CSTF3

General Information

Gene Name       : CSTF3
Gene ID             : ENSG00000176102.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: CSTF3)


Antibody Validation

View validation images for all CSTF3 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CSTF3ENSG00000176102.1239.3433.2636.30ENCSR000AEF
HepG2CSTF3ENSG00000176102.1233.5946.3439.97ENCSR985KAT
K562CSTF3ENSG00000176102.1240.1641.0140.58ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CSTF3-BGHcLV29-HepG2ENCSR044GQUCSTF3HepG2ReleasedENCSR462MFN   CRISPRKD% = 71.5,87.4






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CSTF3ENCAB194MWLA301-094A1Released, ENCODE4BGHcLV29-47, Released, ENCODE4
CSTF3ENCAB190TLLA301-096A1Released, ENCODE4BGHcLV29-47, Released, ENCODE4BGKcLV35-17, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CSTF3K562CRISPRBGKcLV28-47,BGKcLV28-48BGKcLV28-1,BGKcLV28-237.5,38.2NotSatisfiedENCODE4
CSTF3K562CRISPRCSTF3-BGKcLV35-17,CSTF3-BGKcLV35-18NT-BGKcLV35-1,NT-BGKcLV35-254.4,50.8SubmittingENCORE2
CSTF3HepG2CRISPRENCSR044GQUENCSR462MFNCSTF3-BGHcLV29-47,CSTF3-BGHcLV29-48NT-BGHcLV29-1,NT-BGHcLV29-271.5,87.4ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CSTF3Product_ID: A301-094A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CSTF3-human
CSTF3-HEPG2-CRISPR-A301-094A.png<br>Caption: Western blot following CRISPR against CSTF3 in HepG2 whole cell lysate using CSTF3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CSTF3. CSTF3 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Bethyl_A301-094A_1_CSTF3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CSTF3 specific antibody, A301-094A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CSTF3-specific antibody, A301-094A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
CSTF3Product_ID: A301-096A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CSTF3-human
CSTF3-HEPG2-A301-096A.png<br>Caption: Western blot following CRISPR against CSTF3 in HepG2 whole cell lysate using CSTF3 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CSTF3. CSTF3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
Bethyl_A301-096A_1_CSTF3.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the CSTF3 specific antibody, A301-096A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the CSTF3-specific antibody, A301-096A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
CSTF3-K562-CRISPR-A301-096A.png<br>Caption: Western blot following CRISPR against CSTF3 in K562 whole cell lysate using CSTF3 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CSTF3. CSTF3 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CSTF3 is not avaliable on ENCORE database.



Proteins bound to RNA

  • CSTF3 RNA is bound by 22 proteins.  







eCLIP assay for CSTF3 is not avaliable on ENCORE database.





22 proteins are bound to CSTF3 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr113316341533163483-3'-UTR,CDS,intron-Distal,intron-Proximal3.677185.40231K562SRSF1
chr113316342333163466-3'-UTR,CDS,intron-Distal,intron-Proximal4.0541012.52201HepG2BCLAF1
chr113316312033163187-3'-UTR,CDS,intron-Distal,intron-Proximal3.288306.62331HepG2TIAL1
chr113316340633163476-3'-UTR,CDS,intron-Distal,intron-Proximal3.6994515.29655K562ZNF622
chr113316322733163308-3'-UTR,CDS,intron-Distal,intron-Proximal3.2870610.93472K562ZNF622
chr113316350333163534-3'-UTR,CDS,intron-Distal,intron-Proximal3.4419512.80405K562UCHL5
chr113316346633163525-3'-UTR,CDS,intron-Distal3.4654511.17783HepG2BCLAF1
chr113316349733163540-3'-UTR,CDS,intron-Distal,intron-Proximal4.495406.12255K562U2AF2
chr113316333033163401-3'-UTR,intron-Distal,intron-Proximal5.639319.62108K562SF3B4
chr113316288033162967-3'-UTR,intron-Distal,intron-Proximal3.647776.12836HepG2CSTF2
chr113316342533163480-3'-UTR,CDS,intron-Distal,intron-Proximal3.749287.35873K562GRWD1
chr113316308733163181-3'-UTR,CDS,intron-Distal,intron-Proximal4.6292111.67496K562TIA1
chr113316350433163536-3'-UTR,CDS,intron-Distal,intron-Proximal3.028483.16835K562U2AF1
chr113316301233163111-3'-UTR,intron-Distal,intron-Proximal3.425958.97082K562ZC3H11A
chr113316334233163401-3'-UTR,intron-Distal,intron-Proximal4.713676.75148K562TIA1
chr113316334433163409-3'-UTR,intron-Distal,intron-Proximal5.023955.45972HepG2TIAL1
chr113318293533183017-5'-UTR,intron-Distal,intron-Proximal3.795977.11310HepG2NCBP2
chr113318292833182961-5'-UTR,intron-Distal,intron-Proximal3.295236.52492K562SF3B4
chr113318287233182914-5'-UTR,intron-Distal5.0238828.00865K562U2AF2
chr113318284433182927-5'-UTR,CDS,intron-Distal,intron-Proximal3.0767530.96976HepG2U2AF2
chr113318290733182920-5'-UTR,intron-Distal,intron-Proximal4.8747535.21353K562U2AF1
chr113318286133182907-5'-UTR,CDS,intron-Distal4.2189538.19950K562U2AF1
chr113312121533121249-CDS,intron-Distal3.602543.68471HepG2BCLAF1
chr113318284233182859-CDS,intron-Distal,intron-Proximal3.661955.45334K562U2AF2
chr113312931133129456-CDS,intron-Distal,intron-Proximal5.074847.46464K562PRPF8
chr113312465533124699-CDS,intron-Distal4.998596.58593HepG2BCLAF1
chr113316352233163533-CDS,intron-Distal,intron-Proximal3.577363.22545HepG2PPIG
chr113312372333123790-CDS,intron-Distal4.092408.94445HepG2BCLAF1
chr113318285933182872-CDS,intron-Distal5.1949822.36512K562U2AF2
chr113312373333123834-CDS,intron-Distal4.992687.41350HepG2GRWD1
chr113311838933118440-CDS,intron-Distal3.485653.94979HepG2BCLAF1
chr113312024833120290-CDS,intron-Distal4.922596.13315HepG2BCLAF1
chr113312940733129482-CDS,intron-Distal,intron-Proximal4.107705.34978HepG2PRPF8
chr113315957733159639-intron-Distal3.029603.25653K562CSTF2T
chr113314211033142208-intron-Distal3.420895.75194HepG2CSTF2
chr113315946633159491-intron-Distal3.453137.08481K562KHSRP
chr113316187633161977-intron-Distal5.1808017.72170K562TARDBP
chr113314625933146307-intron-Distal4.089254.04290K562CSTF2T
chr113317303333173191-intron-Distal3.907443.60879K562HNRNPL
chr113312880233128871-intron-Distal3.934893.61897HepG2HNRNPC
chr113315889133158943-intron-Distal3.129613.05068HepG2U2AF2
chr113316177733161869-intron-Distal3.914185.36861HepG2U2AF2
chr113315972133159794-intron-Distal4.151844.49332HepG2CSTF2
chr113313211433132178-intron-Distal3.794803.94088HepG2QKI
chr113314829533148391-intron-Distal3.842686.48117K562KHSRP
chr113316078633160888-intron-Distal4.1971410.43497HepG2CSTF2
chr113315887933158999-intron-Distal5.4038521.66315HepG2QKI
chr113314213533142220-intron-Distal3.380746.42829K562CSTF2T
chr113316089633160955-intron-Distal3.189043.68839K562KHSRP
chr113315958933159643-intron-Distal3.298654.43403HepG2CSTF2
chr113315860133158641-intron-Distal3.088783.73266K562KHSRP
chr113317317933173283-intron-Distal3.535784.51091HepG2HNRNPL
chr113314813533148224-intron-Distal4.653427.17304K562KHSRP
chr113314222133142272-intron-Distal3.389804.13620K562KHSRP
chr113316189333161965-intron-Distal3.915306.36119HepG2KHSRP
chr113312245933122512-intron-Distal4.643826.58366HepG2SUGP2
chr113315942633159466-intron-Distal3.6940612.29548K562KHSRP
chr113314622833146302-intron-Distal4.004974.10017HepG2CSTF2
chr113312282833122896-intron-Distal4.704826.88667HepG2SUGP2
chr113317487633175015-intron-Distal3.363553.59771HepG2QKI
chr113315929833159424-intron-Distal4.6228227.60123K562KHSRP
chr113315801533158081-intron-Distal3.944444.02766HepG2HNRNPC
chr113312302033123058-intron-Distal3.920213.79098HepG2SUGP2
chr113315955433159603-intron-Distal4.270075.25174K562KHSRP
chr113315913933159207-intron-Distal4.041974.67119HepG2CSTF2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CSTF3 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CSTF3 is not available on ENCORE database.

  • 83 RBPs can affect the levels of CSTF3.  








The Knockdown/knockout RNA-Seq assay for CSTF3 is not available on ENCORE database.





83 RBPs can affect the gene expression of CSTF3 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CSTF3ENSG00000176102.71644.52760985.308700.7389019.7425018.44210HepG2ACO1
CSTF3ENSG00000176102.71290.84080989.920200.382804.965503.96500HepG2AKAP8L
CSTF3ENSG00000176102.7789.085801087.30150-0.462503.722902.10450HepG2ATP5C1
CSTF3ENSG00000176102.71246.338201608.51130-0.368005.155404.26610HepG2AUH
CSTF3ENSG00000176102.7526.06300969.56840-0.882108.644707.16930HepG2BCLAF1
CSTF3ENSG00000176102.71555.748001237.040800.330602.608302.02530HepG2BOP1
CSTF3ENSG00000176102.71441.966101138.066900.341402.531101.79170HepG2CALR
CSTF3ENSG00000176102.71307.16700689.305400.9231014.7872012.64180HepG2CSTF2
CSTF3ENSG00000176102.71310.49430804.726100.703405.623603.45850HepG2CSTF2T
CSTF3ENSG00000176102.71083.458601525.95700-0.494107.553006.58870HepG2CELF1
CSTF3ENSG00000176102.7843.535001640.73060-0.9598033.0615031.04900HepG2DAZAP1
CSTF3ENSG00000176102.7592.42130868.05600-0.551203.919403.12620HepG2DDX21
CSTF3ENSG00000176102.71459.772401130.943000.368102.204701.64000HepG2DDX3X
CSTF3ENSG00000176102.7635.93070866.75600-0.446803.655402.53520HepG2DHX30
CSTF3ENSG00000176102.71220.45980942.667900.372504.445503.62420HepG2DKC1
CSTF3ENSG00000176102.7631.60670890.79030-0.496107.240206.25500HepG2DNAJC2
CSTF3ENSG00000176102.7647.38750933.93880-0.528702.507201.94180HepG2EIF2S1
CSTF3ENSG00000176102.7523.68820703.14680-0.425102.294901.46470HepG2EIF3D
CSTF3ENSG00000176102.71751.775501004.776900.801807.286806.19930HepG2EIF4B
CSTF3ENSG00000176102.71199.643801618.72260-0.432206.562505.37320HepG2EIF4G1
CSTF3ENSG00000176102.71000.417401550.62530-0.632207.421606.11510HepG2EIF4G2
CSTF3ENSG00000176102.71314.422901846.89340-0.490708.923207.94720HepG2ETF1
CSTF3ENSG00000176102.7877.694901216.23080-0.470603.068502.26170HepG2FIP1L1
CSTF3ENSG00000176102.7977.321801276.03110-0.384804.674603.77260HepG2HNRNPF
CSTF3ENSG00000176102.71278.749901494.56550-0.225002.537001.88940HepG2HNRNPL
CSTF3ENSG00000176102.7753.977201228.54460-0.704407.677706.68890HepG2HNRNPLL
CSTF3ENSG00000176102.71253.26330839.320000.578307.488206.03980HepG2HNRNPM
CSTF3ENSG00000176102.7675.276401002.50050-0.570108.103007.10540HepG2HNRNPU
CSTF3ENSG00000176102.7948.496801256.53380-0.405703.969903.11610HepG2IGF2BP1
CSTF3ENSG00000176102.7725.695501024.01610-0.496808.207907.01130HepG2ILF2
CSTF3ENSG00000176102.7626.01720900.68240-0.524808.006506.33840HepG2KIF1C
CSTF3ENSG00000176102.71679.749601047.337800.681404.981604.15630HepG2MATR3
CSTF3ENSG00000176102.7667.11580984.61230-0.561605.401404.30200HepG2MSI2
CSTF3ENSG00000176102.7543.69860826.71700-0.604605.823704.07070HepG2NAA15
CSTF3ENSG00000176102.71582.337701072.063700.561605.135704.28460HepG2NCBP2
CSTF3ENSG00000176102.71243.063201626.94770-0.388304.522703.54970HepG2NOL12
CSTF3ENSG00000176102.7703.549401089.34990-0.630706.298005.19620HepG2NSUN2
CSTF3ENSG00000176102.7680.74230954.99640-0.488402.147901.50580HepG2PABPC4
CSTF3ENSG00000176102.71179.64270943.941400.321503.618902.94440HepG2PCBP1
CSTF3ENSG00000176102.7569.41600894.14530-0.651009.161107.86640HepG2PPIL4
CSTF3ENSG00000176102.7654.63270974.25690-0.573608.559607.30380HepG2PRPF4
CSTF3ENSG00000176102.7849.620701553.07710-0.8702014.9609013.72400HepG2PUF60
CSTF3ENSG00000176102.7818.969001003.59770-0.293302.995402.36120HepG2PUS1
CSTF3ENSG00000176102.71148.195301543.63880-0.427005.862005.17760HepG2RBM22
CSTF3ENSG00000176102.7720.46900944.08110-0.390003.136602.42340HepG2RBM25
CSTF3ENSG00000176102.7724.85250976.88310-0.430502.284701.32320HepG2RCC2
CSTF3ENSG00000176102.71246.80920995.621500.324503.637802.94370HepG2RECQL
CSTF3ENSG00000176102.71128.004401374.48640-0.285101.994801.40370HepG2RPLP0
CSTF3ENSG00000176102.7861.82540684.206000.332902.342001.77110HepG2RPS19
CSTF3ENSG00000176102.7652.31200892.75390-0.452705.754204.87840HepG2SAFB2
CSTF3ENSG00000176102.71334.10040999.978900.415805.564004.77560HepG2SF3B4
CSTF3ENSG00000176102.71024.969501489.19050-0.5389010.962709.72030HepG2SLBP
CSTF3ENSG00000176102.7709.852601069.25050-0.591004.076802.89580HepG2SMNDC1
CSTF3ENSG00000176102.7977.049301308.45220-0.421402.558401.59950HepG2SRSF3
CSTF3ENSG00000176102.71147.969901533.45590-0.417706.177705.29540HepG2SRSF5
CSTF3ENSG00000176102.7931.700101253.63260-0.428205.717304.77420HepG2SRSF7
CSTF3ENSG00000176102.71372.83670849.189900.6929015.6610014.53980HepG2SRSF9
CSTF3ENSG00000176102.71255.781301513.65290-0.269403.211502.66830HepG2SSB
CSTF3ENSG00000176102.71529.555801743.94600-0.189201.958301.41840HepG2SUCLG1
CSTF3ENSG00000176102.7836.830201092.06320-0.384001.952101.36970HepG2SUPV3L1
CSTF3ENSG00000176102.71199.02480938.248900.353703.598902.97490HepG2TAF15
CSTF3ENSG00000176102.7687.103601197.48770-0.8014010.340409.07200HepG2TARDBP
CSTF3ENSG00000176102.7600.08770974.00720-0.698809.822508.98290HepG2TIAL1
CSTF3ENSG00000176102.7620.36530982.83150-0.6638011.5505010.51520HepG2TRIM56
CSTF3ENSG00000176102.7441.84710645.16630-0.546103.642502.77330HepG2UCHL5
CSTF3ENSG00000176102.7817.709101116.76540-0.449706.338605.35270HepG2UPF1
CSTF3ENSG00000176102.71582.782801153.658600.456102.642001.61080HepG2XRCC6
CSTF3ENSG00000176102.71251.69400961.498100.380405.347204.46330HepG2ZC3H8
CSTF3ENSG00000176102.7748.017301228.22920-0.7154017.3045016.22900HepG2ZNF622
CSTF3ENSG00000176102.7966.496501306.85010-0.435306.155805.36110K562AGGF1
CSTF3ENSG00000176102.71076.011701359.81430-0.337702.757802.10560K562AKAP8L
CSTF3ENSG00000176102.71179.751901852.46090-0.651007.914607.00720K562AQR
CSTF3ENSG00000176102.7339.87110491.85100-0.533203.683802.43030K562CCAR1
CSTF3ENSG00000176102.7646.72410844.46660-0.384903.238602.10430K562DDX3X
CSTF3ENSG00000176102.71502.400201754.08510-0.223402.337301.73020K562DDX47
CSTF3ENSG00000176102.71209.637601476.39730-0.287502.893202.16080K562EIF2S2
CSTF3ENSG00000176102.7273.11910500.30430-0.873304.812403.44540K562EIF3A
CSTF3ENSG00000176102.7766.607301094.97980-0.514303.560602.37180K562EIF3D
CSTF3ENSG00000176102.7760.588501214.75000-0.6755010.738709.16650K562EIF3G
CSTF3ENSG00000176102.7612.14180794.43200-0.376103.477502.15710K562EIF4B
CSTF3ENSG00000176102.71909.182202356.14450-0.303503.484002.43250K562EIF4G2
CSTF3ENSG00000176102.71025.110201276.46050-0.316402.262901.49580K562FIP1L1
CSTF3ENSG00000176102.71199.25370917.453500.386304.223503.49870K562FXR1
CSTF3ENSG00000176102.71644.010801967.57540-0.259201.963901.32110K562GRSF1
CSTF3ENSG00000176102.71435.763601049.932200.451403.441702.39380K562HNRNPM
CSTF3ENSG00000176102.71096.461701367.82880-0.319003.269702.30140K562HNRNPU
CSTF3ENSG00000176102.7670.56270960.48070-0.518403.162202.15620K562KRR1
CSTF3ENSG00000176102.71546.580501220.570300.341403.007902.13080K562MAGOH
CSTF3ENSG00000176102.7975.910201185.85430-0.281102.180701.57960K562PTBP1
CSTF3ENSG00000176102.73369.735202570.753100.390304.022503.04300K562PUF60
CSTF3ENSG00000176102.7873.541201137.13760-0.380503.046302.15920K562RBM22
CSTF3ENSG00000176102.7687.576901013.68540-0.560004.031903.04280K562RPS19
CSTF3ENSG00000176102.7481.57500686.91960-0.512402.639801.64470K562SRFBP1
CSTF3ENSG00000176102.71559.924401162.523400.424104.578803.67280K562SRSF1
CSTF3ENSG00000176102.7992.261001253.03510-0.336603.421301.97550K562SSB
CSTF3ENSG00000176102.7766.299801019.78020-0.412302.825701.79440K562UCHL5
CSTF3ENSG00000176102.71676.806202086.91720-0.315702.758601.89700K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CSTF3 is not available on ENCORE database.





2 RBPs can affect the splicing of CSTF3 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECSTF3ENSG00000176102.7chr11-331274283312746333127112331272393312754333127610-0.061000.012270.09545excludedK562AQR
SECSTF3ENSG00000176102.7chr11-3316641533166519331634303316353233182844331830410.104000.000000.00000includedK562HNRNPC
A3SSCSTF3ENSG00000176102.7chr11-3312993133130082331299313312996433163212331633080.116000.000070.00061longerK562AQR
A3SSCSTF3ENSG00000176102.7chr11-3310744033107648331074403310753533108533331087770.064000.000000.00002longerK562AQR
A3SSCSTF3ENSG00000176102.7chr11-3312057933120721331205793312069633121153331212630.230000.000260.00206longerK562AQR
A3SSCSTF3ENSG00000176102.7chr11-3311838033118572331183803311852433120235331203940.123000.000020.00018longerK562AQR
A3SSCSTF3ENSG00000176102.7chr11-3310853333108777331085333310868733112077331122730.185000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CSTF3 is not avaliable on ENCORE database.