NSUN5

General Information

Gene Name       : NSUN5
Gene ID             : ENSG00000130305.16
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: NSUN5)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878NSUN5ENSG00000130305.165.7711.178.47ENCSR000AEF
HepG2NSUN5ENSG00000130305.1620.669.1014.88ENCSR985KAT
K562NSUN5ENSG00000130305.1647.3528.9538.15ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq NSUN5-BGKcLV19-K562ENCSR982XTONSUN5K562ReleasedENCSR978MMZ   CRISPRKD% = 91.1,89.6687668






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
NSUN5ENCAB884UJQPA5-54228TK2671522AReleased, ENCODE4BGKcLV19-29, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
NSUN5K562CRISPRENCSR982XTOENCSR978MMZNSUN5-BGKcLV19-29,NSUN5-BGKcLV19-30NT-BGKcLV19-1,NT-BGKcLV19-291.1,89.6687668ReleasedENCODE4
NSUN5K562CRISPRBGKcLV19-31,BGKcLV19-32BGKcLV19-1,BGKcLV19-20.0,0.0NotSatisfiedENCODE4
NSUN5HepG2CRISPRBGHcLV20-15,BGHcLV20-16BGHcLV20-1,BGHcLV20-20.0,0.0NotSatisfiedENCODE4
NSUN5HepG2CRISPRBGHcLV24-45,BGHcLV24-46BGHcLV24-1,BGHcLV24-20.0,0.0NotSatisfiedENCODE4
NSUN5HepG2CRISPRBGHcLV25-47,BGHcLV25-48BGHcLV25-1,BGHcLV25-20.0,0.0NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
NSUN5Product_ID: PA5-54228
Lot_ID: TK2671522A
Source: Thermo Fisher
Target Name: NSUN5-human		 ThermoFisher_PA5-54228_TK2671522A_NSUN5.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the NSUN5 specific antibody, PA5-54228. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the NSUN5-specific antibody, PA5-54228. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 NSUN5-K562-CRISPR-PA5-40919.png<br>Caption: Western blot following CRISPR against NSUN5 in K562 whole cell lysate using NSUN5 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against NSUN5. NSUN5 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for NSUN5 is not avaliable on ENCORE database.



Proteins bound to RNA
  • NSUN5 RNA is bound by 15 proteins.  







eCLIP assay for NSUN5 is not avaliable on ENCORE database.





15 proteins are bound to NSUN5 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr77271725472717314-3'-UTR,intron-Distal5.5270213.81571K562PABPC4
chr77271840172718480-3'-UTR,CDS,intron-Distal,intron-Proximal4.323113.93366K562RBFOX2
chr77271837672718399-3'-UTR,CDS,intron-Distal3.662635.34337K562AQR
chr77271725472717311-3'-UTR,intron-Distal3.862373.53569K562LIN28B
chr77271724172717313-3'-UTR,intron-Distal4.1218413.85564HepG2SUB1
chr77271725172717364-3'-UTR,intron-Distal3.552504.48863K562NOLC1
chr77271833572718428-3'-UTR,CDS,intron-Distal,intron-Proximal3.366163.01994K562BUD13
chr77271754372717609-3'-UTR,CDS,intron-Distal,intron-Proximal5.400198.38577HepG2PRPF8
chr77271839972718430-3'-UTR,CDS,intron-Distal,intron-Proximal5.358797.63570K562AQR
chr77272269572722800-5'-UTR,CDS,intron-Distal3.3118610.97253K562DDX3X
chr77272270372722795-5'-UTR,CDS,intron-Distal4.279624.36077HepG2NCBP2
chr77272145472721548-CDS,intron-Distal,intron-Proximal3.059686.82062K562PRPF8
chr77272243772722521-CDS,intron-Distal4.431887.13477K562DDX3X
chr77271788272717937-CDS,intron-Distal,intron-Proximal5.224265.18845K562PCBP1
chr77272172472721777-CDS,intron-Distal,intron-Proximal3.458945.59104K562BUD13
chr77272164672721734-CDS,intron-Distal,intron-Proximal3.403674.56666K562HNRNPM
chr77272147072721538-CDS,intron-Distal,intron-Proximal3.968186.10859HepG2EFTUD2
chr77272165972721727-CDS,intron-Distal,intron-Proximal4.117285.64813K562DDX24
chr77272234772722430-CDS,intron-Distal,intron-Proximal4.9110811.42662K562AQR
chr77272148572721540-CDS,intron-Distal,intron-Proximal4.152723.93512K562SMNDC1
chr77271819372718229-CDS,intron-Distal,intron-Proximal3.992114.98250K562AQR
chr77272165372721750-CDS,intron-Distal,intron-Proximal5.477146.82887HepG2EFTUD2
chr77272150972721620-CDS,intron-Distal,intron-Proximal3.582068.43093HepG2PRPF8
chr77271777372717840-CDS,intron-Distal,intron-Proximal3.348783.71591HepG2PRPF8
chr77272166472721724-CDS,intron-Distal,intron-Proximal4.711235.80866K562BUD13
chr77271866572718735-intron-Distal,intron-Proximal3.474713.76553K562PRPF8
chr77271869872718744-intron-Distal,intron-Proximal6.001078.88118K562AQR
chr77272256172722609-intron-Distal,intron-Proximal5.235734.28841HepG2BUD13
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for NSUN5 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for NSUN5 is not available on ENCORE database.

  • 72 RBPs can affect the levels of NSUN5.  








The Knockdown/knockout RNA-Seq assay for NSUN5 is not available on ENCORE database.





72 RBPs can affect the gene expression of NSUN5 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
NSUN5ENSG00000130305.12390.14600308.580900.338302.074501.48660HepG2CKAP4
NSUN5ENSG00000130305.12463.86750292.877900.663306.504905.59820HepG2CELF1
NSUN5ENSG00000130305.12532.11080316.270700.750504.742703.94150HepG2DDX3X
NSUN5ENSG00000130305.12477.90490271.844300.813802.528001.93630HepG2DDX6
NSUN5ENSG00000130305.12214.65290138.859400.628302.998202.32420HepG2DNAJC2
NSUN5ENSG00000130305.12395.68630301.607100.391602.121201.39230HepG2EWSR1
NSUN5ENSG00000130305.12446.15980291.365700.614602.834202.13320HepG2FXR1
NSUN5ENSG00000130305.12314.09830191.715800.712102.927701.51690HepG2HNRNPA0
NSUN5ENSG00000130305.12512.03310273.850300.902706.111805.10300HepG2HNRNPK
NSUN5ENSG00000130305.12211.75810156.273900.438201.989701.46600HepG2HNRNPU
NSUN5ENSG00000130305.12263.01180169.451200.634202.483501.41320HepG2LARP7
NSUN5ENSG00000130305.12473.34880291.468500.699504.672703.82660HepG2MAGOH
NSUN5ENSG00000130305.12487.07060293.061700.732804.676003.87300HepG2MATR3
NSUN5ENSG00000130305.12518.52270299.934000.789705.804504.90260HepG2NCBP2
NSUN5ENSG00000130305.12359.80930269.900100.414702.460101.88030HepG2PCBP1
NSUN5ENSG00000130305.12365.17750274.632300.411002.336401.82620HepG2PTBP1
NSUN5ENSG00000130305.12458.94130298.070900.622604.736503.98220HepG2PUF60
NSUN5ENSG00000130305.12492.72500316.424800.638807.308306.43940HepG2PUM1
NSUN5ENSG00000130305.12681.57540464.974400.551602.529702.02830HepG2RBM22
NSUN5ENSG00000130305.12340.05700204.341300.734703.748602.58320HepG2RBM27
NSUN5ENSG00000130305.12269.02180165.935200.697002.944601.84840HepG2RCC2
NSUN5ENSG00000130305.12397.67040305.640300.379602.035801.55660HepG2NELFE
NSUN5ENSG00000130305.12209.32830116.769200.842002.108101.56430HepG2RPS19
NSUN5ENSG00000130305.12303.21420192.374600.656302.969301.85580HepG2RPS5
NSUN5ENSG00000130305.12508.87110278.586500.869106.420205.36800HepG2SF3A3
NSUN5ENSG00000130305.12180.04670258.14270-0.519802.204901.57000HepG2SF3B1
NSUN5ENSG00000130305.12443.21770285.929700.632305.267504.49650HepG2SF3B4
NSUN5ENSG00000130305.12247.41400162.287200.608303.580302.86450HepG2SMN1
NSUN5ENSG00000130305.12301.26320160.627500.907203.343402.27960HepG2SMNDC1
NSUN5ENSG00000130305.12770.53620525.159100.553006.799006.11050HepG2SRFBP1
NSUN5ENSG00000130305.12408.93900294.312800.474403.506202.81720HepG2SRSF5
NSUN5ENSG00000130305.12371.75250455.94070-0.294501.827501.38950HepG2SSB
NSUN5ENSG00000130305.12317.53750198.081200.680703.138501.96250HepG2STIP1
NSUN5ENSG00000130305.12469.24860334.705100.487404.108903.37690HepG2SUCLG1
NSUN5ENSG00000130305.12178.94210268.25440-0.584103.141502.54920HepG2TAF15
NSUN5ENSG00000130305.12234.82930151.833300.629003.260702.72460HepG2TIAL1
NSUN5ENSG00000130305.12206.44030153.207700.430101.880201.39720HepG2TRIM56
NSUN5ENSG00000130305.12366.12820249.774000.551602.947802.30050HepG2U2AF1
NSUN5ENSG00000130305.12498.84240155.928901.677606.281304.72280HepG2U2AF2
NSUN5ENSG00000130305.12277.27950148.431500.901405.185404.13820HepG2UCHL5
NSUN5ENSG00000130305.12390.47410238.530400.711006.048205.22070HepG2UTP3
NSUN5ENSG00000130305.121569.494701015.171100.6285012.6756011.55620K562AGGF1
NSUN5ENSG00000130305.121514.893301056.324100.520105.692304.73540K562AKAP8L
NSUN5ENSG00000130305.121363.880601108.324100.299202.296501.47140K562ATP5C1
NSUN5ENSG00000130305.12841.21410618.394100.443803.355202.17420K562CSTF2
NSUN5ENSG00000130305.121961.870601206.082900.7018017.5933016.07490K562DDX47
NSUN5ENSG00000130305.121578.626001136.727300.473707.077205.56830K562DKC1
NSUN5ENSG00000130305.121109.42210635.161100.804506.018104.62840K562EFTUD2
NSUN5ENSG00000130305.12986.51530587.606300.747403.147302.06680K562EIF3A
NSUN5ENSG00000130305.12686.66890370.283700.8909012.8929011.13890K562EIF3G
NSUN5ENSG00000130305.12971.75440751.322200.371102.694801.85750K562FIP1L1
NSUN5ENSG00000130305.121324.17670945.103000.486506.784905.89730K562FXR1
NSUN5ENSG00000130305.12106.26720300.70060-1.500608.164106.82010K562G3BP2
NSUN5ENSG00000130305.122227.407001560.105800.513606.751505.56360K562GRSF1
NSUN5ENSG00000130305.12741.77140553.120700.423302.853201.63800K562IGF2BP3
NSUN5ENSG00000130305.121018.18880571.359800.8334012.1982010.34150K562MAGOH
NSUN5ENSG00000130305.121688.385301255.855600.426904.905803.62730K562MBNL1
NSUN5ENSG00000130305.121161.53730880.261500.399902.180001.41760K562NAA15
NSUN5ENSG00000130305.12784.22290508.320000.625406.096504.62720K562PCBP2
NSUN5ENSG00000130305.12798.231901073.69320-0.427702.908402.05490K562PCBP3
NSUN5ENSG00000130305.121193.58740852.883300.484804.354603.29390K562PPIG
NSUN5ENSG00000130305.121197.02910618.093200.953507.247406.03570K562PRPF8
NSUN5ENSG00000130305.121107.70230555.131700.996606.251305.28710K562PTBP1
NSUN5ENSG00000130305.121648.460302163.75930-0.392405.170703.67860K562PUM1
NSUN5ENSG00000130305.121663.636801047.637300.667108.343207.44830K562PUS1
NSUN5ENSG00000130305.121396.310901020.720300.451905.637804.81640K562RBM15
NSUN5ENSG00000130305.12871.64130532.159400.711808.042906.51110K562RBM22
NSUN5ENSG00000130305.121495.02600629.361801.248106.757505.46860K562RPS19
NSUN5ENSG00000130305.121194.36940747.065600.676802.680001.50380K562SF1
NSUN5ENSG00000130305.12570.36160312.387800.868406.684005.31800K562SF3B4
NSUN5ENSG00000130305.12207.74920340.00260-0.710703.683401.78190K562SND1
NSUN5ENSG00000130305.121069.97680544.244100.9752011.9528010.48550K562SRSF1
NSUN5ENSG00000130305.12755.09410514.071600.554604.848203.38850K562SRSF5
NSUN5ENSG00000130305.12277.10940381.96550-0.463002.588401.35650K562SSB
NSUN5ENSG00000130305.12735.22180985.79880-0.423103.262601.76760K562SSRP1
NSUN5ENSG00000130305.121164.67430550.746001.0804017.2704015.20060K562TARDBP
NSUN5ENSG00000130305.12897.79200531.273700.756808.786707.21300K562TIA1
NSUN5ENSG00000130305.12850.06440521.490100.704803.447502.20080K562TRA2A
NSUN5ENSG00000130305.121033.338701236.72470-0.259202.678101.97100K562UPF1
NSUN5ENSG00000130305.121636.898402007.06490-0.294102.642701.38510K562WRN
NSUN5ENSG00000130305.121563.468501908.43340-0.287602.316801.53870K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for NSUN5 is not available on ENCORE database.





17 RBPs can affect the splicing of NSUN5 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
A3SSNSUN5ENSG00000130305.12chr7-7271822672718405727182267271840172718743727188590.234000.000050.03420longerK562EIF3G
A3SSNSUN5ENSG00000130305.12chr7-7271792772718109727179277271803372718226727184530.074000.000000.00000longerK562AQR
A3SSNSUN5ENSG00000130305.12chr7-7271895572719185727189557271909472721390727214990.130000.000000.00000longerK562AQR
A3SSNSUN5ENSG00000130305.12chr7-7271831272718510727183127271840572718743727188590.137000.000000.00000longerK562AQR
A3SSNSUN5ENSG00000130305.12chr7-7271895572719185727189557271909472721390727214990.099000.000000.00000longerK562PUS1
A3SSNSUN5ENSG00000130305.12chr7-7271822672718405727182267271840172718743727188590.213000.000010.00278longerK562PTBP1
A3SSNSUN5ENSG00000130305.12chr7-7271895572719185727189557271909472721390727214990.075000.000890.04189longerK562U2AF1
RINSUN5ENSG00000130305.12chr7-727215797272255072721579727217547272242772722550-0.145000.000080.01176excludedK562NONO
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.064000.000750.04628includedK562DROSHA
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.244000.000000.00000includedK562PUS1
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.151000.000000.00000includedK562PUS1
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.243000.006690.04323includedHepG2U2AF2
RINSUN5ENSG00000130305.12chr7-727172267271773772717226727175267271759672717737-0.090000.000020.00032excludedHepG2U2AF2
RINSUN5ENSG00000130305.12chr7-727215797272255072721579727217547272242772722550-0.217000.000350.01062excludedK562SRSF1
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.089000.000310.00494includedK562AGGF1
RINSUN5ENSG00000130305.12chr7-727215797272255072721579727217547272242772722550-0.403000.000440.07212excludedHepG2NOL12
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.173000.000050.00295includedHepG2FTO
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.275000.002550.05259includedHepG2SRSF1
RINSUN5ENSG00000130305.12chr7-727215797272255072721579727217547272242772722550-0.121000.001920.09913excludedK562XRN2
RINSUN5ENSG00000130305.12chr7-727172267271773772717226727175267271759672717737-0.078000.000330.03416excludedK562AARS
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.134000.000000.00001includedK562AKAP8L
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.161000.008900.07206includedHepG2U2AF1
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.151000.000000.00000includedK562RBM15
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.186000.000530.00842includedK562UTP18
RINSUN5ENSG00000130305.12chr7-7271722672717737727172267271752672717596727177370.294000.000000.00000includedK562AQR
RINSUN5ENSG00000130305.12chr7-7272157972722550727215797272175472722427727225500.087000.000020.00014includedK562AQR
RINSUN5ENSG00000130305.12chr7-727178227271805172717822727179117271803372718051-0.155000.000690.00481excludedK562AQR
RINSUN5ENSG00000130305.12chr7-727215797272255072721579727217547272242772722550-0.220000.000460.01174excludedK562PTBP1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for NSUN5 is not avaliable on ENCORE database.