UBE2L3

General Information

Gene Name : UBE2L3
Gene ID : ENSG00000185651.14
Essential Gene : Yes

Functions

Novel RBP

Localization(RBPImage: UBE2L3)

Nuclei
Nucleoplasm
Mutually Exclusive from Nucleolus
Cytoplasm
Compartment/Organelle
Mitochondria
Cytosolic

Domains (AlphaFold: UBE2L3)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all UBE2L3 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	UBE2L3	ENSG00000185651.14	98.40	95.99	97.19	ENCSR000AEF
HepG2	UBE2L3	ENSG00000185651.14	40.83	67.47	54.15	ENCSR985KAT
K562	UBE2L3	ENSG00000185651.14	356.34	390.08	373.21	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	UBE2L3-BGKLV25-K562	ENCSR362XMY	UBE2L3	K562	Released		ENCSR620PUP	shRNA	KD% = 69.4,60.0
knockdown followed by RNA-seq	UBE2L3-BGHLV23-HepG2	ENCSR424JSU	UBE2L3	HepG2	Released		ENCSR042QTH	shRNA	KD% = 76.6,85.6

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
UBE2L3	ENCAB492XKE	A300-737A	1			Released, ENCODE4	BGHLV23-57, Released, ENCODE3	BGKLV25-61, Released, ENCODE3
UBE2L3		8721S	1			Submitted, ENCORE2	BGHLV23-57, Released, ENCODE3	BGKLV25-61, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
UBE2L3	K562	shRNA			BGKLV13-5,BGKLV13-6	BGKLV13-1,BGKLV13-2		NotSatisfied	ENCODE3
UBE2L3	K562	shRNA	ENCSR362XMY	ENCSR620PUP	UBE2L3-BGKLV25-61,UBE2L3-BGKLV25-62	NT_BGKLV25-1,NT_BGKLV25-2	69.4,60.0	Released	ENCODE3
UBE2L3	HepG2	shRNA	ENCSR424JSU	ENCSR042QTH	UBE2L3-BGHLV23-57,UBE2L3-BGHLV23-58	non-target-BGHLV23-1,non-target-BGHLV23-2	76.6,85.6	Released	ENCODE3
UBE2L3	K562	CRISPR			BGKcLV17-51,BGKcLV17-52	BGKcLV17-1,BGKcLV17-2		NotSatisfied	ENCODE4
UBE2L3	K562	CRISPR			BGKcLV17-53,BGKcLV17-54	BGKcLV17-1,BGKcLV17-2		NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

UBE2L3: Click to RBP Image Database

Nuclei

Nucleoplasm

Mutually Exclusive from Nucleolus

Cytoplasm

Compartment/Organelle

Mitochondria

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
UBE2L3	Product_ID: A300-737A Lot_ID: 1 Source: Bethyl Labs Target Name: UBE2L3-human
UBE2L3	Product_ID: 8721S Lot_ID: 1 Source: Cell Signaling Technology Target Name: UBE2L3-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for UBE2L3 is not avaliable on ENCORE database.

Proteins bound to RNA

UBE2L3 RNA is bound by 34 proteins.

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eCLIP assay for UBE2L3 is not avaliable on ENCORE database.

34 proteins are bound to UBE2L3 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr22	21976672	21976745	+	3'-UTR	5.13537	72.58334	K562	UPF1
chr22	21976354	21976420	+	3'-UTR	3.82303	72.44811	K562	UPF1
chr22	21965339	21965376	+	intron-Proximal	5.79034	53.91840	K562	AQR
chr22	21976420	21976479	+	3'-UTR	5.47780	52.29945	K562	UPF1
chr22	21976965	21977045	+	3'-UTR	4.52332	47.59714	K562	UPF1
chr22	21976520	21976612	+	3'-UTR	3.38020	45.54717	K562	UPF1
chr22	21976355	21976440	+	3'-UTR	3.55250	43.30456	K562	FAM120A
chr22	21977461	21977550	+	3'-UTR	4.48826	42.87762	K562	UPF1
chr22	21976768	21976807	+	3'-UTR	5.03118	38.23088	K562	UPF1
chr22	21976221	21976294	+	3'-UTR	5.51001	37.84869	K562	IGF2BP1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 145 entries

eCLIP assay for UBE2L3 is not avaliable on ENCORE database.

Diffrential Expression

5,805 differentially expressed genes after Knockdown/Knockout UBE2L3 protein.

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92 RBPs can affect the levels of UBE2L3.

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5,805 differentially expressed genes after Knockdown/Knockout UBE2L3 protein :

Only display 100 entries as example. Please click "View All" button to see all 5,805 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
TSPAN6	ENSG00000000003.10	733.57770	2997.20670	-2.03060	56.35050	53.20240	HepG2	UBE2L3
NR1H4	ENSG00000012504.9	271.39980	988.84190	-1.86530	30.78080	28.15050	HepG2	UBE2L3
MGST1	ENSG00000008394.8	1169.74310	2748.30220	-1.23230	23.32460	20.96310	HepG2	UBE2L3
FUCA2	ENSG00000001036.9	2252.19310	4934.60820	-1.13160	22.57360	20.24430	HepG2	UBE2L3
NCAPD2	ENSG00000010292.8	2736.95760	5930.44750	-1.11560	21.56870	19.29030	HepG2	UBE2L3
IL32	ENSG00000008517.12	5374.03630	2670.82650	1.00860	18.07250	15.93200	HepG2	UBE2L3
MAP3K14	ENSG00000006062.9	732.42370	117.80800	2.63610	17.49960	15.39530	HepG2	UBE2L3
WIZ	ENSG00000011451.13	925.48570	382.80410	1.27350	14.21480	12.28190	HepG2	UBE2L3
SRCIN1	ENSG00000017373.11	640.32230	203.09460	1.65650	13.04570	11.18310	HepG2	UBE2L3
MAPK8IP2	ENSG00000008735.10	226.86370	58.51120	1.95490	12.31260	10.49350	HepG2	UBE2L3
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

92 RBPs can affect the gene expression of UBE2L3 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
UBE2L3	ENSG00000185651.10	196.84840	2670.73420	-3.76210	142.06670	137.98930	HepG2	UBE2L3
UBE2L3	ENSG00000185651.10	1105.65860	2652.11770	-1.26220	63.46260	61.10660	HepG2	UPF1
UBE2L3	ENSG00000185651.10	4012.98010	23482.03340	-2.54880	54.24080	49.81460	K562	UBE2L3
UBE2L3	ENSG00000185651.10	11029.42180	6056.47970	0.86470	38.15470	36.06050	K562	FXR1
UBE2L3	ENSG00000185651.10	718.58230	1995.55800	-1.47360	30.75010	28.41150	HepG2	PABPC4
UBE2L3	ENSG00000185651.10	5897.13980	9853.44860	-0.74060	17.71720	16.28770	K562	HNRNPC
UBE2L3	ENSG00000185651.10	3787.46450	2560.52420	0.56470	17.34690	16.03840	HepG2	UTP3
UBE2L3	ENSG00000185651.10	18883.74500	12808.79640	0.55990	14.90880	13.51780	K562	RBM15
UBE2L3	ENSG00000185651.10	9515.19440	15547.34280	-0.70840	14.48520	12.73050	K562	SF3A3
UBE2L3	ENSG00000185651.10	1425.65960	2279.16910	-0.67690	13.82150	12.57990	HepG2	QKI
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 107 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for UBE2L3 is not available on ENCORE database.

9 RBPs can affect the splicing of UBE2L3 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947200	21921835	21922060	21965145	21965332	-0.43800	0.00000	0.00000	excluded	K562	SF3B4
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947245	21921835	21922060	21965145	21965332	-0.07200	0.00000	0.00000	excluded	HepG2	PRPF8
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947289	21921835	21922060	21965145	21965332	-0.07800	0.00000	0.00000	excluded	K562	AQR
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947226	21921835	21922060	21965145	21965332	-0.18700	0.00000	0.00000	excluded	K562	PUS1
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947245	21921835	21922060	21965145	21965332	-0.07900	0.00000	0.00000	excluded	HepG2	PCBP2
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947200	21921835	21922060	21965145	21965332	0.34600	0.00000	0.00000	included	K562	HNRNPM
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947289	21921835	21922060	21965145	21965332	-0.13700	0.00000	0.00000	excluded	HepG2	SF3A3
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947289	21921835	21922060	21965145	21965332	-0.13600	0.00000	0.00000	excluded	K562	SF3B1
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947200	21921835	21922060	21965145	21965332	-0.27200	0.00037	0.01739	excluded	K562	PRPF8
SE	UBE2L3	ENSG00000185651.10	chr22	+	21947149	21947200	21921835	21922060	21965145	21965332	-0.29200	0.00199	0.06623	excluded	K562	FXR1

Showing 1 to 10 of 11 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

UBE2L3

General Information

Functions

Localization(RBPImage: UBE2L3)

Domains (AlphaFold: UBE2L3)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for UBE2L3 is not avaliable on ENCORE database.

34 proteins are bound to UBE2L3 RNA:

eCLIP assay for UBE2L3 is not avaliable on ENCORE database.

Diffrential Expression

5,805 differentially expressed genes after Knockdown/Knockout UBE2L3 protein :

92 RBPs can affect the gene expression of UBE2L3 :

The Knockdown/knockout RNA-Seq assay for UBE2L3 is not available on ENCORE database.

9 RBPs can affect the splicing of UBE2L3 :

The Knockdown/knockout RNA-Seq assay for UBE2L3 is not avaliable on ENCORE database.