POLK

General Information

Gene Name       : POLK
Gene ID             : ENSG00000122008.15
Essential Gene : No

Functions

Localization(RBPImage: POLK)

Domains (AlphaFold: POLK)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878POLKENSG00000122008.1527.7724.9926.38ENCSR000AEF
HepG2POLKENSG00000122008.153.816.064.93ENCSR985KAT
K562POLKENSG00000122008.1511.7413.9912.87ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
POLKENCAB291XLFA301-977A1Released, ENCODE3


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
POLKK562shRNABGKLV21-43,BGKLV21-44BGKLV21-1,BGKLV21-20.0,0.0NotSatisfiedENCODE3
POLKK562shRNABGKLV28-37,BGKLV28-38BGKLV28-1,BGKLV28-20.0,0.0NotSatisfiedENCODE3
POLKK562shRNABGKLV38-55,BGKLV38-56BGKLV38-1,BGKLV38-20.0,0.0NotSatisfiedENCODE3
POLKK562shRNABGKLV38-57,BGKLV38-58BGKLV38-1,BGKLV38-20.0,9.0NotSatisfiedENCODE3
POLKK562shRNABGKLV38-59,BGKLV38-60BGKLV38-1,BGKLV38-239.5,39.3NotSatisfiedENCODE3
POLKHepG2shRNABGHLV18-41,BGHLV18-42BGHLV18-1,BGHLV18-2NotSatisfiedENCODE3
POLKHepG2shRNABGHLV20-49,BGHLV20-50BGHLV20-1,BGHLV20-20.0,0.0NotSatisfiedENCODE3
POLKHepG2shRNABGHLV30-47,BGHLV30-48BGHLV30-1,BGHLV30-2NotSatisfiedENCODE3
POLKHepG2shRNABGHLV31-47,BGHLV31-48BGHLV31-1,BGHLV31-2NotSatisfiedENCODE3
POLKHepG2shRNABGHLV37-37,BGHLV37-38BGHLV37-1,BGHLV37-20.0,0.0NotSatisfiedENCODE3
POLKK562CRISPRBGKcLV11-73,BGKcLV11-74BGKcLV11-1,BGKcLV11-20.0,0.0NotSatisfiedENCODE4
POLKK562CRISPRBGKcLV11-75,BGKcLV11-76BGKcLV11-1,BGKcLV11-20.0,0.0NotSatisfiedENCODE4
POLKHepG2CRISPRBGHcLV12-59,BGHcLV12-60BGHcLV12-1,BGHcLV12-2NO CLEAR band on the position,no more ABNotSatisfiedENCODE4
POLKHepG2CRISPRBGHcLV12-61,BGHcLV12-62BGHcLV12-1,BGHcLV12-2NO CLEAR band on the position,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  
    POLK: Click to RBP Image Database
  • Nuclei
  • Nucleoplasm
  • Mutually Exclusive from Nucleolus
  • Cytoplasm
  • Cytosolic
  • Nuclear release mitosis












    • RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
    POLKProduct_ID: A301-977A
    Lot_ID: 1
    Source: Bethyl Labs
    Target Name: POLK-human    		 K562_Bethyl_A301-977A_1_POLK.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using POLK specific antibody. Lane 1 is 1% of twenty million whole cell lysate input and lane 2 is 25% of IP enrichment using rabbit normal IgG (lanes under 'IgG'). Lane 3 is 1% of twenty million whole cell lysate input and lane 4 is 10% IP enrichment using rabbit polyclonal anti-POLK antibody (lanes under 'POLK').








    RNA Targets

    • eCLIP assay for POLK is not avaliable on ENCORE database.



    Proteins bound to RNA
    • POLK RNA is bound by 10 proteins.  







    eCLIP assay for POLK is not avaliable on ENCORE database.





    10 proteins are bound to POLK RNA:

    Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
    chr57488976574889875+3'-UTR,CDS,intron-Distal,intron-Proximal3.704304.76554HepG2BCLAF1
    chr57487721474877277+3'-UTR,CDS,intron-Proximal3.512874.09885HepG2BCLAF1
    chr57487265374872767+3'-UTR,CDS,intron-Proximal3.574596.06436HepG2BCLAF1
    chr57486962874869698+3'-UTR,CDS,intron-Proximal4.599413.89826HepG2BCLAF1
    chr57486956074869677+3'-UTR,CDS,intron-Proximal3.872203.28461K562BUD13
    chr57489211074892155+3'-UTR,CDS,intron-Distal3.422593.57857HepG2BCLAF1
    chr57487710574877214+3'-UTR,CDS4.067345.65086HepG2BCLAF1
    chr57486516174865286+3'-UTR,5'-UTR,CDS,intron-Proximal4.859826.13315HepG2BCLAF1
    chr57489236074892449+3'-UTR,CDS,intron-Distal5.204307.95223HepG2BCLAF1
    chr57487707874877254+3'-UTR,CDS3.8667510.31073K562ZNF622
    chr57484832974848387+5'-UTR,CDS3.422593.57857HepG2BCLAF1
    chr57484835874848417+5'-UTR,CDS,intron-Proximal3.978373.20027K562ZNF622
    chr57483087274830971+intron-Distal,intron-Proximal3.807475.20960K562EXOSC5
    chr57481760074817639+intron-Distal3.957253.01445K562EXOSC5
    chr57485911274859123+intron-Distal5.0919823.50717K562EXOSC5
    chr57481360774813638+intron-Distal4.164773.59909K562EXOSC5
    chr57489188274892033+intron-Distal,intron-Proximal3.423783.68839K562KHSRP
    chr57481400774814061+intron-Distal5.160738.04096K562EXOSC5
    chr57485718674857270+intron-Distal3.960466.41051K562EXOSC5
    chr57481372874813746+intron-Distal3.903793.01445K562EXOSC5
    chr57483169474831745+intron-Distal4.155043.30696K562EXOSC5
    chr57486010174860153+intron-Distal3.846053.01445K562EXOSC5
    chr57482943574829498+intron-Distal4.385064.48560K562EXOSC5
    chr57485707874857139+intron-Distal4.394493.59917K562EXOSC5
    chr57483089974830979+intron-Distal3.037333.50296HepG2SUGP2
    chr57485912374859163+intron-Distal5.3959230.07027K562EXOSC5
    chr57482968674829747+intron-Distal4.079683.03671K562HNRNPM
    chr57482966074829730+intron-Distal4.614775.44433K562EXOSC5
    chr57488492274884993+intron-Distal3.498384.92145K562HNRNPL
    chr57481560774815683+intron-Distal4.196276.10949K562EXOSC5
    chr57482521974825317+intron-Distal4.019933.98095HepG2HNRNPL
    chr57488077874880814+intron-Distal,intron-Proximal4.126365.75804K562RBM22
    chr57485896674859038+intron-Distal5.4497010.67022K562EXOSC5
    chr57485893274859043+intron-Distal4.145233.27829HepG2MATR3
    ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




    eCLIP assay for POLK is not avaliable on ENCORE database.



    Diffrential Expression

    • The Knockdown/knockout assay for POLK is not available on ENCORE database.

    • 62 RBPs can affect the levels of POLK.  








    The Knockdown/knockout RNA-Seq assay for POLK is not available on ENCORE database.





    62 RBPs can affect the gene expression of POLK :

    Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
    POLKENSG00000122008.11385.15220261.828600.556703.275902.11620HepG2AATF
    POLKENSG00000122008.11489.52700347.714900.493402.194301.63970HepG2ACO1
    POLKENSG00000122008.11220.47100333.54360-0.597303.396302.29820HepG2ADAR
    POLKENSG00000122008.11622.58260412.344400.594303.641203.04210HepG2AKAP1
    POLKENSG00000122008.11647.20780424.194700.609405.790404.72820HepG2CALR
    POLKENSG00000122008.11401.89240268.090500.584002.865001.47530HepG2CSTF2T
    POLKENSG00000122008.11351.59250450.35450-0.357202.735901.99730HepG2DAZAP1
    POLKENSG00000122008.11263.48400332.87580-0.337301.973301.40570HepG2DNAJC2
    POLKENSG00000122008.11608.09070444.243800.452803.715802.76910HepG2EIF4G1
    POLKENSG00000122008.11656.98040431.936800.604903.617502.74530HepG2FIP1L1
    POLKENSG00000122008.11312.54270188.171700.731904.664203.65970HepG2FUBP3
    POLKENSG00000122008.11194.28100339.09570-0.803505.330504.47490HepG2HNRNPA1
    POLKENSG00000122008.11596.20290449.548700.407202.299701.72010HepG2HNRNPLL
    POLKENSG00000122008.11228.00600374.67640-0.716606.529105.63090HepG2HNRNPU
    POLKENSG00000122008.11366.95530659.54500-0.845904.123403.10490HepG2LARP4
    POLKENSG00000122008.11495.34930349.643300.502503.409602.51330HepG2MSI2
    POLKENSG00000122008.11454.20400333.571000.445202.336101.66840HepG2PABPC4
    POLKENSG00000122008.11237.25340334.18350-0.494203.111102.33330HepG2PPIL4
    POLKENSG00000122008.11498.81570332.188400.586405.198404.49710HepG2PTBP1
    POLKENSG00000122008.11316.32440426.26840-0.430402.566701.99030HepG2PUF60
    POLKENSG00000122008.11476.51250359.770100.405302.495701.89690HepG2QKI
    POLKENSG00000122008.11560.29320362.074100.629802.739302.03770HepG2RAVER1
    POLKENSG00000122008.11723.84040434.412100.736509.179108.36060HepG2RBM22
    POLKENSG00000122008.11596.89020326.513800.870209.624608.42190HepG2RBM25
    POLKENSG00000122008.11243.36720361.13980-0.569402.937701.91390HepG2RBM27
    POLKENSG00000122008.11440.74730344.318900.356102.120201.57170HepG2RECQL
    POLKENSG00000122008.11349.85160181.152700.949405.827504.72930HepG2RRP9
    POLKENSG00000122008.11461.42240345.852500.415802.752002.16160HepG2SF3B4
    POLKENSG00000122008.11305.79270389.13120-0.347702.263801.67260HepG2SMN1
    POLKENSG00000122008.11705.92280490.652100.524706.069005.40600HepG2SRFBP1
    POLKENSG00000122008.11588.48810360.994500.704907.648006.95340HepG2SRSF1
    POLKENSG00000122008.11272.14610370.39670-0.444702.495801.83110HepG2SRSF7
    POLKENSG00000122008.11468.25920293.687700.672907.032006.22480HepG2SRSF9
    POLKENSG00000122008.11524.97120338.343200.633703.169001.87590HepG2SUB1
    POLKENSG00000122008.11447.62830236.358200.921205.935805.00210HepG2SUPV3L1
    POLKENSG00000122008.11493.48540324.474200.604805.178904.45180HepG2TAF15
    POLKENSG00000122008.11319.65000438.13640-0.454902.453201.76510HepG2TARDBP
    POLKENSG00000122008.11492.52710298.149500.724106.909905.59560HepG2TIA1
    POLKENSG00000122008.11257.52420364.11360-0.499703.058602.53740HepG2TIAL1
    POLKENSG00000122008.11230.39000367.31970-0.673005.828305.06380HepG2TRIM56
    POLKENSG00000122008.11316.47890416.41000-0.395902.241301.46070HepG2TUFM
    POLKENSG00000122008.11516.72630369.529100.483602.799802.16810HepG2U2AF1
    POLKENSG00000122008.11578.07680355.894800.699703.837302.34380HepG2XRCC5
    POLKENSG00000122008.11157.81240232.66900-0.560102.209901.55570HepG2XRN2
    POLKENSG00000122008.11487.36380311.205800.647006.848005.86800HepG2ZC3H8
    POLKENSG00000122008.11912.54670610.783500.579105.849004.06400K562AARS
    POLKENSG00000122008.111127.185901375.36350-0.287102.443601.35130K562CCDC124
    POLKENSG00000122008.111057.350301378.44010-0.382604.664303.20260K562CPEB4
    POLKENSG00000122008.11767.42060984.98820-0.360102.838602.04590K562CPSF6
    POLKENSG00000122008.11517.07390782.35370-0.597507.939305.85850K562EIF2S1
    POLKENSG00000122008.11712.87750985.79870-0.467604.005402.97230K562FIP1L1
    POLKENSG00000122008.11593.81620773.86160-0.382103.373302.71410K562FXR1
    POLKENSG00000122008.111221.05560828.188400.560002.655301.96620K562G3BP2
    POLKENSG00000122008.11724.362101014.80120-0.486403.061502.26890K562GRSF1
    POLKENSG00000122008.11807.072801288.90710-0.675409.282807.26130K562HNRNPL
    POLKENSG00000122008.11821.139201032.30840-0.330202.398001.61300K562MAGOH
    POLKENSG00000122008.11700.68170917.93590-0.389602.992302.07940K562METAP2
    POLKENSG00000122008.11594.49060936.87040-0.656202.501501.57310K562PABPN1
    POLKENSG00000122008.11818.577601119.09100-0.451103.827202.84010K562PPIG
    POLKENSG00000122008.111338.492101697.05150-0.342402.552501.76980K562PUF60
    POLKENSG00000122008.111411.379501768.62890-0.325503.425102.35540K562PUM2
    POLKENSG00000122008.11726.37220961.92730-0.405203.185802.27780K562RBM22
    POLKENSG00000122008.11769.11990983.14320-0.354202.661001.96650K562SRSF1
    POLKENSG00000122008.11614.98100844.72130-0.457903.751402.49710K562SRSF4
    POLKENSG00000122008.11577.34000995.11510-0.785409.318207.91120K562TARDBP
    POLKENSG00000122008.11669.95920959.87200-0.518805.001003.85010K562TIA1
    POLKENSG00000122008.11725.24630941.65940-0.376702.541001.50320K562TRA2A
    POLKENSG00000122008.111031.81310829.160800.315402.578001.80150K562U2AF2
    Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".






    The Knockdown/knockout RNA-Seq assay for POLK is not available on ENCORE database.





    14 RBPs can affect the splicing of POLK :

    Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
    SEPOLKENSG00000122008.11chr5+748428347484298274807715748077397484829674848416-0.303000.000080.00249excludedK562SRSF1
    SEPOLKENSG00000122008.11chr5+748428347484298274807715748077397484829674848416-0.195000.000680.01531excludedK562SF3B4
    SEPOLKENSG00000122008.11chr5+7487638074876422748726047487275874877033748772730.340000.000120.00338includedHepG2U2AF1
    SEPOLKENSG00000122008.11chr5+7486450174864615748482967484841674865164748653170.053000.000320.07588includedK562PUM1
    SEPOLKENSG00000122008.11chr5+748828507488288374880584748807517489204674893003-0.087000.009750.07955excludedK562AQR
    SEPOLKENSG00000122008.11chr5+748828507488288374880584748807517489204674893003-0.185000.003620.04852excludedK562PUS1
    SEPOLKENSG00000122008.11chr5+7487638074876422748726047487275874877033748772730.222000.000190.00570includedK562U2AF1
    SEPOLKENSG00000122008.11chr5+7486450174864615748482967484841674865164748653170.066000.000150.08381includedK562PUM2
    SEPOLKENSG00000122008.11chr5+7487638074876422748726047487275874877033748772730.183000.000510.02234includedK562TARDBP
    SEPOLKENSG00000122008.11chr5+748897027488987474886168748862657489204674893003-0.301000.000240.01233excludedK562HNRNPU
    SEPOLKENSG00000122008.11chr5+748428347484298274807715748077397484829674848416-0.233000.000170.01171excludedK562HNRNPK
    SEPOLKENSG00000122008.11chr5+7487638074876422748726047487275874877033748772730.179000.000020.00208includedK562PTBP1
    A3SSPOLKENSG00000122008.11chr5+7486952374869694748695627486969474865164748653170.227000.000010.00010longerK562AQR
    A3SSPOLKENSG00000122008.11chr5+7487907074879242748791177487924274877033748772730.130000.000090.00078longerK562AQR
    RIPOLKENSG00000122008.11chr5+7489357174893865748935717489361474893758748938650.120000.002580.01751includedK562PUS1
    RIPOLKENSG00000122008.11chr5+7489357174893865748935717489361474893758748938650.101000.000700.01017includedK562AGGF1
    RIPOLKENSG00000122008.11chr5+7489357174893865748935717489361474893758748938650.156000.000020.00045includedK562AKAP8L
    RIPOLKENSG00000122008.11chr5+7489357174893865748935717489361474893758748938650.081000.005800.05814includedK562UTP18
    RIPOLKENSG00000122008.11chr5+7489357174893865748935717489361474893758748938650.065000.007890.04367includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74886168748862657488970274889874748828507488288374892046748930030.221000.005440.077031includedHepG2U2AF1
    MXEPOLKENSG00000122008.11chr5+74886168748862657489204674893003748828507488288374893571748936140.222000.001900.053601includedK562PTBP1
    MXEPOLKENSG00000122008.11chr5+7488285074882883748897027488987474880584748807517489204674893003-0.202000.005520.074752includedK562PUS1
    MXEPOLKENSG00000122008.11chr5+74879070748792427488058474880751748770337487727374882850748828830.168000.000030.000351includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74879070748792427488970274889874748770337487727374892046748930030.146000.000070.000901includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74882813748828837488970274889874748805847488075174892046748930030.081000.014530.085411includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74882829748828837488970274889874748805847488075174892046748930030.111000.004860.035051includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74879100748792427488970274889874748770337487727374892046748930030.054000.014640.085981includedK562AQR
    MXEPOLKENSG00000122008.11chr5+7488616874886265748920467489300374882850748828837489357174893614-0.162000.001280.011342includedK562AQR
    MXEPOLKENSG00000122008.11chr5+7488616874886265748897027488987474882850748828837489204674893003-0.166000.007510.050182includedK562AQR
    MXEPOLKENSG00000122008.11chr5+74879117748792427489204674893003748770337487727374893571748936140.154000.002540.079981includedK562AKAP8L
    MXEPOLKENSG00000122008.11chr5+74886168748862657489204674893003748828507488288374893571748936140.143000.003140.090371includedK562AKAP8L

    *: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
    **: "-log(0)" was displayed as "99999.99999".





    The Knockdown/knockout RNA-Seq assay for POLK is not avaliable on ENCORE database.