SRSF4

General Information

Gene Name : SRSF4
Gene ID : ENSG00000116350.16
Essential Gene : No

Functions

Splicing regulation
Viral RNA regulation

Localization(RBPImage: SRSF4)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Speckles
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: SRSF4)

RRM

Antibody Validation

View validation images for all SRSF4 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SRSF4	ENSG00000116350.16	68.55	73.04	70.80	ENCSR000AEF
HepG2	SRSF4	ENSG00000116350.16	61.89	71.31	66.60	ENCSR985KAT
K562	SRSF4	ENSG00000116350.16	83.50	88.67	86.09	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Controls	Method	Note
knockdown followed by RNA-seq	SRSF4-BGKLV13-K562	ENCSR697GLD	SRSF4	K562	Released	ENCSR815CVQ	shRNA	KD% = 25.9,11.5
knockdown followed by RNA-seq	SRSF4-BGKcLV04-K562	ENCSR939BTN	SRSF4	K562	Released	ENCSR005JBU	CRISPR	KD% = 66,73.8
knockdown followed by RNA-seq	SRSF4-BGHcLV05-HepG2	ENCSR929JKA	SRSF4	HepG2	Released	ENCSR805TIZ	CRISPR	KD% = 54.8,57.2
RNA Bind-n-Seq	RBNS: SRSF4	ENCSR252RIJ	SRSF4	cell-free sample	Released

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SRSF4	ENCAB653SNR	A303-670A	1		Released, ENCODE3	Released, ENCODE3		BGKcLV04-29, Released, ENCODE3
SRSF4	ENCAB000AXC	ARP40591_P050	QC21851		Released, ENCODE3	Released, ENCODE3		BGKcLV04-29, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
SRSF4	K562	shRNA	ENCSR697GLD	ENCSR815CVQ	SRSF4_BGKLV13_49,SRSF4_BGKLV13_50	NT_BGKLV13_1,NT_BGKLV13_2	25.9,11.5	Released	ENCODE3
SRSF4	HepG2	shRNA			BGHLV14-53,BGHLV14-54	BGHLV14-1,BGHLV14-2		NotSatisfied	ENCODE3
SRSF4	K562	CRISPR	ENCSR939BTN	ENCSR005JBU	SRSF4-BGKcLV04-29C,SRSF4-BGKcLV04-30C	NT-BGKcLV04-1C,NT-BGKcLV04-2C	66,73.8	Released	ENCODE3
SRSF4	K562	CRISPR			BGKcLV04-29-D12,BGKcLV04-30-D12	BGKcLV04-1,BGKcLV04-2		NotSatisfied	ENCODE3
SRSF4	K562	CRISPR			BGKcLV04-30-D12,BGKcLV04-29-D20	BGKcLV04-1,BGKcLV04-2		NotSatisfied	ENCODE3
SRSF4	K562	CRISPR			BGKcLV04-29-D20,BGKcLV04-30-D20	BGKcLV04-1,BGKcLV04-2		NotSatisfied	ENCODE3
SRSF4	K562	CRISPR			BGKcLV04-30-D20,BGHcLV05-33	BGKcLV04-1,BGKcLV04-2	,54.8	NotSatisfied	ENCODE3
SRSF4	HepG2	CRISPR	ENCSR929JKA	ENCSR805TIZ	SRSF4-BGHcLV05-33D,SRSF4-BGHcLV05-34D	NT-BGHcLV05-1D,NT-BGHcLV05-2D	54.8,57.2	Released	ENCODE3
SRSF4	HepG2	CRISPR			BGHcLV09-19,BGHcLV09-20	BGHcLV09-1,BGHcLV09-2		NotSatisfied	ENCODE4

RNA Bind-n-Seqn

GeneName	gene_aliases	Status	Project	Note
SRSF4	SRSF4		Released	ENCODE3

Localization Image in HepG2

SRSF4: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Speckles

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SRSF4	Product_ID: A303-670A Lot_ID: 1 Source: Bethyl Labs Target Name: SRSF4-human
SRSF4	Product_ID: ARP40591_P050 Lot_ID: QC21851 Source: Aviva Target Name: SRSF4-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for SRSF4 is not avaliable on ENCORE database.

The binding motifs of SRSF4

Proteins bound to RNA

SRSF4 RNA is bound by 63 proteins.

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eCLIP assay for SRSF4 is not avaliable on ENCORE database.

63 proteins are bound to SRSF4 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr1	29474825	29474857	-	3'-UTR,intron-Distal	6.08437	400.00000	K562	PUM2
chr1	29474738	29474825	-	3'-UTR,intron-Distal	4.86569	400.00000	K562	PUM2
chr1	29474286	29474334	-	3'-UTR,intron-Distal	5.12131	400.00000	K562	PABPC4
chr1	29474861	29474919	-	3'-UTR,intron-Distal	5.66431	400.00000	K562	PUM2
chr1	29475436	29475509	-	3'-UTR,CDS,intron-Distal	5.81064	78.86688	K562	UCHL5
chr1	29475415	29475436	-	3'-UTR,CDS,intron-Distal	6.64037	76.89408	K562	UCHL5
chr1	29474425	29474473	-	3'-UTR,intron-Distal	4.17960	65.49790	K562	PUM2
chr1	29475394	29475415	-	3'-UTR,CDS,intron-Distal	7.55408	49.71399	K562	UCHL5
chr1	29508235	29508331	-	5'-UTR,CDS,intron-Distal	4.16413	46.62843	K562	GEMIN5
chr1	29475166	29475255	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.22591	46.51475	K562	UCHL5
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 202 entries

eCLIP assay for SRSF4 is not avaliable on ENCORE database.

Diffrential Expression

2,946 differentially expressed genes after Knockdown/Knockout SRSF4 protein.

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84 RBPs can affect the levels of SRSF4.

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2,946 differentially expressed genes after Knockdown/Knockout SRSF4 protein :

Only display 100 entries as example. Please click "View All" button to see all 2,946 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PKD1	ENSG00000008710.13	1156.57350	559.35110	1.04790	17.83640	15.01170	K562	SRSF4
FAM13A	ENSG00000138640.10	1308.31580	2633.96560	-1.00950	15.63290	11.26400	HepG2	SRSF4
WIZ	ENSG00000011451.13	935.94250	519.76260	0.84850	11.09240	8.85490	K562	SRSF4
PDGFB	ENSG00000100311.12	395.04660	788.57610	-0.99720	12.03110	7.96330	HepG2	SRSF4
ELF3	ENSG00000163435.11	436.58400	853.33780	-0.96690	11.04790	7.15620	HepG2	SRSF4
SPPL2B	ENSG00000005206.12	674.63940	381.39050	0.82270	8.63570	6.64250	K562	SRSF4
MLXIPL	ENSG00000009950.11	476.62960	247.50470	0.94530	8.06460	6.13560	K562	SRSF4
ARHGAP33	ENSG00000004777.14	499.11500	269.68470	0.88800	8.02370	6.10210	K562	SRSF4
MRC2	ENSG00000011028.9	532.61560	293.37510	0.86020	7.99900	6.08170	K562	SRSF4
ZC3H3	ENSG00000014164.6	533.21750	301.92270	0.82040	7.38940	5.54060	K562	SRSF4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

84 RBPs can affect the gene expression of SRSF4 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SRSF4	ENSG00000116350.11	7491.95010	3255.33970	1.20240	52.70030	50.29160	K562	AGGF1
SRSF4	ENSG00000116350.11	9542.41700	3273.12850	1.54360	39.19300	36.87600	K562	RBM15
SRSF4	ENSG00000116350.11	10447.49870	3358.99350	1.63700	22.47110	20.94740	K562	PUS1
SRSF4	ENSG00000116350.11	1513.00180	3588.11600	-1.24580	21.71370	18.55140	K562	SRSF4
SRSF4	ENSG00000116350.11	1621.28390	2518.07510	-0.63520	16.31200	15.05120	HepG2	ETF1
SRSF4	ENSG00000116350.11	7428.44090	3387.37840	1.13280	16.16830	14.35120	K562	AKAP8L
SRSF4	ENSG00000116350.11	5826.22950	3554.26960	0.71290	15.88970	13.50530	K562	ATP5C1
SRSF4	ENSG00000116350.11	602.52230	1240.41790	-1.04170	13.65550	12.10750	HepG2	DDX55
SRSF4	ENSG00000116350.11	1666.55800	2354.56660	-0.49860	12.89040	11.80770	HepG2	PUM1
SRSF4	ENSG00000116350.11	7348.01940	4026.97430	0.86760	14.08120	11.79250	K562	MBNL1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 98 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for SRSF4 is not available on ENCORE database.

21 RBPs can affect the splicing of SRSF4 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	SRSF4	ENSG00000116350.11	chr1	-	29476614	29476704	29474254	29475738	29481207	29481422	-0.27000	0.00000	0.00000	excluded	HepG2	U2AF1
SE	SRSF4	ENSG00000116350.11	chr1	-	29476614	29476704	29474254	29475738	29481207	29481422	-0.32100	0.00000	0.00000	excluded	HepG2	U2AF2
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	0.09200	0.00000	0.00083	included	HepG2	NCBP2
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	0.15500	0.00000	0.00002	included	HepG2	SRSF1
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	0.08300	0.00001	0.00190	included	HepG2	IGF2BP3
SE	SRSF4	ENSG00000116350.11	chr1	-	29495014	29495150	29486886	29487029	29508157	29508402	-0.05700	0.00001	0.00045	excluded	K562	AGGF1
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	-0.05900	0.00002	0.00063	excluded	HepG2	U2AF1
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	0.14500	0.00003	0.01127	included	HepG2	UCHL5
SE	SRSF4	ENSG00000116350.11	chr1	-	29495014	29495150	29486886	29487029	29508157	29508402	0.06300	0.00005	0.00607	included	HepG2	HNRNPL
SE	SRSF4	ENSG00000116350.11	chr1	-	29486375	29486570	29485895	29485998	29486886	29486981	0.09000	0.00008	0.03940	included	HepG2	BCCIP

Showing 1 to 10 of 26 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SRSF4

General Information

Functions

Localization(RBPImage: SRSF4)

Domains (AlphaFold: SRSF4)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for SRSF4 is not avaliable on ENCORE database.

63 proteins are bound to SRSF4 RNA:

eCLIP assay for SRSF4 is not avaliable on ENCORE database.

Diffrential Expression

2,946 differentially expressed genes after Knockdown/Knockout SRSF4 protein :

84 RBPs can affect the gene expression of SRSF4 :

The Knockdown/knockout RNA-Seq assay for SRSF4 is not available on ENCORE database.

21 RBPs can affect the splicing of SRSF4 :

The Knockdown/knockout RNA-Seq assay for SRSF4 is not avaliable on ENCORE database.