BCAT2

General Information

Gene Name       : BCAT2
Gene ID             : ENSG00000105552.14
Essential Gene : No

Functions

Localization

Domains (AlphaFold: BCAT2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878BCAT2ENSG00000105552.149.7931.9720.88ENCSR000AEF
HepG2BCAT2ENSG00000105552.142.111.031.57ENCSR985KAT
K562BCAT2ENSG00000105552.1449.4036.2642.83ENCSR000AEO

Assays

0 Released experiments are available on ENCORE database.







  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
BCAT279764S1Submitted, ENCORE2BGKcLV42-9, Submitted, ENCORE2


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
BCAT2K562CRISPRBCAT2-BGKcLV42-9,BCAT2-BGKcLV42-10NT-BGKcLV42-1,NT-BGKcLV42-293.2,95.0SequencedENCORE2
BCAT2HepG2CRISPRBGHcLV43-5,BGHcLV43-6BGHcLV43-1,BGHcLV43-2NO BAND ON THE POSITION,NotSatisfiedENCORE2


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
BCAT2Product_ID: 79764S
Lot_ID: 1
Source: Cell Signaling Technology
Target Name: BCAT2-human		 CST_79764S_1_BCAT2.png<br>Caption: IP-WB analysis of 79764S whole cell lysate using the BCAT2 specific antibody, 79764S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the BCAT2-specific antibody, 79764S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 BCAT2-K562-CRISPR-79764S.png<br>Caption: Western blot following CRISPR against BCAT2 in K562 whole cell lysate using BCAT2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against BCAT2. BCAT2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for BCAT2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • BCAT2 RNA is bound by 13 proteins.  







eCLIP assay for BCAT2 is not avaliable on ENCORE database.





13 proteins are bound to BCAT2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr194929833749298369-3'-UTR,intron-Distal,intron-Proximal5.124499.39648K562PABPC4
chr194929853349298586-3'-UTR,intron-Distal3.275614.30970K562IGF2BP2
chr194929840749298458-3'-UTR,intron-Distal3.913867.26403K562IGF2BP2
chr194930971649309775-CDS,intron-Distal,intron-Proximal4.175179.03062K562AQR
chr194930014349300195-CDS,intron-Distal,intron-Proximal4.045393.12843K562PRPF8
chr194930297049303034-CDS,intron-Distal4.2045313.07366K562YBX3
chr194930019149300280-CDS,intron-Distal,intron-Proximal4.6017211.63297K562RPS3
chr194930303449303054-CDS,intron-Distal3.239358.40639K562YBX3
chr194930057049300633-CDS,intron-Distal,intron-Proximal5.6956910.47189K562SF3B4
chr194930354549303607-CDS,intron-Distal,intron-Proximal4.734826.99120K562AQR
chr194930287049302911-intron-Distal,intron-Proximal4.010923.31283K562PRPF8
chr194930852449308603-intron-Distal4.6453310.59779K562GTF2F1
chr194930880249308881-intron-Distal4.859774.92383K562FAM120A
chr194931020849310248-intron-Distal,intron-Proximal4.538676.99120K562AQR
chr194931024849310257-intron-Distal,intron-Proximal4.181375.47926K562AQR
chr194930936249309410-intron-Distal5.013615.84281K562GTF2F1
chr194930889549309008-intron-Distal5.337774.95161K562GTF2F1
chr194930882749308995-intron-Distal3.221454.70418K562AKAP8L
chr194931072049310780-intron-Distal4.506294.84986K562GTF2F1
chr194931384649313973-intron-Distal4.099268.06523K562GTF2F1
chr194930870549308769-intron-Distal4.5491510.10968K562GTF2F1
chr194930013949300193-intron-Distal,intron-Proximal4.885414.13847K562AQR
chr194931078049310828-intron-Distal3.986393.19125K562GTF2F1
chr194930880949308882-intron-Distal3.211803.40404K562CSTF2T
chr194930881149308895-intron-Distal4.2597511.53121K562GTF2F1
chr194931154849311630-intron-Distal,intron-Proximal3.660196.39777K562PTBP1
chr194931384049313944-intron-Distal4.821167.68230K562CSTF2T
chr194930824549308296-intron-Distal4.600604.40618K562PTBP1
chr194931071349310886-intron-Distal3.465923.96485K562AKAP8L
chr194930855849308627-intron-Distal3.932093.59342K562CSTF2T
chr194931208449312151-intron-Distal4.556403.41592K562XRCC6
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for BCAT2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for BCAT2 is not available on ENCORE database.

  • 62 RBPs can affect the levels of BCAT2.  








The Knockdown/knockout RNA-Seq assay for BCAT2 is not available on ENCORE database.





62 RBPs can affect the gene expression of BCAT2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
BCAT2ENSG00000105552.1017.8025053.33940-1.583104.165503.44790HepG2ACO1
BCAT2ENSG00000105552.1039.3946078.86140-1.001302.676502.00070HepG2AUH
BCAT2ENSG00000105552.1048.4063021.076001.199502.495701.53220HepG2YBX3
BCAT2ENSG00000105552.1057.6319020.687101.478003.878003.05740HepG2DNAJC21
BCAT2ENSG00000105552.1043.8884020.515901.097002.220501.62380HepG2DNAJC2
BCAT2ENSG00000105552.1015.5505052.53190-1.756204.123602.70700HepG2EIF2S2
BCAT2ENSG00000105552.10129.3293063.229501.032303.814003.06870HepG2EIF4A3
BCAT2ENSG00000105552.1052.9502020.885401.342002.677502.04020HepG2HNRNPA1
BCAT2ENSG00000105552.1046.3397023.081101.005401.910601.39790HepG2HNRNPU
BCAT2ENSG00000105552.1059.4522027.982101.087102.184401.53690HepG2IGF2BP1
BCAT2ENSG00000105552.1051.9861023.575901.140702.726001.99820HepG2ILF2
BCAT2ENSG00000105552.1030.9053065.69880-1.088002.385101.79460HepG2NCBP2
BCAT2ENSG00000105552.1029.1899064.82050-1.151002.927502.32140HepG2PUM1
BCAT2ENSG00000105552.107.4165042.53500-2.519804.246703.38270HepG2RAVER1
BCAT2ENSG00000105552.1034.8170068.25780-0.971202.320301.69740HepG2RBM25
BCAT2ENSG00000105552.1024.1728055.53250-1.199902.488201.84950HepG2SF3A3
BCAT2ENSG00000105552.1041.4353085.47070-1.044602.924102.41340HepG2SRFBP1
BCAT2ENSG00000105552.1031.6946075.46560-1.251603.478302.96380HepG2SRSF1
BCAT2ENSG00000105552.1064.9414022.629001.520904.757504.05850HepG2TRIM56
BCAT2ENSG00000105552.1020.3491059.81880-1.555603.689602.96800HepG2U2AF1
BCAT2ENSG00000105552.1061.5739025.922601.248002.145501.50010HepG2XRN2
BCAT2ENSG00000105552.1019.1371044.57720-1.219902.376101.74820HepG2ZC3H8
BCAT2ENSG00000105552.10510.63680719.99250-0.495702.966701.73640K562AARS
BCAT2ENSG00000105552.10623.03540871.86820-0.484803.269501.92460K562ABCF1
BCAT2ENSG00000105552.101800.604701074.438600.7448018.7230017.36970K562AGGF1
BCAT2ENSG00000105552.101493.231001180.636300.338802.838901.84060K562AKAP8
BCAT2ENSG00000105552.10813.535601275.17390-0.648407.089606.22090K562AQR
BCAT2ENSG00000105552.10827.29510644.505300.360103.214202.18030K562BCLAF1
BCAT2ENSG00000105552.10526.95890943.11600-0.839705.495603.93690K562CNOT7
BCAT2ENSG00000105552.10699.76290488.953500.517104.705003.27370K562CSTF2
BCAT2ENSG00000105552.10734.634201182.84500-0.6872012.9445011.64500K562DDX47
BCAT2ENSG00000105552.101479.909501203.062300.298703.204102.25290K562DKC1
BCAT2ENSG00000105552.10352.15480957.86090-1.4436038.0270035.97450K562EIF2S2
BCAT2ENSG00000105552.10302.80500640.93500-1.081807.519505.81790K562EIF3A
BCAT2ENSG00000105552.101333.386601638.91070-0.297603.686602.60260K562EIF4G2
BCAT2ENSG00000105552.10867.11000626.830200.468003.888402.87300K562FIP1L1
BCAT2ENSG00000105552.10432.84840684.35570-0.660902.840301.69660K562FKBP4
BCAT2ENSG00000105552.10660.87250948.12150-0.520706.438405.57130K562FXR1
BCAT2ENSG00000105552.1064.32150310.27330-2.2702011.035209.43460K562G3BP2
BCAT2ENSG00000105552.10417.49230787.24450-0.915107.600706.65770K562HNRNPC
BCAT2ENSG00000105552.10993.00310541.926900.873603.086601.98210K562IGF2BP2
BCAT2ENSG00000105552.101680.027801329.162000.337903.049602.08660K562MBNL1
BCAT2ENSG00000105552.10895.48250566.765100.659802.536601.31400K562NONO
BCAT2ENSG00000105552.10795.621501143.55370-0.523404.189303.16470K562PCBP3
BCAT2ENSG00000105552.10637.59400877.12880-0.460204.315803.31090K562POLR2G
BCAT2ENSG00000105552.101002.87220711.656100.494803.581302.62810K562PPIG
BCAT2ENSG00000105552.10935.84590705.917300.406703.086302.12520K562PSIP1
BCAT2ENSG00000105552.10874.16770552.779800.661103.629602.88090K562PTBP1
BCAT2ENSG00000105552.101291.992201788.12080-0.468804.899203.81810K562PUF60
BCAT2ENSG00000105552.101500.011601902.33330-0.342804.158802.83320K562PUM1
BCAT2ENSG00000105552.101627.497601108.765600.553606.281805.50250K562PUS1
BCAT2ENSG00000105552.101437.752301080.311300.412304.945504.17390K562RBM15
BCAT2ENSG00000105552.10478.20980314.587000.604104.438802.17760K562RECQL
BCAT2ENSG00000105552.10297.12020569.19140-0.937903.110402.24240K562RPS19
BCAT2ENSG00000105552.10570.81450841.29750-0.559602.959101.31370K562RPS3A
BCAT2ENSG00000105552.10270.19500412.62080-0.610802.445301.53520K562SF3B1
BCAT2ENSG00000105552.101345.91130801.560100.747603.225001.78010K562SFPQ
BCAT2ENSG00000105552.10800.83890610.835000.390602.654901.65710K562SRFBP1
BCAT2ENSG00000105552.10966.811201133.42500-0.229402.131901.30130K562SRPK2
BCAT2ENSG00000105552.10874.74290541.979600.690502.379001.71550K562SRSF1
BCAT2ENSG00000105552.10853.61700512.034700.737204.672803.24470K562SRSF5
BCAT2ENSG00000105552.10467.63360840.79720-0.846404.232502.20500K562STIP1
BCAT2ENSG00000105552.10896.07520529.000700.760304.542103.45550K562TIA1
BCAT2ENSG00000105552.10825.33440519.469500.667803.004201.85760K562TRA2A
BCAT2ENSG00000105552.10172.81020310.34890-0.844703.758902.82130K562U2AF2
BCAT2ENSG00000105552.10836.018601057.75870-0.339403.986802.98390K562WDR43
BCAT2ENSG00000105552.101173.343501436.40830-0.291802.143801.39450K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for BCAT2 is not available on ENCORE database.





5 RBPs can affect the splicing of BCAT2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEBCAT2ENSG00000105552.10chr19-493097734930997449303443493035544931424049314284-0.236000.004950.07532excludedK562SRSF1
SEBCAT2ENSG00000105552.10chr19-493102564931033149303443493035544931424049314284-0.288000.000480.02111excludedK562PRPF8
SEBCAT2ENSG00000105552.10chr19-493102564931033149303443493035544931424049314284-0.268000.000180.00991excludedK562TARDBP
A3SSBCAT2ENSG00000105552.10chr19-4930344349303651493034434930355449309773493099670.113000.000060.00054longerK562AQR
MXEBCAT2ENSG00000105552.10chr19-49309773493099744931025649310331493034434930355449314240493142840.230000.003210.084751includedK562LIN28B

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for BCAT2 is not avaliable on ENCORE database.