SRSF5

General Information

Gene Name : SRSF5
Gene ID : ENSG00000100650.15
Essential Gene : No

Functions

Splicing regulation
Viral RNA regulation

Localization(RBPImage: SRSF5)

Nuclei
Nucleoplasm
Nucleolus
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: SRSF5)

RRM

Antibody Validation

MBLI: RN082PW

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	SRSF5	ENSG00000100650.15	255.39	251.61	253.50	ENCSR000AEF
HepG2	SRSF5	ENSG00000100650.15	314.14	316.45	315.29	ENCSR985KAT
K562	SRSF5	ENSG00000100650.15	179.86	171.07	175.47	ENCSR000AEO

Assays

7 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 713	ENCSR973HOJ	SRSF5	HepG2	Released	ENCAB069PGU	ENCSR592ESN
eCLIP	eCLIP: 4058	ENCSR124DES	SRSF5	K562	Released	ENCAB038XFS	ENCSR195PZF
knockdown followed by RNA-seq	SRSF5-BGKLV11-K562	ENCSR906RHU	SRSF5	K562	Released		ENCSR129RWD	shRNA	KD% = 23.1,20.5
knockdown followed by RNA-seq	SRSF5-BGHLV14-HepG2	ENCSR447UCG	SRSF5	HepG2	Released		ENCSR491FOC	shRNA	KD% = see BGHLV23,see BGHLV23
knockdown followed by RNA-seq	SRSF5-BGHLV23-HepG2	ENCSR781YNI	SRSF5	HepG2	Released		ENCSR042QTH	shRNA	KD% = 67.0,74.9
knockdown followed by RNA-seq	SRSF5-BGKcLV11-K562	ENCSR528YVC	SRSF5	K562	Released		ENCSR292PXV	CRISPR	KD% = 92.2,96.4
RNA Bind-n-Seq	RBNS: SRSF5	ENCSR914PGB	SRSF5	cell-free sample	Released		ENCSR876NDK

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
SRSF5	713	HepG2	ENCSR973HOJ	ENCSR592ESN	ENCAB069PGU	A303-894A	1	Released	ENCODE4
SRSF5	4058	K562	ENCSR124DES	ENCSR195PZF	ENCAB038XFS	RN082PW	2	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
SRSF5	ENCAB038XFS	RN082PW	1				BGHLV23-45, Released, ENCODE3	BGKcLV11-95, Released, ENCODE4

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
SRSF5	K562	shRNA	ENCSR906RHU	ENCSR129RWD	SRSF5-41-LV11-51,SRSF5-41-LV11-52	Nontarget-LV11-1,nontarget-LV11-2	23.1,20.5	Released	ENCODE3
SRSF5	K562	shRNA			BGKLV25-55,BGKLV25-56	BGKLV25-1,BGKLV25-2		NotSatisfied	ENCODE3
SRSF5	K562	shRNA			BGKLV40-13,BGKLV40-14	BGKLV40-1,BGKLV40-2	0.0,0.0	Others	ENCODE4
SRSF5	HepG2	shRNA	ENCSR447UCG	ENCSR491FOC	SRSF5_41_BGHLV14-55,SRSF5_41_BGHLV14-56	NT_BGHLV14-1,NT_BGHLV14-2	see BGHLV23,see BGHLV23	Released	ENCODE3
SRSF5	HepG2	shRNA	ENCSR781YNI	ENCSR042QTH	SRSF5-BGHLV23-45,SRSF5-BGHLV23-46	non-target-BGHLV23-1,non-target-BGHLV23-2	67.0,74.9	Released	ENCODE3
SRSF5	HepG2	shRNA			BGHLV39-13,BGHLV39-14	BGHLV39-1,BGHLV39-2	0.0,0.0	Others	ENCODE4
SRSF5	K562	CRISPR	ENCSR528YVC	ENCSR292PXV	SRSF5-BGKcLV11-95,SRSF5-BGKcLV11-96	NT-BGKcLV11-1,NT-BGKcLV11-2	92.2,96.4	Released	ENCODE4
SRSF5	K562	CRISPR			BGKcLV11-97,BGKcLV11-98	BGKcLV11-1,BGKcLV11-2	88.6,88.5	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

GeneName	gene_aliases	Status	Project	Note
SRSF5	SRSF5		Released	ENCODE3

Localization Image in HepG2

SRSF5: Click to RBP Image Database

Nuclei

Nucleoplasm

Nucleolus

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
SRSF5	Product_ID: RN082PW Lot_ID: 1 Source: MBLI Target Name: SRSF5-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for SRSF5 is not avaliable on ENCORE database.

The binding motifs of SRSF5

Proteins bound to RNA

SRSF5 RNA is bound by 46 proteins.

CanvasJS.com

eCLIP assay for SRSF5 is not avaliable on ENCORE database.

46 proteins are bound to SRSF5 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr14	70237066	70237110	+	3'-UTR,intron-Distal,intron-Proximal	5.82918	400.00000	HepG2	XPO5
chr14	70238592	70238618	+	3'-UTR,intron-Proximal	5.63262	400.00000	K562	PUM2
chr14	70238620	70238675	+	3'-UTR,intron-Proximal	5.34036	400.00000	K562	PABPC4
chr14	70238618	70238670	+	3'-UTR,intron-Proximal	4.81138	400.00000	K562	PUM2
chr14	70237029	70237066	+	3'-UTR,intron-Distal	5.35867	400.00000	HepG2	XPO5
chr14	70237794	70237822	+	3'-UTR,CDS	4.23540	400.00000	HepG2	BCLAF1
chr14	70235345	70235391	+	CDS	3.98389	400.00000	HepG2	BCLAF1
chr14	70238321	70238367	+	3'-UTR,intron-Distal,intron-Proximal	4.18783	85.72368	K562	PUM2
chr14	70235319	70235391	+	CDS,intron-Proximal	3.29621	80.04814	HepG2	PPIG
chr14	70238616	70238674	+	3'-UTR,intron-Proximal	5.62839	78.53119	K562	DDX6
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 197 entries

eCLIP assay for SRSF5 is not avaliable on ENCORE database.

Diffrential Expression

10,095 differentially expressed genes after Knockdown/Knockout SRSF5 protein.

CanvasJS.com

105 RBPs can affect the levels of SRSF5.

CanvasJS.com

10,095 differentially expressed genes after Knockdown/Knockout SRSF5 protein :

Only display 100 entries as example. Please click "View All" button to see all 10,095 entries.

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PKD1	ENSG00000008710.13	1510.91870	395.55890	1.93340	89.09030	86.34800	HepG2	SRSF5
RHBDD2	ENSG00000005486.12	1467.87730	494.02090	1.57100	61.14490	58.74600	HepG2	SRSF5
CX3CL1	ENSG00000006210.6	3871.68350	1866.63200	1.05240	50.22180	48.03750	HepG2	SRSF5
TNFRSF12A	ENSG00000006327.9	1576.11200	650.82040	1.27590	45.07760	42.99070	HepG2	SRSF5
ITGA3	ENSG00000005884.13	1165.40870	121.98160	3.25600	39.19140	37.21390	HepG2	SRSF5
CRLF1	ENSG00000006016.6	7897.50760	4563.90030	0.79100	37.05510	35.13030	HepG2	SRSF5
PLXND1	ENSG00000004399.8	4822.52830	2168.52670	1.15300	34.67480	32.81020	HepG2	SRSF5
MGST1	ENSG00000008394.8	1391.81280	2624.19650	-0.91490	33.40420	31.56740	HepG2	SRSF5
DBNDD1	ENSG00000003249.9	1621.90650	779.24630	1.05740	33.17600	31.34780	HepG2	SRSF5
BAIAP3	ENSG00000007516.9	404.46730	121.12020	1.73950	27.76300	26.06050	HepG2	SRSF5
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

105 RBPs can affect the gene expression of SRSF5 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SRSF5	ENSG00000100650.11	2274.75900	7335.75910	-1.68920	144.35900	140.92750	HepG2	SRSF5
SRSF5	ENSG00000100650.11	4487.74710	7306.17690	-0.70310	32.38690	31.15960	HepG2	SRSF1
SRSF5	ENSG00000100650.11	4049.73700	7122.92500	-0.81460	30.96880	29.60310	HepG2	RBM22
SRSF5	ENSG00000100650.11	3107.99740	5050.25790	-0.70040	29.87880	28.38800	HepG2	ACO1
SRSF5	ENSG00000100650.11	5441.36930	8342.80150	-0.61660	29.61940	27.94670	HepG2	SUCLG1
SRSF5	ENSG00000100650.11	5519.07570	7886.97450	-0.51500	24.32030	22.91890	HepG2	PUM1
SRSF5	ENSG00000100650.11	10032.90750	5217.04930	0.94330	19.50560	18.09330	K562	PUS1
SRSF5	ENSG00000100650.11	4477.40720	6723.39020	-0.58650	18.63940	17.45870	HepG2	PTBP1
SRSF5	ENSG00000100650.11	4848.93230	7299.82090	-0.59020	17.77500	16.42760	HepG2	CELF1
SRSF5	ENSG00000100650.11	6491.39240	3570.39960	0.86230	18.06730	16.35290	HepG2	NSUN2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 124 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for SRSF5 is not available on ENCORE database.

72 RBPs can affect the splicing of SRSF5 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	SRSF5	ENSG00000100650.11	chr14	+	70236738	70236759	70235898	70235968	70237183	70237257	0.06600	0.00000	included	K562	AQR
SE	SRSF5	ENSG00000100650.11	chr14	+	70235737	70235968	70235475	70235613	70237183	70237257	-0.09000	0.00000	excluded	K562	AQR
SE	SRSF5	ENSG00000100650.11	chr14	+	70235898	70235968	70235514	70235613	70237183	70237257	-0.08500	0.00000	excluded	K562	PUS1
A3SS	SRSF5	ENSG00000100650.11	chr14	+	70235299	70235391	70235329	70235391	70234854	70234999	0.79800	0.00000	longer	K562	AQR
A3SS	SRSF5	ENSG00000100650.11	chr14	+	70236227	70237257	70237183	70237257	70235898	70235968	0.13400	0.00000	longer	K562	AQR
A3SS	SRSF5	ENSG00000100650.11	chr14	+	70235898	70237257	70237183	70237257	70235514	70235613	-0.15000	0.00000	shorter	K562	PUS1
A3SS	SRSF5	ENSG00000100650.11	chr14	+	70235898	70237257	70237183	70237257	70235514	70235613	-0.06000	0.00120	shorter	K562	UTP18
A3SS	SRSF5	ENSG00000100650.11	chr14	+	70235898	70237257	70237183	70237257	70235514	70235613	-0.07100	0.00000	shorter	HepG2	U2AF1
A5SS	SRSF5	ENSG00000100650.11	chr14	+	70233809	70234097	70233809	70233972	70234854	70234999	0.07000	0.00000	longer	K562	PUS1
A5SS	SRSF5	ENSG00000100650.11	chr14	+	70233809	70234097	70233809	70233972	70234854	70234999	0.16500	0.00000	longer	K562	AQR

Showing 1 to 10 of 176 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

SRSF5

General Information

Functions

Localization(RBPImage: SRSF5)

Domains (AlphaFold: SRSF5)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for SRSF5 is not avaliable on ENCORE database.

46 proteins are bound to SRSF5 RNA:

eCLIP assay for SRSF5 is not avaliable on ENCORE database.

Diffrential Expression

10,095 differentially expressed genes after Knockdown/Knockout SRSF5 protein :

105 RBPs can affect the gene expression of SRSF5 :

The Knockdown/knockout RNA-Seq assay for SRSF5 is not available on ENCORE database.

72 RBPs can affect the splicing of SRSF5 :

The Knockdown/knockout RNA-Seq assay for SRSF5 is not avaliable on ENCORE database.