PRPF8

General Information

Gene Name : PRPF8
Gene ID : ENSG00000174231.16
Essential Gene : Yes

Functions

Splicing regulation
Spliceosome

Localization(RBPImage: PRPF8)

Nuclei
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: PRPF8)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all PRPF8 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	PRPF8	ENSG00000174231.16	121.53	154.19	137.86	ENCSR000AEF
HepG2	PRPF8	ENSG00000174231.16	219.76	146.84	183.30	ENCSR985KAT
K562	PRPF8	ENSG00000174231.16	150.31	150.57	150.44	ENCSR000AEO

Assays

5 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 278	ENCSR534YOI	PRPF8	K562	Released	ENCAB207WIC	ENCSR096SZE
eCLIP	eCLIP: 291	ENCSR121NVA	PRPF8	HepG2	Released	ENCAB207WIC	ENCSR565KBR
knockdown followed by RNA-seq	PRPF8-BGKLV19-K562	ENCSR137HKS	PRPF8	K562	Released		ENCSR661HEL	shRNA	KD% = ,
knockdown followed by RNA-seq	PRPF8-BGHLV17-HepG2	ENCSR998MZP	PRPF8	HepG2	Released		ENCSR856ZRV	shRNA	KD% = 84.1,90.1
knockdown followed by RNA-seq	PRPF8-BGKcLV08-K562	ENCSR320IEK	PRPF8	K562	Released		ENCSR015KGT	CRISPR	KD% = 62.4,66.2

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
PRPF8	278	K562	ENCSR534YOI	ENCSR096SZE	ENCAB207WIC	A303-921A	1	Released	ENCODE3
PRPF8	291	HepG2	ENCSR121NVA	ENCSR565KBR	ENCAB207WIC	A303-921A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
PRPF8	ENCAB207WIC	A303-921A	1		Released, ENCODE3	Released, ENCODE3	BGHLV17-23, Released, ENCODE3	BGKcLV08-51, Released, ENCODE4
PRPF8	ENCAB100KQS	RN094PW	1		Released, ENCODE3	Released, ENCODE3		BGKcLV08-51, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
PRPF8	K562	shRNA	ENCSR137HKS	ENCSR661HEL	PRPF8_BGKLV19_43B,PRPF8_BGKLV19_44B	NT_BGKLV19_1B,NT_BGKLV19_2B	,	Released	ENCODE3
PRPF8	HepG2	shRNA			BGHLV16-43,BGHLV16-44	BGHLV16-1,BGHLV16-2		NotSatisfied	ENCODE3
PRPF8	HepG2	shRNA	ENCSR998MZP	ENCSR856ZRV	PRPF8_BGHLV17_23,PRPF8_BGHLV17_24	NT_BGHLV17_63,NT_BGHLV17_64	84.1,90.1	Released	ENCODE3
PRPF8	K562	CRISPR	ENCSR320IEK	ENCSR015KGT	PRPF8-BGKcLV08-51,PRPF8-BGKcLV08-52	NT-BGKcLV08-1,NT-BGKcLV08-2	62.4,66.2	Released	ENCODE4
PRPF8	K562	CRISPR			BGKcLV08-53,BGKcLV08-54	BGKcLV08-1,BGKcLV08-2	49.9,36.4	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

PRPF8: Click to RBP Image Database

Nuclei

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
PRPF8	Product_ID: A303-921A Lot_ID: 1 Source: Bethyl Labs Target Name: PRPF8-human
PRPF8	Product_ID: RN094PW Lot_ID: 1 Source: MBLI Target Name: PRPF8-human

RNA Targets

PRPF8 protein binds to 30,893 RNA regions.

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Proteins bound to RNA

PRPF8 RNA is bound by 45 proteins.

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PRPF8 protein has 30,893 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
PRPF8	chr2	74756211	74756238	-	3.61237	80.68846	HepG2	AUP1	intron-Distal,intron-Proximal
PRPF8	chr17	74382051	74382114	+	3.38849	66.62943	HepG2	SPHK1	CDS,intron-Proximal
PRPF8	chr17	4905404	4905499	+	5.24776	41.24400	HepG2	KIF1C	CDS,intron-Proximal
PRPF8	chr12	112846143	112846247	-	4.87739	27.30359	HepG2	RPL6	CDS,intron-Distal,intron-Proximal
PRPF8	chr2	21235985	21236014	-	4.86415	25.52126	HepG2	APOB	intron-Distal,intron-Proximal
PRPF8	chr16	89967179	89967261	+	3.26985	24.30909	HepG2	TCF25	CDS,intron-Proximal
PRPF8	chr12	46766277	46766289	-	3.41978	24.27383	HepG2	SLC38A2	intron-Distal,intron-Proximal
PRPF8	chr11	85721982	85722069	-	4.26155	24.06871	K562	PICALM	intron-Distal,intron-Proximal
PRPF8	chr2	63265924	63265992	+	3.54750	23.99835	HepG2	EHBP1	CDS,intron-Proximal
PRPF8	chr7	66313627	66313688	+	4.56129	20.92431	HepG2	RP11-458F8.1	intron-Distal,intron-Proximal

Showing 1 to 10 of 100 entries

45 proteins are bound to PRPF8 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	1577825	1577899	-	CDS,intron-Distal,intron-Proximal	8.40363	112.10741	K562	LIN28B
chr17	1580916	1580989	-	CDS,intron-Distal,intron-Proximal	5.65256	68.46544	K562	FXR2
chr17	1564326	1564384	-	CDS,intron-Distal	6.15926	45.73158	K562	FXR2
chr17	1565212	1565288	-	CDS,intron-Distal	5.79100	39.36628	K562	FMR1
chr17	1580920	1580989	-	CDS,intron-Distal,intron-Proximal	3.99312	39.06128	K562	FMR1
chr17	1577150	1577186	-	CDS,intron-Distal	5.50076	38.87279	K562	FXR2
chr17	1553953	1554023	-	3'-UTR,CDS,intron-Distal	4.84057	36.86487	HepG2	SUB1
chr17	1564938	1564973	-	CDS,intron-Distal	5.88075	35.25372	K562	FXR2
chr17	1564586	1564645	-	CDS,intron-Distal,intron-Proximal	5.95215	34.55798	K562	FXR2
chr17	1554942	1555067	-	CDS,intron-Distal,intron-Proximal	5.03057	33.77920	HepG2	G3BP1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 545 entries

The PRPF8 protein binds to its own RNA.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
PRPF8	chr17	1563087	1563169	-	3.91266	25.62419	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1564249	1564311	-	4.01722	21.74143	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1579468	1579531	-	5.40399	18.53372	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1577682	1577754	-	4.31084	16.28235	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1577642	1577731	-	6.21828	15.81095	K562	PRPF8	intron-Distal,intron-Proximal
PRPF8	chr17	1580823	1580888	-	3.60477	14.15837	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1583978	1584042	-	4.08662	13.02279	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1582544	1582587	-	4.33961	12.61233	HepG2	PRPF8	CDS,intron-Distal,intron-Proximal
PRPF8	chr17	1586731	1586798	-	4.24728	11.71462	K562	PRPF8	intron-Distal,intron-Proximal
PRPF8	chr17	1564238	1564296	-	3.30130	11.38887	K562	PRPF8	CDS,intron-Distal,intron-Proximal

Showing 1 to 10 of 23 entries

Diffrential Expression

10,494 differentially expressed genes after Knockdown/Knockout PRPF8 protein.

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102 RBPs can affect the levels of PRPF8.

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10,494 differentially expressed genes after Knockdown/Knockout PRPF8 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
ANLN	ENSG00000011426.6	534.91390	1865.75080	-1.80240	43.70490	41.16390	HepG2	PRPF8
BRCA1	ENSG00000012048.15	216.02370	760.99110	-1.81670	30.02980	27.82700	HepG2	PRPF8
NCAPD2	ENSG00000010292.8	1194.64690	2813.76090	-1.23590	26.60860	24.51120	HepG2	PRPF8
MGST1	ENSG00000008394.8	623.43380	1564.50510	-1.32740	24.43540	22.41270	HepG2	PRPF8
MVP	ENSG00000013364.14	1501.49170	560.54030	1.42140	24.40970	22.39090	HepG2	PRPF8
SLC7A2	ENSG00000003989.12	1154.55210	2699.47960	-1.22530	24.06640	22.06670	HepG2	PRPF8
SKAP2	ENSG00000005020.8	1327.25640	508.31760	1.38450	23.92430	21.93200	HepG2	PRPF8
CX3CL1	ENSG00000006210.6	2961.39390	1276.96550	1.21350	22.13170	20.18130	HepG2	PRPF8
CROT	ENSG00000005469.7	565.97710	191.85980	1.56060	18.92400	17.08710	HepG2	PRPF8
E2F2	ENSG00000007968.6	43.65510	207.67540	-2.25010	17.43370	15.65990	HepG2	PRPF8
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

102 RBPs can affect the gene expression of PRPF8 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PRPF8	ENSG00000174231.12	1861.26500	6258.48090	-1.74950	57.01740	54.09740	HepG2	PRPF8
PRPF8	ENSG00000174231.12	7210.17120	12988.65090	-0.84910	35.78030	34.32790	HepG2	RBM22
PRPF8	ENSG00000174231.12	6708.51530	10934.57670	-0.70480	25.16450	23.62670	HepG2	CELF1
PRPF8	ENSG00000174231.12	9675.84740	17046.90490	-0.81700	22.32700	20.07020	K562	GRSF1
PRPF8	ENSG00000174231.12	5437.65030	9951.47420	-0.87190	18.44610	16.49470	K562	PRPF8
PRPF8	ENSG00000174231.12	19111.80550	13627.61140	0.48780	17.79520	16.47640	K562	AGGF1
PRPF8	ENSG00000174231.12	7015.85420	9499.28230	-0.43720	18.06310	16.37550	HepG2	ILF2
PRPF8	ENSG00000174231.12	12924.09900	9648.23590	0.42160	16.78210	15.44160	HepG2	CKAP4
PRPF8	ENSG00000174231.12	5887.65150	8290.77750	-0.49380	16.53580	15.38990	HepG2	SRSF9
PRPF8	ENSG00000174231.12	5610.68900	9795.98910	-0.80400	17.10980	14.92150	K562	EIF4A3
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 114 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

3,616 alternative splicing events after Knockdown/Knockout PRPF8 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	IVNS1ABP	ENSG00000116679.11	chr1	-	185275616	185275757	185274667	185274775	185276145	185276271	0.24800	0.00000	included	K562	PRPF8
SE	HCFC1	ENSG00000172534.9	chrX	-	153226488	153226624	153225685	153225865	153226992	153227099	0.06100	0.00031	included	K562	PRPF8
SE	MVD	ENSG00000167508.6	chr16	-	88728431	88728575	88725057	88725128	88729418	88729569	0.07300	0.00000	included	K562	PRPF8
SE	EIF4H	ENSG00000106682.10	chr7	+	73604576	73604636	73604151	73604248	73609070	73609208	0.13100	0.00005	included	K562	PRPF8
SE	SREK1	ENSG00000153914.11	chr5	+	65451892	65454760	65449290	65449424	65455046	65455162	0.30000	0.00000	included	K562	PRPF8
SE	IST1	ENSG00000182149.16	chr16	+	71956254	71956277	71955245	71955356	71956376	71956583	0.13800	0.00000	included	K562	PRPF8
SE	SEC24C	ENSG00000176986.10	chr10	+	75506387	75506474	75504148	75504195	75506562	75506762	0.09700	0.00000	included	K562	PRPF8
SE	AC013394.2	ENSG00000272888.1	chr15	+	93426814	93427008	93426244	93426416	93428744	93428814	0.41200	0.00000	included	K562	PRPF8
SE	NBR1	ENSG00000188554.9	chr17	+	41323090	41323319	41322510	41322960	41327807	41327918	0.43900	0.00007	included	K562	PRPF8
SE	CPED1	ENSG00000106034.13	chr7	+	120884895	120885063	120884255	120884392	120906280	120906501	0.07900	0.00002	included	K562	PRPF8

Showing 1 to 10 of 100 entries

6 RBPs can affect the splicing of PRPF8 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	PRPF8	ENSG00000174231.12	chr17	-	1577039	1577186	1576650	1576861	1577735	1577974	-0.13700	0.00049	excluded	HepG2	PRPF8
SE	PRPF8	ENSG00000174231.12	chr17	-	1564286	1564456	1563991	1564121	1564564	1564700	-0.06300	0.00000	excluded	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1562650	1562867	1562650	1562842	1563134	1563295	0.05200	0.00003	longer	HepG2	SF3A3
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1562650	1562867	1562650	1562842	1563134	1563295	0.05100	0.00010	longer	K562	AKAP8L
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1585422	1585611	1585422	1585587	1586826	1587071	0.07200	0.00000	longer	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1579221	1579423	1579221	1579348	1579500	1579664	0.06300	0.00000	longer	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1584222	1584405	1584222	1584348	1584771	1584984	0.08500	0.00000	longer	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1577937	1577988	1577937	1577974	1578445	1578673	0.10100	0.00000	longer	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1576650	1576894	1576650	1576861	1577039	1577224	0.06400	0.00000	longer	K562	AQR
A3SS	PRPF8	ENSG00000174231.12	chr17	-	1562650	1562867	1562650	1562842	1563134	1563295	0.26300	0.00000	longer	K562	AQR

Showing 1 to 10 of 22 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The PRPF8 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	PRPF8	ENSG00000174231.12	chr17	-	1577039	1577186			1576650	1576861	1577735	1577974	-0.13700	0.00000	0.00049	excluded	HepG2	PRPF8

Showing 1 to 1 of 1 entries