CDC5L      CRISPR in HepG2      Batch: BGHcLV49

Experiment Information (Status: LibPreping)
BGHcLV49-15BGHcLV49-16
idx00
TRCN#_or_BGC#BGC#0000688BGC#0000688
shRNA_or_gRNA_sequenceGCCCGCTTGGCTAATACTCAGCCCGCTTGGCTAATACTCA
PAMGGGGGG
NameCDC5L_93CDC5L_93
Sample_IDBGHcLV49-15BGHcLV49-16
transduction_Date6/6/256/6/25
daysD6D6
RBP_nameCDC5LCDC5L
qPCR_result0.053.0
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc33653669
WB_result61.849.8
Ave_WB
WB_DONE_date6/17/25
MW92kd
IP
antibody_Cat#A301-681ALOT# 1
Antibody DCC IDENCAB386CPV
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
ActionReadyReady
Library_start_date
repeat_library
Note
ID98959896




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CDC5LProduct_ID: A301-681A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CDC5L-human		 CDC5L-hepg2-CRISPR-A301-681A-LICOR.png<br>Caption: Western blot following CRISPR against CDC5L in HepG2 whole cell lysate using CDC5L specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDC5L. CDC5L protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 Bethyl_A301-681A_1_CDC5L.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CDC5L specific antibody, A301-681A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CDC5L-specific antibody, A301-681A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 CDC5L-K562-CRISPR-A301-681A.png<br>Caption: Western blot following CRISPR against CDC5L in K562 whole cell lysate using CDC5L specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CDC5L. CDC5L protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.




Experiment Status
BGHcLV49-15BGHcLV49-16
Sample_IDBGHcLV49-15BGHcLV49-16
Sample Name
Sample Name
RBPCDC5LCDC5L
Cell_LineHepG2HepG2
Exp UID
StatusLibPrepingLibPreping
Status_date2025-08-122025-08-12
ProjectENCORE2ENCORE2
Note
ID1433414335




Library-Prep Information
BGHcLV49-15BGHcLV49-16
Sample #3132
Sample NameCDC5L-BGHcLV49-15CDC5L-BGHcLV49-16
Sample_Name_Alias
Index Well PositionA05B05
Index_tableIDT_UniqueDualIndex_96_V2IDT_UniqueDualIndex_96_V2
LibPrep_date2025-08-202025-08-20
Lib_IDLib-250820Lib-250820
Tecan_Location
Tecan
Tecan_date
Size_bp
Peak_Molarity
libSampleQC_DNA_Well
RIN
libSample_RNA_Well
SampleQC_method
SampleQC_date
Sample_IDBGHcLV49-15BGHcLV49-16
RBPCDC5LCDC5L
Batch_IDBGHcLV49BGHcLV49
WB_result61.80049.800
Library DescriptionTruSeq mRNATruSeq mRNA
Repeat_Library_Suffix
Lib_StatusIndexAssignedIndexAssigned
ProjectENCORE2ENCORE2
ID47764777




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database