PRKRA

General Information

Gene Name       : PRKRA
Gene ID             : ENSG00000180228.12
Essential Gene : No

Functions

Localization

Domains (AlphaFold: PRKRA)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878PRKRAENSG00000180228.1243.1632.8137.98ENCSR000AEF
HepG2PRKRAENSG00000180228.1235.0850.4842.78ENCSR985KAT
K562PRKRAENSG00000180228.1231.0635.2033.13ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
PRKRA13490S1Submitted, ENCORE2BGHcLV43-43, Submitted, ENCORE2BGKcLV42-69, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
PRKRAK562CRISPRPRKRA-BGKcLV42-69,PRKRA-BGKcLV42-70NT-BGKcLV42-1,NT-BGKcLV42-287.1,91.5SequencedENCORE2
PRKRAHepG2CRISPRPRKRA-BGHcLV43-43,PRKRA-BGHcLV43-44NT-BGHcLV43-1,NT-BGHcLV43-288.6,86.9SequencedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PRKRAProduct_ID: 13490S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PRKRA-human		 PRKRA-HEPG2-CRISPR-13490S.png<br>Caption: Western blot following CRISPR against PRKRA in HepG2 whole cell lysate using PRKRA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRKRA. PRKRA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13490S_1_PRKRA.png<br>Caption: IP-WB analysis of 13490S whole cell lysate using the PRKRA specific antibody, 13490S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PRKRA-specific antibody, 13490S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PRKRA-K562-CRISPR-13490S.png<br>Caption: Western blot following CRISPR against PRKRA in K562 whole cell lysate using PRKRA specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRKRA. PRKRA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for PRKRA is not avaliable on ENCORE database.



Proteins bound to RNA
  • PRKRA RNA is bound by 16 proteins.  







eCLIP assay for PRKRA is not avaliable on ENCORE database.





16 proteins are bound to PRKRA RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr2179300871179300901-3'-UTR,CDS,intron-Distal,intron-Proximal3.567343.27569HepG2BCLAF1
chr2179308024179308098-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.676894.52024HepG2PPIG
chr2179296864179296960-3'-UTR,CDS,intron-Distal3.993815.23915K562IGF2BP1
chr2179296654179296788-3'-UTR,intron-Distal,intron-Proximal4.291138.08036K562IGF2BP1
chr2179308015179308106-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal3.385567.03584HepG2GRWD1
chr2179309106179309178-3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal5.235675.93840HepG2PRPF8
chr2179296879179296952-3'-UTR,CDS,intron-Distal3.511853.85853K562IGF2BP2
chr2179315621179315677-5'-UTR,intron-Distal,intron-Proximal4.995407.37772HepG2EFTUD2
chr2179312200179312252-5'-UTR,CDS,intron-Distal,intron-Proximal5.218906.35567HepG2PRPF8
chr2179314935179314979-5'-UTR,CDS,intron-Distal,intron-Proximal4.556304.68690HepG2PRPF8
chr2179315629179315715-5'-UTR,CDS,intron-Distal,intron-Proximal3.310307.96871HepG2PRPF8
chr2179315646179315696-5'-UTR,CDS,intron-Distal,intron-Proximal4.378834.59919HepG2AQR
chr2179315441179315506-5'-UTR,intron-Distal,intron-Proximal4.717544.33458HepG2XPO5
chr2179314968179315074-5'-UTR,CDS,intron-Distal,intron-Proximal3.072178.44537K562YBX3
chr2179315820179315865-5'-UTR,intron-Distal,intron-Proximal4.361226.15796HepG2DDX3X
chr2179315696179315794-5'-UTR,CDS,intron-Distal,intron-Proximal4.030873.72434HepG2NCBP2
chr2179315419179315505-5'-UTR,intron-Distal,intron-Proximal3.995408.19973HepG2EFTUD2
chr2179306290179306365-5'-UTR,CDS,intron-Distal,intron-Proximal3.042183.13218K562PRPF8
chr2179315794179315868-5'-UTR,intron-Distal,intron-Proximal4.168423.98935HepG2NCBP2
chr2179313713179313782-intron-Distal4.1791411.77849HepG2QKI
chr2179301208179301234-intron-Distal4.249743.66668K562KHSRP
chr2179301060179301106-intron-Distal,intron-Proximal4.972874.40745K562SF3B4
chr2179301929179302030-intron-Distal4.143944.07352K562KHSRP
chr2179310763179310826-intron-Distal3.732793.37208HepG2ILF3
chr2179301172179301208-intron-Distal3.923783.42844K562KHSRP
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for PRKRA is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for PRKRA is not available on ENCORE database.

  • 75 RBPs can affect the levels of PRKRA.  








The Knockdown/knockout RNA-Seq assay for PRKRA is not available on ENCORE database.





75 RBPs can affect the gene expression of PRKRA :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
PRKRAENSG00000180228.8814.597801280.96710-0.653106.563705.37970HepG2ASCC1
PRKRAENSG00000180228.8540.69990866.32400-0.680107.045206.14110HepG2BOP1
PRKRAENSG00000180228.8976.285801217.00550-0.318002.018301.35160HepG2CIRBP
PRKRAENSG00000180228.8711.88190870.78920-0.290702.845602.17390HepG2CKAP4
PRKRAENSG00000180228.8663.518501157.09960-0.8023015.9847014.68950HepG2CELF1
PRKRAENSG00000180228.8465.42240805.51270-0.7914015.1033013.49860HepG2DDX19B
PRKRAENSG00000180228.8949.555701273.92020-0.424002.736102.12240HepG2DDX6
PRKRAENSG00000180228.8904.813501224.67370-0.436703.219302.58230HepG2EIF2S1
PRKRAENSG00000180228.8749.835801353.41970-0.852009.335408.16930HepG2EIF4A3
PRKRAENSG00000180228.81601.026801227.468900.383204.880203.82010HepG2EIF4G1
PRKRAENSG00000180228.8615.21230939.88410-0.611403.957202.62790HepG2FUS
PRKRAENSG00000180228.8881.706101365.50960-0.631106.321605.30180HepG2FXR1
PRKRAENSG00000180228.8485.633901266.72840-1.3832022.9276021.10090HepG2G3BP1
PRKRAENSG00000180228.81066.650001344.77100-0.334302.187301.40080HepG2G3BP2
PRKRAENSG00000180228.8315.90610744.73050-1.2372015.6156013.20880HepG2GNB2L1
PRKRAENSG00000180228.8584.30140791.80130-0.438403.183202.44610HepG2HNRNPC
PRKRAENSG00000180228.8965.543301133.56000-0.231402.633501.97540HepG2HNRNPL
PRKRAENSG00000180228.8421.03820786.56780-0.9016016.6112015.22090HepG2HNRNPU
PRKRAENSG00000180228.8872.033001287.60810-0.562205.097704.20640HepG2IGF2BP2
PRKRAENSG00000180228.8780.469901295.10180-0.730706.203004.64380HepG2KHSRP
PRKRAENSG00000180228.8963.872801365.98620-0.503004.172903.36950HepG2MAGOH
PRKRAENSG00000180228.8799.037601373.63700-0.781708.088707.05650HepG2MATR3
PRKRAENSG00000180228.8609.67850810.54170-0.410803.551002.63680HepG2MSI2
PRKRAENSG00000180228.8970.521501405.99840-0.534804.449103.65470HepG2NCBP2
PRKRAENSG00000180228.8906.416701233.88240-0.445005.508704.43870HepG2NOL12
PRKRAENSG00000180228.8838.296401228.17830-0.551009.199208.19700HepG2PCBP1
PRKRAENSG00000180228.8919.925101249.68280-0.442006.054805.30770HepG2PTBP1
PRKRAENSG00000180228.8990.955701250.22630-0.335304.950804.20460HepG2PUM1
PRKRAENSG00000180228.8665.558101249.34970-0.9085012.8017011.41960HepG2PUM2
PRKRAENSG00000180228.8774.348201250.77990-0.6918010.484108.97380HepG2RBM15
PRKRAENSG00000180228.8964.747601228.37040-0.348503.034602.33240HepG2RBM25
PRKRAENSG00000180228.81077.981301390.80570-0.367604.598603.93360HepG2NELFE
PRKRAENSG00000180228.81042.035901295.29440-0.313903.554802.86760HepG2RECQL
PRKRAENSG00000180228.8423.20060596.97450-0.496303.693002.99310HepG2RPS19
PRKRAENSG00000180228.8754.122101301.14310-0.7869016.9823015.70830HepG2SF3B4
PRKRAENSG00000180228.8632.03250816.90260-0.370204.166603.40570HepG2SMN1
PRKRAENSG00000180228.81007.846801162.83390-0.206401.884601.35440HepG2SRSF5
PRKRAENSG00000180228.8733.195301104.85380-0.5916010.423609.47120HepG2SRSF9
PRKRAENSG00000180228.8530.07960741.06140-0.483402.823301.71020HepG2STIP1
PRKRAENSG00000180228.81097.008301322.52290-0.269703.323702.65400HepG2SUCLG1
PRKRAENSG00000180228.8896.095301150.56320-0.360603.250902.29670HepG2TUFM
PRKRAENSG00000180228.8965.117401217.78980-0.335502.093001.37090HepG2UBE2L3
PRKRAENSG00000180228.81894.944801164.480700.7024017.5797016.27240K562AGGF1
PRKRAENSG00000180228.81266.665501003.254700.336203.800102.00350K562AKAP1
PRKRAENSG00000180228.81789.299301279.607900.483605.458803.96750K562AKAP8
PRKRAENSG00000180228.81850.191601211.699700.610508.223907.03720K562AKAP8L
PRKRAENSG00000180228.8985.498701472.85190-0.579706.273205.44910K562AQR
PRKRAENSG00000180228.81720.026001271.373800.435906.227304.78870K562ATP5C1
PRKRAENSG00000180228.81681.539701391.602600.272902.546201.42570K562CCDC124
PRKRAENSG00000180228.81326.917501016.592800.384203.758402.51940K562DDX3X
PRKRAENSG00000180228.81868.885201400.244900.416406.889105.92550K562DDX47
PRKRAENSG00000180228.81833.852501303.845400.492008.269706.60770K562DKC1
PRKRAENSG00000180228.8734.235701062.10930-0.532605.260704.12910K562EIF4A3
PRKRAENSG00000180228.81467.97860942.231400.639607.807106.30960K562FIP1L1
PRKRAENSG00000180228.81174.67130803.113200.548504.737803.19610K562FKBP4
PRKRAENSG00000180228.8846.507201104.55250-0.383904.085503.37140K562FXR1
PRKRAENSG00000180228.81525.835101139.236500.421402.438001.78100K562G3BP2
PRKRAENSG00000180228.81194.18050973.770800.294302.647601.53060K562GLRX3
PRKRAENSG00000180228.8655.13430924.75480-0.497304.604503.85270K562HNRNPC
PRKRAENSG00000180228.81500.950801160.443100.371102.750401.52000K562HNRNPK
PRKRAENSG00000180228.81119.58370774.973500.530604.165003.00730K562HNRNPM
PRKRAENSG00000180228.81808.607401277.501100.501505.035703.97510K562HSPD1
PRKRAENSG00000180228.81860.322901285.899400.532706.686205.27580K562KIF1C
PRKRAENSG00000180228.8860.502001220.96190-0.504805.509004.30870K562MAGOH
PRKRAENSG00000180228.81730.838801230.935800.491605.335103.67440K562MAK16
PRKRAENSG00000180228.8800.462701218.55550-0.606307.789806.14180K562MATR3
PRKRAENSG00000180228.81386.884601009.711800.457802.919102.00990K562NAA15
PRKRAENSG00000180228.8654.66860802.69470-0.294102.075201.38150K562POLR2G
PRKRAENSG00000180228.81406.826701069.582600.395303.133002.25280K562PPIG
PRKRAENSG00000180228.82141.288501201.639200.8334013.2023012.06240K562PUS1
PRKRAENSG00000180228.81598.678301170.844900.449206.106605.25100K562RBM15
PRKRAENSG00000180228.8961.587601313.32780-0.449704.491802.44170K562RBM17
PRKRAENSG00000180228.8882.773601137.68360-0.366002.848401.99350K562RBM22
PRKRAENSG00000180228.81060.74250711.980100.575104.717303.64770K562RPS19
PRKRAENSG00000180228.81096.01520714.671300.616804.434702.73980K562RRP9
PRKRAENSG00000180228.8960.433901184.64480-0.302702.605501.77840K562SF3B4
PRKRAENSG00000180228.8676.545601162.82550-0.7814011.7266010.27650K562SRSF1
PRKRAENSG00000180228.81501.323701130.784200.408803.074301.74360K562STAU1
PRKRAENSG00000180228.8719.886501176.96750-0.709208.257206.95120K562TARDBP
PRKRAENSG00000180228.8897.798201135.28860-0.338602.794101.97200K562TIA1
PRKRAENSG00000180228.81412.709201140.522700.308702.836302.02260K562U2AF2
PRKRAENSG00000180228.81473.779801101.737700.419603.101901.82280K562UTP3
PRKRAENSG00000180228.81362.311701073.218500.344004.637803.56380K562WDR43
PRKRAENSG00000180228.82148.314501659.062000.372703.674802.65360K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for PRKRA is not available on ENCORE database.





18 RBPs can affect the splicing of PRKRA :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEPRKRAENSG00000180228.8chr2-179306336179306431179300871179301046179307993179308111-0.178000.000110.00306excludedHepG2U2AF1
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793124000.090000.000020.00039includedK562AQR
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.102000.000030.00840includedHepG2HNRNPA1
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.073000.000590.06198includedHepG2LARP4
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.076000.000550.01901includedHepG2SF3A3
SEPRKRAENSG00000180228.8chr2-179306336179306431179300871179301046179307993179308111-0.071000.000350.04678excludedHepG2SSB
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.104000.000030.00287includedHepG2SF3B4
SEPRKRAENSG00000180228.8chr2-179306336179306431179300871179301046179307993179308111-0.093000.000000.00000excludedK562U2AF2
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.147000.000000.00019includedHepG2HNRNPU
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.067000.000530.08136includedK562EIF3G
SEPRKRAENSG00000180228.8chr2-179310152179310262179309148179309227179312231179312313-0.111000.000160.05785excludedHepG2DROSHA
SEPRKRAENSG00000180228.8chr2-179314968179315138179312242179312313179315692179315748-0.066000.002520.08003excludedK562PTBP1
SEPRKRAENSG00000180228.8chr2-1793101521793102621793091481793092271793122311793123130.100000.000000.00004includedK562HNRNPC
MXEPRKRAENSG00000180228.8chr2-179312231179312313179314968179315138179309148179309227179315692179315958-0.113000.003030.083022includedHepG2TAF15
MXEPRKRAENSG00000180228.8chr2-1793063361793064311793079931793081301793008711793010461793091481793092270.099000.000000.000001includedK562U2AF2
MXEPRKRAENSG00000180228.8chr2-1793091481793092271793150921793151381793079931793081111793156921793159580.192000.000000.000271includedK562TARDBP
MXEPRKRAENSG00000180228.8chr2-1793149681793150921793150921793151381793122311793123131793156921793157480.170000.000030.017051includedK562APOBEC3C
MXEPRKRAENSG00000180228.8chr2-1793122311793123131793149681793151381793091481793092271793156921793159580.116000.003290.099061includedK562HNRNPU
MXEPRKRAENSG00000180228.8chr2-1793091481793092271793150921793151381793079931793081111793156921793159580.166000.000000.000631includedK562TIA1
MXEPRKRAENSG00000180228.8chr2-1793122311793123131793149681793151381793091481793092271793156921793159580.148000.002970.079921includedHepG2NCBP2

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for PRKRA is not avaliable on ENCORE database.