DCTN2

General Information

Gene Name       : DCTN2
Gene ID             : ENSG00000175203.15
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: DCTN2)


Antibody Validation

View validation images for all DCTN2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878DCTN2ENSG00000175203.1587.44116.65102.05ENCSR000AEF
HepG2DCTN2ENSG00000175203.1556.7758.0857.42ENCSR985KAT
K562DCTN2ENSG00000175203.15184.37181.56182.97ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq DCTN2-BGHcLV29-HepG2ENCSR146ITQDCTN2HepG2ReleasedENCSR462MFN   CRISPRKD% = 66.6,64.6






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
DCTN2ENCAB127PWUA303-488A1Released, ENCODE4BGHcLV29-65, Released, ENCODE4BGKcLV35-23, Released, ENCODE4
DCTN2ENCAB872CODA303-489A1Released, ENCODE4BGHcLV29-65, Released, ENCODE4BGKcLV35-23, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
DCTN2K562CRISPRBGKcLV28-65,BGKcLV28-66BGKcLV28-1,BGKcLV28-239.1,36.7NotSatisfiedENCODE4
DCTN2K562CRISPRDCTN2-BGKcLV35-23,DCTN2-BGKcLV35-24NT-BGKcLV35-1,NT-BGKcLV35-255.2,57.3SubmittingENCORE2
DCTN2HepG2CRISPRENCSR146ITQENCSR462MFNDCTN2-BGHcLV29-65,DCTN2-BGHcLV29-66NT-BGHcLV29-1,NT-BGHcLV29-266.6,64.6ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DCTN2Product_ID: A303-488A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DCTN2-human
DCTN2-HEPG2-CRISPR-A303-488A.png<br>Caption: Western blot following CRISPR against DCTN2 in HepG2 whole cell lysate using DCTN2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DCTN2. DCTN2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
Bethyl_A303-488A_1_DCTN2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DCTN2 specific antibody, A303-488A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DCTN2-specific antibody, A303-488A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
DCTN2-K562-CRISPR-A303-488A.png<br>Caption: Western blot following CRISPR against DCTN2 in K562 whole cell lysate using DCTN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DCTN2. DCTN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
DCTN2Product_ID: A303-489A
Lot_ID: 1
Source: Bethyl Labs
Target Name: DCTN2-human
Bethyl_A303-489A_1_DCTN2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DCTN2 specific antibody, A303-489A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the DCTN2-specific antibody, A303-489A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
DCTN2-K562-CRISPR-A303-489A.png<br>Caption: Western blot following CRISPR against DCTN2 in K562 whole cell lysate using DCTN2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against DCTN2. DCTN2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for DCTN2 is not avaliable on ENCORE database.



Proteins bound to RNA

  • DCTN2 RNA is bound by 26 proteins.  







eCLIP assay for DCTN2 is not avaliable on ENCORE database.





26 proteins are bound to DCTN2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr125792821857928290-3'-UTR,CDS,intron-Distal,intron-Proximal4.861685.40008K562SF3B4
chr125792637157926401-3'-UTR,CDS,intron-Distal,intron-Proximal5.410749.12048K562YBX3
chr125792696257927025-3'-UTR,CDS,intron-Distal,intron-Proximal5.527318.88118K562AQR
chr125792817057928239-3'-UTR,CDS,intron-Distal,intron-Proximal3.969375.80295HepG2PPIG
chr125792675657926858-3'-UTR,CDS,intron-Distal,intron-Proximal3.359977.18622K562PRPF8
chr125792818057928254-3'-UTR,CDS,intron-Distal,intron-Proximal4.616374.45977HepG2U2AF2
chr125792410857924154-3'-UTR,intron-Distal,intron-Proximal5.3158024.75473K562PABPC4
chr125792884257928938-3'-UTR,CDS,intron-Distal,intron-Proximal3.116305.02671K562PUM1
chr125792640157926410-3'-UTR,CDS,intron-Distal4.804785.60746K562YBX3
chr125792600657926060-3'-UTR,CDS,intron-Distal,intron-Proximal4.946774.48364K562DDX24
chr125792676357926818-3'-UTR,CDS,intron-Distal,intron-Proximal3.726983.06676K562U2AF2
chr125792774157927753-3'-UTR,CDS,intron-Distal,intron-Proximal4.342144.30417K562YBX3
chr125792819957928230-3'-UTR,CDS,intron-Distal,intron-Proximal3.963303.32022K562UCHL5
chr125792805957928102-3'-UTR,CDS,intron-Distal,intron-Proximal4.572514.35676HepG2PRPF8
chr125792635557926371-3'-UTR,CDS,intron-Distal,intron-Proximal4.457294.30417K562YBX3
chr125792808657928140-3'-UTR,CDS,intron-Distal,intron-Proximal3.031993.38325HepG2IGF2BP1
chr125792420457924319-3'-UTR,intron-Distal,intron-Proximal3.941605.91795HepG2UPF1
chr125792423857924305-3'-UTR,intron-Distal,intron-Proximal4.9076910.95701HepG2SUB1
chr125792773657927788-3'-UTR,CDS,intron-Distal,intron-Proximal3.124033.41653K562SND1
chr125792422657924293-3'-UTR,intron-Distal4.1262718.38865K562PABPC4
chr125792607257926086-3'-UTR,CDS,intron-Distal3.0177210.00730K562YBX3
chr125792579757925840-3'-UTR,CDS,intron-Distal,intron-Proximal3.4344810.34414K562YBX3
chr125792673757926815-3'-UTR,CDS,intron-Distal,intron-Proximal4.410295.93840HepG2PRPF8
chr125792606257926113-3'-UTR,CDS,intron-Distal,intron-Proximal4.174113.77802HepG2U2AF2
chr125792408157924108-3'-UTR,intron-Distal,intron-Proximal,polyA_site6.007267.49093K562PABPC4
chr125792651457926550-3'-UTR,CDS,intron-Distal,intron-Proximal3.515934.41267HepG2PPIG
chr125792635557926428-3'-UTR,CDS,intron-Distal,intron-Proximal3.086874.57537K562LIN28B
chr125792783057927881-3'-UTR,CDS,intron-Distal,intron-Proximal3.756355.11568K562PUM1
chr125792415457924189-3'-UTR,intron-Distal3.837887.65013K562PABPC4
chr125792679257926873-3'-UTR,CDS,intron-Distal,intron-Proximal3.953536.67591K562SF3B4
chr125792635557926427-3'-UTR,CDS,intron-Distal,intron-Proximal3.832063.86355HepG2PPIG
chr125792886457928945-3'-UTR,CDS,intron-Distal,intron-Proximal3.360846.47255K562BUD13
chr125792807057928118-3'-UTR,CDS,intron-Distal,intron-Proximal4.823824.74403K562PUM1
chr125792421757924292-3'-UTR,intron-Distal3.532946.35665HepG2LARP4
chr125792775457927797-3'-UTR,CDS,intron-Distal,intron-Proximal3.120094.53299K562YBX3
chr125792413157924216-3'-UTR,intron-Distal,intron-Proximal3.093445.40041HepG2LARP4
chr125792879657928844-3'-UTR,CDS,intron-Distal,intron-Proximal5.720949.06713HepG2PRPF8
chr125792807457928144-3'-UTR,CDS,intron-Distal,intron-Proximal3.356613.97184HepG2G3BP1
chr125792434157924483-3'-UTR,CDS,intron-Distal,intron-Proximal5.901087.10959K562PCBP1
chr125792656957926643-3'-UTR,CDS,intron-Distal,intron-Proximal3.693515.32169K562EFTUD2
chr125793982057939854-CDS,intron-Distal3.198077.59396K562YBX3
chr125793230257932337-CDS,intron-Distal,intron-Proximal3.817866.98930HepG2U2AF2
chr125793515557935232-intron-Distal,intron-Proximal3.381124.39249K562RBFOX2
chr125792697457927019-intron-Distal,intron-Proximal4.656594.58968K562PRPF8
chr125793499057935047-intron-Distal4.586554.70434HepG2RBFOX2
chr125794020057940250-intron-Distal4.780314.33458HepG2XPO5
chr125793499457935058-intron-Distal,intron-Proximal5.028857.25108K562RBFOX2
chr125793455057934599-intron-Distal4.160373.27618K562RBFOX2
chr125793230857932387-intron-Distal,intron-Proximal4.153957.49381HepG2PCBP2
chr125793505857935101-intron-Distal,intron-Proximal3.932537.35293K562RBFOX2
chr125792628957926354-intron-Distal,intron-Proximal5.139916.45599K562PRPF8
chr125793254957932711-intron-Distal3.368207.15356HepG2QKI
chr125792644057926475-intron-Distal,intron-Proximal5.082314.83371K562EFTUD2
chr125793233757932390-intron-Distal,intron-Proximal6.4288615.59508HepG2U2AF2
chr125793233657932388-intron-Distal,intron-Proximal4.300243.67656K562U2AF2
chr125794056957940608-intron-Distal3.912383.15006K562RBM15
chr125793234157932388-intron-Distal,intron-Proximal3.837133.47425HepG2U2AF1
chr125793504757935115-intron-Distal,intron-Proximal4.217076.03807HepG2RBFOX2
chr125792879257928821-intron-Distal,intron-Proximal5.618608.03507K562AQR
chr125792882157928838-intron-Distal,intron-Proximal5.123635.65342K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for DCTN2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for DCTN2 is not available on ENCORE database.

  • 66 RBPs can affect the levels of DCTN2.  








The Knockdown/knockout RNA-Seq assay for DCTN2 is not available on ENCORE database.





66 RBPs can affect the gene expression of DCTN2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
DCTN2ENSG00000175203.111971.874701456.721400.436708.580707.63070HepG2ACO1
DCTN2ENSG00000175203.112870.929702252.615500.349802.299501.80990HepG2AKAP1
DCTN2ENSG00000175203.111826.789601463.549800.319704.269903.33160HepG2AKAP8L
DCTN2ENSG00000175203.112337.079401863.829700.326302.348501.62220HepG2ASCC1
DCTN2ENSG00000175203.112576.674302245.289700.198502.041401.43500HepG2AUH
DCTN2ENSG00000175203.111309.595701758.25170-0.425004.090003.37840HepG2BOP1
DCTN2ENSG00000175203.112347.737801770.773100.406803.546602.67860HepG2CIRBP
DCTN2ENSG00000175203.11833.713801952.20170-1.2275013.3060011.68300HepG2CNOT7
DCTN2ENSG00000175203.112586.648601922.973200.427607.833706.85560HepG2CELF1
DCTN2ENSG00000175203.11940.961801089.15540-0.211002.001601.43040HepG2DNAJC2
DCTN2ENSG00000175203.111301.438301017.631700.354802.416101.55210HepG2EEF2
DCTN2ENSG00000175203.111396.975301917.34270-0.456802.798302.10580HepG2EIF4B
DCTN2ENSG00000175203.111736.307402039.85360-0.232402.373401.58850HepG2EIF4G1
DCTN2ENSG00000175203.112531.913301903.729000.411302.939402.14580HepG2FIP1L1
DCTN2ENSG00000175203.11806.015901616.93750-1.004405.224003.83350HepG2FTO
DCTN2ENSG00000175203.111003.02850735.523700.447403.478102.46050HepG2GEMIN5
DCTN2ENSG00000175203.112410.319501829.788800.397403.059802.39110HepG2GPKOW
DCTN2ENSG00000175203.111261.649901807.29030-0.518503.256302.04170HepG2GRSF1
DCTN2ENSG00000175203.111443.669601866.49430-0.370603.107302.36820HepG2HNRNPK
DCTN2ENSG00000175203.112288.705901746.201200.390203.327902.64480HepG2HNRNPLL
DCTN2ENSG00000175203.111600.531001252.235900.353906.239705.18230HepG2ILF2
DCTN2ENSG00000175203.111738.153802050.08470-0.238102.026801.37150HepG2NOL12
DCTN2ENSG00000175203.111058.790101933.32200-0.8687026.0103024.47150HepG2PCBP1
DCTN2ENSG00000175203.111321.128401526.50820-0.208501.988801.32510HepG2PRPF4
DCTN2ENSG00000175203.111658.684101967.22540-0.246102.778302.22990HepG2PTBP1
DCTN2ENSG00000175203.112414.628702076.002400.217902.056201.47250HepG2PUM2
DCTN2ENSG00000175203.112546.762902154.721600.241102.592002.08620HepG2RBM22
DCTN2ENSG00000175203.111634.202901933.61670-0.242701.914901.34470HepG2RBM25
DCTN2ENSG00000175203.111768.157902039.06470-0.205702.170501.61560HepG2RECQL
DCTN2ENSG00000175203.111113.01320803.825300.469404.604903.83360HepG2RPS19
DCTN2ENSG00000175203.111429.132401960.92200-0.456402.906502.08730HepG2RPS3A
DCTN2ENSG00000175203.11972.706801344.68520-0.467204.394003.02500HepG2RPS5
DCTN2ENSG00000175203.111356.788501846.53240-0.444602.998301.63750HepG2SART3
DCTN2ENSG00000175203.112227.194401707.042100.383602.297101.68210HepG2SF3A3
DCTN2ENSG00000175203.111626.082701876.65320-0.206802.236801.59500HepG2SLBP
DCTN2ENSG00000175203.111047.350001273.20040-0.281703.503802.79380HepG2SMN1
DCTN2ENSG00000175203.113173.356502433.631700.382807.903507.17680HepG2SRFBP1
DCTN2ENSG00000175203.111499.093301727.33060-0.204502.342101.69770HepG2SRSF7
DCTN2ENSG00000175203.112493.753601159.101301.1052016.7292015.31190HepG2SUPV3L1
DCTN2ENSG00000175203.111647.287801921.54500-0.222202.021401.52910HepG2TAF15
DCTN2ENSG00000175203.111724.013101191.334700.533107.811907.04940HepG2TIAL1
DCTN2ENSG00000175203.111451.609601061.187700.451902.285001.37900HepG2UPF2
DCTN2ENSG00000175203.111700.912101461.598300.218702.305101.74720HepG2UTP3
DCTN2ENSG00000175203.112089.465401506.479800.4719010.693709.51630HepG2ZC3H8
DCTN2ENSG00000175203.112728.877001664.984900.7127028.2590026.97740HepG2ZNF622
DCTN2ENSG00000175203.113039.854905013.62440-0.7219012.6550011.53190K562AQR
DCTN2ENSG00000175203.112154.022802767.53000-0.361603.134902.20480K562BOP1
DCTN2ENSG00000175203.113130.498104289.37040-0.454402.356101.38940K562CNOT7
DCTN2ENSG00000175203.111813.418202248.26490-0.310102.452801.59460K562EFTUD2
DCTN2ENSG00000175203.111691.376902406.96320-0.509004.229402.91280K562EIF3D
DCTN2ENSG00000175203.112201.752502668.71320-0.277502.284101.53570K562EIF4A3
DCTN2ENSG00000175203.114888.013505805.48820-0.248203.890802.77930K562EIF4G2
DCTN2ENSG00000175203.112791.374102329.499500.260902.791601.49630K562EWSR1
DCTN2ENSG00000175203.113813.751303070.155700.312802.862601.99810K562FIP1L1
DCTN2ENSG00000175203.111555.616602537.67990-0.706009.164507.40560K562FTO
DCTN2ENSG00000175203.112394.969302775.34530-0.212702.285001.72650K562FXR1
DCTN2ENSG00000175203.111523.556902096.53340-0.460603.133202.37230K562G3BP2
DCTN2ENSG00000175203.112228.796202809.63630-0.334103.213802.36050K562GPKOW
DCTN2ENSG00000175203.113162.091304414.64250-0.481407.085205.86660K562GRSF1
DCTN2ENSG00000175203.113254.872103872.59320-0.250702.368801.37920K562HNRNPA1
DCTN2ENSG00000175203.112025.414202650.09860-0.387804.433503.69610K562HNRNPC
DCTN2ENSG00000175203.115994.435404162.374600.526107.201705.93260K562HSPD1
DCTN2ENSG00000175203.111707.585202398.95650-0.490403.086302.09330K562KRR1
DCTN2ENSG00000175203.112189.914602742.49880-0.324603.105602.17510K562METAP2
DCTN2ENSG00000175203.111974.277003573.13880-0.8559035.3389033.04400K562MTPAP
DCTN2ENSG00000175203.112122.935402853.09550-0.426504.861103.55520K562PCBP2
DCTN2ENSG00000175203.111786.457302187.82690-0.292402.174001.50510K562PRPF8
DCTN2ENSG00000175203.115342.650206334.06720-0.245602.190401.46570K562PUF60
DCTN2ENSG00000175203.115190.888706738.39880-0.376406.422404.74110K562PUM1
DCTN2ENSG00000175203.115265.595206601.14890-0.326106.211304.75520K562PUM2
DCTN2ENSG00000175203.117469.596606163.005400.277302.655302.11980K562PUS1
DCTN2ENSG00000175203.112482.141402987.00990-0.267102.115301.38510K562RBM22
DCTN2ENSG00000175203.112011.333702671.89360-0.409703.209202.15960K562SF3B1
DCTN2ENSG00000175203.111510.949802098.21590-0.473706.775505.52560K562U2AF2
DCTN2ENSG00000175203.113740.222304727.36520-0.337902.530401.38730K562UTP3
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for DCTN2 is not available on ENCORE database.





10 RBPs can affect the splicing of DCTN2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEDCTN2ENSG00000175203.11chr12-579398105793987957929531579296285794081057941114-0.053000.002530.04377excludedK562SF3B4
SEDCTN2ENSG00000175203.11chr12-579259955792609857925797579258895792635457926426-0.093000.000000.00002excludedHepG2U2AF1
SEDCTN2ENSG00000175203.11chr12-579398105793987957929531579296285794081057941114-0.125000.000000.00003excludedHepG2HNRNPK
SEDCTN2ENSG00000175203.11chr12-5793203557932247579295315792962857939810579398790.198000.000000.00000includedHepG2U2AF2
SEDCTN2ENSG00000175203.11chr12-579259955792609857925797579258895792635457926426-0.108000.000020.00055excludedHepG2U2AF2
SEDCTN2ENSG00000175203.11chr12-5793971557939911579295315792962857940810579409240.293000.000000.00002includedK562AQR
SEDCTN2ENSG00000175203.11chr12-579397155793987957929531579296285794081057940992-0.052000.006030.05428excludedK562AQR
SEDCTN2ENSG00000175203.11chr12-579259955792609857925797579258895792635457926426-0.066000.000000.00000excludedK562AQR
SEDCTN2ENSG00000175203.11chr12-579259955792609857925797579258895792635457926426-0.053000.000450.03337excludedHepG2FTO
SEDCTN2ENSG00000175203.11chr12-5793226557932307579295315792962857939810579398790.074000.000000.00000includedK562U2AF1
SEDCTN2ENSG00000175203.11chr12-5793971557939879579295315792962857940810579409030.089000.000010.00362includedHepG2LARP4
SEDCTN2ENSG00000175203.11chr12-579398105793987957929531579296285794081057941114-0.087000.000060.00180excludedK562U2AF2
SEDCTN2ENSG00000175203.11chr12-579398105793987957929531579296285794081057941114-0.092000.000000.00025excludedK562HNRNPK
SEDCTN2ENSG00000175203.11chr12-579259955792609857925797579258895792635457926426-0.115000.000000.00000excludedK562HNRNPC
A3SSDCTN2ENSG00000175203.11chr12-5792676657926913579267665792680557927020579270860.051000.004380.08637longerHepG2U2AF2
A3SSDCTN2ENSG00000175203.11chr12-5792779157927974579277915792788057928074579282350.058000.000000.00000longerK562AQR
A3SSDCTN2ENSG00000175203.11chr12-5792599557926131579259955792609857926354579264260.069000.000000.00000longerK562AQR
A3SSDCTN2ENSG00000175203.11chr12-5793981057939958579398105793987957940810579409240.086000.000000.00000longerK562AQR
RIDCTN2ENSG00000175203.11chr12-5792676657927086579267665792680557927020579270860.100000.000000.00006includedHepG2U2AF2
RIDCTN2ENSG00000175203.11chr12-5792676657927086579267665792680557927020579270860.264000.000330.01225includedHepG2FTO
RIDCTN2ENSG00000175203.11chr12-5792676657927086579267665792680557927020579270860.067000.000000.00001includedHepG2NCBP2
RIDCTN2ENSG00000175203.11chr12-5792676657927086579267665792680557927020579270860.057000.000000.00000includedK562AQR
MXEDCTN2ENSG00000175203.11chr12-57927020579270865792807457928235579267665792680557928838579289370.122000.000590.015991includedK562SUPV3L1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for DCTN2 is not avaliable on ENCORE database.