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EIF2AK4 RNA-Seq in K562



EIF2AK4      CRISPR in K562      Control:

General Information
RBPEIF2AK4
Cell_LineK562
MethodCRISPR
Exp_NameEIF2AK4-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1EIF2AK4-BGKcLV42-31
Rep2EIF2AK4-BGKcLV42-32
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR24.4
Rep2_qPCR20.5
Rep1_WB70.3
Rep2_WB74.9
Antibody Cat#65981S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1539




Experiment Information (Status: Sequenced)
BGKcLV42-31BGKcLV42-32
idx00
TRCN#_or_BGC#BGC#0001134BGC#0001134
shRNA_or_gRNA_sequenceCGCGCCGTAAATGGCCTCCACGCGCCGTAAATGGCCTCCA
PAMGGGGGG
NameEIF2AK4_94EIF2AK4_94
Sample_IDBGKcLV42-31BGKcLV42-32
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameEIF2AK4EIF2AK4
qPCR_result24.420.5
Ave_qPCR22.4
RT-qPCR_primer-Faatggtaaacatcgggcaaa
RT-qPCR_primer-Rgctgatcaggactccccata
protein_conc34893548
WB_result70.374.9
Ave_WB72.6
WB_DONE_date5/14/24
MW220kd
IP
antibody_Cat#65981Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID57915792




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
EIF2AK4Product_ID: 65981S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: EIF2AK4-human		 CST_65981S_1_EIF2AK4.png<br>Caption: IP-WB analysis of 65981S whole cell lysate using the EIF2AK4 specific antibody, 65981S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the EIF2AK4-specific antibody, 65981S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 EIF2AK4-K562-CRISPR-65981S.png<br>Caption: Western blot following CRISPR against EIF2AK4 in K562 whole cell lysate using EIF2AK4 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against EIF2AK4. EIF2AK4 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-31BGKcLV42-32
Sample_IDBGKcLV42-31BGKcLV42-32
Sample Name
Sample NameEIF2AK4-BGKcLV42-31EIF2AK4-BGKcLV42-32
RBPEIF2AK4EIF2AK4
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1307213073




Library-Prep Information
BGKcLV42-31BGKcLV42-32
Sample #2930
Sample NameEIF2AK4-BGKcLV42-31EIF2AK4-BGKcLV42-32
Sample_Name_Alias
Index Well PositionE04F04
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp295291
Peak_Molarity101.00102.00
libSampleQC_DNA_WellDNA_Library_set73/set1/E4.pngDNA_Library_set73/set1/F4.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/E4.pngRNA_Library_set73/set1/F4.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-31BGKcLV42-32
RBPEIF2AK4EIF2AK4
Batch_IDBGKcLV42BGKcLV42
WB_result70.30074.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID42984299




Sequencing Information
BGKcLV42-31BGKcLV42-32
Sample_IDBGKcLV42-31BGKcLV42-32
Sample NameEIF2AK4-BGKcLV42-31EIF2AK4-BGKcLV42-32
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads41,463,69739,605,846
total_aligned_reads37,403,94137,173,492
unique_aligned_reads34,514,77634,272,966
percent_uniqueAligned0.832410.86535
correlation_replicates0.9984980.998498
spikein_reads2,5543,468
percent_spikeins0.000060.00009
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31653166




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database