METTL3

General Information

Gene Name : METTL3
Gene ID : ENSG00000165819.11
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: METTL3)

Not Available

Antibody Validation

View validation images for all METTL3 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	METTL3	ENSG00000165819.11	43.83	48.59	46.21	ENCSR000AEF
HepG2	METTL3	ENSG00000165819.11	89.82	100.63	95.22	ENCSR985KAT
K562	METTL3	ENSG00000165819.11	49.37	50.32	49.84	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	METTL3-BGKcLV23-K562	ENCSR335KFN	METTL3	K562	Released		ENCSR877JEM	CRISPR	KD% = 61.3,66.3
knockdown followed by RNA-seq	METTL3-BGHcLV22-HepG2	ENCSR884REL	METTL3	HepG2	Released		ENCSR704VPL	CRISPR	KD% = 71.3,87.0

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
METTL3	ENCAB373NTV	A301-568A	1				BGHcLV22-31, Released, ENCODE4	BGKcLV23-53, Released, ENCODE4
METTL3		86132S	1			Submitted, ENCORE2	BGHcLV22-31, Released, ENCODE4	BGKcLV23-53, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
METTL3	K562	CRISPR			BGKcLV21-29,BGKcLV21-30	BGKcLV21-1,BGKcLV21-2	0.0,0.0	NotSatisfied	ENCODE4
METTL3	K562	CRISPR	ENCSR335KFN	ENCSR877JEM	METTL3-BGKcLV23-53,METTL3-BGKcLV23-54	NT-BGKcLV23-1,NT-BGKcLV23-2	61.3,66.3	Released	ENCODE4
METTL3	HepG2	CRISPR	ENCSR884REL	ENCSR704VPL	METTL3-BGHcLV22-31,METTL3-BGHcLV22-32	NT-BGHcLV22-1,NT-BGHcLV22-2	71.3,87.0	Released	ENCODE4
METTL3	HepG2	CRISPR			BGHcLV22-33,BGHcLV22-34	BGHcLV22-1,BGHcLV22-2	45.9,58.0	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
METTL3	Product_ID: 86132S Lot_ID: 1 Source: Cell Signaling Technology Target Name: METTL3-human
METTL3	Product_ID: A301-568A Lot_ID: 1 Source: Bethyl Labs Target Name: METTL3-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for METTL3 is not avaliable on ENCORE database.

Proteins bound to RNA

METTL3 RNA is bound by 18 proteins.

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eCLIP assay for METTL3 is not avaliable on ENCORE database.

18 proteins are bound to METTL3 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr14	21967252	21967327	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.49911	20.94432	K562	SF3B4
chr14	21967436	21967536	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.45976	16.66938	HepG2	BCLAF1
chr14	21967221	21967295	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.14651	15.90466	HepG2	U2AF2
chr14	21966440	21966514	-	3'-UTR,CDS,intron-Distal	4.41202	15.61961	HepG2	GRWD1
chr14	21979271	21979399	-	5'-UTR,CDS,intron-Distal	4.24413	14.18457	HepG2	GRWD1
chr14	21967619	21967690	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.56546	13.68298	HepG2	BCLAF1
chr14	21969831	21969903	-	3'-UTR,CDS,intron-Distal,intron-Proximal	5.16280	13.47152	HepG2	PRPF8
chr14	21979391	21979450	-	5'-UTR,intron-Distal,intron-Proximal	4.03240	13.22798	HepG2	NCBP2
chr14	21967602	21967715	-	3'-UTR,CDS,intron-Distal,intron-Proximal	3.64170	13.18299	HepG2	PPIG
chr14	21966658	21966704	-	intron-Distal	5.74503	12.93612	HepG2	QKI
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 58 entries

eCLIP assay for METTL3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for METTL3 is not available on ENCORE database.
118 RBPs can affect the levels of METTL3.

CanvasJS.com

The Knockdown/knockout RNA-Seq assay for METTL3 is not available on ENCORE database.

118 RBPs can affect the gene expression of METTL3 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
METTL3	ENSG00000165819.7	1097.08940	2329.51730	-1.08630	35.86110	34.40730	HepG2	RBM22
METTL3	ENSG00000165819.7	1214.01970	2427.49220	-0.99970	35.77940	33.80710	HepG2	AUH
METTL3	ENSG00000165819.7	1461.69590	2787.13240	-0.93110	34.47050	32.77640	HepG2	ETF1
METTL3	ENSG00000165819.7	1037.48300	2417.84250	-1.22060	32.22380	30.47220	HepG2	PUF60
METTL3	ENSG00000165819.7	1332.69410	2387.26830	-0.84100	26.97050	25.29740	HepG2	SRSF5
METTL3	ENSG00000165819.7	455.34300	1247.85990	-1.45440	24.31220	22.62520	HepG2	GPKOW
METTL3	ENSG00000165819.7	831.43570	1473.57830	-0.82560	22.70930	21.34600	HepG2	ACO1
METTL3	ENSG00000165819.7	497.27320	1196.65250	-1.26690	20.82070	18.91300	HepG2	PRPF8
METTL3	ENSG00000165819.7	839.21470	1382.78780	-0.72050	19.05250	17.93400	HepG2	ZNF622
METTL3	ENSG00000165819.7	902.49200	1473.25960	-0.70700	18.45140	17.06440	HepG2	CKAP4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 141 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for METTL3 is not available on ENCORE database.

28 RBPs can affect the splicing of METTL3 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	METTL3	ENSG00000165819.7	chr14	-	21968636	21968824	21967907	21967946	21969054	21969271	-0.16500	0.00000	0.00000	excluded	HepG2	U2AF1
SE	METTL3	ENSG00000165819.7	chr14	-	21969054	21969271	21967907	21967946	21971315	21971720	-0.33400	0.00000	0.00007	excluded	K562	PUS1
A3SS	METTL3	ENSG00000165819.7	chr14	-	21969869	21970045	21969869	21969966	21971315	21971720	-0.11400	0.00000	0.00651	shorter	K562	EIF4G2
A3SS	METTL3	ENSG00000165819.7	chr14	-	21966305	21966549	21966305	21966513	21967168	21967299	0.09600	0.00000	0.00000	longer	K562	AQR
A3SS	METTL3	ENSG00000165819.7	chr14	-	21967168	21967295	21967168	21967281	21967449	21967515	0.19400	0.00000	0.00005	longer	HepG2	HNRNPC
RI	METTL3	ENSG00000165819.7	chr14	-	21971315	21971972	21971315	21971720	21971806	21971972	0.26200	0.00000	0.00007	included	HepG2	SF3A3
RI	METTL3	ENSG00000165819.7	chr14	-	21971315	21971972	21971315	21971720	21971806	21971972	0.23600	0.00000	0.00002	included	HepG2	U2AF2
RI	METTL3	ENSG00000165819.7	chr14	-	21971315	21971972	21971315	21971720	21971806	21971972	0.06900	0.00000	0.00002	included	HepG2	RBM22
RI	METTL3	ENSG00000165819.7	chr14	-	21967168	21967744	21967168	21967281	21967635	21967744	-0.21600	0.00000	0.00001	excluded	K562	TARDBP
SE	METTL3	ENSG00000165819.7	chr14	-	21978973	21979122	21971806	21972024	21979265	21979484	0.20400	0.00001	0.00080	included	K562	AGGF1

Showing 1 to 10 of 48 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

METTL3

General Information

Functions

Localization

Domains (AlphaFold: METTL3)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for METTL3 is not avaliable on ENCORE database.

18 proteins are bound to METTL3 RNA:

eCLIP assay for METTL3 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for METTL3 is not available on ENCORE database.

118 RBPs can affect the gene expression of METTL3 :

The Knockdown/knockout RNA-Seq assay for METTL3 is not available on ENCORE database.

28 RBPs can affect the splicing of METTL3 :

The Knockdown/knockout RNA-Seq assay for METTL3 is not avaliable on ENCORE database.