METTL1

General Information

Gene Name       : METTL1
Gene ID             : ENSG00000037897.16
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: METTL1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878METTL1ENSG00000037897.1618.7818.2018.49ENCSR000AEF
HepG2METTL1ENSG00000037897.1637.1331.1334.13ENCSR985KAT
K562METTL1ENSG00000037897.1626.5632.0429.30ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
eCLIPeCLIP: 4225ENCSR498DQSMETTL1K562ReleasedENCAB061GXOENCSR181YHI  
knockdown followed by RNA-seq METTL1-BGKcLV23-K562ENCSR607SRHMETTL1K562ReleasedENCSR877JEM   CRISPRKD% = 83.2,76.6
knockdown followed by RNA-seq METTL1-BGHcLV24-HepG2ENCSR925PORMETTL1HepG2ReleasedENCSR671FDZ   CRISPRKD% = 69.0,65.0






  eCLIP  
  RBP    Exp_UID    Cell_Line    ENCODE_ACCESSION    Control_ENCODE_ACCESSION    ENCODE_Antibody_ID    Antibody    Lot_num    Status    Project  
METTL14225K562ENCSR498DQSENCSR181YHIENCAB061GXOPA5-80810UL2893376ReleasedENCODE4


  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
METTL1ENCAB061GXOPA5-80810UL2893376BGHcLV24-37, Released, ENCODE4BGKcLV23-51, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
METTL1K562CRISPRBGKcLV23-49,BGKcLV23-50BGKcLV23-1,BGKcLV23-276.8,75.3NotSatisfiedENCODE4
METTL1K562CRISPRENCSR607SRHENCSR877JEMMETTL1-BGKcLV23-51,METTL1-BGKcLV23-52NT-BGKcLV23-1,NT-BGKcLV23-283.2,76.6ReleasedENCODE4
METTL1HepG2CRISPRENCSR925PORENCSR671FDZMETTL1-BGHcLV24-37,METTL1-BGHcLV24-38NT-BGHcLV24-1,NT-BGHcLV24-269.0,65.0ReleasedENCODE4
METTL1HepG2CRISPRBGHcLV24-39,BGHcLV24-40BGHcLV24-1,BGHcLV24-255.0,54.0NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
METTL1Product_ID: PA5-80810
Lot_ID: UL2893376
Source: Thermo Fisher
Target Name: METTL1-human		 METTL1-HEPG2-CRISPR-PA5-80810.png<br>Caption: Western blot following CRISPR against METTL1 in HepG2 whole cell lysate using METTL1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against METTL1. METTL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 METTL1-K562-CRISPR-PA5-80810.png<br>Caption: Western blot following CRISPR against METTL1 in K562 whole cell lysate using METTL1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against METTL1. METTL1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for METTL1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • METTL1 RNA is bound by 3 proteins.  







eCLIP assay for METTL1 is not avaliable on ENCORE database.





3 proteins are bound to METTL1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr125816330158163356-3'-UTR,CDS,intron-Distal,intron-Proximal4.5162710.27119HepG2PRPF8
chr125816279058162912-3'-UTR,CDS,intron-Distal,intron-Proximal4.173814.45558HepG2GRWD1
chr125816330158163349-3'-UTR,CDS,intron-Distal,intron-Proximal4.699633.62536K562AQR
chr125816313958163246-3'-UTR,CDS,intron-Distal,intron-Proximal4.422564.59919HepG2AQR
chr125816318558163253-3'-UTR,CDS,intron-Distal,intron-Proximal4.734824.09264K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for METTL1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for METTL1 is not available on ENCORE database.

  • 65 RBPs can affect the levels of METTL1.  








The Knockdown/knockout RNA-Seq assay for METTL1 is not available on ENCORE database.





65 RBPs can affect the gene expression of METTL1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
METTL1ENSG00000037897.12503.07170250.510201.005807.753706.91090HepG2AKAP1
METTL1ENSG00000037897.12375.07960207.284200.855505.977604.84490HepG2ASCC1
METTL1ENSG00000037897.12283.13700387.84150-0.454002.943102.23830HepG2AUH
METTL1ENSG00000037897.12363.62630250.036100.540202.545801.64940HepG2BCCIP
METTL1ENSG00000037897.12255.25840360.72640-0.498902.591202.00960HepG2BOP1
METTL1ENSG00000037897.12180.60590376.07060-1.058205.740904.35010HepG2DDX59
METTL1ENSG00000037897.12374.93570213.223000.814204.329303.48080HepG2EIF4B
METTL1ENSG00000037897.12361.87690237.242400.609003.432902.50080HepG2EWSR1
METTL1ENSG00000037897.12173.65890358.28290-1.044807.855006.85510HepG2HNRNPLL
METTL1ENSG00000037897.12249.90170345.85130-0.468803.064902.30490HepG2IGF2BP1
METTL1ENSG00000037897.12404.52590222.260500.863905.678104.79900HepG2MATR3
METTL1ENSG00000037897.12315.97140227.503800.473802.019901.47450HepG2NCBP2
METTL1ENSG00000037897.12351.76120215.270800.708305.860605.02970HepG2PCBP1
METTL1ENSG00000037897.12102.63540212.30110-1.048605.295603.89170HepG2PCBP2
METTL1ENSG00000037897.12120.10000189.67720-0.659302.268001.53370HepG2PRPF8
METTL1ENSG00000037897.12223.71650320.37060-0.518102.351601.79560HepG2PUF60
METTL1ENSG00000037897.12413.24290340.125900.280801.898501.38700HepG2PUM1
METTL1ENSG00000037897.12424.95080339.853600.322301.987501.41310HepG2PUM2
METTL1ENSG00000037897.12483.50630372.186100.377402.498801.99980HepG2RBM22
METTL1ENSG00000037897.12269.05530378.45350-0.492203.135802.41520HepG2RPLP0
METTL1ENSG00000037897.12118.34970201.29480-0.766302.989202.00950HepG2RPS10
METTL1ENSG00000037897.12211.88040151.194900.486701.872801.35830HepG2RPS19
METTL1ENSG00000037897.12291.23530179.980800.694205.242604.40550HepG2SMN1
METTL1ENSG00000037897.12215.34530394.59730-0.873704.623203.60250HepG2SNRNP70
METTL1ENSG00000037897.12312.10850238.039900.390701.947401.53110HepG2SRSF1
METTL1ENSG00000037897.12209.15640381.55900-0.867305.158603.75290HepG2SRSF3
METTL1ENSG00000037897.12241.83280345.09070-0.513003.618102.83830HepG2SRSF7
METTL1ENSG00000037897.12272.31740364.97690-0.422502.648702.14300HepG2SSB
METTL1ENSG00000037897.12474.98450359.755100.400802.867402.23680HepG2SUCLG1
METTL1ENSG00000037897.12254.31710349.15400-0.457202.142501.49270HepG2TARDBP
METTL1ENSG00000037897.12250.53890169.896000.560303.210802.61370HepG2TRIM56
METTL1ENSG00000037897.12186.75130270.29740-0.533403.162702.42680HepG2UPF1
METTL1ENSG00000037897.12193.41090269.93210-0.480902.673402.01250HepG2ZC3H8
METTL1ENSG00000037897.1288.86110173.28880-0.963604.934704.21300K562AGGF1
METTL1ENSG00000037897.12111.61350180.30990-0.692003.012002.32960K562AKAP8L
METTL1ENSG00000037897.12662.78560434.038300.610603.303001.94450K562APOBEC3C
METTL1ENSG00000037897.12242.08340321.97390-0.411402.014701.33670K562CPSF6
METTL1ENSG00000037897.12562.85630343.413600.712708.331407.28090K562DDX47
METTL1ENSG00000037897.12283.22740169.563800.740002.975301.92770K562EIF3A
METTL1ENSG00000037897.12292.75820141.187201.052008.891907.46720K562EIF3G
METTL1ENSG00000037897.12228.1721098.926601.205608.316206.89420K562EIF4A3
METTL1ENSG00000037897.12332.84250102.877201.6938020.1465018.62770K562FXR1
METTL1ENSG00000037897.12704.74160558.820400.334601.993101.34580K562GRSF1
METTL1ENSG00000037897.1271.06740217.25890-1.612204.162702.57520K562HNRNPA2B1
METTL1ENSG00000037897.12187.70330117.649200.673902.602801.40840K562HNRNPK
METTL1ENSG00000037897.12476.45220310.316300.618504.919803.46530K562HNRNPL
METTL1ENSG00000037897.12102.08360191.37140-0.906604.304803.24510K562KIF1C
METTL1ENSG00000037897.12263.01990123.812601.086905.945204.69650K562MAGOH
METTL1ENSG00000037897.12111.54550183.17530-0.715602.736201.66780K562MAK16
METTL1ENSG00000037897.12285.64970123.554001.2090010.980709.03210K562MATR3
METTL1ENSG00000037897.1264.62760150.26460-1.217304.751603.49680K562NAA15
METTL1ENSG00000037897.1277.34730190.16800-1.297903.783702.57510K562PABPN1
METTL1ENSG00000037897.12225.72820379.79530-0.750604.772703.68320K562PCBP3
METTL1ENSG00000037897.12204.79150291.30050-0.508402.559601.79180K562POLR2G
METTL1ENSG00000037897.12297.17300120.295301.3046013.0762011.69020K562PTBP1
METTL1ENSG00000037897.12712.16790536.683000.408003.001402.15070K562PUF60
METTL1ENSG00000037897.1259.91220178.85390-1.577909.377008.42650K562PUS1
METTL1ENSG00000037897.1285.40890174.23630-1.028605.460704.65250K562RBM15
METTL1ENSG00000037897.12189.17730115.322300.714003.389202.45360K562RBM22
METTL1ENSG00000037897.12212.76790121.364500.809804.320002.91910K562NELFE
METTL1ENSG00000037897.12457.04050259.604400.815903.710802.45080K562SAFB
METTL1ENSG00000037897.1273.63990199.69900-1.439304.061602.47320K562SNRNP200
METTL1ENSG00000037897.12174.99190117.935000.569202.135201.50460K562SRSF1
METTL1ENSG00000037897.12261.83750349.61700-0.417102.641101.50360K562SRSF7
METTL1ENSG00000037897.12106.82590245.12820-1.198303.431801.31880K562SUGP2
METTL1ENSG00000037897.1288.23110238.94980-1.437303.490601.82610K562SUPT6H
METTL1ENSG00000037897.12560.08800292.203400.9386011.380209.72570K562SYNCRIP
METTL1ENSG00000037897.12173.79480270.15430-0.636403.141902.05330K562UCHL5
METTL1ENSG00000037897.12179.58480267.74780-0.576202.128501.36220K562WDR3
METTL1ENSG00000037897.12365.68640278.414200.393302.470601.66840K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for METTL1 is not available on ENCORE database.





14 RBPs can affect the splicing of METTL1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.309000.000090.00290excludedK562SF3B4
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.164000.000000.00431includedK562SMNDC1
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.100000.000050.00707excludedHepG2TBRG4
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.214000.000250.01278excludedK562FXR1
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.069000.000430.04420includedHepG2SRSF7
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.066000.001200.04897excludedHepG2MATR3
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.267000.000010.00190includedHepG2DDX6
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.086000.000650.04724includedK562HNRNPL
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.127000.000070.07440includedK562ZRANB2
SEMETTL1ENSG00000037897.12chr12-5816526258165388581648765816504058165756581658990.278000.000000.00004includedK562HLTF
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.101000.000000.00162excludedHepG2DDX55
SEMETTL1ENSG00000037897.12chr12-581652625816538858164851581650405816575658165899-0.095000.000080.03834excludedHepG2XRN2
SEMETTL1ENSG00000037897.12chr12-581652625816538858164876581650405816575658165899-0.145000.003390.09661excludedHepG2HNRNPC
A3SSMETTL1ENSG00000037897.12chr12-5816308658163218581630865816318758163347581634610.273000.000000.00002longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for METTL1 is not avaliable on ENCORE database.