CNOT10

General Information

Gene Name       : CNOT10
Gene ID             : ENSG00000182973.18
Essential Gene : No

Functions

Localization

Domains (AlphaFold: CNOT10)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878CNOT10ENSG00000182973.1836.4930.1133.30ENCSR000AEF
HepG2CNOT10ENSG00000182973.1816.4322.8019.62ENCSR985KAT
K562CNOT10ENSG00000182973.1862.8874.4668.67ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq CNOT10-BGKcLV21-K562ENCSR644LYCCNOT10K562ReleasedENCSR854OFV   CRISPRKD% = 74.8,76.6
knockdown followed by RNA-seq CNOT10-BGHcLV22-HepG2ENCSR002NQQCNOT10HepG2ReleasedENCSR704VPL   CRISPRKD% = 92.2,89.5






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
CNOT10ENCAB805LWEA304-899A1BGHcLV22-9, Released, ENCODE4BGKcLV21-7, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
CNOT10K562CRISPRENCSR644LYCENCSR854OFVCNOT10-BGKcLV21-7,CNOT10-BGKcLV21-8NT-BGKcLV21-1,NT-BGKcLV21-274.8,76.6ReleasedENCODE4
CNOT10HepG2CRISPRENCSR002NQQENCSR704VPLCNOT10-BGHcLV22-9,CNOT10-BGHcLV22-10NT-BGHcLV22-1,NT-BGHcLV22-292.2,89.5ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
CNOT10Product_ID: A304-899A
Lot_ID: 1
Source: Bethyl Labs
Target Name: CNOT10-human		 CNOT10-HEPG2-CRISPR-A304-899A.png<br>Caption: Western blot following CRISPR against CNOT10 in HepG2 whole cell lysate using CNOT10 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CNOT10. CNOT10 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 CNOT10-K562-CRISPR-A304-899A.png<br>Caption: Western blot following CRISPR against CNOT10 in K562 whole cell lysate using CNOT10 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against CNOT10. CNOT10 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for CNOT10 is not avaliable on ENCORE database.



Proteins bound to RNA
  • CNOT10 RNA is bound by 18 proteins.  







eCLIP assay for CNOT10 is not avaliable on ENCORE database.





18 proteins are bound to CNOT10 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr33281131132811423+3'-UTR,CDS,intron-Distal,intron-Proximal3.560865.75231K562AQR
chr33281500132815030+3'-UTR,CDS3.7579212.74366K562YBX3
chr33276918732769234+3'-UTR,5'-UTR,CDS3.761624.09885HepG2BCLAF1
chr33280428032804374+3'-UTR,CDS,intron-Proximal3.772283.16390K562SRSF1
chr33280629132806340+3'-UTR,CDS,intron-Proximal4.780035.93128HepG2PRPF8
chr33281498132815001+3'-UTR,CDS3.120629.91876K562YBX3
chr33280622932806278+3'-UTR,CDS3.670155.78947K562YBX3
chr33280423532804303+3'-UTR,CDS3.698833.14999HepG2GRWD1
chr33281514332815170+3'-UTR5.3494712.66140K562YBX3
chr33280092032800998+3'-UTR,CDS,intron-Proximal4.622364.20763HepG2U2AF2
chr33281504232815159+3'-UTR,CDS4.9068610.95110K562PABPC4
chr33281512232815143+3'-UTR3.7818910.74560K562YBX3
chr33277492632775014+5'-UTR,CDS4.3059313.46848K562PUM2
chr33275775032757800+5'-UTR,CDS3.026883.71797HepG2BCLAF1
chr33277641932776478+5'-UTR,CDS,intron-Proximal4.641265.35117HepG2PRPF8
chr33272685332726941+5'-UTR4.788737.13477K562DDX3X
chr33272667832726770+5'-UTR3.048693.36383K562NCBP2
chr33275024432750294+5'-UTR,CDS3.650844.51739HepG2BCLAF1
chr33272669732726853+5'-UTR3.352675.96481K562DDX3X
chr33274630232746362+5'-UTR,CDS,intron-Distal,intron-Proximal3.570733.69978K562DDX24
chr33275016132750218+5'-UTR,CDS,intron-Proximal3.605955.95375K562ZNF622
chr33275476932754862+CDS,intron-Distal,intron-Proximal4.311923.98311K562IGF2BP1
chr33275471832754823+CDS,intron-Distal,intron-Proximal4.8223030.33866K562PUM2
chr33280069132800791+intron-Distal3.749747.64049K562KHSRP
chr33280637232806429+intron-Distal,intron-Proximal3.384433.47285K562PRPF8
chr33276256532762611+intron-Distal3.882327.33269K562PTBP1
chr33279917732799297+intron-Distal3.294803.35585HepG2QKI
chr33276175232761796+intron-Distal,intron-Proximal4.017013.77594K562RBM22
chr33280630132806343+intron-Proximal4.411085.47926K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for CNOT10 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for CNOT10 is not available on ENCORE database.

  • 61 RBPs can affect the levels of CNOT10.  








The Knockdown/knockout RNA-Seq assay for CNOT10 is not available on ENCORE database.





61 RBPs can affect the gene expression of CNOT10 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
CNOT10ENSG00000182973.14502.07820732.08740-0.544103.323202.74780HepG2AKAP1
CNOT10ENSG00000182973.14608.26490755.02390-0.311802.531001.86960HepG2AUH
CNOT10ENSG00000182973.14515.37150675.62190-0.390602.493101.92170HepG2BOP1
CNOT10ENSG00000182973.14577.23570745.51920-0.369103.893403.03820HepG2DAZAP1
CNOT10ENSG00000182973.14552.47250408.368100.435902.863901.93180HepG2DDX24
CNOT10ENSG00000182973.14333.73270497.94840-0.577303.264802.43470HepG2DDX55
CNOT10ENSG00000182973.14330.38000451.16540-0.449503.219202.46570HepG2DNAJC21
CNOT10ENSG00000182973.14325.61890447.16000-0.457603.912303.16420HepG2DNAJC2
CNOT10ENSG00000182973.14373.54830579.14220-0.632604.397503.65940HepG2EIF2S1
CNOT10ENSG00000182973.14390.90560640.04960-0.711405.294704.41010HepG2EIF4A3
CNOT10ENSG00000182973.14527.65900704.53210-0.417102.843702.01840HepG2EIF4G2
CNOT10ENSG00000182973.141188.85370866.873300.455606.392505.54080HepG2ETF1
CNOT10ENSG00000182973.14566.02100438.315200.368802.330301.56460HepG2EWSR1
CNOT10ENSG00000182973.14380.84400599.02290-0.653404.697703.84080HepG2G3BP1
CNOT10ENSG00000182973.14386.68640506.63840-0.389802.095901.48240HepG2HNRNPC
CNOT10ENSG00000182973.14357.37380606.45580-0.763006.626405.57910HepG2HNRNPK
CNOT10ENSG00000182973.14482.94180679.12470-0.491805.848904.89950HepG2HNRNPL
CNOT10ENSG00000182973.14436.95990608.91030-0.478702.947802.25460HepG2IGF2BP2
CNOT10ENSG00000182973.14683.41480900.67130-0.398202.073901.36270HepG2LARP4
CNOT10ENSG00000182973.14584.81830739.25290-0.338102.646301.90060HepG2NOL12
CNOT10ENSG00000182973.14583.11030748.54610-0.360302.760902.09080HepG2PUM2
CNOT10ENSG00000182973.14657.55270456.881800.525205.997005.15270HepG2PUS1
CNOT10ENSG00000182973.14505.08230693.57770-0.457503.489502.53590HepG2RBM15
CNOT10ENSG00000182973.14257.23020379.94850-0.562702.766501.98010HepG2RRP9
CNOT10ENSG00000182973.14324.38730448.20560-0.466403.706902.98060HepG2SAFB2
CNOT10ENSG00000182973.14515.71020664.13910-0.364902.148401.52170HepG2SF3B1
CNOT10ENSG00000182973.14510.27360676.59640-0.407004.094903.26490HepG2SLBP
CNOT10ENSG00000182973.14568.01740696.72780-0.294702.327801.74780HepG2SRSF5
CNOT10ENSG00000182973.14599.20860710.51390-0.245801.773301.34040HepG2SSB
CNOT10ENSG00000182973.14373.02690545.63570-0.548703.469602.53770HepG2TIA1
CNOT10ENSG00000182973.14374.30220489.06640-0.385802.415901.94330HepG2TIAL1
CNOT10ENSG00000182973.14352.66820493.40960-0.484504.201203.53450HepG2TRIM56
CNOT10ENSG00000182973.14550.23250689.38470-0.325302.115001.35940HepG2TUFM
CNOT10ENSG00000182973.14686.71780521.427900.397102.562701.95590HepG2U2AF1
CNOT10ENSG00000182973.14210.68920292.81420-0.474902.184301.50890HepG2UCHL5
CNOT10ENSG00000182973.14728.96810531.095800.456804.646303.90190HepG2UTP3
CNOT10ENSG00000182973.14505.10660605.00000-0.260301.925401.44690HepG2ZNF622
CNOT10ENSG00000182973.142218.840402898.75120-0.385607.299806.44230K562AGGF1
CNOT10ENSG00000182973.142506.167103016.25640-0.267302.221201.63380K562AKAP8L
CNOT10ENSG00000182973.142651.052603164.73070-0.255502.991502.04360K562ATP5C1
CNOT10ENSG00000182973.141904.904202202.06700-0.209102.323001.44470K562BCLAF1
CNOT10ENSG00000182973.146896.227305715.953900.270703.332502.26140K562CELF1
CNOT10ENSG00000182973.141389.747201854.91500-0.416504.648503.24730K562DDX3X
CNOT10ENSG00000182973.144748.820303746.274700.342006.741205.78900K562DDX47
CNOT10ENSG00000182973.141540.563701880.62690-0.287803.142401.72450K562DDX6
CNOT10ENSG00000182973.141573.197901972.77650-0.326502.392801.54550K562EFTUD2
CNOT10ENSG00000182973.142287.705902838.50250-0.311204.237003.37560K562EIF2S2
CNOT10ENSG00000182973.141472.864102112.41600-0.520304.169402.86880K562EIF3D
CNOT10ENSG00000182973.142220.894301724.691100.364704.617703.60170K562EIF3G
CNOT10ENSG00000182973.141450.837901937.90990-0.417604.225203.21190K562EIF4A3
CNOT10ENSG00000182973.143200.923802611.466400.293502.970401.85550K562HNRNPA1
CNOT10ENSG00000182973.141802.998702488.67650-0.465004.451902.85470K562HNRNPK
CNOT10ENSG00000182973.142733.400903385.61580-0.308703.102801.97880K562HNRNPL
CNOT10ENSG00000182973.143398.449102891.971000.232702.560401.69950K562HNRNPU
CNOT10ENSG00000182973.142047.309502618.52020-0.355003.611102.64340K562MAGOH
CNOT10ENSG00000182973.141738.751202329.98130-0.422304.515603.25990K562PCBP2
CNOT10ENSG00000182973.145060.253003065.852200.7228010.036008.45730K562PCBP3
CNOT10ENSG00000182973.143832.571402351.567300.7046015.7028013.87440K562POLR2G
CNOT10ENSG00000182973.142208.821103896.92740-0.819102.723101.30750K562PPP1R8
CNOT10ENSG00000182973.142399.647102991.48190-0.318002.705502.16670K562PUS1
CNOT10ENSG00000182973.141552.086101918.93580-0.306102.698901.38440K562RBM25
CNOT10ENSG00000182973.141255.610601626.53700-0.373403.263702.12670K562SRFBP1
CNOT10ENSG00000182973.141784.063302493.87440-0.483206.605105.52170K562SRSF1
CNOT10ENSG00000182973.141901.029202434.78430-0.357004.018103.00490K562TIA1
CNOT10ENSG00000182973.141707.394902025.19610-0.246302.167201.45680K562U2AF2
CNOT10ENSG00000182973.142426.483701967.346900.302502.337301.41030K562UCHL5
CNOT10ENSG00000182973.142566.343403340.03650-0.380107.827906.69280K562UPF1
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for CNOT10 is not available on ENCORE database.





17 RBPs can affect the splicing of CNOT10 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SECNOT10ENSG00000182973.14chr3+328042243280446532800949328010633280594632805982-0.060000.002290.04063excludedK562SRSF1
SECNOT10ENSG00000182973.14chr3+328059463280598232804224328043553280617332806301-0.108000.000000.00000excludedK562SF3B4
SECNOT10ENSG00000182973.14chr3+328059463280598232804224328043553280617332806301-0.069000.002460.02993excludedHepG2U2AF2
SECNOT10ENSG00000182973.14chr3+327616053276172332758645327587293276694132767091-0.071000.000000.00000excludedK562AQR
SECNOT10ENSG00000182973.14chr3+3277890132778982327762913277646832800949328010630.127000.000030.00248includedK562FXR1
SECNOT10ENSG00000182973.14chr3+327789013277898232776291327764683280094932801063-0.121000.007430.08388excludedK562PUS1
SECNOT10ENSG00000182973.14chr3+3277890132778982327762913277646832800949328010630.145000.001600.05939includedHepG2MATR3
SECNOT10ENSG00000182973.14chr3+328059463280598232804224328043553280617332806301-0.076000.000020.00061excludedK562U2AF2
SECNOT10ENSG00000182973.14chr3+327789013277898232776291327764683280094932801063-0.138000.002700.04444excludedK562U2AF2
SECNOT10ENSG00000182973.14chr3+3272726532727420327268553272697432745359327454540.057000.001380.04823includedK562TARDBP
SECNOT10ENSG00000182973.14chr3+327762913277646832774914327750363280094932801063-0.121000.000090.00556excludedK562HNRNPU
SECNOT10ENSG00000182973.14chr3+327463023274646432745359327454543275016132750312-0.052000.000060.00408excludedK562HNRNPU
SECNOT10ENSG00000182973.14chr3+327789013277898232776291327764683280094932801063-0.123000.004570.08688excludedK562AGGF1
SECNOT10ENSG00000182973.14chr3+3277890132778982327762913277646832800949328010630.109000.000310.07110includedK562SERBP1
SECNOT10ENSG00000182973.14chr3+328059463280598232804224328043553280617332806301-0.060000.000440.00958excludedK562HNRNPC
A3SSCNOT10ENSG00000182973.14chr3+3274533432745454327453593274545432726855327269740.083000.000000.00000longerK562AQR
MXECNOT10ENSG00000182973.14chr3+32758645327587293276160532761723327577163275780332766941327670910.118000.000450.035771includedK562LARP4
MXECNOT10ENSG00000182973.14chr3+3277890132778982328009493280106332776291327764683280422432804355-0.094000.002850.067672includedK562RBM15
MXECNOT10ENSG00000182973.14chr3+3277890132778982328009493280106332776291327764683280594632805982-0.132000.002270.038282includedK562PUS1
MXECNOT10ENSG00000182973.14chr3+32745334327454543274630232746464327268553272697432750161327503120.084000.000000.000001includedK562AQR
MXECNOT10ENSG00000182973.14chr3+32758614327587293276154832761723327577163275780332766941327670910.103000.000000.000001includedK562AQR
MXECNOT10ENSG00000182973.14chr3+32758614327587293276160532761723327577163275780332766941327670910.103000.000000.000001includedK562AQR
MXECNOT10ENSG00000182973.14chr3+32776291327764683277890132778982327749143277503632800949328010630.170000.000070.001781includedK562U2AF2
MXECNOT10ENSG00000182973.14chr3+3275864532758729327616053276172332757716327578033276694132767091-0.207000.001020.037892includedHepG2SF3A3
MXECNOT10ENSG00000182973.14chr3+3280422432804355328059463280598232800949328010633280617332806301-0.102000.004040.075922includedK562TARDBP
MXECNOT10ENSG00000182973.14chr3+32758645327587293276160532761723327577163275780332766941327670910.181000.005750.091151includedHepG2DDX55
MXECNOT10ENSG00000182973.14chr3+3277890132778982328009493280106332776291327764683280422432804355-0.054000.017470.083862includedHepG2U2AF2
MXECNOT10ENSG00000182973.14chr3+32778901327789823280094932801063327762913277646832805946328059820.070000.004660.076661includedK562SUPV3L1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for CNOT10 is not avaliable on ENCORE database.