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RBBP5 RNA-Seq in K562



RBBP5      CRISPR in K562      Control:

General Information
RBPRBBP5
Cell_LineK562
MethodCRISPR
Exp_NameRBBP5-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1RBBP5-BGKcLV42-75
Rep2RBBP5-BGKcLV42-76
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR65.3
Rep2_qPCR68.6
Rep1_WB93.4
Rep2_WB91.9
Antibody Cat#13171S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1556




Experiment Information (Status: Sequenced)
BGKcLV42-75BGKcLV42-76
idx00
TRCN#_or_BGC#BGC#0001178BGC#0001178
shRNA_or_gRNA_sequenceCAAACATGTTGTTCTGCCGGCAAACATGTTGTTCTGCCGG
PAMTGGTGG
NameRBBP5_92RBBP5_92
Sample_IDBGKcLV42-75BGKcLV42-76
transduction_Date4/23/244/23/24
daysD6D6
RBP_nameRBBP5RBBP5
qPCR_result65.368.6
Ave_qPCR66.9
RT-qPCR_primer-Fctgttcgacccatcatagca
RT-qPCR_primer-Rtgcaaatgcactccagtttt
protein_conc21092127
WB_result93.491.9
Ave_WB92.7
WB_DONE_date4/30/24
MW70kd
IP
antibody_Cat#13171Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58395840




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RBBP5Product_ID: 13171S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: RBBP5-human		 RBBP5-HEPG2-CRISPR-13171S.png<br>Caption: Western blot following CRISPR against RBBP5 in HepG2 whole cell lysate using RBBP5 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBBP5. RBBP5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13171S_1_RBBP5.png<br>Caption: IP-WB analysis of 13171S whole cell lysate using the RBBP5 specific antibody, 13171S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RBBP5-specific antibody, 13171S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RBBP5-K562-CRISPR-13171S.png<br>Caption: Western blot following CRISPR against RBBP5 in K562 whole cell lysate using RBBP5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RBBP5. RBBP5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-75BGKcLV42-76
Sample_IDBGKcLV42-75BGKcLV42-76
Sample Name
Sample NameRBBP5-BGKcLV42-75RBBP5-BGKcLV42-76
RBPRBBP5RBBP5
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1310613107




Library-Prep Information
BGKcLV42-75BGKcLV42-76
Sample #6364
Sample NameRBBP5-BGKcLV42-75RBBP5-BGKcLV42-76
Sample_Name_Alias
Index Well PositionG08H08
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp299303
Peak_Molarity93.5067.80
libSampleQC_DNA_WellDNA_Library_set73/set1/G8.pngDNA_Library_set73/set1/H8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/G8.pngRNA_Library_set73/set1/H8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-75BGKcLV42-76
RBPRBBP5RBBP5
Batch_IDBGKcLV42BGKcLV42
WB_result93.40091.900
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43324333




Sequencing Information
BGKcLV42-75BGKcLV42-76
Sample_IDBGKcLV42-75BGKcLV42-76
Sample NameRBBP5-BGKcLV42-75RBBP5-BGKcLV42-76
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads52,568,45050,652,932
total_aligned_reads49,410,95847,184,834
unique_aligned_reads45,598,62343,538,465
percent_uniqueAligned0.867410.85954
correlation_replicates0.9979290.997929
spikein_reads6,2467,516
percent_spikeins0.000120.00015
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31993200




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database