CDC5L

General Information

Gene Name       : CDC5L
Gene ID             : ENSG00000096401.7
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CDC5L)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CDC5LENSG00000096401.718.2416.2217.23ENCSR000AEF
HepG2CDC5LENSG00000096401.713.6725.6119.64ENCSR985KAT
K562CDC5LENSG00000096401.721.2622.8122.04ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CDC5L-BGKcLV28-K562ENCSR544ZMXCDC5LK562ReleasedENCSR220DTX   CRISPRKD% = 55.5,59.1






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CDC5LENCAB386CPVA301-681A1Released, ENCODE4BGKcLV28-11, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CDC5LK562CRISPRENCSR544ZMXENCSR220DTXCDC5L-BGKcLV28-11,CDC5L-BGKcLV28-12NT-BGKcLV28-1,NT-BGKcLV28-255.5,59.1ReleasedENCODE4
CDC5LHepG2CRISPRBGHcLV27-63,BGHcLV27-64BGHcLV27-1,BGHcLV27-2no clear band,NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CDC5LProduct_ID: A301-681A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CDC5L-human		 Bethyl_A301-681A_1_CDC5L.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CDC5L specific antibody, A301-681A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDC5L-specific antibody, A301-681A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CDC5L-K562-CRISPR-A301-681A.png<br>Caption: Western blot following CRISPR against CDC5L in K562 whole cell lysate using CDC5L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDC5L. CDC5L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CDC5L is not avaliable on ENCORE database.



Proteins bound to RNA
  • CDC5L RNA is bound by 23 proteins.  







eCLIP assay for CDC5L is not avaliable on ENCORE database.





23 proteins are bound to CDC5L RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr64441439444414450+3'-UTR,CDS4.064427.28307HepG2BCLAF1
chr64441569744415794+3'-UTR3.211664.61367K562UPF1
chr64441778944417895+3'-UTR5.108386.99975K562UPF1
chr64441708644417189+3'-UTR4.141405.40760HepG2UPF1
chr64435550044355570+5'-UTR,CDS3.744496.46787HepG2GRWD1
chr64435545644355564+5'-UTR,CDS3.239635.39602K562DDX3X
chr64437618244376250+CDS4.626696.01390HepG2GRWD1
chr64439047044390546+CDS3.772818.66838HepG2BCLAF1
chr64437406044374092+CDS4.144587.16955K562UCHL5
chr64441344044413518+CDS3.250214.93055K562ZNF800
chr64439745944397515+CDS5.5541010.12910HepG2BCLAF1
chr64436040544360492+CDS4.432123.71264HepG2FXR2
chr64436114944361230+CDS,intron-Proximal3.039797.53999HepG2AQR
chr64436118244361230+CDS,intron-Proximal4.489954.69071HepG2GRWD1
chr64441434244414372+CDS,intron-Proximal3.567926.09767K562UCHL5
chr64435559244355605+CDS4.565524.95438K562YBX3
chr64436040344360497+CDS,intron-Proximal4.321217.91821HepG2GRWD1
chr64436045744360527+CDS5.1242622.84851K562UCHL5
chr64435556844355591+CDS4.472047.60776K562ZNF622
chr64439049644390546+CDS3.232863.17744HepG2GRWD1
chr64435803744358102+CDS4.9901614.74774K562UCHL5
chr64435802344358100+CDS4.118726.15459HepG2GRWD1
chr64438723844387334+CDS3.847368.94096K562ZNF622
chr64437408844374145+CDS3.942179.36792K562ZNF622
chr64436408344364145+CDS4.878416.94886HepG2GRWD1
chr64437161644371674+CDS5.173847.04013HepG2BCLAF1
chr64436041444360490+CDS4.025303.02253K562MTPAP
chr64436408744364170+CDS3.055937.44851K562UCHL5
chr64437154644371603+CDS3.113497.96329K562UCHL5
chr64441344144413501+CDS4.672955.44291K562LIN28B
chr64436041344360497+CDS4.1172211.91109HepG2BCLAF1
chr64441434344414369+CDS,intron-Proximal4.215044.45558HepG2GRWD1
chr64439753544397608+CDS4.4127212.88094HepG2BCLAF1
chr64439229644392320+CDS3.960613.34184HepG2GRWD1
chr64439746444397534+CDS3.582723.60157HepG2GRWD1
chr64437166044371750+CDS5.155558.32768HepG2GRWD1
chr64441440044414446+CDS3.264316.54511K562UCHL5
chr64437405944374127+CDS,intron-Proximal3.180874.33822HepG2FXR2
chr64441359844413604+CDS4.872826.37937HepG2BCLAF1
chr64441358544413598+CDS4.996786.37937HepG2BCLAF1
chr64437405944374084+CDS,intron-Proximal3.935013.95682K562PUM1
chr64435803444358109+CDS,intron-Proximal3.624754.40430HepG2AQR
chr64439431344394439+CDS4.1988612.52201HepG2BCLAF1
chr64435802244358088+CDS4.947368.35965K562GRWD1
chr64436121344361288+CDS5.6118410.24913HepG2BCLAF1
chr64439388044393914+CDS3.513066.18248K562UCHL5
chr64439051244390528+CDS3.161093.12941K562UCHL5
chr64435559144355605+CDS3.649685.83654K562ZNF622
chr64437154444371591+CDS,intron-Proximal4.134605.94293HepG2BCLAF1
chr64439223744392271+CDS3.754074.76554HepG2BCLAF1
chr64439229544392320+CDS3.355435.17903K562UCHL5
chr64436049744360560+CDS4.415514.69071HepG2GRWD1
chr64438723744387311+CDS3.337605.96568HepG2GRWD1
chr64441434144414399+CDS,intron-Proximal3.894978.62995K562ZNF800
chr64436055444360561+CDS3.294783.73423K562UCHL5
chr64435558244355604+CDS3.138083.94979HepG2BCLAF1
chr64437416644374203+CDS4.938446.58593HepG2BCLAF1
chr64439037844390432+CDS,intron-Proximal3.543247.14018K562BUD13
chr64437172844371764+CDS3.404325.91405HepG2BCLAF1
chr64441357644413604+CDS3.138324.34780K562ZNF622
chr64437406044374075+CDS4.491344.34038HepG2BCLAF1
chr64436040344360457+CDS,intron-Proximal5.0751021.38144K562UCHL5
chr64439757044397608+CDS3.086453.01994K562BUD13
chr64441346044413529+CDS3.280865.28657K562RBM15
chr64441357044413605+CDS,intron-Proximal4.238704.69889HepG2GRWD1
chr64436118644361213+CDS5.012877.04013HepG2BCLAF1
chr64441339244413422+CDS3.039303.41227K562UCHL5
chr64441434244414363+CDS,intron-Proximal5.102546.71715HepG2BCLAF1
chr64436047844360540+CDS3.263537.87286K562ZNF622
chr64436048044360533+CDS3.939333.65750K562YBX3
chr64439036044390426+CDS,intron-Proximal4.986076.04760K562AQR
chr64441345544413505+CDS4.9320312.55461K562UCHL5
chr64437167144371731+CDS3.237476.73886K562ZNF622
chr64441345844413503+CDS4.630119.30195HepG2BCLAF1
chr64439227844392320+CDS5.194756.37937HepG2BCLAF1
chr64437410444374166+CDS5.5803410.12910HepG2BCLAF1
chr64436118344361239+CDS3.498835.20121K562GRWD1
chr64435800444358022+CDS,intron-Proximal3.324134.58459K562GRWD1
chr64435802344358103+CDS4.6563413.32408HepG2BCLAF1
chr64439430244394380+CDS3.944453.48187K562RBM15
chr64437416344374206+CDS,intron-Proximal4.426434.72426K562LIN28B
chr64437409244374200+CDS3.4613814.65559K562UCHL5
chr64439038444390415+CDS3.457336.14048K562ZNF622
chr64441439744414433+CDS3.531835.04943K562ZNF622
chr64441345344413499+CDS3.587048.82426K562ZNF622
chr64439435344394406+CDS3.568746.77066K562UCHL5
chr64439383944393901+CDS,intron-Proximal5.348817.73487HepG2BCLAF1
chr64441439544414440+CDS4.030313.12746HepG2GRWD1
chr64436118344361228+CDS3.683587.45042K562UCHL5
chr64436129244361307+CDS4.475844.55944K562ZNF622
chr64439424044394313+CDS5.4985910.12910HepG2BCLAF1
chr64438719044387213+CDS3.205794.92880K562ZNF622
chr64436040544360478+CDS4.9083819.67376K562ZNF622
chr64435556244355592+CDS4.699265.79022K562YBX3
chr64437417344374204+CDS4.043143.34184HepG2GRWD1
chr64441338944413441+CDS,intron-Proximal3.448103.68457K562LIN28B
chr64439384044393869+CDS3.664347.45042K562UCHL5
chr64437172044371765+CDS,intron-Proximal3.049945.86433K562UCHL5
chr64436123944361305+CDS3.494494.88141HepG2GRWD1
chr64437173244371765+CDS,intron-Proximal3.211375.97026K562PUM1
chr64435800344358095+CDS,intron-Proximal4.2464317.56831K562ZNF622
chr64436039744360524+CDS,intron-Proximal4.393314.02380K562TRA2A
chr64441358244413606+CDS,intron-Proximal3.126913.96055K562ZNF800
chr64437162944371691+CDS3.608055.76403K562UCHL5
chr64439215444392235+CDS,intron-Proximal3.1690010.43139K562UCHL5
chr64439513244395188+intron-Distal3.908865.18845HepG2U2AF2
chr64435563844355814+intron-Distal,intron-Proximal5.746657.66714HepG2EFTUD2
chr64441084544410900+intron-Distal4.524603.91475K562PTBP1
chr64438373644383817+intron-Distal4.521415.16513HepG2HNRNPM
chr64438494544385037+intron-Distal3.886245.20902K562EXOSC5
chr64441104644411167+intron-Distal4.614795.01910HepG2MATR3
chr64438412744384177+intron-Distal4.593763.59909K562EXOSC5
chr64438376444383809+intron-Distal4.101884.28571K562HNRNPM
chr64438403744384098+intron-Distal4.658904.03833K562HNRNPM
chr64440189744402099+intron-Distal3.998507.90970K562KHSRP
chr64437884744378997+intron-Distal3.747864.94624HepG2MATR3
chr64439417044394216+intron-Proximal3.068533.68839K562KHSRP
chr64439233244392364+intron-Proximal5.247596.44303K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CDC5L is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CDC5L is not available on ENCORE database.

  • 77 RBPs can affect the levels of CDC5L.  








The Knockdown/knockout RNA-Seq assay for CDC5L is not available on ENCORE database.





77 RBPs can affect the gene expression of CDC5L :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CDC5LENSG00000096401.71389.924101582.37470-0.187101.847201.33230HepG2ACO1
CDC5LENSG00000096401.72753.568002036.886300.434803.310002.73530HepG2AKAP1
CDC5LENSG00000096401.71315.549202068.03820-0.652609.050208.04580HepG2BOP1
CDC5LENSG00000096401.71332.429701898.57430-0.510905.375704.44700HepG2CPSF6
CDC5LENSG00000096401.71208.206901524.15020-0.335103.769002.57600HepG2YBX3
CDC5LENSG00000096401.71815.966102286.95180-0.332706.422705.36050HepG2DAZAP1
CDC5LENSG00000096401.71192.152901526.31480-0.356504.090402.70210HepG2DDX1
CDC5LENSG00000096401.71469.679701951.28450-0.408903.142402.48020HepG2DDX3X
CDC5LENSG00000096401.71146.001901675.95460-0.548404.475203.70480HepG2DDX6
CDC5LENSG00000096401.7994.220501443.50440-0.537906.084504.66760HepG2DHX30
CDC5LENSG00000096401.71054.546601496.65510-0.505107.757506.62850HepG2DNAJC21
CDC5LENSG00000096401.71012.681801483.36090-0.5507012.2064010.93990HepG2DNAJC2
CDC5LENSG00000096401.71088.773401611.37320-0.565605.369904.56090HepG2EIF2S1
CDC5LENSG00000096401.71290.738602256.10490-0.8056024.0694022.11800HepG2EIF4G1
CDC5LENSG00000096401.71480.991102161.22450-0.545306.477805.25610HepG2EIF4G2
CDC5LENSG00000096401.72297.301602648.48980-0.205201.855601.34260HepG2ETF1
CDC5LENSG00000096401.71299.679501666.67900-0.358802.352701.73610HepG2G3BP1
CDC5LENSG00000096401.71630.815902152.18900-0.400202.987302.32570HepG2GPKOW
CDC5LENSG00000096401.71253.450001680.67390-0.423104.107703.27530HepG2HNRNPC
CDC5LENSG00000096401.71482.479601933.31750-0.383105.542404.56980HepG2HNRNPF
CDC5LENSG00000096401.71657.235802083.27790-0.330106.065005.10100HepG2HNRNPL
CDC5LENSG00000096401.71662.812902053.87300-0.304702.151301.58960HepG2HNRNPLL
CDC5LENSG00000096401.71298.667601669.56420-0.362405.315104.49590HepG2HNRNPU
CDC5LENSG00000096401.71465.760801903.78230-0.377203.844103.00140HepG2IGF2BP1
CDC5LENSG00000096401.71232.950501694.20780-0.458503.844503.06060HepG2IGF2BP2
CDC5LENSG00000096401.71324.425601705.40900-0.364806.910205.80480HepG2ILF2
CDC5LENSG00000096401.71433.450401849.83380-0.367902.519301.91360HepG2NCBP2
CDC5LENSG00000096401.71766.550202267.72960-0.360304.653203.66400HepG2NOL12
CDC5LENSG00000096401.71403.396501821.14600-0.375903.124102.33060HepG2NSUN2
CDC5LENSG00000096401.71260.527301647.60870-0.386302.785802.06130HepG2PABPC4
CDC5LENSG00000096401.71222.539701489.12240-0.284602.879002.12950HepG2PPIL4
CDC5LENSG00000096401.71784.346101544.961600.207702.012001.34540HepG2PRPF4
CDC5LENSG00000096401.7844.785701199.36550-0.505602.322201.57900HepG2PRPF8
CDC5LENSG00000096401.71451.784101673.53810-0.205101.831001.36830HepG2PTBP1
CDC5LENSG00000096401.71403.734602164.70980-0.624909.484508.47150HepG2PUF60
CDC5LENSG00000096401.71755.581702297.89220-0.388408.858507.92550HepG2PUM1
CDC5LENSG00000096401.71314.263601611.76390-0.294403.872803.16790HepG2PUS1
CDC5LENSG00000096401.71404.991801812.39080-0.367304.241903.49240HepG2QKI
CDC5LENSG00000096401.71693.632102242.51190-0.405005.317204.17480HepG2RBM15
CDC5LENSG00000096401.71515.980602082.41100-0.458004.797903.93130HepG2RPLP0
CDC5LENSG00000096401.71046.887301486.83610-0.506109.698908.60630HepG2SAFB2
CDC5LENSG00000096401.71577.848502075.53130-0.395508.066206.96400HepG2SLBP
CDC5LENSG00000096401.71526.955301733.92790-0.183402.089101.51840HepG2SMN1
CDC5LENSG00000096401.73089.703801818.606800.7645027.1073025.96130HepG2SRSF1
CDC5LENSG00000096401.71869.677802137.28430-0.193002.113601.55800HepG2SRSF5
CDC5LENSG00000096401.71549.744201899.36390-0.293503.350902.59500HepG2SRSF7
CDC5LENSG00000096401.72128.128302430.72500-0.191802.449301.85670HepG2SUCLG1
CDC5LENSG00000096401.71304.558702001.87140-0.617807.805606.66830HepG2TARDBP
CDC5LENSG00000096401.71240.794801501.99410-0.275602.197901.45020HepG2TIA1
CDC5LENSG00000096401.71091.098601622.39090-0.572308.742707.94410HepG2TIAL1
CDC5LENSG00000096401.71019.056001636.78950-0.6836016.3047015.09080HepG2TRIM56
CDC5LENSG00000096401.71718.297302114.75980-0.299502.819601.93880HepG2TUFM
CDC5LENSG00000096401.72965.112401750.653700.7601011.9725010.63690HepG2U2AF1
CDC5LENSG00000096401.71661.756002238.39390-0.429803.579302.62110HepG2UBE2L3
CDC5LENSG00000096401.7677.73920914.08870-0.431602.295601.60340HepG2UCHL5
CDC5LENSG00000096401.71407.629001915.68980-0.444608.519807.40510HepG2UPF1
CDC5LENSG00000096401.71502.763401849.52540-0.299504.182803.47040HepG2UTP3
CDC5LENSG00000096401.71051.985201523.05600-0.533902.895502.15340HepG2XRN2
CDC5LENSG00000096401.71450.473502106.89410-0.5386014.8254013.80830HepG2ZNF622
CDC5LENSG00000096401.74812.467603420.171900.492606.444405.61030K562AQR
CDC5LENSG00000096401.77248.382005431.971100.416107.621606.02880K562CELF1
CDC5LENSG00000096401.74721.589403699.452800.351906.448805.51200K562DDX47
CDC5LENSG00000096401.72521.579502945.68800-0.224302.420501.73990K562EIF2S2
CDC5LENSG00000096401.72373.419701906.255900.316104.278903.55000K562FXR1
CDC5LENSG00000096401.72849.865402225.059100.357002.095901.49230K562G3BP2
CDC5LENSG00000096401.73064.053703925.65030-0.357504.070603.15580K562GRSF1
CDC5LENSG00000096401.72816.205802234.152300.333903.522002.30360K562HNRNPA1
CDC5LENSG00000096401.73129.103502474.016300.338804.590903.43590K562HNRNPU
CDC5LENSG00000096401.73647.926302892.312100.334803.568402.61800K562KIF1C
CDC5LENSG00000096401.73970.317903240.130800.293103.154902.17270K562MBNL1
CDC5LENSG00000096401.75900.285204832.630400.287902.798801.30430K562NOL12
CDC5LENSG00000096401.72785.891202278.940200.289702.453801.53610K562PCBP2
CDC5LENSG00000096401.72707.455203287.57300-0.280102.278201.53450K562PPIG
CDC5LENSG00000096401.72231.311401758.616400.343402.905002.13790K562PRPF8
CDC5LENSG00000096401.71274.471801749.56210-0.457104.407103.22610K562PSIP1
CDC5LENSG00000096401.74094.012502702.747100.599008.277907.38700K562PUS1
CDC5LENSG00000096401.73051.547202633.549400.212402.250701.70670K562RBM15
CDC5LENSG00000096401.72196.542001791.481200.294002.010801.31980K562RPS19
CDC5LENSG00000096401.71114.524401512.99020-0.441004.118802.79470K562SRFBP1
CDC5LENSG00000096401.73392.761702439.347000.475906.901005.79220K562SRSF1
CDC5LENSG00000096401.71776.994402219.07300-0.320502.501001.50780K562SRSF4
CDC5LENSG00000096401.71976.702402468.90240-0.320802.506101.82990K562TARDBP
CDC5LENSG00000096401.71982.804802381.50260-0.264302.367301.61720K562TIA1
CDC5LENSG00000096401.71464.320701787.49610-0.287702.176401.35290K562TRIM56
CDC5LENSG00000096401.73643.143102156.673600.756309.701207.47140K562U2AF1
CDC5LENSG00000096401.73595.340302227.417500.6907015.0259013.13110K562U2AF2
CDC5LENSG00000096401.73107.083902736.410800.183202.387201.59690K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CDC5L is not available on ENCORE database.





7 RBPs can affect the splicing of CDC5L :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECDC5LENSG00000096401.7chr6+443938394439392044392155443923204439421844394461-0.106000.000010.00023excludedK562SF3B4
SECDC5LENSG00000096401.7chr6+4439502944395110443942184439446144397449443975330.062000.000010.00111includedK562PRPF8
SECDC5LENSG00000096401.7chr6+443974494439764744394218443944614441339144413604-0.062000.001410.04839excludedK562PRPF8
SECDC5LENSG00000096401.7chr6+443974494439764744394267443944614441339144413604-0.058000.000000.00007excludedK562AQR
SECDC5LENSG00000096401.7chr6+443938394439392044392155443923204439744944397647-0.053000.001330.02159excludedK562PUS1
SECDC5LENSG00000096401.7chr6+443938394439392044392155443923204439421844394461-0.124000.000000.00001excludedK562U2AF1
SECDC5LENSG00000096401.7chr6+443942184439446144392155443923204439744944397647-0.078000.002070.04719excludedK562RBM15
A3SSCDC5LENSG00000096401.7chr6+4439213644392320443921554439232044390383443905460.124000.000000.00000longerK562AQR
MXECDC5LENSG00000096401.7chr6+44394218443944614439744944397647443921554439232044413391444136040.066000.000000.000011includedK562U2AF1
MXECDC5LENSG00000096401.7chr6+4439383944393920443974494439764744392155443923204441339144413604-0.087000.003090.048552includedK562PUS1
MXECDC5LENSG00000096401.7chr6+44394218443944614439744944397647443921554439232044413391444136040.103000.000000.000001includedK562AQR
MXECDC5LENSG00000096401.7chr6+44394218443944614439744944397647443921554439232044413391444136040.059000.000000.000101includedK562SF3B4
MXECDC5LENSG00000096401.7chr6+44394218443944614439744944397647443921554439232044413391444136040.059000.000550.014861includedK562PRPF8
MXECDC5LENSG00000096401.7chr6+4439421844394461443974494439764744393839443939204441339144413604-0.088000.009070.090572includedK562HNRNPUL1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CDC5L is not avaliable on ENCORE database.