Notice: Undefined offset: 1 in /website/encore_alpha/gene/index.php on line 599
PRKRA RNA-Seq in K562



PRKRA      CRISPR in K562      Control:

General Information
RBPPRKRA
Cell_LineK562
MethodCRISPR
Exp_NamePRKRA-BGKcLV42-K562
ENCODE_series_ID
Batch_IDBGKcLV42
Pool IDPool-240715
Local_Set_Nameset73
ENCODE_KD_Exp_ID
ENCODE_CN_Exp_ID
Rep1PRKRA-BGKcLV42-69
Rep2PRKRA-BGKcLV42-70
CN1NT-BGKcLV42-1
CN2NT-BGKcLV42-2
Rep1_qPCR57.1
Rep2_qPCR61.8
Rep1_WB87.1
Rep2_WB91.5
Antibody Cat#13490S
Antibody Lot#lot # 1
Antibody DCC ID
StatusSubmitted
ProjectENCORE2
ID1554




Experiment Information (Status: Sequenced)
BGKcLV42-69BGKcLV42-70
idx00
TRCN#_or_BGC#BGC#0001173BGC#0001173
shRNA_or_gRNA_sequenceGATGTACTTGGCATTCCTTGGATGTACTTGGCATTCCTTG
PAMAGGAGG
NamePRKRA_74PRKRA_74
Sample_IDBGKcLV42-69BGKcLV42-70
transduction_Date4/23/244/23/24
daysD6D6
RBP_namePRKRAPRKRA
qPCR_result57.161.8
Ave_qPCR59.4
RT-qPCR_primer-Fttccaagcaaccaaagaacc
RT-qPCR_primer-Rtccagccatgatgaatagcc
protein_conc20832388
WB_result87.191.5
Ave_WB89.3
WB_DONE_date5/14/24
MW30kd
IP
antibody_Cat#13490Slot # 1
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID58315832




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
PRKRAProduct_ID: 13490S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: PRKRA-human		 PRKRA-HEPG2-CRISPR-13490S.png<br>Caption: Western blot following CRISPR against PRKRA in HepG2 whole cell lysate using PRKRA specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRKRA. PRKRA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_13490S_1_PRKRA.png<br>Caption: IP-WB analysis of 13490S whole cell lysate using the PRKRA specific antibody, 13490S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the PRKRA-specific antibody, 13490S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 PRKRA-K562-CRISPR-13490S.png<br>Caption: Western blot following CRISPR against PRKRA in K562 whole cell lysate using PRKRA specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against PRKRA. PRKRA protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV42-69BGKcLV42-70
Sample_IDBGKcLV42-69BGKcLV42-70
Sample Name
Sample NamePRKRA-BGKcLV42-69PRKRA-BGKcLV42-70
RBPPRKRAPRKRA
Cell_LineK562K562
Exp UID
StatusSequencedSequenced
Status_date2024-10-272024-10-27
ProjectENCORE2ENCORE2
Note
ID1310213103




Library-Prep Information
BGKcLV42-69BGKcLV42-70
Sample #5960
Sample NamePRKRA-BGKcLV42-69PRKRA-BGKcLV42-70
Sample_Name_Alias
Index Well PositionC08D08
Index_tableIDT_UniqueDualIndex_96IDT_UniqueDualIndex_96
LibPrep_date2024-06-212024-06-21
Lib_IDLib-240621Lib-240621
Tecan_Location
Tecan
Tecan_date
Size_bp310306
Peak_Molarity99.20109.00
libSampleQC_DNA_WellDNA_Library_set73/set1/C8.pngDNA_Library_set73/set1/D8.png
RIN10.010.0
libSample_RNA_WellRNA_Library_set73/set1/C8.pngRNA_Library_set73/set1/D8.png
SampleQC_methodTapeStation_2022TapeStation_2022
SampleQC_date2024-07-152024-07-15
Sample_IDBGKcLV42-69BGKcLV42-70
RBPPRKRAPRKRA
Batch_IDBGKcLV42BGKcLV42
WB_result87.10091.500
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusSendToSequenceSendToSequence
ProjectENCORE2ENCORE2
ID43284329




Sequencing Information
BGKcLV42-69BGKcLV42-70
Sample_IDBGKcLV42-69BGKcLV42-70
Sample NamePRKRA-BGKcLV42-69PRKRA-BGKcLV42-70
Pool IDPool-240715Pool-240715
LocalServer_folderset73set73
total_reads39,843,64139,105,831
total_aligned_reads37,544,48336,535,906
unique_aligned_reads34,623,25833,789,044
percent_uniqueAligned0.868980.86404
correlation_replicates0.9970690.997069
spikein_reads6501,072
percent_spikeins0.000020.00003
original_ReadLength101101
QC_StatusReadyToSubmitReadyToSubmit
ID31953196




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database