Antibodies of DDX20


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX20Product_ID: A300-651A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX20-human		 HepG2_Bethyl_A300-651A_1_DDX20.png<br>Caption: IP-Western Blot analysis of HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-DDX20 antibody (lanes under 'DDX20').
 DDX20-HEPG2-CRISPR.png<br>Caption: Western blot following CRISPR against DDX20 in HepG2 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 K562_Bethyl_A300-651A_1_DDX20.png<br>Caption: IP-WB characterization of DDX20 specific antibody in K562 cell line . Lane 1 is 2.5% of five million K562 whole cell lysate Input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using rabbit polyclonal DDX20 Antibody. Lane 4 is the immunoprecipitation of DDX20 antibody in K562 lysate. This antibody passes preliminary validation and will be further pursued for primary and secondary validation.
 DDX20-K562-CRISPR.png<br>Caption: Western blot following CRISPR against DDX20 in K562 whole cell lysate using DDX20 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DDX20.DDX20 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
DDX20Product_ID: A300-650A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DDX20-human		 Bethyl_A300-650A_1_DDX20.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX20 specific antibody, A300-650A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DDX20-specific antibody, A300-650A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.