DROSHA

General Information

Gene Name : DROSHA
Gene ID : ENSG00000113360.16
Essential Gene : Yes

Functions

microRNA processing

Localization(RBPImage: DROSHA)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Cytoskeleton
Microtubule
Actin
Cytosolic

Domains (AlphaFold: DROSHA)

Nuclease
dRBM

Antibody Validation

View validation images for all DROSHA antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	DROSHA	ENSG00000113360.16	29.60	22.97	26.29	ENCSR000AEF
HepG2	DROSHA	ENSG00000113360.16	42.84	43.49	43.17	ENCSR985KAT
K562	DROSHA	ENSG00000113360.16	43.14	48.18	45.66	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 481	ENCSR653HQC	DROSHA	K562	Released	ENCAB936LAQ	ENCSR828NSY
eCLIP	eCLIP: 575	ENCSR834YLD	DROSHA	HepG2	Released	ENCAB936LAQ	ENCSR785VKF
knockdown followed by RNA-seq	DROSHA-BGKLV28-K562	ENCSR624XHG	DROSHA	K562	Released		ENCSR164MUK	shRNA	KD% = 68.6,71.4
knockdown followed by RNA-seq	DROSHA-BGHcLV05-HepG2	ENCSR991XFO	DROSHA	HepG2	Released		ENCSR481WJH	CRISPR	KD% = 60.3,89.3

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
DROSHA	481	K562	ENCSR653HQC	ENCSR828NSY	ENCAB936LAQ	A301-886A	1	Released	ENCODE3
DROSHA	575	HepG2	ENCSR834YLD	ENCSR785VKF	ENCAB936LAQ	A301-886A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
DROSHA	ENCAB953TNR	3364	3	Released, ENCODE3	Released, ENCODE3	BGHcLV05-13, Released, ENCODE3
DROSHA	ENCAB936LAQ	A301-886A	1	Released, ENCODE3	Released, ENCODE3	NotReviewed, ENCODE3	BGKLV28-45, Released, ENCODE3
DROSHA		3364s	5	Released, ENCODE3	Submitted, ENCORE2	NotReviewed, ENCODE3	BGKcLV42-25, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
DROSHA	K562	shRNA	ENCSR624XHG	ENCSR164MUK	RNASEN50_BGKLV28-45,RNASEN50_BGKLV28-46	non-target_BGKLV28-1,non-target_BGKLV28-2	68.6,71.4	Released	ENCODE3
DROSHA	HepG2	shRNA			BGHLV26-45,BGHLV26-46	BGHLV26-1,BGHLV26-2	,no clear band	NotSatisfied	ENCODE3
DROSHA	HepG2	shRNA			BGHLV26-47,BGHLV26-48	BGHLV26-1,BGHLV26-2		NotSatisfied	ENCODE3
DROSHA	HepG2	shRNA			BGHLV26-49,BGHLV26-50	BGHLV26-1,BGHLV26-2		NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-9,BGKcLV04-10	BGKcLV04-1,BGKcLV04-2	too light band,	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-9-D12,BGKcLV04-10-D12	BGKcLV04-1,BGKcLV04-2	no clear band,	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-10-D12,BGKcLV04-9-D12	BGKcLV04-1,BGKcLV04-2	,33.5	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-9-D20,BGKcLV04-10-D20	BGKcLV04-1,BGKcLV04-2	no clear band,	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-10-D20,BGKcLV04-9-D20	BGKcLV04-1,BGKcLV04-2	,31.0	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-9-D30,BGKcLV04-10-D30	BGKcLV04-1,BGKcLV04-2		NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			BGKcLV04-10-D30,BGKcLV42-25	BGKcLV04-1,BGKcLV04-2	,72.2	NotSatisfied	ENCODE3
DROSHA	K562	CRISPR			DROSHA-BGKcLV42-25,DROSHA-BGKcLV42-26	NT-BGKcLV42-1,NT-BGKcLV42-2	72.2,65.0	Submitting	ENCORE2
DROSHA	HepG2	CRISPR	ENCSR991XFO	ENCSR481WJH	DROSHA_BGHcLV05-13,DROSHA_BGHcLV05-14	NT_BGHcLV05-1,NT_BGHcLV05-2	60.3,89.3	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

DROSHA: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Cytoskeleton

Microtubule

Actin

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DROSHA	Product_ID: A301-886A Lot_ID: 1 Source: Bethyl Labs Target Name: DROSHA-human
DROSHA	Product_ID: 3364 Lot_ID: 3 Source: Cell Signaling Technology Target Name: DROSHA-human
DROSHA	Product_ID: 3364s Lot_ID: 5 Source: Cell Signaling Technology Target Name: DROSHA-human

RNA Targets

DROSHA protein binds to 5,849 RNA regions.

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Proteins bound to RNA

DROSHA RNA is bound by 35 proteins.

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DROSHA protein has 5,849 RNA targets:

Only display 100 entries as example. Please click "View All" button to see all 5,849 entries.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
DROSHA	chr21	46282952	46282955	-	8.03023	400.00000	HepG2	PTTG1IP	intron-Distal
DROSHA	chr2	10476082	10476113	+	7.34373	400.00000	K562	HPCAL1	intron-Distal
DROSHA	chr7	100173030	100173039	-	8.34296	213.35604	K562	LRCH4	intron-Distal
DROSHA	chr9	96938272	96938288	+	9.55786	118.76117	K562	RP11-2B6.3	intron-Distal
DROSHA	chr12	62997416	62997487	+	9.46942	112.74084	K562	RP11-631N16.2	intron-Distal
DROSHA	chr13	92003941	92003991	+	6.41374	97.68022	HepG2	MIR17HG	intron-Distal
DROSHA	chr13	92003304	92003325	+	6.30420	85.85508	HepG2	MIR17HG	intron-Distal
DROSHA	chr9	139564801	139564831	+	7.36968	70.02792	K562	EGFL7	intron-Proximal
DROSHA	chr18	55990440	55990455	+	7.93160	64.70729	HepG2	NEDD4L	intron-Distal,intron-Proximal
DROSHA	chr13	92004429	92004449	+	6.30554	62.61344	HepG2	MIR17HG	intron-Distal

Showing 1 to 10 of 100 entries

35 proteins are bound to DROSHA RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr5	31526641	31526749	-	CDS,intron-Distal	6.96729	35.22399	K562	HNRNPK
chr5	31526654	31526697	-	CDS,intron-Distal	6.44710	31.35395	HepG2	PCBP2
chr5	31526639	31526755	-	CDS,intron-Distal	5.34907	29.53355	HepG2	DDX59
chr5	31526547	31526641	-	CDS,intron-Distal,intron-Proximal	4.89951	20.84757	HepG2	HNRNPK
chr5	31526554	31526619	-	CDS,intron-Distal,intron-Proximal	4.41856	20.06311	HepG2	PCBP2
chr5	31401453	31401598	-	3'-UTR,CDS,intron-Distal,intron-Proximal	5.43399	19.54402	K562	UCHL5
chr5	31526646	31526711	-	CDS,intron-Distal	5.39434	19.03216	HepG2	HNRNPK
chr5	31515092	31515218	-	CDS,intron-Distal,intron-Proximal	3.43406	17.51411	K562	ZNF622
chr5	31503315	31503404	-	intron-Distal	5.93950	16.92594	HepG2	SUGP2
chr5	31526697	31526753	-	CDS,intron-Distal	5.09440	15.91391	HepG2	PCBP2
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 149 entries

No autoregularoty interactions between protein DROSHA and its RNA.

Diffrential Expression

80 differentially expressed genes after Knockdown/Knockout DROSHA protein.

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88 RBPs can affect the levels of DROSHA.

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80 differentially expressed genes after Knockdown/Knockout DROSHA protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
AC005280.1	ENSG00000170468.5	60.62820	0.00000	99999.99990	14.48710	10.39010	K562	DROSHA
C1orf132	ENSG00000203709.5	171.36400	21.17260	3.01670	14.48570	10.39010	K562	DROSHA
C1orf132	ENSG00000203709.5	212.53110	31.22980	2.76660	12.65060	8.32500	HepG2	DROSHA
XAGE1D	ENSG00000204376.7	693.17970	228.02210	1.60400	11.24320	7.32370	K562	DROSHA
RP11-430N14.4	ENSG00000271875.1	1369.20090	193.55690	2.82240	10.77340	6.97880	K562	DROSHA
DROSHA	ENSG00000113360.12	1979.84850	4924.70530	-1.31460	10.24670	6.62830	K562	DROSHA
SLCO2B1	ENSG00000137491.10	439.68210	133.29940	1.72170	10.30510	6.62830	K562	DROSHA
YES1	ENSG00000176105.9	3555.15070	8676.12950	-1.28710	9.66800	6.11650	K562	DROSHA
STON1-GTF2A1L	ENSG00000068781.16	0.00000	33.43260	-99999.99999	8.94110	5.44760	K562	DROSHA
ENTPD3	ENSG00000168032.4	173.94890	43.73830	1.99160	8.24490	4.83360	K562	DROSHA
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 80 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

88 RBPs can affect the gene expression of DROSHA :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
DROSHA	ENSG00000113360.12	1040.49630	3127.84990	-1.58790	34.82330	32.76720	HepG2	CPSF6
DROSHA	ENSG00000113360.12	1391.74640	3406.97770	-1.29160	33.06280	31.39380	HepG2	BOP1
DROSHA	ENSG00000113360.12	1439.33410	2357.92730	-0.71210	26.08010	24.83140	HepG2	ZNF622
DROSHA	ENSG00000113360.12	1943.81190	1085.03580	0.84100	25.07780	23.96580	HepG2	SRSF1
DROSHA	ENSG00000113360.12	1325.76830	2143.94320	-0.69340	19.80010	18.26230	HepG2	UPF1
DROSHA	ENSG00000113360.12	1902.38670	3383.82930	-0.83080	13.50030	12.25110	HepG2	HNRNPLL
DROSHA	ENSG00000113360.12	3203.75650	5032.28740	-0.65140	12.54920	10.91930	K562	GRSF1
DROSHA	ENSG00000113360.12	2432.35390	3978.25030	-0.70980	11.90800	10.81480	K562	AQR
DROSHA	ENSG00000113360.12	2839.24370	5195.59560	-0.87180	12.41750	10.74760	K562	CPSF6
DROSHA	ENSG00000113360.12	825.90180	1305.13690	-0.66020	11.58920	10.23060	HepG2	DNAJC21
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

1,616 alternative splicing events after Knockdown/Knockout DROSHA protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	EPB41L2	ENSG00000079819.12	chr6	-	131199243	131199390	131190702	131191266	131206235	131206408	0.09600	0.00107	included	K562	DROSHA
SE	FAM177A1	ENSG00000151327.8	chr14	+	35546355	35546422	35522405	35522657	35548132	35548230	0.10900	0.00001	included	K562	DROSHA
SE	DPM1	ENSG00000000419.8	chr20	-	49557401	49557470	49552684	49552799	49558567	49558663	0.09100	0.00147	included	K562	DROSHA
SE	GPBP1	ENSG00000062194.11	chr5	+	56532939	56532999	56531792	56531859	56542126	56542311	0.18200	0.00010	included	K562	DROSHA
SE	ALDOA	ENSG00000149925.12	chr16	+	30077196	30077248	30075744	30075826	30078554	30078687	0.13900	0.00139	included	K562	DROSHA
SE	XPO6	ENSG00000169180.7	chr16	-	28188540	28188653	28187218	28187416	28192261	28192352	0.14200	0.00008	included	K562	DROSHA
SE	PI4KAP2	ENSG00000183506.12	chr22	-	21830932	21830993	21830280	21830449	21832078	21832204	0.57400	0.00000	included	K562	DROSHA
SE	NQO2	ENSG00000124588.15	chr6	+	3004728	3004885	3000222	3000319	3006701	3006793	0.05200	0.00003	included	K562	DROSHA
SE	RAB1A	ENSG00000138069.12	chr2	-	65318116	65318212	65313988	65315824	65325104	65325200	0.11500	0.00015	included	K562	DROSHA
SE	WNK1	ENSG00000060237.12	chr12	+	989886	990165	987377	987527	990857	990955	0.05700	0.00214	included	K562	DROSHA

Showing 1 to 10 of 100 entries

20 RBPs can affect the splicing of DROSHA :

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Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	DROSHA	ENSG00000113360.12	chr5	-	31489088	31489188	31486597	31486669	31493313	31493400	0.07500	0.00009	included	K562	SRSF1
SE	DROSHA	ENSG00000113360.12	chr5	-	31424533	31424578	31422893	31423051	31429581	31429652	-0.12100	0.00012	excluded	K562	SF3B4
SE	DROSHA	ENSG00000113360.12	chr5	-	31409356	31409439	31409162	31409266	31410852	31411053	-0.13600	0.00000	excluded	K562	AQR
SE	DROSHA	ENSG00000113360.12	chr5	-	31521229	31521322	31515560	31515671	31526185	31526397	-0.34300	0.00008	excluded	K562	U2AF1
SE	DROSHA	ENSG00000113360.12	chr5	-	31521229	31521322	31515560	31515671	31526185	31526397	-0.42300	0.00000	excluded	K562	U2AF2
SE	DROSHA	ENSG00000113360.12	chr5	-	31521229	31521322	31515560	31515671	31526185	31526397	0.21500	0.00001	included	K562	HNRNPU
SE	DROSHA	ENSG00000113360.12	chr5	-	31521229	31521322	31515560	31515671	31526185	31526397	-0.22000	0.00029	excluded	K562	RBM15
SE	DROSHA	ENSG00000113360.12	chr5	-	31531556	31531632	31530904	31531031	31532096	31532216	0.26200	0.00010	included	K562	HNRNPC
SE	DROSHA	ENSG00000113360.12	chr5	-	31467248	31467273	31466288	31466388	31468045	31468113	0.44300	0.00000	included	K562	HNRNPC
A3SS	DROSHA	ENSG00000113360.12	chr5	-	31421378	31421569	31421378	31421484	31422893	31423051	0.07900	0.00000	longer	K562	AQR

Showing 1 to 10 of 55 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

DROSHA

General Information

Functions

Localization(RBPImage: DROSHA)

Domains (AlphaFold: DROSHA)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

DROSHA protein has 5,849 RNA targets:

35 proteins are bound to DROSHA RNA:

No autoregularoty interactions between protein DROSHA and its RNA.

Diffrential Expression

80 differentially expressed genes after Knockdown/Knockout DROSHA protein :

88 RBPs can affect the gene expression of DROSHA :

1,616 alternative splicing events after Knockdown/Knockout DROSHA protein :

20 RBPs can affect the splicing of DROSHA :

No autoregularoty alternative splicing events between protein DROSHA and its RNA.