EIF2AK4

General Information

Gene Name       : EIF2AK4
Gene ID             : ENSG00000128829.11
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: EIF2AK4)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878EIF2AK4ENSG00000128829.1121.5419.5920.56ENCSR000AEF
HepG2EIF2AK4ENSG00000128829.1138.3851.0444.71ENCSR985KAT
K562EIF2AK4ENSG00000128829.1122.0025.1223.56ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
EIF2AK465981S1Submitted, ENCORE2BGKcLV42-31, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
EIF2AK4K562CRISPRBGKcLV38-39,BGKcLV38-40BGKcLV38-1,BGKcLV38-20.0,0.0NotSatisfiedENCORE2
EIF2AK4K562CRISPREIF2AK4-BGKcLV42-31,EIF2AK4-BGKcLV42-32NT-BGKcLV42-1,NT-BGKcLV42-270.3,74.9SequencedENCORE2
EIF2AK4HepG2CRISPRBGHcLV39-47,BGHcLV39-48BGHcLV39-1,BGHcLV39-20.0,0.0NotSatisfiedENCORE2
EIF2AK4HepG2CRISPRBGHcLV43-19,BGHcLV43-20BGHcLV43-1,BGHcLV43-2NO BAND ON THE POSITION,NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2AK4Product_ID: 65981S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF2AK4-human		 CST_65981S_1_EIF2AK4.png<br>Caption: IP-WB analysis of 65981S whole cell lysate using the EIF2AK4 specific antibody, 65981S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2AK4-specific antibody, 65981S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF2AK4-K562-CRISPR-65981S.png<br>Caption: Western blot following CRISPR against EIF2AK4 in K562 whole cell lysate using EIF2AK4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK4. EIF2AK4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for EIF2AK4 is not avaliable on ENCORE database.



Proteins bound to RNA
  • EIF2AK4 RNA is bound by 19 proteins.  







eCLIP assay for EIF2AK4 is not avaliable on ENCORE database.





19 proteins are bound to EIF2AK4 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr154022637540226463+5'-UTR,CDS3.697156.83172K562FXR2
chr154022636840226439+5'-UTR,CDS4.6902321.64325HepG2DDX3X
chr154022637740226481+5'-UTR,CDS5.003255.47892K562DDX3X
chr154022637440226452+5'-UTR,CDS4.056446.83513K562FMR1
chr154022639340226455+5'-UTR,CDS4.041143.43980K562ZNF622
chr154030870240308730+CDS,intron-Distal,intron-Proximal4.548984.78556K562LIN28B
chr154029333240293407+CDS3.044733.55392K562YBX3
chr154024612140246174+CDS4.341833.71264HepG2FXR2
chr154030938740309434+CDS4.023405.94293HepG2BCLAF1
chr154025797740258035+CDS4.383478.41475HepG2BCLAF1
chr154023563640235687+CDS,intron-Proximal3.8105712.60692HepG2BCLAF1
chr154025954440259633+CDS,intron-Proximal4.0177210.78406HepG2BCLAF1
chr154023558340235603+CDS,intron-Proximal4.980335.23259HepG2BCLAF1
chr154031821940318286+CDS4.346599.80976HepG2BCLAF1
chr154030183540301914+CDS4.2937711.64153HepG2BCLAF1
chr154032257540322645+CDS4.107184.69071HepG2GRWD1
chr154030353040303548+CDS,intron-Proximal4.951147.42079K562LIN28B
chr154026861540268664+CDS3.168164.79538HepG2BCLAF1
chr154032256540322655+CDS3.265373.87559K562YBX3
chr154024790940247963+CDS4.073843.31827HepG2GRWD1
chr154031823040318291+CDS,intron-Proximal3.161093.12941K562UCHL5
chr154023563740235687+CDS,intron-Proximal3.267183.05161HepG2PPIG
chr154026581740265882+CDS5.030866.23448K562FXR2
chr154032264940322694+CDS,intron-Proximal3.588365.35401HepG2PRPF8
chr154031477440314844+CDS,intron-Proximal4.976195.35117HepG2PRPF8
chr154024140740241469+CDS3.436843.66187K562ZNF622
chr154030942940309466+CDS,intron-Proximal5.041817.02967HepG2PRPF8
chr154024131840241365+CDS4.076284.01763HepG2GRWD1
chr154025789340258045+CDS,intron-Proximal3.531734.70460K562DDX24
chr154028021240280266+CDS3.849043.65750K562YBX3
chr154030031140300374+CDS3.321473.68829K562YBX3
chr154026863340268707+CDS4.741595.24786K562FXR2
chr154024782440247891+CDS5.5835510.71076HepG2BCLAF1
chr154024131440241420+CDS,intron-Proximal3.7995111.07607K562UCHL5
chr154032160640321663+CDS,intron-Proximal3.044424.89002HepG2BCLAF1
chr154026865440268722+CDS4.519904.56100HepG2FXR2
chr154030930740309374+CDS,intron-Proximal3.8303811.10779HepG2BCLAF1
chr154023562540235685+CDS3.542294.45064HepG2GRWD1
chr154026890240269041+CDS4.6469513.19831K562FXR2
chr154026893540269029+CDS4.321707.51229K562FMR1
chr154030870340308731+CDS,intron-Distal3.580345.97823HepG2BCLAF1
chr154025399340254083+CDS4.443306.01390HepG2GRWD1
chr154024610440246187+CDS,intron-Proximal3.824315.80935K562ZNF622
chr154023566440235686+CDS3.478935.53307K562ZNF622
chr154030351440303530+CDS4.671836.32937K562LIN28B
chr154030873140308776+CDS,intron-Distal4.335378.15024HepG2BCLAF1
chr154031818240318286+CDS3.262715.33987HepG2GRWD1
chr154028254340282605+CDS,intron-Proximal4.743569.68077HepG2PRPF8
chr154026865140268703+CDS4.680156.76290HepG2GRWD1
chr154031817940318240+CDS3.851993.69113K562LIN28B
chr154030879640308875+CDS,intron-Distal,intron-Proximal3.5853311.34372HepG2BCLAF1
chr154032655540326599+CDS3.395363.94979HepG2BCLAF1
chr154030933940309444+CDS,intron-Proximal3.884183.11149HepG2G3BP1
chr154031819340318287+CDS3.771414.80912HepG2PPIG
chr154029921940299245+CDS3.287863.55800HepG2BCLAF1
chr154024782040247904+CDS,intron-Proximal3.851244.49723K562ZNF800
chr154023565740235687+CDS,intron-Proximal3.296584.89070K562UCHL5
chr154032652540326555+CDS,intron-Proximal3.756603.68471HepG2BCLAF1
chr154028018240280268+CDS,intron-Proximal3.763013.80092K562FXR1
chr154029319340293254+CDS3.342143.46769K562YBX3
chr154023178840231819+CDS,intron-Proximal4.301817.97334HepG2BCLAF1
chr154024141740241465+CDS3.457143.48848HepG2FXR2
chr154025789640258025+CDS3.117365.46976K562UCHL5
chr154024794140248008+CDS,intron-Proximal4.245134.83899HepG2PRPF8
chr154024790740247967+CDS5.337628.40988HepG2BCLAF1
chr154031818140318283+CDS3.719876.05315HepG2FXR2
chr154026861540268725+CDS5.099807.60619K562UCHL5
chr154025995740260028+CDS3.320525.35431HepG2BCLAF1
chr154025790740257959+CDS3.254376.59678HepG2BCLAF1
chr154029491340294984+CDS,intron-Proximal3.467565.32768K562YBX3
chr154024610440246186+CDS,intron-Proximal3.585855.65349K562UCHL5
chr154025400540254099+CDS3.117364.30396K562UCHL5
chr154025789240257999+CDS3.556584.45064HepG2GRWD1
chr154030033040300399+CDS,intron-Proximal3.241284.02291HepG2GRWD1
chr154029550240295555+CDS,intron-Proximal3.852878.39123HepG2PRPF8
chr154030880840308871+CDS,intron-Distal4.300604.91614HepG2GRWD1
chr154026511340265160+CDS3.537596.06436HepG2BCLAF1
chr154030221940302280+intron-Distal4.656043.16986HepG2QKI
chr154030079840300868+intron-Distal3.209823.37567HepG2HNRNPK
chr154030915840309291+intron-Distal,intron-Proximal3.911973.22073HepG2KHSRP
chr154030075240300845+intron-Distal3.540358.73313HepG2RBFOX2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for EIF2AK4 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for EIF2AK4 is not available on ENCORE database.

  • 80 RBPs can affect the levels of EIF2AK4.  








The Knockdown/knockout RNA-Seq assay for EIF2AK4 is not available on ENCORE database.





80 RBPs can affect the gene expression of EIF2AK4 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
EIF2AK4ENSG00000128829.72796.878702280.237800.294502.336901.46890HepG2ABCF1
EIF2AK4ENSG00000128829.72438.174002043.290400.254803.264102.43860HepG2AKAP8L
EIF2AK4ENSG00000128829.73759.797802874.988800.387002.947202.13780HepG2ASCC1
EIF2AK4ENSG00000128829.72804.313803365.25250-0.263103.955803.16230HepG2AUH
EIF2AK4ENSG00000128829.71209.880301658.54320-0.455103.395902.40300HepG2BCLAF1
EIF2AK4ENSG00000128829.72280.652001842.176100.307903.134002.31730HepG2CALR
EIF2AK4ENSG00000128829.72799.156902033.264800.4611012.2390011.04120HepG2CKAP4
EIF2AK4ENSG00000128829.72058.365401715.590000.262701.883501.30440HepG2CPSF6
EIF2AK4ENSG00000128829.71883.799401179.021800.676009.121007.35330HepG2CSTF2
EIF2AK4ENSG00000128829.72201.703201880.964400.227003.095802.21740HepG2DDX19B
EIF2AK4ENSG00000128829.72687.270101888.323700.508905.185704.28550HepG2DDX21
EIF2AK4ENSG00000128829.71368.618101876.66590-0.455503.327902.48940HepG2DDX55
EIF2AK4ENSG00000128829.71391.967002858.62140-1.038208.324507.30770HepG2DDX6
EIF2AK4ENSG00000128829.71345.191501673.55480-0.315102.568101.62310HepG2DHX30
EIF2AK4ENSG00000128829.71134.123801735.16640-0.6135011.5926010.23330HepG2DNAJC21
EIF2AK4ENSG00000128829.71356.291301719.79340-0.342605.772004.88100HepG2DNAJC2
EIF2AK4ENSG00000128829.71434.600402748.77210-0.938107.115706.19690HepG2EIF2S1
EIF2AK4ENSG00000128829.73604.744202712.889200.410008.566107.24460HepG2EIF4G1
EIF2AK4ENSG00000128829.74341.087803863.801900.167902.012401.48180HepG2ETF1
EIF2AK4ENSG00000128829.72422.976301987.309800.285902.619201.80580HepG2EWSR1
EIF2AK4ENSG00000128829.72902.118702302.607100.333702.474201.74230HepG2FIP1L1
EIF2AK4ENSG00000128829.71271.822401743.76790-0.455304.807303.78580HepG2FUBP3
EIF2AK4ENSG00000128829.72061.329203064.23580-0.572003.694602.90350HepG2FXR1
EIF2AK4ENSG00000128829.72726.042501916.414200.508303.297802.07640HepG2GRSF1
EIF2AK4ENSG00000128829.72094.711502527.13860-0.270803.390202.37000HepG2HNRNPA2B1
EIF2AK4ENSG00000128829.71229.544601855.98730-0.594106.453405.53710HepG2HNRNPLL
EIF2AK4ENSG00000128829.72743.535701935.643500.5031011.8202010.64100HepG2HNRNPU
EIF2AK4ENSG00000128829.74075.066302890.117700.495605.319604.41040HepG2IGF2BP2
EIF2AK4ENSG00000128829.71647.720601977.19860-0.263004.230403.34290HepG2ILF2
EIF2AK4ENSG00000128829.71469.082501864.08240-0.343602.883702.05900HepG2MSI2
EIF2AK4ENSG00000128829.72225.534303155.37660-0.503702.995902.33740HepG2NCBP2
EIF2AK4ENSG00000128829.71163.713101645.62730-0.499904.917203.93020HepG2NSUN2
EIF2AK4ENSG00000128829.71182.980301892.10150-0.677604.165203.06050HepG2PABPC1
EIF2AK4ENSG00000128829.72166.635602824.28840-0.382407.308306.39870HepG2PCBP1
EIF2AK4ENSG00000128829.72346.483001447.731900.696608.710707.36360HepG2PRPF8
EIF2AK4ENSG00000128829.71975.100002873.73250-0.5410013.4383012.41330HepG2PTBP1
EIF2AK4ENSG00000128829.72113.536702763.07520-0.386609.096408.15290HepG2PUM1
EIF2AK4ENSG00000128829.72184.179002761.02800-0.338103.909703.11500HepG2PUM2
EIF2AK4ENSG00000128829.72372.469302824.73120-0.251702.290001.67090HepG2RBM25
EIF2AK4ENSG00000128829.72638.004603198.26030-0.277804.250403.60560HepG2NELFE
EIF2AK4ENSG00000128829.73055.535802402.776600.346603.159902.43730HepG2RPLP0
EIF2AK4ENSG00000128829.73070.072002001.778200.616904.411803.22560HepG2RPS10
EIF2AK4ENSG00000128829.73058.113402162.670600.499705.662304.54200HepG2RPS2
EIF2AK4ENSG00000128829.72961.181702078.377800.510603.682402.75950HepG2RPS3A
EIF2AK4ENSG00000128829.71853.360701432.110000.371902.542901.78550HepG2RRP9
EIF2AK4ENSG00000128829.71411.205201723.78610-0.288703.995803.24530HepG2SAFB2
EIF2AK4ENSG00000128829.72562.937602992.08170-0.223402.985102.37470HepG2SF3B4
EIF2AK4ENSG00000128829.72178.679902495.81270-0.196102.465501.79750HepG2SLBP
EIF2AK4ENSG00000128829.74340.642103647.489700.250904.310203.72490HepG2SRFBP1
EIF2AK4ENSG00000128829.74684.393303123.022200.5848019.3345018.32280HepG2SRSF1
EIF2AK4ENSG00000128829.71888.798002540.69030-0.427809.109608.21150HepG2SRSF9
EIF2AK4ENSG00000128829.73688.855203166.857700.220003.181502.64070HepG2SSB
EIF2AK4ENSG00000128829.72590.206601868.202300.471304.062203.26650HepG2SUPV3L1
EIF2AK4ENSG00000128829.74009.255002806.918300.5142010.885309.88990HepG2TAF15
EIF2AK4ENSG00000128829.72071.899502579.36210-0.316103.392102.47070HepG2TIA1
EIF2AK4ENSG00000128829.71189.449801897.64860-0.6739012.3650011.29720HepG2TRIM56
EIF2AK4ENSG00000128829.7784.216401103.61170-0.492903.975803.06580HepG2UCHL5
EIF2AK4ENSG00000128829.71210.767602003.91200-0.726905.989104.14610HepG2XRCC5
EIF2AK4ENSG00000128829.72540.123001944.051200.385708.106307.05830HepG2ZC3H8
EIF2AK4ENSG00000128829.71829.564602242.34420-0.293504.225403.58800HepG2ZNF622
EIF2AK4ENSG00000128829.71252.911902022.63040-0.690909.512608.52840K562AQR
EIF2AK4ENSG00000128829.71758.332001402.188100.326403.081101.97740K562DDX3X
EIF2AK4ENSG00000128829.72522.053202056.373300.294404.105303.33250K562DDX47
EIF2AK4ENSG00000128829.7810.875801187.01220-0.549803.807902.27200K562EEF2
EIF2AK4ENSG00000128829.71248.877401540.14840-0.302403.624402.22020K562EIF2S1
EIF2AK4ENSG00000128829.71020.502201523.46100-0.578109.996108.93020K562FXR1
EIF2AK4ENSG00000128829.7766.561801149.83430-0.585005.233403.25640K562GEMIN5
EIF2AK4ENSG00000128829.71520.789302077.50040-0.450005.234904.19010K562GRSF1
EIF2AK4ENSG00000128829.7740.853001295.87400-0.8067010.053908.97270K562HNRNPC
EIF2AK4ENSG00000128829.7768.894001160.35480-0.593705.127503.81540K562HNRNPM
EIF2AK4ENSG00000128829.71409.413001702.39940-0.272502.229901.40980K562MATR3
EIF2AK4ENSG00000128829.71190.093101517.82530-0.350902.954801.94510K562PCBP2
EIF2AK4ENSG00000128829.7998.808901500.33350-0.587005.631204.43910K562PCBP3
EIF2AK4ENSG00000128829.7782.913801150.82020-0.555706.864105.61060K562POLR2G
EIF2AK4ENSG00000128829.7833.001501116.05680-0.422003.187302.38180K562PRPF8
EIF2AK4ENSG00000128829.7794.113501161.83370-0.549004.095402.96500K562PSIP1
EIF2AK4ENSG00000128829.71223.741701657.19720-0.437404.834603.97950K562PTBP1
EIF2AK4ENSG00000128829.72781.089103230.62840-0.216202.523201.60500K562PUM2
EIF2AK4ENSG00000128829.71177.691701589.42870-0.432504.141603.08940K562RBM22
EIF2AK4ENSG00000128829.71348.326701801.93340-0.418406.004304.83990K562SF3A3
EIF2AK4ENSG00000128829.71010.666501556.68070-0.623209.777508.12130K562SF3B4
EIF2AK4ENSG00000128829.71539.253401846.12210-0.262303.549602.50610K562SRPK2
EIF2AK4ENSG00000128829.71138.290201439.48390-0.338704.016502.58340K562SRSF7
EIF2AK4ENSG00000128829.7999.753301644.29490-0.717808.095806.80740K562TARDBP
EIF2AK4ENSG00000128829.71605.379701948.56890-0.279503.844702.33760K562TROVE2
EIF2AK4ENSG00000128829.71073.026501451.17380-0.435503.161801.98070K562U2AF1
EIF2AK4ENSG00000128829.71012.223201498.68040-0.566208.038506.65830K562U2AF2
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for EIF2AK4 is not available on ENCORE database.





9 RBPs can affect the splicing of EIF2AK4 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEEIF2AK4ENSG00000128829.7chr15+4032436940324438403225594032265740326526403266450.086000.002340.07920includedHepG2MATR3
SEEIF2AK4ENSG00000128829.7chr15+4032436940324438403225594032265740324957403250020.091000.000000.00016includedHepG2SRSF1
SEEIF2AK4ENSG00000128829.7chr15+403087024030887340303481403035474030930840309443-0.088000.001050.03192excludedHepG2SF3A3
SEEIF2AK4ENSG00000128829.7chr15+4032436940324438403225594032265740326526403266450.129000.000010.00159includedHepG2IGF2BP3
SEEIF2AK4ENSG00000128829.7chr15+403087024030887340303481403035474030930840309443-0.079000.001070.05064excludedHepG2PCBP1
SEEIF2AK4ENSG00000128829.7chr15+4032436940324438403225594032265740324957403250020.091000.000880.04208includedHepG2SF3B4
A3SSEIF2AK4ENSG00000128829.7chr15+4024779640247935402478204024793540246104402461850.065000.007530.04135longerK562AQR
A3SSEIF2AK4ENSG00000128829.7chr15+4024606940246185402461044024618540241316402414690.068000.002790.01729longerK562AQR
MXEEIF2AK4ENSG00000128829.7chr15+40231705402318184023558340235686402263464022654040241316402414690.148000.008110.045491includedHepG2U2AF2
MXEEIF2AK4ENSG00000128829.7chr15+4032436940324438403249574032500240322559403226574032652640326645-0.170000.000460.038052includedHepG2SRSF7

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for EIF2AK4 is not avaliable on ENCORE database.