PUS1

General Information

Gene Name : PUS1
Gene ID : ENSG00000177192.13
Essential Gene : No

Functions

RNA modification
tRNA regulation

Localization(RBPImage: PUS1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Compartment/Organelle
Mitochondria
Mitotic-Apparatus (Spindle; Centrosomes)

Domains (AlphaFold: PUS1)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A301-651A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	PUS1	ENSG00000177192.13	4.79	11.76	8.28	ENCSR000AEF
HepG2	PUS1	ENSG00000177192.13	45.54	14.87	30.20	ENCSR985KAT
K562	PUS1	ENSG00000177192.13	28.77	20.38	24.57	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 668	ENCSR291XPT	PUS1	K562	Released	ENCAB680DCY	ENCSR869TOB
knockdown followed by RNA-seq	PUS1-BGKLV32-K562	ENCSR618IQH	PUS1	K562	Released		ENCSR143COQ	shRNA	KD% = 72.4,70.4
knockdown followed by RNA-seq	PUS1-BGHLV31-HepG2	ENCSR296ERI	PUS1	HepG2	Released		ENCSR538QOG	shRNA	KD% = 83.3,79.6

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
PUS1	668	K562	ENCSR291XPT	ENCSR869TOB	ENCAB680DCY	A301-651A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
PUS1	ENCAB680DCY	A301-651A	1			Released, ENCODE3	BGHLV31-51, Released, ENCODE3	BGKLV32-51, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
PUS1	K562	shRNA	ENCSR618IQH	ENCSR143COQ	PUS1_BGKLV32-51,PUS1_BGKLV32-52	NT_BGKLV32-1,NT_BGKLV32-2	72.4,70.4	Released	ENCODE3
PUS1	HepG2	shRNA			BGHLV18-45,BGHLV18-46	BGHLV18-1,BGHLV18-2		NotSatisfied	ENCODE3
PUS1	HepG2	shRNA			BGHLV27-45,BGHLV27-46	BGHLV27-1,BGHLV27-2		NotSatisfied	ENCODE3
PUS1	HepG2	shRNA	ENCSR296ERI	ENCSR538QOG	PUS1_BGHLV31-51,PUS1_BGHLV31-52	NT-BGHLV31-1,NT-BGHLV31-2	83.3,79.6	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

PUS1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Compartment/Organelle

Mitochondria

Mitotic-Apparatus (Spindle; Centrosomes)

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
PUS1	Product_ID: A301-651A Lot_ID: 1 Source: Bethyl Labs Target Name: PUS1-human

RNA Targets

PUS1 protein binds to 9 RNA regions.

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Proteins bound to RNA

PUS1 RNA is bound by 28 proteins.

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PUS1 protein has 9 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
PUS1	chr7	139025499	139025531	+	4.89011	400.00000	K562	C7orf55-LUC7L2, C7orf55	intron-Distal
PUS1	chr7	139025494	139025499	+	4.24778	400.00000	K562	C7orf55-LUC7L2, C7orf55	intron-Distal
PUS1	chr7	139025474	139025494	+	3.75170	400.00000	K562	C7orf55-LUC7L2, C7orf55	intron-Distal
PUS1	chrM	15083	15194	+	3.41370	74.77650	K562	MT-CYB	CDS
PUS1	chrM	9935	9946	+	3.86904	30.79474	K562	MT-CO3	CDS
PUS1	chrM	15194	15240	+	3.22921	21.62404	K562	MT-CYB	CDS
PUS1	chr19	1383572	1383639	+	6.84134	14.06678	K562	NDUFS7	5'-UTR,intron-Proximal
PUS1	chr22	49921133	49921237	-	4.21767	7.88058	K562	C22orf34	intron-Distal
PUS1	chr22	49921293	49921363	-	3.79199	7.07700	K562	C22orf34	intron-Distal

Showing 1 to 9 of 9 entries

28 proteins are bound to PUS1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr12	132414214	132414302	+	5'-UTR,CDS,intron-Distal,intron-Proximal	5.44336	21.70648	HepG2	PRPF4
chr12	132413975	132414059	+	5'-UTR,intron-Distal,intron-Proximal	5.01309	20.73954	HepG2	PRPF4
chr12	132414540	132414633	+	CDS,intron-Distal	5.11636	20.42599	HepG2	PPIG
chr12	132414189	132414292	+	5'-UTR,CDS,intron-Distal,intron-Proximal	3.62204	14.08896	HepG2	FAM120A
chr12	132413908	132413975	+	5'-UTR,intron-Distal,intron-Proximal	4.31614	13.27593	HepG2	PRPF4
chr12	132417759	132417835	+	intron-Distal	4.14149	13.07323	HepG2	HNRNPC
chr12	132414507	132414618	+	CDS,intron-Distal	5.36670	13.02724	HepG2	SRSF1
chr12	132414026	132414177	+	5'-UTR,intron-Distal,intron-Proximal	5.75214	12.79104	HepG2	GTF2F1
chr12	132414059	132414115	+	5'-UTR,intron-Distal,intron-Proximal	6.02632	12.62377	HepG2	PRPF4
chr12	132414231	132414297	+	5'-UTR,CDS,intron-Distal,intron-Proximal	3.91238	12.53034	K562	GTF2F1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 70 entries

No autoregularoty interactions between protein PUS1 and its RNA.

Diffrential Expression

16,063 differentially expressed genes after Knockdown/Knockout PUS1 protein.

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90 RBPs can affect the levels of PUS1.

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16,063 differentially expressed genes after Knockdown/Knockout PUS1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SLC7A2	ENSG00000003989.12	2171.02320	7458.30520	-1.78050	184.55770	181.38210	HepG2	PUS1
CRLF1	ENSG00000006016.6	1396.30730	5076.48410	-1.86220	149.95310	146.95360	HepG2	PUS1
RHOBTB2	ENSG00000008853.12	1254.87040	330.47210	1.92480	81.45340	79.11910	HepG2	PUS1
JHDM1D	ENSG00000006459.6	1051.50550	2460.61190	-1.22660	61.00860	58.90080	HepG2	PUS1
SPAG9	ENSG00000008294.16	2547.75470	4605.92060	-0.85430	41.77250	39.94430	HepG2	PUS1
FKBP4	ENSG00000004478.5	841.12320	1786.54510	-1.08680	41.10280	39.28540	HepG2	PUS1
GDE1	ENSG00000006007.7	3112.93280	1693.34290	0.87830	34.66860	32.94410	HepG2	PUS1
CYP51A1	ENSG00000001630.11	9307.80860	5581.52350	0.73770	33.55040	31.84930	HepG2	PUS1
CROT	ENSG00000005469.7	645.59160	235.07150	1.45740	31.84640	30.17770	HepG2	PUS1
MARK4	ENSG00000007047.10	419.91520	941.98700	-1.16560	30.32450	28.68600	HepG2	PUS1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

90 RBPs can affect the gene expression of PUS1 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PUS1	ENSG00000177192.9	131.30340	399.81380	-1.60640	25.50800	23.98620	HepG2	PUS1
PUS1	ENSG00000177192.9	128.44610	442.05810	-1.78310	21.79730	20.29950	K562	PUS1
PUS1	ENSG00000177192.9	627.41710	236.05120	1.41020	22.25670	19.68090	K562	MAGOH
PUS1	ENSG00000177192.9	804.94370	352.56200	1.19090	19.96260	18.45060	K562	FXR1
PUS1	ENSG00000177192.9	1196.52000	662.31730	0.85310	19.06690	17.50570	K562	DDX47
PUS1	ENSG00000177192.9	441.13030	184.88370	1.25450	15.29390	13.36750	K562	EIF3G
PUS1	ENSG00000177192.9	485.68890	227.52650	1.09390	11.69010	10.07050	K562	TARDBP
PUS1	ENSG00000177192.9	580.61530	349.73100	0.73120	9.98020	8.92300	HepG2	SUCLG1
PUS1	ENSG00000177192.9	253.37390	107.45320	1.23750	10.56400	8.84000	K562	SF3B4
PUS1	ENSG00000177192.9	531.60160	330.64070	0.68500	9.19730	8.24900	HepG2	PUM1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 97 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

5,475 alternative splicing events after Knockdown/Knockout PUS1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	NSUN3	ENSG00000178694.5	chr3	+	93782655	93782823	93781854	93781982	93783280	93783362	0.51700	0.00000	included	K562	PUS1
SE	RP11-611E13.2	ENSG00000257815.1	chr12	-	70636845	70636984	70636576	70636673	70637091	70637129	0.22100	0.00005	included	K562	PUS1
SE	SPRED2	ENSG00000198369.5	chr2	-	65571569	65571728	65561762	65561907	65571852	65571920	0.39100	0.00000	included	K562	PUS1
SE	EHMT1	ENSG00000181090.13	chr9	+	140676559	140676655	140674086	140674169	140676742	140676849	0.12200	0.00009	included	K562	PUS1
SE	ANKRD52	ENSG00000139645.9	chr12	-	56637283	56637464	56637021	56637176	56637568	56637660	0.05600	0.00006	included	K562	PUS1
SE	WAC	ENSG00000095787.17	chr10	+	28900183	28900410	28899627	28899750	28900702	28900851	0.14100	0.00000	included	K562	PUS1
SE	LINC00969	ENSG00000242086.4	chr3	+	195401690	195401737	195400688	195400858	195404597	195404938	0.22500	0.00007	included	K562	PUS1
SE	SREBF1	ENSG00000072310.12	chr17	-	17720861	17720905	17720569	17720771	17721009	17721230	0.28500	0.00011	included	K562	PUS1
SE	EIF2B5	ENSG00000145191.7	chr3	+	183856634	183856727	183855953	183856034	183857867	183857945	0.12800	0.00000	included	K562	PUS1
SE	RB1CC1	ENSG00000023287.8	chr8	-	53600154	53600359	53599412	53599527	53626634	53627026	0.10300	0.00011	included	K562	PUS1

Showing 1 to 10 of 100 entries

9 RBPs can affect the splicing of PUS1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.57600	0.00000	0.00000	excluded	K562	HNRNPC
MXE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820	132425836	132426528	132416719	132416857	132428083	132428406	-0.33500	0.00000	0.00006	2included	K562	EFTUD2
MXE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820	132425836	132426528	132416719	132416857	132428083	132428406	-0.55000	0.00000	0.00000	2included	K562	HNRNPC
MXE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820	132425836	132426528	132416719	132416857	132428083	132428406	-0.36900	0.00000	0.00001	2included	K562	SUPV3L1
MXE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820	132425836	132426528	132416719	132416857	132428083	132428406	-0.27600	0.00001	0.00050	2included	K562	PRPF8
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.18100	0.00002	0.00183	excluded	K562	PRPF8
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.17900	0.00002	0.00034	excluded	K562	AQR
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.20200	0.00002	0.00214	excluded	K562	EFTUD2
MXE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820	132425836	132426528	132416719	132416857	132428083	132428406	-0.16000	0.00003	0.00035	2included	K562	AQR
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.28000	0.00020	0.00440	excluded	K562	PUS1

Showing 1 to 10 of 13 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The PUS1 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
SE	PUS1	ENSG00000177192.9	chr12	+	132423717	132423820			132416719	132416857	132425836	132426528	-0.28000	0.00020	0.00440	excluded	K562	PUS1

Showing 1 to 1 of 1 entries