TUFM

General Information

Gene Name : TUFM
Gene ID : ENSG00000178952.10
Essential Gene : Yes

Functions

mitochondrial RNA regulation

Localization(RBPImage: TUFM)

Cytoplasm
Compartment/Organelle
Mitochondria

Domains (AlphaFold: TUFM)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

GeneTex: GTX101763

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	TUFM	ENSG00000178952.10	58.22	117.14	87.68	ENCSR000AEF
HepG2	TUFM	ENSG00000178952.10	311.46	211.07	261.26	ENCSR985KAT
K562	TUFM	ENSG00000178952.10	270.54	238.33	254.44	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	TUFM-BGKLV25-K562	ENCSR602AWR	TUFM	K562	Released		ENCSR620PUP	shRNA	KD% = 71.1,65.4
knockdown followed by RNA-seq	TUFM-BGHLV23-HepG2	ENCSR459EMR	TUFM	HepG2	Released		ENCSR042QTH	shRNA	KD% = 77.0,72.2

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
TUFM	ENCAB531CVZ	GTX101763	39694				BGHLV23-53, Released, ENCODE3	BGKLV25-59, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
TUFM	K562	shRNA	ENCSR602AWR	ENCSR620PUP	TUFM-BGKLV25-59,TUFM-BGKLV25-60	NT_BGKLV25-1,NT_BGKLV25-2	71.1,65.4	Released	ENCODE3
TUFM	HepG2	shRNA	ENCSR459EMR	ENCSR042QTH	TUFM-BGHLV23-53,TUFM-BGHLV23-54	non-target-BGHLV23-1,non-target-BGHLV23-2	77.0,72.2	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

TUFM: Click to RBP Image Database

Cytoplasm

Compartment/Organelle

Mitochondria

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
TUFM	Product_ID: GTX101763 Lot_ID: 39694 Source: GeneTex Target Name: TUFM-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for TUFM is not avaliable on ENCORE database.

Proteins bound to RNA

TUFM RNA is bound by 31 proteins.

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eCLIP assay for TUFM is not avaliable on ENCORE database.

31 proteins are bound to TUFM RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr16	28857584	28857641	-	5'-UTR,CDS,intron-Distal	5.50809	86.06439	HepG2	DDX3X
chr16	28854225	28854245	-	3'-UTR,intron-Distal	5.12892	85.32369	K562	PABPC4
chr16	28854177	28854214	-	3'-UTR,intron-Distal	5.17345	79.50888	K562	PABPC4
chr16	28854360	28854386	-	CDS,intron-Distal	4.32933	78.24265	K562	YBX3
chr16	28854353	28854360	-	CDS,intron-Distal	4.34022	68.26760	K562	YBX3
chr16	28857541	28857584	-	CDS,intron-Distal	4.82250	65.96706	HepG2	DDX3X
chr16	28854214	28854225	-	3'-UTR,intron-Distal	5.30522	62.90731	K562	PABPC4
chr16	28857320	28857363	-	CDS,intron-Distal	4.80310	60.80752	HepG2	DDX3X
chr16	28854386	28854393	-	CDS,intron-Distal	4.05929	57.64170	K562	YBX3
chr16	28854245	28854269	-	3'-UTR,intron-Distal	4.92406	56.74814	K562	PABPC4
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 163 entries

eCLIP assay for TUFM is not avaliable on ENCORE database.

Diffrential Expression

4,572 differentially expressed genes after Knockdown/Knockout TUFM protein.

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122 RBPs can affect the levels of TUFM.

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4,572 differentially expressed genes after Knockdown/Knockout TUFM protein :

Only display 100 entries as example. Please click "View All" button to see all 4,572 entries.

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Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CX3CL1	ENSG00000006210.6	4281.03220	1846.98290	1.21270	40.11130	36.96050	HepG2	TUFM
MGST1	ENSG00000008394.8	1387.38370	2596.52940	-0.90420	20.89090	18.34850	HepG2	TUFM
ACSM3	ENSG00000005187.7	1789.24910	1016.21260	0.81610	15.04120	12.83310	HepG2	TUFM
MLXIPL	ENSG00000009950.11	5785.53980	2835.31390	1.02880	14.43230	12.26330	HepG2	TUFM
ARSD	ENSG00000006756.11	652.29160	356.79800	0.87030	11.14890	9.19590	HepG2	TUFM
DBNDD1	ENSG00000003249.9	1290.60310	771.03760	0.74310	11.02920	9.09210	HepG2	TUFM
ANLN	ENSG00000011426.6	2251.74830	3388.98920	-0.58980	10.36580	8.47320	HepG2	TUFM
AK2	ENSG00000004455.12	2818.70680	4212.86880	-0.57980	10.29070	8.40780	HepG2	TUFM
MAN2B2	ENSG00000013288.4	1192.07270	596.34220	0.99920	9.80380	7.97040	HepG2	TUFM
LUC7L	ENSG00000007392.12	963.98250	1527.28340	-0.66390	9.51970	7.71130	HepG2	TUFM
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

122 RBPs can affect the gene expression of TUFM :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
TUFM	ENSG00000178952.4	1380.39360	5544.73900	-2.00600	64.13640	60.60030	HepG2	TUFM
TUFM	ENSG00000178952.4	2645.71190	8808.26590	-1.73520	52.52700	48.73300	K562	TUFM
TUFM	ENSG00000178952.4	2696.87590	6549.92610	-1.28020	47.23900	44.90600	K562	HNRNPC
TUFM	ENSG00000178952.4	6825.09040	10157.52450	-0.57360	39.03040	36.79270	K562	NSUN2
TUFM	ENSG00000178952.4	5257.95170	8053.45240	-0.61510	27.84360	26.68640	HepG2	SRFBP1
TUFM	ENSG00000178952.4	4152.46070	7130.46490	-0.78000	27.86490	26.56140	HepG2	RBM22
TUFM	ENSG00000178952.4	7043.53110	10770.26420	-0.61270	25.30610	23.39940	K562	UPF1
TUFM	ENSG00000178952.4	585.91650	2314.64210	-1.98200	25.33410	22.44780	K562	G3BP2
TUFM	ENSG00000178952.4	6089.28840	9142.23730	-0.58630	23.31730	21.30620	K562	MTPAP
TUFM	ENSG00000178952.4	2936.21470	4287.55320	-0.54620	19.88200	18.37230	HepG2	ZC3H8
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 151 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for TUFM is not available on ENCORE database.

3 RBPs can affect the splicing of TUFM :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A3SS	TUFM	ENSG00000178952.4	chr16	-	28855739	28855936			28855739	28855872	28856018	28856183	0.06100	0.00000	0.00000	longer	K562	AQR
A5SS	TUFM	ENSG00000178952.4	chr16	-	28855550	28855655			28855634	28855655	28855272	28855422	-0.28500	0.00000	0.00000	shorter	K562	SUPV3L1
MXE	TUFM	ENSG00000178952.4	chr16	-	28855634	28855655	28855739	28855896	28855272	28855422	28856018	28856183	0.56500	0.00001	0.00011	1included	K562	AQR
MXE	TUFM	ENSG00000178952.4	chr16	-	28855634	28855655	28855739	28855910	28855272	28855422	28856018	28856183	0.52100	0.00008	0.00096	1included	K562	AQR
RI	TUFM	ENSG00000178952.4	chr16	-	28855270	28855653			28855270	28855422	28855634	28855653	0.16200	0.00050	0.07467	included	K562	HNRNPA1

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

TUFM

General Information

Functions

Localization(RBPImage: TUFM)

Domains (AlphaFold: TUFM)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for TUFM is not avaliable on ENCORE database.

31 proteins are bound to TUFM RNA:

eCLIP assay for TUFM is not avaliable on ENCORE database.

Diffrential Expression

4,572 differentially expressed genes after Knockdown/Knockout TUFM protein :

122 RBPs can affect the gene expression of TUFM :

The Knockdown/knockout RNA-Seq assay for TUFM is not available on ENCORE database.

3 RBPs can affect the splicing of TUFM :

The Knockdown/knockout RNA-Seq assay for TUFM is not avaliable on ENCORE database.