| HepG2 | 
Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX20 antibody (lanes under 'DDX20'). Method: immunoprecipitation Releated Sample: eCLIP:390 Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against DDX20 in HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGHcLV10-19 Status: Released Lab: Graveley Lab |
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| K562 | 
Caption: IP-WB characterization of DDX20 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal DDX20 Antibody. Lane 4 is the immunoprecipitation of DDX20 antibody in K562 lysate. This antibody passes preliminary validation and will be further pursued for primary and secondary validation. Method: immunoprecipitation Status: Released Lab: Yeo Lab | 
Caption: Western blot following CRISPR against DDX20 in K562 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow. Releated Sample: BGKcLV08-17 Status: Released Lab: Graveley Lab |
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