DDX1

General Information

Gene Name : DDX1
Gene ID : ENSG00000079785.14
Essential Gene : Yes

Functions

tRNA regulation
Viral RNA regulation

Localization(RBPImage: DDX1)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Compartment/Organelle
Cytoskeleton
Microtubule
Cytosolic

Domains (AlphaFold: DDX1)

Helicase

Antibody Validation

View validation images for all DDX1 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	DDX1	ENSG00000079785.14	69.31	66.41	67.86	ENCSR000AEF
HepG2	DDX1	ENSG00000079785.14	110.81	183.11	146.96	ENCSR985KAT
K562	DDX1	ENSG00000079785.14	156.72	161.03	158.88	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 4231	ENCSR293FWY	DDX1	K562	Released	ENCAB741VRO	ENCSR561QXJ
knockdown followed by RNA-seq	DDX1-BGKLV24-K562	ENCSR208GPE	DDX1	K562	Released		ENCSR667PLJ	shRNA	KD% = 77.3,75.0
knockdown followed by RNA-seq	DDX1-BGHLV20-HepG2	ENCSR070LJO	DDX1	HepG2	Released		ENCSR305XWT	shRNA	KD% = 82.0,72.6

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
DDX1	4231	K562	ENCSR293FWY	ENCSR561QXJ	ENCAB741VRO	703141	Unknown	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
DDX1	ENCAB297EGB	GTX105205	39904				BGHLV20-11, Released, ENCODE3	BGKLV24-13, Released, ENCODE3
DDX1	ENCAB741VRO	703141	None			Released, ENCODE4	BGHLV20-11, Released, ENCODE3	BGKLV24-13, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
DDX1	K562	shRNA	ENCSR208GPE	ENCSR667PLJ	DDX1_BGKLV24_13_17604,DDX1_BGKLV24_14_17605	non-target_BGKLV24_1_17594,non-target_BGKLV24_2_17595	77.3,75.0	Released	ENCODE3
DDX1	HepG2	shRNA	ENCSR070LJO	ENCSR305XWT	DDX1-BGHLV20-11_17712,DDX1-BGHLV20-12_17713	non-target-BGHLV20-1_17706,non-target-BGHLV20-2_17707	82.0,72.6	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

DDX1: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Compartment/Organelle

Cytoskeleton

Microtubule

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DDX1	Product_ID: 703141 Lot_ID: None Source: Thermo Fisher Target Name: DDX1-human
DDX1	Product_ID: GTX105205 Lot_ID: 39904 Source: GeneTex Target Name: DDX1-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for DDX1 is not avaliable on ENCORE database.

Proteins bound to RNA

DDX1 RNA is bound by 31 proteins.

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eCLIP assay for DDX1 is not avaliable on ENCORE database.

31 proteins are bound to DDX1 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr2	15771039	15771122	+	3'-UTR	6.47157	24.15382	HepG2	SUB1
chr2	15771122	15771174	+	3'-UTR	7.08692	21.85814	HepG2	SUB1
chr2	15744570	15744632	+	CDS	4.36938	18.33118	HepG2	BCLAF1
chr2	15732022	15732136	+	5'-UTR,CDS,intron-Proximal	3.84775	16.51596	HepG2	PRPF8
chr2	15739816	15739853	+	CDS,intron-Proximal	6.63287	14.11848	HepG2	PRPF8
chr2	15737696	15737790	+	intron-Distal	5.00044	13.76505	K562	PRPF8
chr2	15761203	15761264	+	CDS,intron-Proximal	4.89251	13.40522	HepG2	PRPF8
chr2	15736826	15736895	+	CDS,intron-Proximal	5.89382	12.95646	HepG2	AQR
chr2	15768864	15768983	+	CDS,intron-Proximal	3.65818	12.86780	K562	EFTUD2
chr2	15766258	15766331	+	intron-Distal	4.70361	11.12558	HepG2	QKI
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 117 entries

eCLIP assay for DDX1 is not avaliable on ENCORE database.

Diffrential Expression

2,094 differentially expressed genes after Knockdown/Knockout DDX1 protein.

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104 RBPs can affect the levels of DDX1.

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2,094 differentially expressed genes after Knockdown/Knockout DDX1 protein :

Only display 100 entries as example. Please click "View All" button to see all 2,094 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
CX3CL1	ENSG00000006210.6	2288.29390	1247.42180	0.87520	23.92260	21.16920	HepG2	DDX1
CP	ENSG00000047457.9	15416.26810	24241.55670	-0.65300	22.36550	19.71760	HepG2	DDX1
TAB2	ENSG00000055208.13	1227.51000	2064.25330	-0.74990	17.21260	14.79170	HepG2	DDX1
VCAN	ENSG00000038427.11	2327.27610	3654.01090	-0.65080	16.28440	13.90810	HepG2	DDX1
FAM168A	ENSG00000054965.6	640.66430	1076.35580	-0.74850	12.20680	10.04440	HepG2	DDX1
SCML1	ENSG00000047634.10	3055.64440	2078.47200	0.55590	11.78690	9.65420	HepG2	DDX1
CYP51A1	ENSG00000001630.11	1736.98340	2708.20120	-0.64080	11.59310	9.47820	HepG2	DDX1
UBE3C	ENSG00000009335.13	1820.30950	1194.13860	0.60810	11.27280	9.19380	HepG2	DDX1
IFNGR1	ENSG00000027697.8	1376.49190	2150.57530	-0.64370	10.78580	8.74840	HepG2	DDX1
CD74	ENSG00000019582.10	1920.36870	1285.39440	0.57910	10.66470	8.63140	HepG2	DDX1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

104 RBPs can affect the gene expression of DDX1 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
DDX1	ENSG00000079785.10	423.89770	2877.43680	-2.76300	181.40170	177.04630	HepG2	DDX1
DDX1	ENSG00000079785.10	236.41090	2143.20270	-3.18040	66.45990	62.11280	K562	DDX1
DDX1	ENSG00000079785.10	1160.38750	3058.28570	-1.39810	53.81890	52.13910	HepG2	TIAL1
DDX1	ENSG00000079785.10	3015.99890	6281.24490	-1.05840	49.50330	46.73980	K562	SF3A3
DDX1	ENSG00000079785.10	2186.76600	3404.12900	-0.63850	25.96110	24.71560	HepG2	ZNF622
DDX1	ENSG00000079785.10	1776.98940	2802.98180	-0.65750	21.81270	20.25030	HepG2	SAFB2
DDX1	ENSG00000079785.10	3302.64860	5109.83860	-0.62970	21.54260	20.06990	K562	AGGF1
DDX1	ENSG00000079785.10	1819.38600	2796.64630	-0.62020	21.37780	19.71980	HepG2	DNAJC2
DDX1	ENSG00000079785.10	5191.44250	8205.12900	-0.66040	21.24780	19.53500	K562	EIF2S2
DDX1	ENSG00000079785.10	2784.25020	4948.26360	-0.82960	18.92610	17.55370	HepG2	PUF60
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 128 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for DDX1 is not available on ENCORE database.

12 RBPs can affect the splicing of DDX1 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	DDX1	ENSG00000079785.10	chr2	+	15753347	15753408			15747298	15747437	15757367	15757466	-0.07100	0.00000	0.00000	excluded	K562	AQR
SE	DDX1	ENSG00000079785.10	chr2	+	15768868	15768963			15768568	15768641	15769725	15769821	-0.09200	0.00000	0.00000	excluded	K562	AQR
SE	DDX1	ENSG00000079785.10	chr2	+	15768920	15768963			15768568	15768641	15769725	15769821	-0.09700	0.00000	0.00000	excluded	HepG2	SF3A3
SE	DDX1	ENSG00000079785.10	chr2	+	15768920	15768963			15767177	15767269	15769725	15769821	-0.08200	0.00000	0.00000	excluded	K562	TARDBP
A3SS	DDX1	ENSG00000079785.10	chr2	+	15757334	15757466			15757367	15757466	15753347	15753408	0.06900	0.00000	0.00000	longer	K562	AQR
MXE	DDX1	ENSG00000079785.10	chr2	+	15747243	15747437	15753347	15753408	15746273	15746388	15757367	15757466	0.11800	0.00000	0.00000	1included	K562	AQR
SE	DDX1	ENSG00000079785.10	chr2	+	15753347	15753408			15747298	15747437	15757367	15757466	-0.08800	0.00001	0.00149	excluded	HepG2	PRPF8
SE	DDX1	ENSG00000079785.10	chr2	+	15768761	15768834			15768568	15768641	15769725	15769821	-0.07600	0.00003	0.00244	excluded	K562	PRPF8
MXE	DDX1	ENSG00000079785.10	chr2	+	15747298	15747437	15753347	15753408	15746273	15746388	15757367	15757466	0.11400	0.00004	0.00569	1included	K562	PHF6
RI	DDX1	ENSG00000079785.10	chr2	+	15768568	15768812			15768568	15768641	15768761	15768812	0.06200	0.00005	0.00117	included	HepG2	SF3A3

Showing 1 to 10 of 22 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

DDX1

General Information

Functions

Localization(RBPImage: DDX1)

Domains (AlphaFold: DDX1)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for DDX1 is not avaliable on ENCORE database.

31 proteins are bound to DDX1 RNA:

eCLIP assay for DDX1 is not avaliable on ENCORE database.

Diffrential Expression

2,094 differentially expressed genes after Knockdown/Knockout DDX1 protein :

104 RBPs can affect the gene expression of DDX1 :

The Knockdown/knockout RNA-Seq assay for DDX1 is not available on ENCORE database.

12 RBPs can affect the splicing of DDX1 :

The Knockdown/knockout RNA-Seq assay for DDX1 is not avaliable on ENCORE database.