DDX20

General Information

Gene Name       : DDX20
Gene ID             : ENSG00000064703.11
Essential Gene : Yes

Functions

Localization(RBPImage: DDX20)

Domains (AlphaFold: DDX20)


Antibody Validation

 View validation images for all DDX20 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DDX20ENSG00000064703.1120.4116.8318.62ENCSR000AEF
HepG2DDX20ENSG00000064703.116.8612.209.53ENCSR985KAT
K562DDX20ENSG00000064703.1117.6917.5717.63ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DDX20-BGKcLV08-K562ENCSR867XALDDX20K562ReleasedENCSR015KGT   CRISPRKD% = 56.0,56.0
knockdown followed by RNA-seq DDX20-BGHcLV10-HepG2ENCSR429OURDDX20HepG2ReleasedENCSR775ZWO   CRISPRKD% = 64.2,54.6






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
DDX20390HepG2ENCSR722KBGENCSR240ZFUENCAB711GMEA300-651A1RevokedENCODE3


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DDX20ENCAB711GMEA300-651A1Released, ENCODE3Released, ENCODE3BGHcLV10-19, Released, ENCODE4BGKcLV08-17, Released, ENCODE4
DDX20ENCAB398QAOA300-650A1Released, ENCODE3Released, ENCODE4BGHcLV10-19, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DDX20K562shRNABGKLV08-11,BGKLV08-12BGKLV08-1,BGKLV08-227.5,45.9NotSatisfiedENCODE3
DDX20K562shRNABGKLV13-47,BGKLV13-48BGKLV13-1,BGKLV13-2NotSatisfiedENCODE3
DDX20K562shRNABGKLV28-9,BGKLV28-10BGKLV28-1,BGKLV28-221.3,48.0NotSatisfiedENCODE3
DDX20K562shRNABGKLV32-19,BGKLV32-20BGKLV32-1,BGKLV32-2NotSatisfiedENCODE3
DDX20HepG2shRNABGHLV30-23,BGHLV30-24BGHLV30-1,BGHLV30-241.8,34.2NotSatisfiedENCODE3
DDX20HepG2shRNABGHLV31-27,BGHLV31-28BGHLV31-1,BGHLV31-2NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-5,BGKcLV04-6BGKcLV04-1,BGKcLV04-20.0,0.0NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-5-D12,BGKcLV04-6-D12BGKcLV04-1,BGKcLV04-20.0,0.0NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-6-D12,BGKcLV04-5-D20BGKcLV04-1,BGKcLV04-20.0,18.9NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-5-D20,BGKcLV04-6-D20BGKcLV04-1,BGKcLV04-218.9,43.5NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-6-D20,BGKcLV04-5-D30BGKcLV04-1,BGKcLV04-243.5,0.0NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-5-D30,BGKcLV04-6-D30BGKcLV04-1,BGKcLV04-20.0,0.0NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV04-6-D30,BGKcLV08-15BGKcLV04-1,BGKcLV04-20.0,50.0NotSatisfiedENCODE3
DDX20K562CRISPRBGKcLV08-15,BGKcLV08-16BGKcLV08-1,BGKcLV08-250.0,61.0NotSatisfiedENCODE4
DDX20K562CRISPRENCSR867XALENCSR015KGTDDX20-BGKcLV08-17,DDX20-BGKcLV08-18NT-BGKcLV08-1,NT-BGKcLV08-256.0,56.0ReleasedENCODE4
DDX20HepG2CRISPRBGHcLV05-9,BGHcLV05-10BGHcLV05-1,BGHcLV05-231.6,32.3NotSatisfiedENCODE4
DDX20HepG2CRISPRBGHcLV05-9-D13,BGHcLV05-10-D13BGHcLV05-1,BGHcLV05-20.0,0.0NotSatisfiedENCODE4
DDX20HepG2CRISPRBGHcLV05-10-D13,BGHcLV10-17BGHcLV05-1,BGHcLV05-20.0,54.5NotSatisfiedENCODE4
DDX20HepG2CRISPRBGHcLV10-17,BGHcLV10-18BGHcLV10-1,BGHcLV10-254.5,73.0NotSatisfiedENCODE4
DDX20HepG2CRISPRENCSR429OURENCSR775ZWODDX20-BGHcLV10-19,DDX20-BGHcLV10-20NT-BGHcLV10-1,NT-BGHcLV10-264.2,54.6ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    DDX20: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Nucleolus
  • Cytoplasm
  • Compartment/Organelle
  • P bodies
  • Unknown Cyto Foci
  • Cytosolic
  • Nuclear release mitosis












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    DDX20Product_ID: A300-651A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: DDX20-human    		 HepG2_Bethyl_A300-651A_1_DDX20.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX20 antibody (lanes under 'DDX20').
    		 DDX20-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DDX20 in HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
    		 K562_Bethyl_A300-651A_1_DDX20.png<br>Caption: IP-WB characterization of DDX20 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal DDX20 Antibody. Lane 4 is the immunoprecipitation of DDX20 antibody in K562 lysate. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
    		 DDX20-K562-CRISPR.png<br>Caption: Western blot following CRISPR against DDX20 in K562 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
    DDX20Product_ID: A300-650A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: DDX20-human    		 Bethyl_A300-650A_1_DDX20.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX20 specific antibody, A300-650A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX20-specific antibody, A300-650A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








    RNA Targets

    • eCLIP assay for DDX20 is not avaliable on ENCORE database.



    Proteins bound to RNA
    • DDX20 RNA is bound by 4 proteins.  







    eCLIP assay for DDX20 is not avaliable on ENCORE database.





    4 proteins are bound to DDX20 RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr1112305501112305583+3'-UTR,CDS,intron-Proximal3.372203.91614K562BUD13
    chr1112304956112305002+3'-UTR,5'-UTR,CDS,intron-Proximal4.527314.13847K562AQR
    chr1112305515112305564+3'-UTR,CDS,intron-Proximal4.577074.13847K562AQR
    chr1112305300112305387+3'-UTR,5'-UTR,CDS5.082666.51488K562LIN28B
    chr1112303868112303938+3'-UTR,5'-UTR,CDS,intron-Proximal3.013723.94281K562PRPF8
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for DDX20 is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for DDX20 is not available on ENCORE database.

    • 70 RBPs can affect the levels of DDX20.  








    The Knockdown/knockout RNA-Seq assay for DDX20 is not available on ENCORE database.





    70 RBPs can affect the gene expression of DDX20 :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    DDX20ENSG00000064703.7542.27370322.418700.750004.945704.26140HepG2AKAP1
    DDX20ENSG00000064703.7383.13890480.72520-0.327302.543401.82740HepG2DAZAP1
    DDX20ENSG00000064703.7172.81450265.31500-0.618502.600802.00150HepG2DDX6
    DDX20ENSG00000064703.7190.39970305.32640-0.681304.727603.82960HepG2DNAJC21
    DDX20ENSG00000064703.7234.01870302.63980-0.371002.079801.49900HepG2DNAJC2
    DDX20ENSG00000064703.7135.32280255.04720-0.914405.029004.24210HepG2EIF2S1
    DDX20ENSG00000064703.7438.86640274.430500.677203.428902.66560HepG2EIF4B
    DDX20ENSG00000064703.7230.80950454.28170-0.976909.234107.78340HepG2EIF4G2
    DDX20ENSG00000064703.7722.41730581.345100.313302.489301.91470HepG2ETF1
    DDX20ENSG00000064703.7595.59130396.914300.585403.498102.63970HepG2FIP1L1
    DDX20ENSG00000064703.7418.22220284.388500.556303.114002.37940HepG2FXR1
    DDX20ENSG00000064703.7308.47320512.26540-0.731707.163506.07990HepG2HNRNPF
    DDX20ENSG00000064703.7302.83800437.91920-0.532104.805003.93430HepG2HNRNPL
    DDX20ENSG00000064703.7332.22730447.20420-0.428802.158801.59630HepG2HNRNPLL
    DDX20ENSG00000064703.7419.09950273.883200.613604.498803.31840HepG2HNRNPM
    DDX20ENSG00000064703.7284.98900504.44100-0.823808.120206.93930HepG2IGF2BP1
    DDX20ENSG00000064703.7476.61730284.487800.744405.263404.36470HepG2MAGOH
    DDX20ENSG00000064703.7577.27220286.078501.012708.859407.78310HepG2MATR3
    DDX20ENSG00000064703.7429.61590292.816000.553003.037802.37550HepG2NCBP2
    DDX20ENSG00000064703.7375.88610264.232700.508403.505502.84000HepG2PCBP1
    DDX20ENSG00000064703.7334.30600455.02210-0.444802.767302.17050HepG2PUF60
    DDX20ENSG00000064703.7379.04580482.66700-0.348702.101301.51220HepG2PUM2
    DDX20ENSG00000064703.7524.06240304.206200.784603.880903.04920HepG2RAVER1
    DDX20ENSG00000064703.7993.09450485.903401.0312020.6576019.51040HepG2RBM22
    DDX20ENSG00000064703.7458.66820299.221700.616105.001104.30940HepG2NELFE
    DDX20ENSG00000064703.7398.52900278.678800.516002.639002.03530HepG2RECQL
    DDX20ENSG00000064703.7397.84350551.74850-0.471802.958102.25520HepG2RPLP0
    DDX20ENSG00000064703.7296.98490219.611200.435301.916701.39660HepG2RPS19
    DDX20ENSG00000064703.7234.74850303.32550-0.369801.917201.36390HepG2SAFB2
    DDX20ENSG00000064703.71002.18450402.777901.3150016.2601014.55220HepG2SF3A3
    DDX20ENSG00000064703.7419.75850279.927700.584403.584702.92410HepG2SF3B4
    DDX20ENSG00000064703.7305.43690436.26160-0.514302.569401.89000HepG2SLBP
    DDX20ENSG00000064703.7442.29340353.725700.322302.088401.51780HepG2SMN1
    DDX20ENSG00000064703.7647.27650548.821500.237901.814101.38260HepG2SRFBP1
    DDX20ENSG00000064703.7681.37140292.181201.2215021.7188020.66160HepG2SRSF1
    DDX20ENSG00000064703.7273.98940503.26380-0.8772010.516209.30940HepG2SRSF7
    DDX20ENSG00000064703.7401.35390262.616300.611804.665803.96750HepG2TAF15
    DDX20ENSG00000064703.7322.85610435.92440-0.433202.339701.66540HepG2TARDBP
    DDX20ENSG00000064703.7436.18250249.326400.806803.344302.17390HepG2TBRG4
    DDX20ENSG00000064703.7243.07850330.95350-0.445202.384301.91390HepG2TIAL1
    DDX20ENSG00000064703.7249.00960333.92460-0.423302.703002.14360HepG2TRIM56
    DDX20ENSG00000064703.7638.91460339.561000.911908.796707.67150HepG2U2AF1
    DDX20ENSG00000064703.7275.01030357.20120-0.377302.251901.61120HepG2UPF1
    DDX20ENSG00000064703.7522.66150344.865600.599705.592704.78970HepG2UTP3
    DDX20ENSG00000064703.7484.69270334.569000.534702.620001.59220HepG2XRCC6
    DDX20ENSG00000064703.71005.81950658.601400.610802.952301.72660K562AARS
    DDX20ENSG00000064703.7513.56280681.58970-0.408403.638103.00370K562AGGF1
    DDX20ENSG00000064703.71809.087101428.492900.340703.097101.78140K562APOBEC3C
    DDX20ENSG00000064703.71776.980101351.949000.394301.837801.34040K562AQR
    DDX20ENSG00000064703.71978.010401305.351600.5995013.3516012.03180K562DDX47
    DDX20ENSG00000064703.7583.11100424.344900.458402.896501.51720K562DNAJC21
    DDX20ENSG00000064703.7860.80670654.837600.394502.912401.97680K562EFTUD2
    DDX20ENSG00000064703.7634.36860401.392600.660203.215502.12310K562EIF3A
    DDX20ENSG00000064703.7924.15710681.813300.438703.553202.65780K562EIF3G
    DDX20ENSG00000064703.7726.58720511.524500.506204.118003.11870K562EIF4A3
    DDX20ENSG00000064703.71010.995201284.26430-0.345202.685101.84940K562FIP1L1
    DDX20ENSG00000064703.7666.56790869.75940-0.383902.438201.58330K562FTO
    DDX20ENSG00000064703.71070.95530531.961101.0094021.3890019.81440K562FXR1
    DDX20ENSG00000064703.71036.13150582.125500.831707.517706.23310K562G3BP2
    DDX20ENSG00000064703.7716.64980962.77780-0.425903.481902.59460K562GPKOW
    DDX20ENSG00000064703.71081.30550744.958900.537404.357103.29820K562MAGOH
    DDX20ENSG00000064703.71066.19630843.194800.338402.602601.72500K562MBNL1
    DDX20ENSG00000064703.72197.615101770.609700.311602.995401.43650K562NOL12
    DDX20ENSG00000064703.7861.312301063.96320-0.304802.361701.66830K562NSUN2
    DDX20ENSG00000064703.7993.43950662.869800.583605.976504.52370K562PCBP2
    DDX20ENSG00000064703.72029.498901663.393300.286903.281101.72170K562PNPT1
    DDX20ENSG00000064703.71173.900201457.80780-0.312502.348701.59430K562PPIG
    DDX20ENSG00000064703.71302.67820637.229801.0315016.0062014.18210K562PRPF8
    DDX20ENSG00000064703.71118.00420723.757500.627206.095105.14140K562PTBP1
    DDX20ENSG00000064703.72471.101501878.438400.395505.542403.99110K562PUM1
    DDX20ENSG00000064703.7878.63540694.119200.340002.337301.56990K562RBM22
    DDX20ENSG00000064703.7966.04810649.163100.573404.622303.56480K562RPS19
    DDX20ENSG00000064703.7831.85320595.454300.482204.134502.80170K562SAFB
    DDX20ENSG00000064703.71107.03690709.498600.641708.303907.08820K562SRSF1
    DDX20ENSG00000064703.7700.94520897.27980-0.356303.520502.18860K562SRSF7
    DDX20ENSG00000064703.71166.62420670.269600.7994013.1576011.36900K562SYNCRIP
    DDX20ENSG00000064703.7818.65260564.603300.535903.337302.11220K562U2AF1
    DDX20ENSG00000064703.7715.16320583.002900.294702.015301.33010K562U2AF2
    DDX20ENSG00000064703.7760.88590965.32150-0.343302.135401.36800K562WDR3
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for DDX20 is not available on ENCORE database.





    1 RBPs can affect the splicing of DDX20 :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SEDDX20ENSG00000064703.7chr1+112303608112303747112303327112303470112303847112303906-0.063000.003350.03353excludedK562AQR
    SEDDX20ENSG00000064703.7chr1+112304876112304959112303847112303906112305298112305404-0.072000.000980.01203excludedK562AQR
    A3SSDDX20ENSG00000064703.7chr1+1123035951123037471123036081123037471123033271123034700.144000.000000.00001longerK562AQR

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for DDX20 is not avaliable on ENCORE database.