IGF2BP1

General Information

Gene Name : IGF2BP1
Gene ID : ENSG00000159217.9
Essential Gene : No

Functions

RNA stability & decay
RNA localization
Translation regulation
Viral RNA regulation

Localization(RBPImage: IGF2BP1)

Nuclei
Nucleoplasm
Cajal bodies
Cytoplasm
Cytosolic
Mitotic-Apparatus (Spindle; Centrosomes)

Domains (AlphaFold: IGF2BP1)

RRM
KH

Antibody Validation

View validation images for all IGF2BP1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	IGF2BP1	ENSG00000159217.9	0.11	0.10	0.11	ENCSR000AEF
HepG2	IGF2BP1	ENSG00000159217.9	73.56	74.01	73.78	ENCSR985KAT
K562	IGF2BP1	ENSG00000159217.9	51.86	54.07	52.97	ENCSR000AEO

Assays

7 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 205	ENCSR744GEU	IGF2BP1	HepG2	Released	ENCAB108MXA	ENCSR205ZCE
eCLIP	eCLIP: 220	ENCSR975KIR	IGF2BP1	K562	Released	ENCAB108MXA	ENCSR125CLF
knockdown followed by RNA-seq	IGF2BP1-BGKLV11-K562	ENCSR629EWX	IGF2BP1	K562	Released		ENCSR129RWD	shRNA	KD% = 68.0,70.2
knockdown followed by RNA-seq	IGF2BP1-BGHLV14-HepG2	ENCSR708GKW	IGF2BP1	HepG2	Released		ENCSR491FOC	shRNA	KD% = 73.6,60.0
knockdown followed by RNA-seq	IGF2BP1-BGKcLV02-K562	ENCSR543LCG	IGF2BP1	K562	Released		ENCSR163JUC	CRISPR	KD% = 92.2,91.2
knockdown followed by RNA-seq	IGF2BP1-BGHcLV03-HepG2	ENCSR268XHM	IGF2BP1	HepG2	Released		ENCSR105NML	CRISPR	KD% = 91.5,90.8
RNA Bind-n-Seq	RBNS: IGF2BP1	ENCSR928XOW	IGF2BP1	cell-free sample	Released

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
IGF2BP1	205	HepG2	ENCSR744GEU	ENCSR205ZCE	ENCAB108MXA	RN007P	3	Released	ENCODE3
IGF2BP1	220	K562	ENCSR975KIR	ENCSR125CLF	ENCAB108MXA	RN007P	3	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
IGF2BP1	ENCAB108MXA	RN007P	3		Released, ENCODE3	Released, ENCODE3	BGHLV12-39, Released, ENCODE3	BGKcLV02-3, Released, ENCODE3
IGF2BP1		8482S	2		Released, ENCODE3	Submitted, ENCORE2	BGHLV12-39, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
IGF2BP1	K562	shRNA	ENCSR629EWX	ENCSR129RWD	IGF2BP1-LV11-17,IGF2BP1-LV11-18	Nontarget-LV11-1,nontarget-LV11-2	68.0,70.2	Released	ENCODE3
IGF2BP1	HepG2	shRNA			BGHLV12-39,BGHLV12-40	BGHLV12-1,BGHLV12-2		NotSatisfied	ENCODE3
IGF2BP1	HepG2	shRNA	ENCSR708GKW	ENCSR491FOC	IGF2BP1_BGHLV14-27,IGF2BP1_BGHLV14-28	NT_BGHLV14-1,NT_BGHLV14-2	73.6,60.0	Released	ENCODE3
IGF2BP1	K562	CRISPR	ENCSR543LCG	ENCSR163JUC	IGF2BP1_BGKcLV02-3,IGF2BP1_BGKcLV02-4	NT_BGKcLV02-1,NT_BGKcLV02-2	92.2,91.2	Released	ENCODE3
IGF2BP1	K562	CRISPR			BGKcLV44-101,BGKcLV44-102	BGKcLV44-1,BGKcLV44-2	54.6,59.0	NotSatisfied	ENCORE2
IGF2BP1	HepG2	CRISPR	ENCSR268XHM	ENCSR105NML	IGF2BP1_BGHcLV03-3,IGF2BP1_BGHcLV03-4	NT_BGHcLV03-1,NT_BGHcLV03-2	91.5,90.8	Released	ENCODE3

RNA Bind-n-Seqn

GeneName	gene_aliases	Status	Project	Note
IGF2BP1	IGF2BP1		Released	ENCODE3

Localization Image in HepG2

IGF2BP1: Click to RBP Image Database

Nuclei

Nucleoplasm

Cajal bodies

Cytoplasm

Cytosolic

Mitotic-Apparatus (Spindle; Centrosomes)

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
IGF2BP1	Product_ID: RN007P Lot_ID: 3 Source: MBLI Target Name: IGF2BP1-human
IGF2BP1	Product_ID: 8482S Lot_ID: 2 Source: Cell Signaling Technology Target Name: IGF2BP1-human

RNA Targets

IGF2BP1 protein binds to 9,986 RNA regions.

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The binding motifs of IGF2BP1

Proteins bound to RNA

IGF2BP1 RNA is bound by 62 proteins.

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IGF2BP1 protein has 9,986 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
IGF2BP1	chr8	101721688	101721696	-	4.84662	83.57324	HepG2	PABPC1	3'-UTR,CDS,intron-Distal
IGF2BP1	chr2	21224511	21224518	-	4.24410	71.30627	HepG2	APOB	3'-UTR,intron-Distal
IGF2BP1	chr2	21224547	21224555	-	4.32801	60.77309	HepG2	APOB	3'-UTR,intron-Distal
IGF2BP1	chr12	9232284	9232294	-	5.10770	46.70051	HepG2	A2M	CDS,intron-Distal
IGF2BP1	chr5	32125966	32126038	-	5.11863	34.81967	K562	GOLPH3	3'-UTR,intron-Distal
IGF2BP1	chr1	160259751	160259810	-	5.60134	27.34254	K562	COPA	3'-UTR,intron-Distal
IGF2BP1	chr7	135611611	135611630	-	4.63273	25.41354	K562	MTPN	3'-UTR,intron-Distal
IGF2BP1	chr13	30087318	30087358	-	4.64138	24.86924	HepG2	SLC7A1	3'-UTR,intron-Distal
IGF2BP1	chr16	30735078	30735134	+	3.71674	22.28290	HepG2	SRCAP	CDS
IGF2BP1	chr2	128948212	128948287	+	4.74549	19.56853	HepG2	UGGT1	3'-UTR

Showing 1 to 10 of 100 entries

62 proteins are bound to IGF2BP1 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	47126799	47126886	+	3'-UTR,CDS	7.23833	400.00000	HepG2	UPF1
chr17	47126947	47127003	+	3'-UTR	6.57581	400.00000	K562	UPF1
chr17	47127633	47127646	+	3'-UTR	5.18492	400.00000	K562	UPF1
chr17	47127530	47127558	+	3'-UTR	5.12947	400.00000	K562	UPF1
chr17	47127719	47127781	+	3'-UTR	6.04043	400.00000	K562	UPF1
chr17	47127627	47127699	+	3'-UTR	5.99912	400.00000	K562	AKAP1
chr17	47127602	47127633	+	3'-UTR	4.71934	400.00000	K562	UPF1
chr17	47126886	47126921	+	3'-UTR	7.47262	400.00000	HepG2	UPF1
chr17	47126870	47126947	+	3'-UTR	5.94397	400.00000	K562	AKAP1
chr17	47126882	47126907	+	3'-UTR	5.50812	400.00000	K562	UPF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 354 entries

The IGF2BP1 protein binds to its own RNA.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
IGF2BP1	chr17	47077286	47077381	+	3.42446	3.57062	K562	IGF2BP1	intron-Distal
IGF2BP1	chr17	47131743	47131787	+	4.02990	3.54770	K562	IGF2BP1	3'-UTR

Showing 1 to 2 of 2 entries

Diffrential Expression

6,140 differentially expressed genes after Knockdown/Knockout IGF2BP1 protein.

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129 RBPs can affect the levels of IGF2BP1.

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6,140 differentially expressed genes after Knockdown/Knockout IGF2BP1 protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
MLXIPL	ENSG00000009950.11	6457.02180	1920.47170	1.74930	88.23900	85.30220	HepG2	IGF2BP1
CRLF1	ENSG00000006016.6	1466.55310	4300.36640	-1.55200	60.17390	57.60280	HepG2	IGF2BP1
ZFX	ENSG00000005889.11	1595.79330	585.86170	1.44550	41.92810	39.65800	HepG2	IGF2BP1
ASB4	ENSG00000005981.8	31.38430	275.67380	-3.13480	39.02440	36.80180	HepG2	IGF2BP1
PLEKHG6	ENSG00000008323.11	619.47460	180.47560	1.77910	36.05330	33.89720	HepG2	IGF2BP1
FYN	ENSG00000010810.13	443.67720	1008.77970	-1.18500	23.69120	21.82180	HepG2	IGF2BP1
MASP2	ENSG00000009724.12	490.72330	183.75990	1.41700	21.24160	19.45420	HepG2	IGF2BP1
CX3CL1	ENSG00000006210.6	3907.12140	2207.18530	0.82380	18.36760	16.69200	HepG2	IGF2BP1
CYP51A1	ENSG00000001630.11	3583.78940	2107.07790	0.76610	17.78400	16.12750	HepG2	IGF2BP1
ACSM3	ENSG00000005187.7	1189.51650	612.56970	0.95730	17.51470	15.87190	HepG2	IGF2BP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

129 RBPs can affect the gene expression of IGF2BP1 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
IGF2BP1	ENSG00000159217.5	11849.24010	5348.33680	1.14750	91.32960	89.50530	HepG2	SRSF1
IGF2BP1	ENSG00000159217.5	4076.97140	8372.76160	-1.03820	81.15440	78.59540	HepG2	ZC3H8
IGF2BP1	ENSG00000159217.5	3697.03230	10234.33800	-1.46900	72.69190	69.95140	HepG2	IGF2BP1
IGF2BP1	ENSG00000159217.5	5763.45920	9505.64430	-0.72180	47.55290	46.00920	HepG2	ZNF622
IGF2BP1	ENSG00000159217.5	2135.17800	7741.44720	-1.85820	49.88920	45.48060	K562	IGF2BP1
IGF2BP1	ENSG00000159217.5	9902.46260	5477.26480	0.85420	43.57500	42.00440	HepG2	NELFE
IGF2BP1	ENSG00000159217.5	3778.96380	6355.25820	-0.75000	38.22370	36.25130	HepG2	SAFB2
IGF2BP1	ENSG00000159217.5	8206.00320	4921.66100	0.73740	31.08610	29.65100	HepG2	PTBP1
IGF2BP1	ENSG00000159217.5	6008.03110	9179.49570	-0.61150	31.07300	29.09910	HepG2	DAZAP1
IGF2BP1	ENSG00000159217.5	7942.77420	4836.75110	0.71550	30.30870	28.66980	HepG2	PCBP1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 158 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

149 alternative splicing events after Knockdown/Knockout IGF2BP1 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SNRNP70	ENSG00000104852.10	chr19	+	49605370	49606844	49604646	49604728	49607890	49607992	0.09700	0.00010	included	HepG2	IGF2BP1
SE	SEC31A	ENSG00000138674.12	chr4	-	83752089	83752128	83750152	83750211	83763292	83763634	0.28900	0.00000	included	HepG2	IGF2BP1
SE	ZNF207	ENSG00000010244.12	chr17	+	30693683	30693776	30692348	30692506	30694790	30694962	0.14000	0.00081	included	HepG2	IGF2BP1
SE	KHK	ENSG00000138030.8	chr2	+	27315199	27315316	27309661	27310219	27319596	27319669	0.29200	0.00027	included	HepG2	IGF2BP1
SE	HNMT	ENSG00000150540.9	chr2	+	138724666	138724956	138722048	138722198	138727734	138727787	0.08600	0.00550	included	HepG2	IGF2BP1
SE	ATG12	ENSG00000145782.8	chr5	-	115176193	115176309	115173324	115173461	115177086	115177534	0.07100	0.00948	included	HepG2	IGF2BP1
SE	F2	ENSG00000180210.10	chr11	+	46750929	46751111	46750213	46750387	46760597	46760668	0.05500	0.00545	included	HepG2	IGF2BP1
SE	SRSF3	ENSG00000112081.12	chr6	+	36567597	36568053	36566625	36566760	36568928	36568967	0.06500	0.00550	included	HepG2	IGF2BP1
SE	SRSF7	ENSG00000115875.14	chr2	-	38976039	38976488	38975720	38975795	38976670	38976847	0.17500	0.00000	included	HepG2	IGF2BP1
SE	AHSA2	ENSG00000173209.18	chr2	+	61410681	61410797	61408472	61408540	61411846	61411935	-0.12400	0.00008	excluded	HepG2	IGF2BP1

Showing 1 to 10 of 100 entries

3 RBPs can affect the splicing of IGF2BP1 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A3SS	IGF2BP1	ENSG00000159217.5	chr17	+	47109232	47109335			47109271	47109335	47103827	47103879	0.06000	0.00000	0.00000	longer	K562	AQR
MXE	IGF2BP1	ENSG00000159217.5	chr17	+	47115529	47115811	47117318	47117453	47109271	47109335	47118739	47118862	0.12200	0.00061	0.07838	1included	HepG2	IGF2BP1
MXE	IGF2BP1	ENSG00000159217.5	chr17	+	47109271	47109335	47115529	47115811	47103827	47103879	47117318	47117453	-0.12200	0.00396	0.07151	2included	HepG2	DDX55

Showing 1 to 3 of 3 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The IGF2BP1 protein affects to its own RNA splicing after knockdown/knockout.

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP	Bound by RBP
MXE	IGF2BP1	ENSG00000159217.5	chr17	+	47115529	47115811	47117318	47117453	47109271	47109335	47118739	47118862	0.12200	0.00061	0.07838	1included	HepG2	IGF2BP1

Showing 1 to 1 of 1 entries