APTX

General Information

Gene Name       : APTX
Gene ID             : ENSG00000137074.18
Essential Gene : No

Functions

Localization

Domains (AlphaFold: APTX)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878APTXENSG00000137074.1844.2639.7141.98ENCSR000AEF
HepG2APTXENSG00000137074.1828.9540.5134.73ENCSR985KAT
K562APTXENSG00000137074.1842.0149.4745.74ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq APTX-BGKcLV23-K562ENCSR148HLAAPTXK562ReleasedENCSR877JEM   CRISPRKD% = 71.0,78.0264144
knockdown followed by RNA-seq APTX-BGHcLV24-HepG2ENCSR489DNHAPTXHepG2ReleasedENCSR671FDZ   CRISPRKD% = 76.0,77.0






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
APTXENCAB069JSSPA5-92422UK2881303AReleased, ENCODE4BGHcLV24-11, Released, ENCODE4BGKcLV23-15, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
APTXK562CRISPRENCSR148HLAENCSR877JEMAPTX-BGKcLV23-15,APTX-BGKcLV23-16NT-BGKcLV23-1,NT-BGKcLV23-271.0,78.0264144ReleasedENCODE4
APTXK562CRISPRBGKcLV23-17,BGKcLV23-18BGKcLV23-1,BGKcLV23-261.0,62.7NotSatisfiedENCODE4
APTXHepG2CRISPRENCSR489DNHENCSR671FDZAPTX-BGHcLV24-11,APTX-BGHcLV24-12NT-BGHcLV24-1,NT-BGHcLV24-276.0,77.0ReleasedENCODE4
APTXHepG2CRISPRBGHcLV24-13,BGHcLV24-14BGHcLV24-1,BGHcLV24-271.0,74.0NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
APTXProduct_ID: PA5-92422
Lot_ID: UK2881303A
Source: Thermo Fisher
Target Name: APTX-human		 APTX-HEPG2-CRISPR-PA5-92422.png<br>Caption: Western blot following CRISPR against APTX in HepG2 whole cell lysate using APTX specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against APTX. APTX protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.
 ThermoFisher_PA5-92422_UK2881303A_APTX.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the APTX specific antibody, PA5-92422. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the APTX-specific antibody, PA5-92422. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 APTX-K562-CRISPR-PA5-92422.png<br>Caption: Western blot following CRISPR against APTX in K562 whole cell lysate using APTX specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against APTX. APTX protein appears as the green arrow, Tubulin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for APTX is not avaliable on ENCORE database.



Proteins bound to RNA
  • APTX RNA is bound by 17 proteins.  







eCLIP assay for APTX is not avaliable on ENCORE database.





17 proteins are bound to APTX RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr93298598132986035-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.206923.93230HepG2U2AF1
chr93298597232986036-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal4.2013414.20290HepG2U2AF2
chr93298598032986036-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal5.4108313.76205K562U2AF2
chr93298596732986033-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.614914.97821K562BUD13
chr93298779732987861-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.116374.02686HepG2U2AF2
chr93298598632986035-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal5.158987.45412K562U2AF1
chr93298596232986019-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.349943.05460HepG2PPIG
chr93298597432986030-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.205793.31745K562ZNF622
chr93298982532989894-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.320418.73886K562DDX3X
chr93298760632987658-3'-UTR,5'-UTR,CDS,intron-Distal3.045143.55800HepG2BCLAF1
chr93298983132989900-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.435094.57751HepG2U2AF2
chr93302492133025016-5'-UTR,intron-Distal,intron-Proximal3.743367.64728K562AQR
chr93299173532991779-intron-Distal3.503775.06177HepG2SUGP2
chr93299754232997628-intron-Distal4.292113.09122HepG2PTBP1
chr93299814232998200-intron-Distal3.671785.30446HepG2SUGP2
chr93299779332997861-intron-Distal3.214503.69184HepG2SUGP2
chr93299788932997942-intron-Distal4.118183.86809HepG2SUGP2
chr93300373033003780-intron-Distal3.922643.57189K562ILF3
chr93302240933022469-intron-Distal3.918093.49011K562KHSRP
chr93298156532981653-intron-Distal4.411833.70058HepG2XPO5
chr93299923632999350-intron-Distal4.840594.74221HepG2PTBP1
chr93302264733022775-intron-Distal4.039394.06464HepG2CSTF2
chr93301896133019024-intron-Distal5.362308.57494HepG2U2AF2
chr93297435032974442-intron-Distal,intron-Proximal3.788114.95247K562PRPF8
chr93299227632992319-intron-Distal3.977533.04224HepG2SUGP2
chr93299924232999330-intron-Distal3.878246.49112K562PTBP1
chr93302286033022906-intron-Distal3.955563.24593HepG2XPO5
chr93301021933010349-intron-Distal4.079855.14628K562TARDBP
chr93299923632999357-intron-Distal4.396755.04209K562MATR3
chr93299211832992203-intron-Distal4.732316.36848HepG2SUGP2
chr93299279032992857-intron-Distal4.582985.04879HepG2SUGP2
chr93299904932999150-intron-Distal4.992008.56566HepG2SUGP2
chr93299240032992455-intron-Distal3.351014.33009HepG2SUGP2
chr93299892132998972-intron-Distal3.946833.54208HepG2SUGP2
chr93299832832998371-intron-Distal3.025973.23296HepG2SUGP2
chr93300386133003947-intron-Distal3.966583.81649K562ILF3
chr93297675032976974-intron-Distal3.338824.35650K562KHSRP
chr93299790332998035-intron-Distal4.611326.01663K562MATR3
chr93298964232989752-intron-Distal,intron-Proximal3.384433.47285K562PRPF8
chr93299925532999321-intron-Distal4.611673.27829HepG2MATR3
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for APTX is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for APTX is not available on ENCORE database.

  • 57 RBPs can affect the levels of APTX.  








The Knockdown/knockout RNA-Seq assay for APTX is not available on ENCORE database.





57 RBPs can affect the gene expression of APTX :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
APTXENSG00000137074.14627.87280465.448400.431802.184001.34320HepG2ABCF1
APTXENSG00000137074.14632.28010444.762900.507405.518904.71880HepG2ACO1
APTXENSG00000137074.141070.92630821.003400.383302.145401.67080HepG2AKAP1
APTXENSG00000137074.14328.94640467.67080-0.507602.466701.61850HepG2BCLAF1
APTXENSG00000137074.14553.77240392.237800.497503.990003.08720HepG2CALR
APTXENSG00000137074.14194.47000375.55150-0.949507.419206.33610HepG2CPSF6
APTXENSG00000137074.14526.78780411.330800.356802.976302.11290HepG2DDX19B
APTXENSG00000137074.14631.77060439.987900.521802.255101.63480HepG2DDX21
APTXENSG00000137074.14384.35250649.42580-0.756706.407905.52620HepG2EIF2S1
APTXENSG00000137074.14963.67050717.654000.425202.619801.99680HepG2EIF4A3
APTXENSG00000137074.14570.14800434.591200.391602.587301.78070HepG2EWSR1
APTXENSG00000137074.14267.64650360.62260-0.430202.223401.51190HepG2FUBP3
APTXENSG00000137074.14497.13830619.74210-0.318002.416101.74260HepG2HNRNPF
APTXENSG00000137074.14507.91710680.33730-0.421702.659301.97410HepG2HNRNPK
APTXENSG00000137074.14527.67140655.47880-0.312902.574401.92290HepG2HNRNPL
APTXENSG00000137074.141205.97080728.486800.727106.796205.84130HepG2MATR3
APTXENSG00000137074.14295.90540392.85380-0.408902.550001.83900HepG2PPIL4
APTXENSG00000137074.14497.61130647.98810-0.380902.748202.20200HepG2PTBP1
APTXENSG00000137074.14518.01550681.14770-0.395002.666702.07960HepG2PUF60
APTXENSG00000137074.141189.63520696.458900.7723013.6795012.70200HepG2RBM22
APTXENSG00000137074.14493.39420636.88130-0.368302.334701.70980HepG2RBM25
APTXENSG00000137074.141006.54940667.630900.592205.958005.01210HepG2RPLP0
APTXENSG00000137074.14348.04380504.14000-0.534602.506701.60550HepG2RPS10
APTXENSG00000137074.14307.52200392.24500-0.351102.139901.56060HepG2SAFB2
APTXENSG00000137074.14501.73900653.13570-0.380403.409902.64490HepG2SLBP
APTXENSG00000137074.14999.90320786.621200.346003.747303.19130HepG2SRFBP1
APTXENSG00000137074.14892.72120704.141400.342203.340602.83330HepG2SRSF1
APTXENSG00000137074.141001.87810682.967300.552708.354707.55710HepG2SSB
APTXENSG00000137074.14881.36430427.963701.0422018.2227017.14790HepG2TIAL1
APTXENSG00000137074.14630.27950414.660800.604006.584805.72760HepG2UTP3
APTXENSG00000137074.14691.96740846.75820-0.291202.436801.89880K562AGGF1
APTXENSG00000137074.14991.180101512.60350-0.609806.690805.84250K562AQR
APTXENSG00000137074.14755.37870949.72050-0.330302.895301.91480K562BCLAF1
APTXENSG00000137074.14371.31820508.17590-0.452702.524901.69320K562BOP1
APTXENSG00000137074.14330.95300540.35160-0.707306.624505.40560K562CPSF6
APTXENSG00000137074.14321.79390442.10680-0.458302.646701.35110K562DHX30
APTXENSG00000137074.14504.37100824.41310-0.708906.846705.35740K562EFTUD2
APTXENSG00000137074.14773.598501109.07900-0.519707.079306.00650K562EIF2S2
APTXENSG00000137074.14543.48850882.79770-0.699805.652604.10300K562EIF3D
APTXENSG00000137074.141239.500901650.89450-0.413505.198603.92920K562EIF4G2
APTXENSG00000137074.14407.61450549.76620-0.431602.532201.71430K562FMR1
APTXENSG00000137074.14339.54520465.96400-0.456602.526401.65650K562FTO
APTXENSG00000137074.14827.25730621.509500.412503.971203.26570K562FXR1
APTXENSG00000137074.14368.45660515.91460-0.485603.143402.29940K562GPKOW
APTXENSG00000137074.14559.92410772.53710-0.464403.753703.07090K562HNRNPC
APTXENSG00000137074.14460.11300689.90950-0.584405.102303.48910K562HNRNPF
APTXENSG00000137074.141098.53340670.286000.712606.954305.37370K562HNRNPM
APTXENSG00000137074.14857.677001147.34020-0.419804.762103.58190K562HNRNPU
APTXENSG00000137074.14299.43510440.49570-0.556902.711501.78970K562KRR1
APTXENSG00000137074.141035.08850827.413700.323002.499301.62590K562MATR3
APTXENSG00000137074.14450.40030633.91680-0.493102.184301.47270K562POLR2G
APTXENSG00000137074.14549.49640802.24630-0.545904.141803.22450K562PRPF8
APTXENSG00000137074.141414.112501801.25080-0.349102.750001.93640K562PUF60
APTXENSG00000137074.14569.24270817.25140-0.521703.183902.30590K562RPS19
APTXENSG00000137074.14714.59270956.78390-0.421104.634003.60140K562SF3A3
APTXENSG00000137074.14303.35140490.60990-0.693604.534203.27320K562SF3B1
APTXENSG00000137074.14420.64580599.12910-0.510303.591402.37890K562SRFBP1
APTXENSG00000137074.14814.33410980.24160-0.267502.580601.67120K562SRPK2
APTXENSG00000137074.14865.714801028.17320-0.248102.340801.67700K562UPF1
APTXENSG00000137074.14716.35640879.77930-0.296502.075201.31880K562WDR3
APTXENSG00000137074.14725.45720914.84100-0.334603.636702.67530K562WDR43
APTXENSG00000137074.14590.15180837.09040-0.504303.743402.26130K562XRN2
APTXENSG00000137074.141299.341001704.06740-0.391203.761302.72640K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for APTX is not available on ENCORE database.





20 RBPs can affect the splicing of APTX :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.647000.000000.00000excludedK562SF3B4
SEAPTXENSG00000137074.14chr9-329846283298485532974455329745593298596832986028-0.081000.002180.06626excludedK562PRPF8
SEAPTXENSG00000137074.14chr9-329880803298812732987541329877293298975632990086-0.292000.000000.00000excludedK562AKAP8L
SEAPTXENSG00000137074.14chr9-3298808032988127329875413298772932989756329898930.191000.003400.03889includedHepG2U2AF2
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632989893-0.169000.008140.07772excludedHepG2U2AF2
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632989893-0.312000.000140.00227excludedK562AQR
SEAPTXENSG00000137074.14chr9-329880803298812732987541329877293298975632990086-0.212000.000030.00078excludedK562PUS1
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632989893-0.335000.000180.01395excludedK562EFTUD2
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.223000.003370.06008excludedK562U2AF1
SEAPTXENSG00000137074.14chr9-329880803298812732987541329877293298975632990086-0.141000.001520.08574excludedK562HNRNPL
SEAPTXENSG00000137074.14chr9-329880803298812732987541329877293298975632990086-0.198000.000380.08757excludedHepG2CDC40
SEAPTXENSG00000137074.14chr9-3298808032988127329875413298772932989756329898930.199000.000680.07504includedHepG2HNRNPUL1
SEAPTXENSG00000137074.14chr9-3298808032988127329875413298772932989756329900860.241000.000130.01827includedHepG2SUPV3L1
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.409000.000060.00482excludedHepG2SF3B4
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.306000.000150.00413excludedK562U2AF2
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.270000.001210.04356excludedK562TARDBP
SEAPTXENSG00000137074.14chr9-329880803298812232987541329877293298975632989917-0.141000.003550.07047excludedK562RBM15
SEAPTXENSG00000137074.14chr9-329880803298812732987541329878443298975632990086-0.249000.000810.03422excludedK562SF3B1
SEAPTXENSG00000137074.14chr9-329846283298485532974455329745593298596832986028-0.135000.000500.01067excludedK562HNRNPC
A3SSAPTXENSG00000137074.14chr9-329875413298784432987541329877293298808032988127-0.279000.000000.00641shorterHepG2DDX59
A3SSAPTXENSG00000137074.14chr9-329875413298784432987541329877293298975632989893-0.214000.000220.02449shorterK562AKAP8L
A3SSAPTXENSG00000137074.14chr9-329880763298812732988076329881223298975632990086-0.407000.000070.05901shorterHepG2RBM5
A3SSAPTXENSG00000137074.14chr9-3298754132987844329875413298772932989756329899170.215000.001910.06279longerK562U2AF2
A3SSAPTXENSG00000137074.14chr9-3298754132987844329875413298772932989756329898930.243000.000200.01502longerK562SF3B4
A3SSAPTXENSG00000137074.14chr9-3298754132987844329875413298772932989756329898930.138000.004810.09304longerHepG2U2AF2
A3SSAPTXENSG00000137074.14chr9-329875413298784432987541329877293298808032988127-0.296000.000040.02543shorterHepG2DDX6
A3SSAPTXENSG00000137074.14chr9-3298975632989917329897563298989333024997330251660.505000.000000.00000longerK562AQR
A3SSAPTXENSG00000137074.14chr9-3298754132987844329875413298772932988080329881270.215000.000290.01650longerK562U2AF1
A3SSAPTXENSG00000137074.14chr9-329875413298784432987541329877293298808032988127-0.152000.000830.08543shorterK562HNRNPC
MXEAPTXENSG00000137074.14chr9-3298754132987844329880803298812732985968329860283298975632989893-0.128000.003620.027452includedK562AQR
MXEAPTXENSG00000137074.14chr9-3298754132987844329880803298812232985968329860283298975632989893-0.133000.004450.032532includedK562AQR
MXEAPTXENSG00000137074.14chr9-32987541329878443298808032988127329859683298602832989756329898930.130000.000850.066781includedK562PUM1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for APTX is not avaliable on ENCORE database.