YTHDF2

General Information

Gene Name       : YTHDF2
Gene ID             : ENSG00000198492.15
Essential Gene : No

Functions

Localization

Domains (AlphaFold: YTHDF2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878YTHDF2ENSG00000198492.1546.1149.1547.63ENCSR000AEF
HepG2YTHDF2ENSG00000198492.1543.1349.2346.18ENCSR985KAT
K562YTHDF2ENSG00000198492.1566.0965.7965.94ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
YTHDF271283S1Submitted, ENCORE2BGHcLV45-87, Experimented, ENCORE2BGKcLV42-107, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
YTHDF2K562CRISPRYTHDF2-BGKcLV42-107,YTHDF2-BGKcLV42-108NT-BGKcLV42-1,NT-BGKcLV42-258.2,69.2SequencedENCORE2
YTHDF2HepG2CRISPRBGHcLV45-87,BGHcLV45-88BGHcLV45-1,BGHcLV45-270.7,75.3SequencingENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
YTHDF2Product_ID: 71283S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: YTHDF2-human		 YTHDF2-HEPG2-CRISPR-71283S.png<br>Caption: Western blot following CRISPR against YTHDF2 in HepG2 whole cell lysate using YTHDF2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YTHDF2. YTHDF2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 CST_71283S_1_YTHDF2.png<br>Caption: IP-WB analysis of 71283S whole cell lysate using the YTHDF2 specific antibody, 71283S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the YTHDF2-specific antibody, 71283S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 YTHDF2-K562-CRISPR-71283S.png<br>Caption: Western blot following CRISPR against YTHDF2 in K562 whole cell lysate using YTHDF2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against YTHDF2. YTHDF2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for YTHDF2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • YTHDF2 RNA is bound by 25 proteins.  







eCLIP assay for YTHDF2 is not avaliable on ENCORE database.





25 proteins are bound to YTHDF2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr12909604729096108+3'-UTR4.140776.73573K562PUM2
chr12909595429096017+3'-UTR3.172279.61554K562PABPC4
chr12906481429064860+5'-UTR,CDS,intron-Proximal3.508717.76554K562BUD13
chr12906481629064856+5'-UTR,CDS,intron-Proximal4.364374.46921K562RBM15
chr12906365829063689+5'-UTR,CDS,intron-Proximal4.5542520.46330HepG2PRPF8
chr12906354329063663+5'-UTR,CDS,intron-Proximal4.2878515.91279K562DDX3X
chr12906353029063594+5'-UTR,intron-Proximal4.123446.63423K562GEMIN5
chr12906345729063543+5'-UTR,intron-Distal,intron-Proximal4.6391111.03053K562DDX3X
chr12906365829063663+5'-UTR,CDS,intron-Proximal3.444764.93258HepG2AQR
chr12906357129063707+5'-UTR,CDS,intron-Proximal3.1926710.35001HepG2GRWD1
chr12906365629063748+5'-UTR,CDS,intron-Proximal3.073687.86265K562PRPF8
chr12906365829063664+5'-UTR,CDS,intron-Proximal4.9777441.59844K562AQR
chr12906365029063732+5'-UTR,CDS,intron-Proximal4.019058.86977HepG2EFTUD2
chr12906899329069051+CDS4.9305911.43605K562RBM15
chr12906909129069134+CDS3.369864.68262K562RBM15
chr12906410229064187+CDS,intron-Distal,intron-Proximal3.175966.01049K562U2AF2
chr12907044129070492+CDS3.982153.34184HepG2GRWD1
chr12906419129064239+CDS,intron-Distal,intron-Proximal4.5110920.87086K562AQR
chr12906418729064237+CDS,intron-Distal,intron-Proximal3.074994.63153HepG2AQR
chr12906908929069140+CDS3.064884.13993K562IGF2BP2
chr12906899229069042+CDS3.775165.82984K562IGF2BP2
chr12906903829069130+CDS5.311079.25476K562BUD13
chr12906966729069684+CDS4.660505.01757K562IGF2BP2
chr12907010729070204+CDS3.997604.67478K562FXR1
chr12906897229069042+CDS3.971015.73713K562IGF2BP1
chr12906410929064176+CDS,intron-Distal,intron-Proximal3.7454810.38852HepG2U2AF2
chr12906412929064177+CDS,intron-Distal,intron-Proximal3.598745.73990HepG2PRPF8
chr12906968429069718+CDS3.717424.95875K562IGF2BP2
chr12906962129069656+CDS3.657824.76274K562UCHL5
chr12907012429070202+CDS3.442476.39378K562FXR2
chr12906904229069089+CDS3.215466.04547K562IGF2BP2
chr12906417729064228+CDS,intron-Distal,intron-Proximal3.498735.34140HepG2PRPF8
chr12906411429064162+intron-Distal,intron-Proximal4.6631612.08233K562AQR
chr12906423329064304+intron-Distal,intron-Proximal3.6042019.72640K562PRPF8
chr12906441829064491+intron-Distal,intron-Proximal3.704929.76099HepG2EFTUD2
chr12906412529064169+intron-Distal,intron-Proximal3.880245.23469HepG2AQR
chr12906423029064314+intron-Distal,intron-Proximal5.1956148.53254K562EFTUD2
chr12906446329064502+intron-Distal3.926269.47365K562EFTUD2
chr12906407529064128+intron-Distal,intron-Proximal3.979717.52070K562SF3B4
chr12906422829064301+intron-Distal,intron-Proximal3.126545.35747K562U2AF2
chr12906423729064296+intron-Distal,intron-Proximal3.1692912.69973HepG2BUD13
chr12906454229064610+intron-Distal3.429187.52282K562PRPF8
chr12906403329064149+intron-Distal,intron-Proximal3.393085.37650K562PTBP1
chr12906389829064030+intron-Distal3.900645.25314K562HNRNPK
chr12906424229064294+intron-Distal,intron-Proximal4.192373.43234HepG2U2AF1
chr12906441429064463+intron-Distal,intron-Proximal5.343298.21918K562EFTUD2
chr12906390429063982+intron-Distal3.229616.94036K562PTBP1
chr12906423929064293+intron-Distal,intron-Proximal4.2375715.31355K562AQR
chr12906446129064503+intron-Distal5.6607011.40920K562PRPF8
chr12906422829064295+intron-Distal,intron-Proximal4.375265.28859HepG2SF3B4
chr12906782529067890+intron-Distal3.256543.64839HepG2HNRNPC
chr12906424629064296+intron-Distal,intron-Proximal4.116375.09028HepG2U2AF2
chr12906391229064012+intron-Distal6.3932012.98213K562PCBP1
chr12906424429064304+intron-Distal,intron-Proximal4.883555.63094K562GTF2F1
chr12906423929064313+intron-Distal,intron-Proximal4.5566030.92757HepG2EFTUD2
chr12906412329064165+intron-Distal,intron-Proximal3.834666.74060K562PRPF8
chr12906401229064092+intron-Distal,intron-Proximal4.518188.88051HepG2PCBP2
chr12906390429063974+intron-Distal3.356157.43270HepG2PCBP2
chr12906424329064294+intron-Distal,intron-Proximal4.205643.85108HepG2GTF2F1
chr12906403029064152+intron-Distal,intron-Proximal4.357775.27483K562HNRNPK
chr12906422929064293+intron-Distal,intron-Proximal3.235056.49635K562SF3B4
chr12906391729064017+intron-Distal3.231376.18726HepG2U2AF1
chr12907051129070546+intron-Proximal3.836766.69182K562AQR
chr12907049829070511+intron-Proximal4.118604.49024K562AQR
chr12906367929063749+intron-Proximal3.238934.08361HepG2U2AF1
chr12906371229063735+intron-Proximal4.547206.42929HepG2RBM22
chr12906368429063723+intron-Proximal5.5066649.67790K562AQR
chr12906370529063731+intron-Proximal3.605413.63681K562RBM15
chr12906368929063710+intron-Proximal4.0602212.50237HepG2PRPF8
chr12906366429063684+intron-Proximal5.1269944.40503K562AQR
chr12906366329063712+intron-Proximal3.820916.46098HepG2AQR
chr12906368029063712+intron-Proximal3.951237.06478HepG2RBM22
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for YTHDF2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for YTHDF2 is not available on ENCORE database.

  • 70 RBPs can affect the levels of YTHDF2.  








The Knockdown/knockout RNA-Seq assay for YTHDF2 is not available on ENCORE database.





70 RBPs can affect the gene expression of YTHDF2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
YTHDF2ENSG00000198492.101646.564801335.704200.301804.138203.42310HepG2ACO1
YTHDF2ENSG00000198492.101593.606001342.122400.247702.639401.88790HepG2AKAP8L
YTHDF2ENSG00000198492.101852.426101471.451800.332102.324001.60140HepG2ASCC1
YTHDF2ENSG00000198492.101884.908701397.981500.431003.698602.81360HepG2CIRBP
YTHDF2ENSG00000198492.101624.067801335.585500.282003.922803.15750HepG2CKAP4
YTHDF2ENSG00000198492.102312.038101959.008100.238902.670502.03760HepG2CELF1
YTHDF2ENSG00000198492.101450.145602106.42660-0.5386014.1421012.68650HepG2DAZAP1
YTHDF2ENSG00000198492.10990.260601160.02360-0.228302.391601.77690HepG2DNAJC2
YTHDF2ENSG00000198492.101208.54890848.554800.510104.211103.11270HepG2EIF3D
YTHDF2ENSG00000198492.102388.185201513.683200.657805.404504.46540HepG2EIF4B
YTHDF2ENSG00000198492.101718.978202078.10890-0.273703.505602.58040HepG2EIF4G1
YTHDF2ENSG00000198492.101459.816801990.69710-0.447504.414103.40000HepG2EIF4G2
YTHDF2ENSG00000198492.101595.539201305.374000.289502.379401.60560HepG2EWSR1
YTHDF2ENSG00000198492.101704.583801318.874000.370002.344301.63140HepG2FIP1L1
YTHDF2ENSG00000198492.102089.026901544.783700.435303.847402.78710HepG2G3BP2
YTHDF2ENSG00000198492.10716.831601187.65800-0.728407.171206.17200HepG2GPKOW
YTHDF2ENSG00000198492.101432.783201726.92610-0.269402.791102.07100HepG2HNRNPF
YTHDF2ENSG00000198492.101570.884701918.79710-0.288604.567903.71980HepG2HNRNPL
YTHDF2ENSG00000198492.101151.99770909.894800.340302.638801.70790HepG2HNRNPM
YTHDF2ENSG00000198492.101355.856701700.51040-0.326803.572502.75790HepG2IGF2BP1
YTHDF2ENSG00000198492.101104.029501333.67480-0.272603.379602.57540HepG2ILF2
YTHDF2ENSG00000198492.102033.131401569.146300.373602.775002.11950HepG2MAGOH
YTHDF2ENSG00000198492.102021.392301577.924800.357202.342401.75900HepG2MATR3
YTHDF2ENSG00000198492.101407.01780888.074100.663804.587103.25260HepG2METAP2
YTHDF2ENSG00000198492.101803.298001473.673200.291103.821803.13290HepG2PCBP1
YTHDF2ENSG00000198492.10987.966101164.53300-0.237201.944201.31760HepG2PPIL4
YTHDF2ENSG00000198492.101612.699901993.87000-0.306102.677902.08950HepG2PUF60
YTHDF2ENSG00000198492.102363.017502116.577000.158801.950201.43440HepG2PUM1
YTHDF2ENSG00000198492.103284.764002133.796400.6223015.3234014.30480HepG2RBM22
YTHDF2ENSG00000198492.101900.194401668.837500.187201.821501.36260HepG2NELFE
YTHDF2ENSG00000198492.101402.380801860.60470-0.407904.335403.50960HepG2RPLP0
YTHDF2ENSG00000198492.101043.37140825.705300.337502.577401.98260HepG2RPS19
YTHDF2ENSG00000198492.10950.970401162.74570-0.290103.256702.56880HepG2SAFB2
YTHDF2ENSG00000198492.102438.838201425.699300.774407.643306.48620HepG2SF3A3
YTHDF2ENSG00000198492.102035.564501561.142300.382706.146205.32300HepG2SF3B4
YTHDF2ENSG00000198492.101577.510301911.80220-0.277304.065803.23880HepG2SLBP
YTHDF2ENSG00000198492.102917.966102410.070300.275804.604904.00660HepG2SRFBP1
YTHDF2ENSG00000198492.102606.411801629.542400.6775020.1651019.13560HepG2SRSF1
YTHDF2ENSG00000198492.101364.779701696.71300-0.314103.569402.79330HepG2SRSF7
YTHDF2ENSG00000198492.101540.911501325.760600.216901.881801.41070HepG2SRSF9
YTHDF2ENSG00000198492.101820.897102238.90310-0.298104.868904.08500HepG2SUCLG1
YTHDF2ENSG00000198492.101048.124501268.75810-0.275601.917501.48440HepG2TIAL1
YTHDF2ENSG00000198492.10979.941301280.01180-0.385405.190204.46730HepG2TRIM56
YTHDF2ENSG00000198492.101958.350401128.146200.7956011.169109.88480HepG2U2AF1
YTHDF2ENSG00000198492.101822.537901220.706700.5781012.8335011.68540HepG2UTP3
YTHDF2ENSG00000198492.102660.246401738.314600.613809.300207.14020K562AARS
YTHDF2ENSG00000198492.102696.068601897.063600.5070011.5997010.53180K562AGGF1
YTHDF2ENSG00000198492.102763.104601973.982700.485106.047105.05750K562AKAP8L
YTHDF2ENSG00000198492.109997.349103819.887801.3879046.9261044.76710K562AQR
YTHDF2ENSG00000198492.102786.533202225.775000.324102.805501.33240K562DDX19B
YTHDF2ENSG00000198492.104477.388703074.341400.5423014.8939013.49860K562DDX47
YTHDF2ENSG00000198492.102704.799502124.133800.348505.206503.95160K562DKC1
YTHDF2ENSG00000198492.102678.001501876.561200.513005.875304.72920K562EIF3G
YTHDF2ENSG00000198492.102571.434001815.686600.502006.115204.90570K562EIF4A3
YTHDF2ENSG00000198492.102935.278901888.231900.6364015.5919014.26350K562FXR1
YTHDF2ENSG00000198492.101374.382301696.11470-0.303502.393301.65260K562GPKOW
YTHDF2ENSG00000198492.102478.314201558.599000.669003.037101.74110K562HNRNPA2B1
YTHDF2ENSG00000198492.101582.978401902.42010-0.265202.093301.56460K562HNRNPC
YTHDF2ENSG00000198492.102978.160802094.881700.507506.944105.49700K562KIF1C
YTHDF2ENSG00000198492.103078.698902348.170100.390704.534803.45170K562MAGOH
YTHDF2ENSG00000198492.103069.118602346.797700.387004.640303.40020K562MBNL1
YTHDF2ENSG00000198492.102643.021201644.935900.684105.249103.89970K562NAA15
YTHDF2ENSG00000198492.105909.053304791.257300.302402.970101.42200K562NOL12
YTHDF2ENSG00000198492.102788.700302089.468200.416404.594503.32820K562PCBP2
YTHDF2ENSG00000198492.103412.372302228.712600.614508.556807.23270K562PRPF8
YTHDF2ENSG00000198492.103399.304702281.321300.575307.169406.13800K562PTBP1
YTHDF2ENSG00000198492.105867.793504779.275700.295902.893402.05890K562PUF60
YTHDF2ENSG00000198492.106311.007905083.343700.312004.250202.90450K562PUM1
YTHDF2ENSG00000198492.103384.806801957.634500.7899013.1243011.98770K562PUS1
YTHDF2ENSG00000198492.102974.988001907.432100.6412014.3188012.96250K562RBM15
YTHDF2ENSG00000198492.102920.098502302.446100.342702.844801.73470K562NELFE
YTHDF2ENSG00000198492.102296.412601246.507900.881404.202402.63550K562SF1
YTHDF2ENSG00000198492.103356.519002641.748900.345405.260304.16150K562SF3A3
YTHDF2ENSG00000198492.102635.124001826.178200.528908.804407.23090K562SF3B4
YTHDF2ENSG00000198492.102297.510301431.715300.682202.841801.59450K562SNRNP200
YTHDF2ENSG00000198492.101169.831001475.06650-0.334502.566401.58960K562SRFBP1
YTHDF2ENSG00000198492.102689.797102236.345800.266302.499201.82100K562SRSF1
YTHDF2ENSG00000198492.102545.527901903.614700.419105.671704.48790K562SYNCRIP
YTHDF2ENSG00000198492.102349.524501758.077700.418305.583404.44240K562U2AF2
YTHDF2ENSG00000198492.103080.646902525.948700.286305.033203.92140K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for YTHDF2 is not available on ENCORE database.





11 RBPs can affect the splicing of YTHDF2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029096287-0.096000.000060.00194excludedK562SRSF1
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029096287-0.068000.000020.00080excludedK562SF3B4
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029095637-0.123000.000000.00007excludedHepG2U2AF1
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029095637-0.108000.000150.00285excludedHepG2U2AF2
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029096287-0.134000.000000.00000excludedK562AQR
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029095637-0.086000.002750.05608excludedHepG2SRSF1
SEYTHDF2ENSG00000198492.10chr1+2906891429070498290647702906485029095440290956370.120000.000320.06149includedK562FXR2
SEYTHDF2ENSG00000198492.10chr1+2906891429070498290647702906485029095440290956370.069000.001280.07821includedHepG2IGF2BP3
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029095637-0.053000.000040.00958excludedK562SRSF7
SEYTHDF2ENSG00000198492.10chr1+290689142907049829064770290648502909544029095637-0.057000.001200.04683excludedK562SF3B1
MXEYTHDF2ENSG00000198492.10chr1+29064770290648462906891429070498290634612906419429095440290956370.170000.000060.002821includedHepG2MATR3
MXEYTHDF2ENSG00000198492.10chr1+2906477029064850290689142907049829063461290641942909544029095637-0.168000.000000.000142includedK562PUS1
MXEYTHDF2ENSG00000198492.10chr1+29064770290648412906891429070498290641692906419429095440290962870.068000.000010.000181includedK562AQR
MXEYTHDF2ENSG00000198492.10chr1+29064770290648502906891429070498290641692906419429095440290962860.109000.000020.000341includedK562AQR
MXEYTHDF2ENSG00000198492.10chr1+29064770290648502906891429070498290634512906365929095440290962860.115000.000000.000001includedK562AQR
MXEYTHDF2ENSG00000198492.10chr1+29064770290648502906891429069116290634512906365929095440290962870.088000.002200.018061includedK562AQR
MXEYTHDF2ENSG00000198492.10chr1+29064770290648462906891429070498290641692906419429095440290962870.065000.000040.000561includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for YTHDF2 is not avaliable on ENCORE database.