CPSF2

General Information

Gene Name       : CPSF2
Gene ID             : ENSG00000165934.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CPSF2)


Antibody Validation

 View validation images for all CPSF2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CPSF2ENSG00000165934.1248.3535.3841.87ENCSR000AEF
HepG2CPSF2ENSG00000165934.1219.0631.0325.05ENCSR985KAT
K562CPSF2ENSG00000165934.1229.2737.5933.43ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CPSF2-BGKcLV28-K562ENCSR887XCLCPSF2K562ReleasedENCSR220DTX   CRISPRKD% = 70.5,67.6
knockdown followed by RNA-seq CPSF2-BGHcLV29-HepG2ENCSR224XSSCPSF2HepG2ReleasedENCSR462MFN   CRISPRKD% = 52.8,51.2






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CPSF2ENCAB512SARA301-581A1Released, ENCODE4BGHcLV29-33, Released, ENCODE4BGKcLV28-33, Released, ENCODE4
CPSF2ENCAB473NHBA301-582A1Released, ENCODE4BGHcLV29-33, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CPSF2K562CRISPRENCSR887XCLENCSR220DTXCPSF2-BGKcLV28-33,CPSF2-BGKcLV28-34NT-BGKcLV28-1,NT-BGKcLV28-270.5,67.6ReleasedENCODE4
CPSF2HepG2CRISPRENCSR224XSSENCSR462MFNCPSF2-BGHcLV29-33,CPSF2-BGHcLV29-34NT-BGHcLV29-1,NT-BGHcLV29-252.8,51.2ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CPSF2Product_ID: A301-581A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CPSF2-human		 CPSF2-HEPG2-CRISPR-A301-581A.png<br>Caption: Western blot following CRISPR against CPSF2 in HepG2 whole cell lysate using CPSF2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CPSF2. CPSF2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A301-581A_1_CPSF2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CPSF2 specific antibody, A301-581A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CPSF2-specific antibody, A301-581A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CPSF2-K562-CRISPR-A301-581A.png<br>Caption: Western blot following CRISPR against CPSF2 in K562 whole cell lysate using CPSF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CPSF2. CPSF2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
CPSF2Product_ID: A301-582A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CPSF2-human		 Bethyl_A301-582A_1_CPSF2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CPSF2 specific antibody, A301-582A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CPSF2-specific antibody, A301-582A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.








RNA Targets

  • eCLIP assay for CPSF2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • CPSF2 RNA is bound by 12 proteins.  







eCLIP assay for CPSF2 is not avaliable on ENCORE database.





12 proteins are bound to CPSF2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr149260173792601788+3'-UTR,CDS3.788664.51739HepG2BCLAF1
chr149262825692628339+3'-UTR3.099594.62974K562PUM2
chr149263035492630392+3'-UTR4.136673.69433K562UPF1
chr149262904692629141+3'-UTR4.151165.42334HepG2QKI
chr149262906992629143+3'-UTR3.959653.97692K562IGF2BP1
chr149262990492630007+3'-UTR3.541058.10650K562FAM120A
chr149262894392629001+3'-UTR3.310633.07801K562UPF1
chr149262927292629313+3'-UTR3.962074.40891K562FAM120A
chr149262991792630009+3'-UTR3.468954.61367K562UPF1
chr149262894292629003+3'-UTR3.918583.25175K562YBX3
chr149258834392588401+5'-UTR5.550057.56430K562DDX3X
chr149258833192588407+5'-UTR4.766234.66990HepG2DDX3X
chr149262146792621590+CDS3.100664.55847HepG2G3BP1
chr149259741592597474+CDS3.281394.61799HepG2BCLAF1
chr149260180692601839+CDS3.602543.68471HepG2BCLAF1
chr149260035492600442+CDS,intron-Distal,intron-Proximal3.294664.49055K562YBX3
chr149262159492621667+CDS3.913305.42720K562UCHL5
chr149262071892620801+CDS4.160453.36483HepG2G3BP1
chr149260866792608695+CDS3.124075.00751HepG2BCLAF1
chr149262282392622846+CDS,intron-Distal4.624075.03746HepG2BCLAF1
chr149260857692608644+CDS5.130116.58593HepG2BCLAF1
chr149262544992625522+CDS3.062414.79538HepG2BCLAF1
chr149260952692609600+CDS3.756573.12746HepG2GRWD1
chr149262544992625515+CDS3.927923.11149HepG2G3BP1
chr149262748692627523+CDS3.291073.27569HepG2BCLAF1
chr149262159692621668+CDS,intron-Proximal5.242597.04013HepG2BCLAF1
chr149260854792608638+CDS3.969123.31827HepG2GRWD1
chr149262149692621551+CDS4.190616.65960HepG2BCLAF1
chr149260460392604680+CDS3.353485.15500HepG2BCLAF1
chr149261128692611377+intron-Distal3.706453.06005HepG2QKI
chr149261062092610730+intron-Distal4.749706.84124HepG2SUGP2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CPSF2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CPSF2 is not available on ENCORE database.

  • 101 RBPs can affect the levels of CPSF2.  








The Knockdown/knockout RNA-Seq assay for CPSF2 is not available on ENCORE database.





101 RBPs can affect the gene expression of CPSF2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CPSF2ENSG00000165934.81018.281401344.03520-0.400403.186302.12000HepG2ADAR
CPSF2ENSG00000165934.82786.857201920.412100.537104.763204.08920HepG2AKAP1
CPSF2ENSG00000165934.81890.427102404.53610-0.347005.181804.29100HepG2AUH
CPSF2ENSG00000165934.81017.723201501.98210-0.561503.997802.87250HepG2BCCIP
CPSF2ENSG00000165934.8844.925001305.21670-0.627405.180003.98430HepG2BCLAF1
CPSF2ENSG00000165934.81548.747602157.17960-0.478005.294304.50010HepG2BOP1
CPSF2ENSG00000165934.81290.543701522.26840-0.238201.866101.30550HepG2CKAP4
CPSF2ENSG00000165934.81549.070501980.40720-0.354402.818402.12580HepG2CPSF6
CPSF2ENSG00000165934.81147.472101378.25660-0.264402.425701.47520HepG2YBX3
CPSF2ENSG00000165934.81349.535801844.70610-0.450907.034306.09870HepG2CELF1
CPSF2ENSG00000165934.81489.833001983.45930-0.412908.574107.37860HepG2DAZAP1
CPSF2ENSG00000165934.81415.817302248.89830-0.667604.027602.86250HepG2DDX59
CPSF2ENSG00000165934.8986.599601305.35760-0.403903.621402.50770HepG2DHX30
CPSF2ENSG00000165934.81106.197101353.43690-0.291002.894502.17830HepG2DNAJC21
CPSF2ENSG00000165934.8923.080601341.35160-0.5392011.186809.97360HepG2DNAJC2
CPSF2ENSG00000165934.8727.653201519.27740-1.0621010.046208.95730HepG2EIF2S1
CPSF2ENSG00000165934.81689.136001956.84980-0.212202.221401.45730HepG2EIF4G1
CPSF2ENSG00000165934.81346.907001874.52870-0.476904.825103.76790HepG2EIF4G2
CPSF2ENSG00000165934.82138.778702760.86420-0.368306.385405.53430HepG2ETF1
CPSF2ENSG00000165934.81033.313601487.82940-0.525904.731003.64450HepG2EWSR1
CPSF2ENSG00000165934.8703.07570918.92300-0.386303.348102.04900HepG2FAM120A
CPSF2ENSG00000165934.81125.883801569.63220-0.479402.921101.77460HepG2FUS
CPSF2ENSG00000165934.8953.505801571.40600-0.720705.433204.51350HepG2G3BP1
CPSF2ENSG00000165934.8722.25100915.59560-0.342202.102501.32620HepG2GEMIN5
CPSF2ENSG00000165934.81351.032102244.94290-0.732608.607807.52160HepG2GPKOW
CPSF2ENSG00000165934.81051.232301366.40170-0.378303.291902.59540HepG2HNRNPA1
CPSF2ENSG00000165934.81044.760501519.82480-0.540706.014905.02090HepG2HNRNPC
CPSF2ENSG00000165934.81353.067801877.54850-0.472608.611807.43660HepG2HNRNPF
CPSF2ENSG00000165934.81222.349801806.76370-0.5638014.1904012.77510HepG2HNRNPL
CPSF2ENSG00000165934.81698.617202142.39060-0.334902.517601.91270HepG2HNRNPLL
CPSF2ENSG00000165934.81015.630501287.13550-0.341802.289201.42540HepG2HNRNPM
CPSF2ENSG00000165934.81248.868801509.77990-0.273703.237102.58470HepG2HNRNPU
CPSF2ENSG00000165934.81349.217501848.84790-0.454505.891404.87480HepG2IGF2BP1
CPSF2ENSG00000165934.81027.353801542.19780-0.5861014.5514013.02030HepG2ILF2
CPSF2ENSG00000165934.81205.983202184.96660-0.857407.759206.10670HepG2MTPAP
CPSF2ENSG00000165934.81313.886901966.79700-0.582009.928208.51290HepG2NOL12
CPSF2ENSG00000165934.81350.301001575.90900-0.222902.133501.58850HepG2PCBP1
CPSF2ENSG00000165934.8945.036501323.06990-0.485403.138901.34880HepG2PHF6
CPSF2ENSG00000165934.8893.567201346.61080-0.591709.664608.33240HepG2PPIL4
CPSF2ENSG00000165934.81670.152001993.06020-0.255003.756103.08810HepG2PUM1
CPSF2ENSG00000165934.81538.697201991.59380-0.372204.250703.43020HepG2PUM2
CPSF2ENSG00000165934.82175.510101434.080800.601103.430002.64740HepG2RAVER1
CPSF2ENSG00000165934.81567.171401955.99250-0.319703.064502.16610HepG2RBM15
CPSF2ENSG00000165934.81035.103601344.55200-0.377405.229704.39460HepG2SAFB2
CPSF2ENSG00000165934.81433.605501800.25930-0.328605.172804.26180HepG2SLBP
CPSF2ENSG00000165934.81722.442902606.23560-0.5975016.7778015.81720HepG2SRFBP1
CPSF2ENSG00000165934.82564.224201742.600100.5572013.2798012.40940HepG2SRSF1
CPSF2ENSG00000165934.81658.455802281.71520-0.460303.302002.19670HepG2SRSF3
CPSF2ENSG00000165934.81548.367701853.77260-0.259702.176801.61440HepG2SRSF5
CPSF2ENSG00000165934.81158.229201844.63430-0.6714016.0545014.61830HepG2SRSF7
CPSF2ENSG00000165934.81019.704001470.12610-0.527803.714002.94690HepG2SUPV3L1
CPSF2ENSG00000165934.81303.434302088.17560-0.679909.322408.10710HepG2TARDBP
CPSF2ENSG00000165934.8903.388201467.20020-0.6996012.0715011.15530HepG2TIAL1
CPSF2ENSG00000165934.8998.308801480.13680-0.568208.368007.47160HepG2TRIM56
CPSF2ENSG00000165934.82045.953301595.723000.358503.312602.63030HepG2U2AF1
CPSF2ENSG00000165934.8634.94690868.51140-0.451902.952702.17130HepG2UCHL5
CPSF2ENSG00000165934.8864.790301346.42710-0.638703.926602.72040HepG2UPF2
CPSF2ENSG00000165934.8904.527801447.17510-0.678006.559005.46000HepG2XRN2
CPSF2ENSG00000165934.81187.396901540.09570-0.375206.154405.21460HepG2ZC3H8
CPSF2ENSG00000165934.81847.895102326.58470-0.332305.194304.45710K562AGGF1
CPSF2ENSG00000165934.81991.201002556.43250-0.360503.596702.43960K562AKAP8
CPSF2ENSG00000165934.81808.786502420.88430-0.420504.602903.75300K562AKAP8L
CPSF2ENSG00000165934.81757.586803356.43520-0.9333019.0224017.64950K562AQR
CPSF2ENSG00000165934.81698.434502020.44170-0.250502.620701.35160K562BCCIP
CPSF2ENSG00000165934.81559.295701952.48130-0.324404.734203.45920K562BCLAF1
CPSF2ENSG00000165934.81642.542402173.86280-0.404302.447501.63140K562BOP1
CPSF2ENSG00000165934.82125.903502803.45920-0.399103.273701.69950K562BUD13
CPSF2ENSG00000165934.8662.59030886.43870-0.419903.135401.99660K562CCAR1
CPSF2ENSG00000165934.82904.494203562.37130-0.294603.237602.03450K562CPEB4
CPSF2ENSG00000165934.81782.475902311.54550-0.375002.526201.77560K562CPSF6
CPSF2ENSG00000165934.81104.436601481.24810-0.423505.292103.76490K562CSTF2
CPSF2ENSG00000165934.81169.942401514.23880-0.372203.609902.40150K562DDX3X
CPSF2ENSG00000165934.84491.791703686.060300.285104.779003.95620K562DDX47
CPSF2ENSG00000165934.81245.939201535.21840-0.301203.156401.73410K562DDX6
CPSF2ENSG00000165934.81323.485801663.17730-0.329604.440702.88410K562EIF2S1
CPSF2ENSG00000165934.82137.455602727.23900-0.351505.118704.18330K562EIF2S2
CPSF2ENSG00000165934.82006.648901671.728900.263302.551101.78640K562EIF3G
CPSF2ENSG00000165934.81095.006101581.97470-0.530806.079004.87270K562EIF4A3
CPSF2ENSG00000165934.81174.242201424.62430-0.278902.635401.51980K562EIF4B
CPSF2ENSG00000165934.81054.547701380.88300-0.389004.457702.75800K562EWSR1
CPSF2ENSG00000165934.81473.209001853.67340-0.331403.190101.82020K562EXOSC5
CPSF2ENSG00000165934.82062.594802893.17530-0.488205.811904.55080K562FIP1L1
CPSF2ENSG00000165934.81576.543102351.73810-0.577004.731203.61050K562FMR1
CPSF2ENSG00000165934.81518.804701993.22130-0.392202.774501.85880K562FTO
CPSF2ENSG00000165934.81549.553402207.04170-0.510304.022203.06960K562GPKOW
CPSF2ENSG00000165934.82397.369103634.56970-0.600305.256304.20940K562GRSF1
CPSF2ENSG00000165934.81835.321802740.55300-0.578402.442901.31910K562HNRNPA2B1
CPSF2ENSG00000165934.81002.471301719.83580-0.7787011.6105010.46080K562HNRNPC
CPSF2ENSG00000165934.82633.986403331.22820-0.338803.610102.38150K562HNRNPL
CPSF2ENSG00000165934.81306.106301884.31670-0.528803.827102.71670K562KRR1
CPSF2ENSG00000165934.81736.862902221.53010-0.355103.532302.57710K562MAGOH
CPSF2ENSG00000165934.81580.979702154.28320-0.446403.287702.33210K562METAP2
CPSF2ENSG00000165934.81951.343202459.06870-0.333605.581404.45700K562MTPAP
CPSF2ENSG00000165934.82223.258002732.29730-0.297406.448705.37280K562NSUN2
CPSF2ENSG00000165934.81538.131302398.27470-0.640802.906801.88480K562PABPN1
CPSF2ENSG00000165934.81168.581201678.92620-0.522805.105403.08690K562PHF6
CPSF2ENSG00000165934.81927.468302506.19220-0.378802.934701.33060K562PKM
CPSF2ENSG00000165934.82389.618703284.34890-0.458805.015803.85690K562PPIG
CPSF2ENSG00000165934.81604.637802852.33030-0.829903.974702.10920K562PPP1R8
CPSF2ENSG00000165934.8956.898301541.95240-0.688307.185205.60710K562PSIP1
CPSF2ENSG00000165934.83749.746604969.27300-0.406206.511405.02260K562PUM2
CPSF2ENSG00000165934.81912.801902401.08600-0.328002.769502.22620K562PUS1
CPSF2ENSG00000165934.82031.428302339.30340-0.203601.901501.39380K562RBM15
CPSF2ENSG00000165934.81709.972602069.50320-0.275302.086801.36210K562RBM22
CPSF2ENSG00000165934.81374.588701787.17770-0.378703.628301.66640K562RECQL
CPSF2ENSG00000165934.81237.976502193.89780-0.825503.559502.14260K562SF1
CPSF2ENSG00000165934.82073.657002696.00420-0.378606.069404.89880K562SF3A3
CPSF2ENSG00000165934.81561.474202098.66700-0.426602.983301.97230K562SF3B1
CPSF2ENSG00000165934.81387.499101830.04260-0.399404.707703.58100K562SF3B4
CPSF2ENSG00000165934.8972.837601333.39230-0.454804.193702.85560K562SRFBP1
CPSF2ENSG00000165934.82024.754702762.10760-0.4480011.116309.39050K562SRPK2
CPSF2ENSG00000165934.81501.620601998.41330-0.412303.955902.66310K562SRSF5
CPSF2ENSG00000165934.81642.521901968.83020-0.261403.072501.83420K562SRSF7
CPSF2ENSG00000165934.81169.023201576.30630-0.431203.086402.01280K562SUPV3L1
CPSF2ENSG00000165934.81522.818902141.42540-0.491805.094404.11940K562TARDBP
CPSF2ENSG00000165934.81520.040502065.66030-0.442505.488304.27360K562TIA1
CPSF2ENSG00000165934.81019.223901483.13570-0.541204.921203.64090K562TIAL1
CPSF2ENSG00000165934.81587.026702027.15030-0.353103.183401.99930K562TRA2A
CPSF2ENSG00000165934.81132.104001575.31400-0.476604.912403.56590K562TRIM56
CPSF2ENSG00000165934.82238.612202897.10870-0.372007.430006.32220K562UPF1
CPSF2ENSG00000165934.82928.188402577.796900.183802.301801.52380K562WDR43
CPSF2ENSG00000165934.81321.222101698.44560-0.362302.618601.45330K562XRN2
CPSF2ENSG00000165934.82783.914503781.06280-0.441705.357904.09150K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CPSF2 is not available on ENCORE database.





5 RBPs can affect the splicing of CPSF2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECPSF2ENSG00000165934.8chr14+926228229262297592621466926216679262400292624228-0.253000.000000.00000excludedK562SF3B4
SECPSF2ENSG00000165934.8chr14+926003549260051492597294925974779260059392600699-0.058000.002230.02407excludedK562AQR
SECPSF2ENSG00000165934.8chr14+926228229262297592621466926216679262400292624228-0.108000.000340.00946excludedK562U2AF1
SECPSF2ENSG00000165934.8chr14+926228229262297592621466926216679262400292624228-0.069000.000000.00000excludedK562HNRNPL
SECPSF2ENSG00000165934.8chr14+926228229262297592621466926216679262400292624228-0.087000.001570.06266excludedK562HNRNPK
A3SSCPSF2ENSG00000165934.8chr14+925924659259252492592475925925249258828092588472-0.117000.000680.03859shorterK562SF3B4
A3SSCPSF2ENSG00000165934.8chr14+9260031892600498926003549260049892597251925974770.086000.000030.00029longerK562AQR
MXECPSF2ENSG00000165934.8chr14+9260035492600514926005939260071892597294925974779260171092601840-0.100000.000310.003232includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CPSF2 is not avaliable on ENCORE database.