AHCYL2

General Information

Gene Name       : AHCYL2
Gene ID             : ENSG00000158467.16
Essential Gene : No

Functions

Localization

Domains (AlphaFold: AHCYL2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878AHCYL2ENSG00000158467.166.165.265.71ENCSR000AEF
HepG2AHCYL2ENSG00000158467.166.356.536.44ENCSR985KAT
K562AHCYL2ENSG00000158467.163.454.153.80ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq AHCYL2-BGKcLV26-K562ENCSR700JXJAHCYL2K562ReleasedENCSR900GQL   CRISPRKD% = 78.1,85.3
knockdown followed by RNA-seq AHCYL2-BGHcLV25-HepG2ENCSR363NMOAHCYL2HepG2ReleasedENCSR953VMB   CRISPRKD% = 70.2,67.8






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
AHCYL2ENCAB809CIXA300-262A2Released, ENCODE4BGHcLV25-21, Released, ENCODE4BGKcLV26-17, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
AHCYL2K562CRISPRENCSR700JXJENCSR900GQLAHCYL2-BGKcLV26-17,AHCYL2-BGKcLV26-18NT-BGKcLV26-1,NT-BGKcLV26-278.1,85.3ReleasedENCODE4
AHCYL2HepG2CRISPRENCSR363NMOENCSR953VMBAHCYL2-BGHcLV25-21,AHCYL2-BGHcLV25-22NT-BGHcLV25-1,NT-BGHcLV25-270.2,67.8ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
AHCYL2Product_ID: A300-262A
Lot_ID: 2
Source: Bethyl Labs
Target Name: AHCYL2-human		 AHCYL2-HEPG2-CRISPR-A300-262A.png<br>Caption: Western blot following CRISPR against AHCYL2 in HepG2 whole cell lysate using AHCYL2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AHCYL2. AHCYL2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A300-262A_2_AHCYL2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the AHCYL2 specific antibody, A300-262A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the AHCYL2-specific antibody, A300-262A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 AHCYL2-K562-CRISPR-A300-262A.png<br>Caption: Western blot following CRISPR against AHCYL2 in K562 whole cell lysate using AHCYL2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against AHCYL2. AHCYL2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for AHCYL2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • AHCYL2 RNA is bound by 14 proteins.  







eCLIP assay for AHCYL2 is not avaliable on ENCORE database.





14 proteins are bound to AHCYL2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr7129069568129069648+3'-UTR3.721423.68753HepG2PCBP2
chr7128864863128864962+5'-UTR,CDS4.375144.14518HepG2GTF2F1
chr7128864863128864912+5'-UTR3.970293.05512HepG2PRPF4
chr7128864863128864980+5'-UTR,CDS5.6760311.61399K562GTF2F1
chr7128864903128865068+5'-UTR,CDS3.394994.25541HepG2GRWD1
chr7129043233129043327+CDS,intron-Proximal4.922596.04243HepG2BCLAF1
chr7128865048128865108+CDS4.177085.59994HepG2XPO5
chr7128898857128898928+intron-Distal4.622485.58765HepG2QKI
chr7128873843128873946+intron-Distal3.491524.83120HepG2MATR3
chr7128873794128873933+intron-Distal3.571948.57718HepG2PTBP1
chr7128875102128875144+intron-Distal4.687216.08205HepG2MATR3
chr7128873902128873956+intron-Distal4.708466.58366HepG2SUGP2
chr7128980665128980825+intron-Distal4.649296.09994K562KHSRP
chr7128914276128914333+intron-Distal5.062306.37401HepG2RBFOX2
chr7128951477128951584+intron-Distal5.414547.97897HepG2QKI
chr7128873891128873946+intron-Distal4.557715.76788K562MATR3
chr7128874958128875001+intron-Distal4.039483.27829HepG2MATR3
chr7128949464128949552+intron-Distal3.265553.84025HepG2SUGP2
chr7128874771128874873+intron-Distal4.368963.27829HepG2MATR3
chr7128898963128899030+intron-Distal4.6632912.19689HepG2QKI
chr7128882642128882716+intron-Distal4.632585.04993HepG2QKI
chr7128941840128941892+intron-Distal3.957253.01445K562EXOSC5
chr7128874671128874865+intron-Distal3.152054.54387HepG2PTBP1
chr7128922709128922793+intron-Distal4.299465.04879HepG2SUGP2
chr7128873893128873941+intron-Distal4.848139.53330K562PTBP1
chr7128880132128880229+intron-Distal4.078453.80299K562HNRNPL
chr7128978676128978752+intron-Distal3.870883.06150K562KHSRP
chr7128949792128949908+intron-Distal3.501554.32634HepG2PTBP1
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for AHCYL2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for AHCYL2 is not available on ENCORE database.

  • 65 RBPs can affect the levels of AHCYL2.  








The Knockdown/knockout RNA-Seq assay for AHCYL2 is not available on ENCORE database.





65 RBPs can affect the gene expression of AHCYL2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
AHCYL2ENSG00000158467.12276.58250479.54830-0.794009.555508.56470HepG2ACO1
AHCYL2ENSG00000158467.12445.90380868.32700-0.961509.351908.43750HepG2AKAP1
AHCYL2ENSG00000158467.12355.29940481.83120-0.439503.438702.59450HepG2AKAP8L
AHCYL2ENSG00000158467.12460.95110718.48570-0.640305.090604.04050HepG2ASCC1
AHCYL2ENSG00000158467.12547.07700415.578500.396502.348301.79140HepG2BOP1
AHCYL2ENSG00000158467.12322.38020442.23420-0.456002.643001.56160HepG2CPSF7
AHCYL2ENSG00000158467.12464.46020572.89960-0.302701.938701.38220HepG2CELF1
AHCYL2ENSG00000158467.12576.28930443.530000.377703.418002.50250HepG2DDX19B
AHCYL2ENSG00000158467.12573.91120418.965300.453902.941902.24490HepG2DDX21
AHCYL2ENSG00000158467.12581.47930409.755300.504903.792202.71040HepG2DDX24
AHCYL2ENSG00000158467.12287.87470412.91630-0.520402.550001.80310HepG2DDX55
AHCYL2ENSG00000158467.12320.90430714.46840-1.1547012.1985011.00920HepG2DDX6
AHCYL2ENSG00000158467.12581.19390458.807100.341002.414601.77470HepG2DKC1
AHCYL2ENSG00000158467.12349.82500450.41930-0.364602.640102.00100HepG2DNAJC2
AHCYL2ENSG00000158467.12499.22440686.92610-0.460502.868102.26490HepG2EIF2S1
AHCYL2ENSG00000158467.12505.49630759.17770-0.586703.987803.22420HepG2EIF4A3
AHCYL2ENSG00000158467.12540.21070739.08270-0.452202.180601.56770HepG2EIF4B
AHCYL2ENSG00000158467.12771.21130607.726200.343603.007402.14050HepG2EIF4G1
AHCYL2ENSG00000158467.12528.40100765.84640-0.535403.833703.02950HepG2FXR1
AHCYL2ENSG00000158467.12402.44720710.50430-0.820007.423506.35780HepG2G3BP1
AHCYL2ENSG00000158467.12280.18700510.28710-0.864907.679806.57140HepG2HNRNPC
AHCYL2ENSG00000158467.12518.40310719.28770-0.472503.250402.49390HepG2HNRNPK
AHCYL2ENSG00000158467.12551.31200722.23690-0.389602.283801.66380HepG2IGF2BP2
AHCYL2ENSG00000158467.12472.84070726.40060-0.619403.846202.64410HepG2KHSRP
AHCYL2ENSG00000158467.12511.14370766.11320-0.583804.426503.59920HepG2MAGOH
AHCYL2ENSG00000158467.12414.14330770.42250-0.895508.222807.18400HepG2MATR3
AHCYL2ENSG00000158467.12385.70320610.80830-0.663206.962005.75980HepG2NOL12
AHCYL2ENSG00000158467.12190.34350379.20180-0.994405.188603.66580HepG2NONO
AHCYL2ENSG00000158467.12254.80480502.60260-0.980005.975804.63440HepG2PABPC1
AHCYL2ENSG00000158467.12352.62820501.74230-0.508804.342003.31070HepG2PARN
AHCYL2ENSG00000158467.12596.00980763.96000-0.358203.334202.68160HepG2PCBP1
AHCYL2ENSG00000158467.12330.55050452.12340-0.451803.273502.47760HepG2PPIL4
AHCYL2ENSG00000158467.12652.38310476.750700.452404.306703.36560HepG2PRPF4
AHCYL2ENSG00000158467.12432.48030318.492500.441302.056801.35620HepG2PRPF8
AHCYL2ENSG00000158467.12747.48570583.077000.358302.485801.91680HepG2PUF60
AHCYL2ENSG00000158467.12286.65940618.97010-1.1105020.6264019.31030HepG2PUM1
AHCYL2ENSG00000158467.12770.13110488.456300.656809.735608.69870HepG2PUS1
AHCYL2ENSG00000158467.12242.71280492.88530-1.022004.722803.81550HepG2RAVER1
AHCYL2ENSG00000158467.12609.39880865.12280-0.505505.880305.13890HepG2NELFE
AHCYL2ENSG00000158467.12559.38470809.33500-0.532906.307105.47530HepG2SF3B4
AHCYL2ENSG00000158467.12419.06010526.42210-0.329102.475701.86260HepG2SMN1
AHCYL2ENSG00000158467.121061.25750780.215700.443705.442704.80470HepG2SRFBP1
AHCYL2ENSG00000158467.12388.28160687.25750-0.8237013.0196011.97940HepG2SRSF9
AHCYL2ENSG00000158467.12832.56750677.376900.297502.538902.04230HepG2SSB
AHCYL2ENSG00000158467.12537.12370753.22590-0.487802.703901.39550HepG2SUGP2
AHCYL2ENSG00000158467.12595.03220402.260400.564704.180803.31210HepG2TARDBP
AHCYL2ENSG00000158467.12377.91720496.96610-0.395103.035102.45100HepG2TRIM56
AHCYL2ENSG00000158467.12606.30790463.934900.386001.869801.34260HepG2U2AF1
AHCYL2ENSG00000158467.12585.26840470.493500.314802.386501.75730HepG2ZC3H8
AHCYL2ENSG00000158467.12291.83750647.49060-1.1497023.5483022.34330HepG2ZNF622
AHCYL2ENSG00000158467.12727.64270416.555800.8046012.1918010.93060K562DDX47
AHCYL2ENSG00000158467.12354.90290239.806900.565503.023102.12700K562FMR1
AHCYL2ENSG00000158467.12197.40840278.66020-0.497302.589802.00030K562FXR1
AHCYL2ENSG00000158467.1284.63350214.09860-1.339008.719507.70970K562HNRNPC
AHCYL2ENSG00000158467.12165.65670259.21540-0.646002.893901.93880K562HNRNPM
AHCYL2ENSG00000158467.12502.75320346.082900.538604.620703.46090K562HNRNPU
AHCYL2ENSG00000158467.12158.49470312.53640-0.979608.417406.70560K562MATR3
AHCYL2ENSG00000158467.12314.88980219.669200.519402.615801.76770K562METAP2
AHCYL2ENSG00000158467.12130.99060227.53480-0.796604.027702.39470K562NONO
AHCYL2ENSG00000158467.12502.95470272.837200.882307.028805.70000K562PCBP3
AHCYL2ENSG00000158467.12408.54430209.270000.965004.084603.10820K562POLR2G
AHCYL2ENSG00000158467.12265.32190357.75830-0.431202.066701.36110K562PPIG
AHCYL2ENSG00000158467.12451.30710633.70230-0.489704.332802.97220K562PUM1
AHCYL2ENSG00000158467.12211.70300291.79620-0.462902.250901.49720K562RBM22
AHCYL2ENSG00000158467.12577.88230228.750901.3369012.1666010.43160K562RPS19
AHCYL2ENSG00000158467.12219.92040321.85910-0.549403.611602.68720K562SF3A3
AHCYL2ENSG00000158467.12164.52680304.66560-0.888903.396501.89810K562SFPQ
AHCYL2ENSG00000158467.12146.95420217.40510-0.565002.219001.31500K562SUPV3L1
AHCYL2ENSG00000158467.12213.94740301.86930-0.496702.416501.75270K562TARDBP
AHCYL2ENSG00000158467.12173.86230273.02730-0.651103.126901.95250K562U2AF1
AHCYL2ENSG00000158467.12158.83630281.95710-0.827905.895904.72480K562U2AF2
AHCYL2ENSG00000158467.12580.35050345.896400.746509.264208.04360K562UPF1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for AHCYL2 is not available on ENCORE database.






No RBPs affect the alternative splcing events of AHCYL2 on ENCORE database.





The Knockdown/knockout RNA-Seq assay for AHCYL2 is not avaliable on ENCORE database.