BYSL

General Information

Gene Name       : BYSL
Gene ID             : ENSG00000112578.9
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: BYSL)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878BYSLENSG00000112578.915.5821.3918.48ENCSR000AEF
HepG2BYSLENSG00000112578.957.6838.8048.24ENCSR985KAT
K562BYSLENSG00000112578.948.9049.2649.08ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq BYSL-BGKcLV26-K562ENCSR012OCKBYSLK562ReleasedENCSR900GQL   CRISPRKD% = 69.6,61.6008935






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
BYSLENCAB273OKQA304-568A1Released, ENCODE4BGKcLV26-43, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
BYSLK562CRISPRENCSR012OCKENCSR900GQLBYSL-BGKcLV26-43,BYSL-BGKcLV26-44NT-BGKcLV26-1,NT-BGKcLV26-269.6,61.6008935ReleasedENCODE4
BYSLHepG2CRISPRBGHcLV27-27,BGHcLV27-28BGHcLV27-1,BGHcLV27-212.3,26.5NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
BYSLProduct_ID: A304-568A
Lot_ID: 1
Source: Bethyl Labs
Target Name: BYSL-human		 Bethyl_A304-568A_1_BYSL.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the BYSL specific antibody, A304-568A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the BYSL-specific antibody, A304-568A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 BYSL-K562-CRISPR-A304-568A.png<br>Caption: Western blot following CRISPR against BYSL in K562 whole cell lysate using BYSL specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BYSL. BYSL protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for BYSL is not avaliable on ENCORE database.



Proteins bound to RNA
  • BYSL RNA is bound by 10 proteins.  







eCLIP assay for BYSL is not avaliable on ENCORE database.





10 proteins are bound to BYSL RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr64189927341899294+3'-UTR,CDS3.819243.46958K562YBX3
chr64189956441899672+3'-UTR,CDS,intron-Distal,intron-Proximal4.332278.28249HepG2AQR
chr64190006141900133+3'-UTR,CDS,intron-Proximal4.901037.09786HepG2AQR
chr64189907141899186+3'-UTR,CDS,intron-Proximal3.358514.80460HepG2AQR
chr64189944341899485+3'-UTR,CDS,intron-Proximal4.132733.63072HepG2AQR
chr64188924641889361+5'-UTR,CDS3.572954.32211K562DDX3X
chr64189910141899144+CDS,intron-Proximal5.172066.75320K562AQR
chr64188939341889480+CDS4.976066.23323K562UCHL5
chr64189526241895317+CDS,intron-Proximal4.7766411.25775HepG2PRPF8
chr64189511841895265+CDS3.395084.80070K562GRWD1
chr64189524341895275+CDS,intron-Proximal4.485905.10960K562ZNF622
chr64189526741895293+CDS,intron-Proximal4.932626.04760K562AQR
chr64189521341895286+CDS,intron-Proximal3.534623.60157HepG2GRWD1
chr64189835041898391+CDS,intron-Proximal3.706664.21358HepG2U2AF2
chr64189511141895176+CDS,intron-Proximal4.540585.50697K562UCHL5
chr64189800741898051+CDS,intron-Distal,intron-Proximal4.784594.13847K562AQR
chr64189687841896946+intron-Distal3.829113.58660HepG2ILF3
chr64189065541890746+intron-Distal4.645064.57427HepG2PCBP2
chr64189872341898774+intron-Distal4.353733.12843K562PRPF8
chr64189696241897042+intron-Distal4.187913.77875HepG2ILF3
chr64189782041897866+intron-Distal,intron-Proximal4.245663.49003K562PRPF8
chr64189529341895331+intron-Proximal5.138506.75320K562AQR
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for BYSL is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for BYSL is not available on ENCORE database.

  • 74 RBPs can affect the levels of BYSL.  








The Knockdown/knockout RNA-Seq assay for BYSL is not available on ENCORE database.





74 RBPs can affect the gene expression of BYSL :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
BYSLENSG00000112578.5703.55840587.372700.260302.005901.47290HepG2ACO1
BYSLENSG00000112578.51399.38530839.197000.737606.949306.14850HepG2AKAP1
BYSLENSG00000112578.5529.28820694.34070-0.391602.242501.53160HepG2ASCC1
BYSLENSG00000112578.5620.17350407.712000.605004.967604.19670HepG2BOP1
BYSLENSG00000112578.5737.88260587.234500.329403.121402.42460HepG2CKAP4
BYSLENSG00000112578.5270.03120374.30750-0.471102.290901.65740HepG2CPSF6
BYSLENSG00000112578.51176.26700681.862300.7866011.2800010.15560HepG2CELF1
BYSLENSG00000112578.5309.07280543.23070-0.8136011.8737010.39110HepG2DDX19B
BYSLENSG00000112578.5411.65850561.95380-0.449003.759102.99290HepG2DKC1
BYSLENSG00000112578.5410.55640663.80100-0.693205.487104.66840HepG2EIF2S1
BYSLENSG00000112578.5496.96670377.066400.398202.503501.39550HepG2EIF2S2
BYSLENSG00000112578.5338.93750733.51900-1.1138012.3089010.99150HepG2EIF4A3
BYSLENSG00000112578.51122.24440714.296500.651704.520103.65150HepG2EIF4B
BYSLENSG00000112578.5482.99560692.92030-0.520704.173703.18440HepG2EIF4G2
BYSLENSG00000112578.5290.72430448.15780-0.624404.194103.35330HepG2HNRNPC
BYSLENSG00000112578.5464.58380634.00160-0.448504.002003.15520HepG2HNRNPF
BYSLENSG00000112578.5193.04930404.93730-1.068708.770107.71790HepG2HNRNPLL
BYSLENSG00000112578.5451.70940624.31440-0.466903.487402.68160HepG2IGF2BP1
BYSLENSG00000112578.51212.74440744.643600.703606.427205.49850HepG2MATR3
BYSLENSG00000112578.5425.75420275.588100.627402.508801.50360HepG2METAP2
BYSLENSG00000112578.5261.39600412.94920-0.659702.851301.86750HepG2MTPAP
BYSLENSG00000112578.5887.94400727.109300.288201.985001.33600HepG2NOL12
BYSLENSG00000112578.5484.52260694.05990-0.518504.237503.51790HepG2PUF60
BYSLENSG00000112578.5940.05450736.740500.351503.862003.18670HepG2PUM1
BYSLENSG00000112578.5657.22300825.96750-0.329702.501101.90190HepG2QKI
BYSLENSG00000112578.5640.826401004.26640-0.648109.098508.28310HepG2RBM22
BYSLENSG00000112578.5542.82330749.62460-0.465703.827703.04390HepG2RBM25
BYSLENSG00000112578.5784.76200574.699500.449302.816801.81640HepG2RBM27
BYSLENSG00000112578.5682.30200848.76500-0.315002.633602.10330HepG2NELFE
BYSLENSG00000112578.5620.41290790.52030-0.349603.051402.40830HepG2RECQL
BYSLENSG00000112578.5469.19340256.289500.872307.962406.98300HepG2RPS19
BYSLENSG00000112578.5394.47520534.40400-0.438002.032201.34670HepG2RPS3A
BYSLENSG00000112578.5295.46800396.47340-0.424202.935202.27630HepG2SAFB2
BYSLENSG00000112578.5816.19120486.293200.747005.894604.88610HepG2SF3A3
BYSLENSG00000112578.5538.44520665.42400-0.305502.695502.00090HepG2SLBP
BYSLENSG00000112578.5952.401801134.28040-0.252102.392901.91550HepG2SRFBP1
BYSLENSG00000112578.5838.67010685.241400.291402.295001.71800HepG2SRSF5
BYSLENSG00000112578.5465.56790622.87260-0.419903.534602.76150HepG2SRSF7
BYSLENSG00000112578.5575.22860984.82140-0.7757014.8195013.82160HepG2SSB
BYSLENSG00000112578.51121.43650779.343900.524907.976507.01100HepG2SUCLG1
BYSLENSG00000112578.5518.94350744.96190-0.521605.635004.88390HepG2TAF15
BYSLENSG00000112578.5211.84100432.63470-1.0302011.3798010.48670HepG2TIAL1
BYSLENSG00000112578.5576.87500429.347900.426002.480901.88400HepG2U2AF1
BYSLENSG00000112578.5559.25360355.219100.654704.655303.66670HepG2UCHL5
BYSLENSG00000112578.5830.05580416.372500.9952019.0792017.72560HepG2UTP3
BYSLENSG00000112578.5600.72930431.213200.478202.263301.30850HepG2XRCC6
BYSLENSG00000112578.5239.35290469.25890-0.9712012.8012011.52910HepG2ZC3H8
BYSLENSG00000112578.52064.819701626.978900.343702.167101.63660K562AQR
BYSLENSG00000112578.5331.14250469.48120-0.503603.266802.10170K562CCAR1
BYSLENSG00000112578.5314.78920440.15930-0.483603.043702.22250K562CPSF6
BYSLENSG00000112578.5461.29660816.87970-0.824409.521606.89870K562DDX19B
BYSLENSG00000112578.51832.958501060.195400.7897020.6834019.05530K562DDX47
BYSLENSG00000112578.51108.56200876.375000.339002.114801.31610K562EFTUD2
BYSLENSG00000112578.51139.333101359.25340-0.254602.408701.73000K562EIF2S2
BYSLENSG00000112578.5970.39900488.880500.9890018.3819016.26740K562EIF3G
BYSLENSG00000112578.5885.17390641.633000.464103.857202.89310K562EIF4A3
BYSLENSG00000112578.5367.64530542.81140-0.562103.609402.29530K562FKBP4
BYSLENSG00000112578.5257.79000447.84330-0.796806.521305.18660K562FMR1
BYSLENSG00000112578.51475.90380667.267301.1452032.1597030.22690K562FXR1
BYSLENSG00000112578.5173.49050375.85220-1.115305.385804.32380K562G3BP2
BYSLENSG00000112578.5396.61420625.67760-0.657705.534404.71910K562HNRNPC
BYSLENSG00000112578.5202.50950358.82970-0.825304.368003.16930K562KRR1
BYSLENSG00000112578.51158.66480703.020600.7207010.060608.38870K562MAGOH
BYSLENSG00000112578.51064.01070701.557500.600807.300405.70950K562MATR3
BYSLENSG00000112578.5785.488501003.22840-0.353004.441203.52080K562NSUN2
BYSLENSG00000112578.5829.30500625.470000.406903.011001.99320K562PCBP2
BYSLENSG00000112578.51435.54450852.817000.751209.195907.82430K562PRPF8
BYSLENSG00000112578.51335.91500683.046700.9677012.0265010.69530K562PTBP1
BYSLENSG00000112578.51618.949401934.11000-0.256602.299201.42250K562PUM2
BYSLENSG00000112578.5905.25300654.827700.467103.966902.94320K562RBM22
BYSLENSG00000112578.5934.19020689.132400.438803.346502.13140K562NELFE
BYSLENSG00000112578.51384.79480868.599500.672806.349005.09940K562RPS19
BYSLENSG00000112578.5481.82650316.753800.605102.394501.47090K562RPS3
BYSLENSG00000112578.51030.29300700.683500.556105.850104.28090K562SAFB
BYSLENSG00000112578.5557.68260390.616400.513604.263303.19690K562SF3B4
BYSLENSG00000112578.51052.08430669.640700.651708.099206.90000K562SRSF1
BYSLENSG00000112578.5731.41450904.44390-0.306302.900201.70020K562SRSF7
BYSLENSG00000112578.51113.79610788.760300.497705.668404.48540K562SYNCRIP
BYSLENSG00000112578.5199.63840368.92620-0.885903.067601.50130K562TAF15
BYSLENSG00000112578.5847.85980677.661400.323201.971601.36720K562TARDBP
BYSLENSG00000112578.5804.47400653.696400.299301.997401.30910K562TIA1
BYSLENSG00000112578.5369.44200539.56250-0.546403.410202.36620K562TIAL1
BYSLENSG00000112578.51145.738801368.12280-0.255902.664601.42040K562TROVE2
BYSLENSG00000112578.5732.963501063.74500-0.537308.429907.25870K562UPF1
BYSLENSG00000112578.5613.52810910.21180-0.569106.071904.74620K562WDR3
BYSLENSG00000112578.51163.20390946.523000.297303.430202.49540K562WDR43
BYSLENSG00000112578.51358.854801833.14860-0.431902.069201.33390K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for BYSL is not available on ENCORE database.





2 RBPs can affect the splicing of BYSL :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEBYSLENSG00000112578.5chr6+418978134189800841895068418952744189837241898506-0.077000.006180.05541excludedK562AQR
SEBYSLENSG00000112578.5chr6+418978694189800841895111418952744189837241898506-0.089000.007310.09263excludedK562HNRNPC
A3SSBYSLENSG00000112578.5chr6+4189908941899294418991334189929441898320418985060.073000.000000.00000longerK562AQR
A3SSBYSLENSG00000112578.5chr6+4189509641895274418951114189527441889271418895680.069000.000000.00000longerK562AQR
A3SSBYSLENSG00000112578.5chr6+4189944141899568418994654189956841899155418992940.083000.000000.00000longerK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for BYSL is not avaliable on ENCORE database.