RBBP5

General Information

Gene Name       : RBBP5
Gene ID             : ENSG00000117222.13
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RBBP5)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RBBP5ENSG00000117222.1313.8711.5912.73ENCSR000AEF
HepG2RBBP5ENSG00000117222.135.999.947.96ENCSR985KAT
K562RBBP5ENSG00000117222.1318.8921.4720.18ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RBBP513171S1Submitted, ENCORE2BGHcLV43-47, Submitted, ENCORE2BGKcLV42-75, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RBBP5K562CRISPRRBBP5-BGKcLV42-75,RBBP5-BGKcLV42-76NT-BGKcLV42-1,NT-BGKcLV42-293.4,91.9SequencedENCORE2
RBBP5K562CRISPRBGKcLV42-77,BGKcLV42-78BGKcLV42-1,BGKcLV42-277.7,75.0NotSatisfiedENCORE2
RBBP5HepG2CRISPRRBBP5-BGHcLV43-47,RBBP5-BGHcLV43-48NT-BGHcLV43-1,NT-BGHcLV43-273.9,70.2SequencedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBBP5Product_ID: 13171S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBBP5-human		 RBBP5-HEPG2-CRISPR-13171S.png<br>Caption: Western blot following CRISPR against RBBP5 in HepG2 whole cell lysate using RBBP5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBBP5. RBBP5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13171S_1_RBBP5.png<br>Caption: IP-WB analysis of 13171S whole cell lysate using the RBBP5 specific antibody, 13171S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBBP5-specific antibody, 13171S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBBP5-K562-CRISPR-13171S.png<br>Caption: Western blot following CRISPR against RBBP5 in K562 whole cell lysate using RBBP5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBBP5. RBBP5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RBBP5 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RBBP5 RNA is bound by 26 proteins.  







eCLIP assay for RBBP5 is not avaliable on ENCORE database.





26 proteins are bound to RBBP5 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr1205057902205057962-3'-UTR,CDS,intron-Distal5.296208.59716K562BUD13
chr1205056758205056846-3'-UTR,intron-Distal3.053284.58951K562PUM2
chr1205056864205056948-3'-UTR,intron-Distal3.447884.00685K562TIA1
chr1205056121205056184-3'-UTR,intron-Distal3.510937.60286K562UPF1
chr1205057372205057445-3'-UTR,intron-Distal3.810756.14589K562IGF2BP1
chr1205055862205055948-3'-UTR,intron-Distal4.113898.99604K562UPF1
chr1205055317205055356-3'-UTR,intron-Distal5.498968.52455K562PABPC4
chr1205055692205055718-3'-UTR,intron-Distal3.771964.50550K562UPF1
chr1205056565205056672-3'-UTR,intron-Distal3.6590212.19870K562UPF1
chr1205057839205057895-3'-UTR,intron-Distal3.411014.22460K562RBM15
chr1205057908205057966-3'-UTR,CDS,intron-Distal3.357204.57930K562GPKOW
chr1205057174205057241-3'-UTR,intron-Distal3.222264.05694K562IGF2BP1
chr1205057366205057435-3'-UTR,intron-Distal3.131345.59934K562FAM120A
chr1205090936205091028-5'-UTR,CDS,intron-Distal4.100035.64484HepG2PRPF8
chr1205090982205091120-5'-UTR,CDS,intron-Distal,intron-Proximal3.6945911.03951K562DDX3X
chr1205065903205065930-CDS,intron-Distal3.103354.00731K562UCHL5
chr1205065853205065906-CDS,intron-Distal4.176484.67299K562DDX24
chr1205074257205074340-CDS,intron-Distal,intron-Proximal5.075184.90235K562SF3B4
chr1205066487205066524-CDS,intron-Distal,intron-Proximal4.8077313.00315K562ZNF622
chr1205066028205066039-CDS,intron-Distal3.255783.69481K562UCHL5
chr1205069288205069367-CDS,intron-Distal,intron-Proximal3.359823.57857HepG2BCLAF1
chr1205069054205069164-CDS,intron-Distal3.982153.34184HepG2GRWD1
chr1205068115205068189-CDS,intron-Distal,intron-Proximal3.502903.06344K562TRA2A
chr1205068935205069024-CDS,intron-Distal,intron-Proximal4.106564.89018K562EFTUD2
chr1205066408205066456-CDS,intron-Distal,intron-Proximal4.131423.31283K562PRPF8
chr1205068115205068165-CDS,intron-Distal,intron-Proximal3.251639.25291K562UCHL5
chr1205065835205065875-CDS,intron-Distal3.787974.74268K562UCHL5
chr1205069145205069265-CDS,intron-Distal,intron-Proximal3.565098.13137K562EFTUD2
chr1205065875205065903-CDS,intron-Distal3.447864.89070K562UCHL5
chr1205068116205068180-CDS,intron-Distal,intron-Proximal4.2227611.72604K562ZNF622
chr1205064033205064195-CDS,intron-Distal,intron-Proximal3.922307.45570K562ZNF800
chr1205066454205066487-CDS,intron-Distal5.068367.69747K562ZNF622
chr1205064090205064194-CDS,intron-Distal,intron-Proximal3.4159310.62736K562ZNF622
chr1205065855205065933-CDS,intron-Distal4.2227611.72604K562ZNF622
chr1205064104205064193-CDS,intron-Distal,intron-Proximal4.650843.71570HepG2BCLAF1
chr1205066453205066501-CDS,intron-Distal,intron-Proximal3.7205810.26098K562UCHL5
chr1205084952205085040-CDS,intron-Distal,intron-Proximal3.464545.46476K562AQR
chr1205066480205066524-CDS,intron-Distal,intron-Proximal3.641594.06415K562TRA2A
chr1205066501205066524-CDS,intron-Distal,intron-Proximal3.352907.85158K562UCHL5
chr1205089854205089913-intron-Distal3.845793.21162HepG2HNRNPC
chr1205081800205081935-intron-Distal3.623975.95946K562KHSRP
chr1205066411205066444-intron-Distal,intron-Proximal5.430987.63570K562AQR
chr1205062020205062120-intron-Distal4.092084.43126K562KHDRBS1
chr1205090879205090983-intron-Distal4.582407.50107K562AQR
chr1205074904205074992-intron-Distal4.061823.90241K562MATR3
chr1205074961205075023-intron-Distal4.960948.41046K562HNRNPM
chr1205059614205059803-intron-Distal4.324339.70733K562KHSRP
chr1205066444205066454-intron-Distal,intron-Proximal4.278566.77156K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RBBP5 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RBBP5 is not available on ENCORE database.

  • 64 RBPs can affect the levels of RBBP5.  








The Knockdown/knockout RNA-Seq assay for RBBP5 is not available on ENCORE database.





64 RBPs can affect the gene expression of RBBP5 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RBBP5ENSG00000117222.9544.08550447.432100.282101.925801.40150HepG2ACO1
RBBP5ENSG00000117222.9928.89800573.337300.696005.567304.84370HepG2AKAP1
RBBP5ENSG00000117222.9285.66970474.41190-0.731805.024803.98150HepG2ASCC1
RBBP5ENSG00000117222.9238.53240461.44650-0.952005.195503.90620HepG2BCCIP
RBBP5ENSG00000117222.9245.08720401.02050-0.710403.605602.58810HepG2BCLAF1
RBBP5ENSG00000117222.9267.17680465.95510-0.802405.844804.87840HepG2CPSF6
RBBP5ENSG00000117222.9425.30860527.90300-0.311802.157001.57810HepG2CELF1
RBBP5ENSG00000117222.9432.90470567.71410-0.391103.699702.86320HepG2DAZAP1
RBBP5ENSG00000117222.9725.02890549.264100.400402.339301.75900HepG2DDX3X
RBBP5ENSG00000117222.9332.98870529.12320-0.668102.991301.97670HepG2DDX59
RBBP5ENSG00000117222.9265.07960386.45080-0.543903.886103.06470HepG2DNAJC21
RBBP5ENSG00000117222.9238.84020453.58460-0.925306.023805.16720HepG2EIF2S1
RBBP5ENSG00000117222.9335.80810501.32130-0.578102.563901.94780HepG2EIF4A3
RBBP5ENSG00000117222.9335.16290536.46580-0.678605.640704.50080HepG2EIF4G2
RBBP5ENSG00000117222.9364.28980497.93980-0.450902.439801.60520HepG2G3BP2
RBBP5ENSG00000117222.9359.14710528.22210-0.556603.257902.56830HepG2GPKOW
RBBP5ENSG00000117222.9260.93560433.98230-0.733905.265804.33300HepG2HNRNPC
RBBP5ENSG00000117222.9470.41380597.18050-0.344202.340101.67700HepG2HNRNPF
RBBP5ENSG00000117222.9357.50510517.17100-0.532705.415004.49850HepG2HNRNPL
RBBP5ENSG00000117222.9304.15570504.08970-0.728905.339604.49480HepG2HNRNPLL
RBBP5ENSG00000117222.9431.49480588.05910-0.446603.093502.33090HepG2IGF2BP1
RBBP5ENSG00000117222.9352.70300440.36560-0.320202.122501.47070HepG2ILF2
RBBP5ENSG00000117222.9330.16890479.62820-0.538702.621301.64780HepG2KHSRP
RBBP5ENSG00000117222.9234.48160436.87510-0.897707.365705.45810HepG2LIN28B
RBBP5ENSG00000117222.9330.55840514.04990-0.637003.095702.06710HepG2MTPAP
RBBP5ENSG00000117222.9325.59790426.71650-0.390202.558901.74150HepG2PARN
RBBP5ENSG00000117222.9271.37650384.51630-0.502703.496102.67750HepG2PPIL4
RBBP5ENSG00000117222.9831.22350643.570200.369003.211102.66380HepG2RBM22
RBBP5ENSG00000117222.9624.47300498.660200.324502.268901.76900HepG2NELFE
RBBP5ENSG00000117222.9301.30870383.91950-0.349602.075201.50440HepG2SAFB2
RBBP5ENSG00000117222.9906.97160483.757200.906707.867006.68900HepG2SF3A3
RBBP5ENSG00000117222.9918.69160726.887900.337703.540802.99580HepG2SRFBP1
RBBP5ENSG00000117222.9807.51720486.920100.7297010.560109.76820HepG2SRSF1
RBBP5ENSG00000117222.9317.24780451.66700-0.509602.015901.42500HepG2SUPV3L1
RBBP5ENSG00000117222.9373.93280491.26340-0.393702.076001.43560HepG2TARDBP
RBBP5ENSG00000117222.9302.96840418.87330-0.467303.002402.48480HepG2TIAL1
RBBP5ENSG00000117222.9300.13250422.65310-0.493904.014003.35960HepG2TRIM56
RBBP5ENSG00000117222.9676.18990445.023700.603404.486003.69520HepG2U2AF1
RBBP5ENSG00000117222.9135.81510266.84690-0.974405.899304.78120HepG2UCHL5
RBBP5ENSG00000117222.9272.94810444.61520-0.703903.812002.96930HepG2XRN2
RBBP5ENSG00000117222.9381.96350471.83850-0.304902.074501.58190HepG2ZNF622
RBBP5ENSG00000117222.93761.574601804.138101.0599024.5383022.98010K562AQR
RBBP5ENSG00000117222.9845.433901198.99100-0.504106.950304.74530K562BCCIP
RBBP5ENSG00000117222.9920.723601158.66910-0.331603.678102.56450K562BCLAF1
RBBP5ENSG00000117222.91412.828101756.51570-0.314102.535501.47750K562CPEB4
RBBP5ENSG00000117222.9855.910301055.90260-0.302902.222701.51300K562CPSF6
RBBP5ENSG00000117222.9652.16040879.02320-0.430704.042402.73340K562CSTF2
RBBP5ENSG00000117222.9853.289301089.29720-0.352303.024702.19280K562EIF3G
RBBP5ENSG00000117222.9623.86360857.92250-0.459603.157501.93770K562FKBP4
RBBP5ENSG00000117222.91538.735501092.881700.493507.679906.73920K562FXR1
RBBP5ENSG00000117222.91459.282901984.97210-0.443904.497303.53820K562GRSF1
RBBP5ENSG00000117222.9661.40620988.33430-0.579506.090205.23770K562HNRNPC
RBBP5ENSG00000117222.91807.871301361.265900.409203.327001.95670K562HNRNPK
RBBP5ENSG00000117222.9612.627601002.31650-0.710307.350104.77220K562LIN28B
RBBP5ENSG00000117222.91055.742701429.51390-0.437304.629603.40680K562MATR3
RBBP5ENSG00000117222.91083.367901354.34180-0.322103.814202.87800K562MTPAP
RBBP5ENSG00000117222.91281.156501504.80430-0.232102.931302.16700K562NSUN2
RBBP5ENSG00000117222.9729.96880973.05220-0.414702.821201.38400K562NUSAP1
RBBP5ENSG00000117222.9740.16420964.89240-0.382502.945601.46810K562PHF6
RBBP5ENSG00000117222.91298.861701638.70580-0.335302.565801.78090K562PPIG
RBBP5ENSG00000117222.91674.189501024.843200.708009.169307.80100K562PRPF8
RBBP5ENSG00000117222.9585.42360886.13490-0.598005.787204.40620K562PSIP1
RBBP5ENSG00000117222.93327.784002467.437500.431404.696203.63650K562PUF60
RBBP5ENSG00000117222.9646.95840808.43320-0.321502.234601.34710K562RCC2
RBBP5ENSG00000117222.91407.284301147.964500.293702.631101.79920K562SF3B4
RBBP5ENSG00000117222.9540.22560766.37820-0.504503.895602.61520K562SRFBP1
RBBP5ENSG00000117222.9816.699801044.26340-0.354602.726301.68690K562SRSF4
RBBP5ENSG00000117222.91018.812901288.50940-0.338802.567901.56280K562SRSF5
RBBP5ENSG00000117222.9652.24590905.42350-0.473203.875202.71910K562TRIM56
RBBP5ENSG00000117222.91616.103901070.116300.594704.835003.30490K562U2AF1
RBBP5ENSG00000117222.91640.102301105.230100.569308.443007.02640K562U2AF2
RBBP5ENSG00000117222.9753.462701050.30780-0.479203.638902.45800K562UCHL5
RBBP5ENSG00000117222.91128.298501595.57520-0.499909.089707.88090K562UPF1
RBBP5ENSG00000117222.9833.933301365.28450-0.7112010.775408.99860K562WDR3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RBBP5 is not available on ENCORE database.





6 RBPs can affect the splicing of RBBP5 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERBBP5ENSG00000117222.9chr1-205084985205085011205083916205084089205090982205091143-0.204000.004820.07193excludedK562SF3B4
SERBBP5ENSG00000117222.9chr1-205084985205085011205083916205084089205090982205091143-0.157000.000900.01116excludedK562AQR
SERBBP5ENSG00000117222.9chr1-205068116205068234205066453205066523205068868205068940-0.131000.000000.00004excludedK562AQR
SERBBP5ENSG00000117222.9chr1-205083916205084089205074155205074296205090982205091094-0.085000.003770.05004excludedK562PUS1
SERBBP5ENSG00000117222.9chr1-205084985205085011205083916205084089205090982205091143-0.334000.000210.00611excludedK562U2AF1
SERBBP5ENSG00000117222.9chr1-205084985205085011205083916205084089205090982205091143-0.238000.002500.04185excludedK562U2AF2
A3SSRBBP5ENSG00000117222.9chr1-2050641142050643292050641142050641922050658092050660140.108000.000000.00000longerK562AQR
A3SSRBBP5ENSG00000117222.9chr1-2050664532050666972050664532050665232050681162050682510.055000.000310.00247longerK562AQR
A3SSRBBP5ENSG00000117222.9chr1-2050729842050731812050729842050731472050741552050742960.131000.000000.00000longerK562AQR
A3SSRBBP5ENSG00000117222.9chr1-2050681162050682862050681162050682342050688682050689400.311000.000000.00000longerK562AQR
A3SSRBBP5ENSG00000117222.9chr1-205070727205070880205070727205070790205072984205073181-0.070000.004490.02628shorterK562AQR
A5SSRBBP5ENSG00000117222.9chr1-205064000205064192205064114205064192205055269205057943-0.356000.000020.00458shorterK562RBM22

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RBBP5 is not avaliable on ENCORE database.