1ENSG00000100697.101282.592401097.473300.224802.209201.44540K562WDR43
DICER1ENSG00000100697.101196.563501491.48030-0.317802.654401.39500K562WRN
DICER1ENSG00000100697.10436.79630772.75750-0.823108.526406.08840K562XRN2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DICER1 is not available on ENCORE database.





5 RBPs can affect the splicing of DICER1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEDICER1ENSG00000100697.10chr14-955958089559596995592916955930859559639495596529-0.197000.001210.02418excludedHepG2U2AF1
SEDICER1ENSG00000100697.10chr14-955978459559797695596394955965299559885195599014-0.664000.000000.00008excludedK562PUS1
SEDICER1ENSG00000100697.10chr14-955978459559797695596394955965299559885195599014-0.521000.000810.02321excludedK562RBM15
SEDICER1ENSG00000100697.10chr14-955978459559797695596394955965299559885195599014-0.615000.000030.00247excludedK562HNRNPK
A3SSDICER1ENSG00000100697.10chr14-9555682895557019955568289555700095557370955574460.120000.007710.04222longerK562AQR
A3SSDICER1ENSG00000100697.10chr14-9559580895595987955958089559596995596394955965290.237000.007430.04091longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DICER1 is not avaliable on ENCORE database.