WDR43

General Information

Gene Name : WDR43
Gene ID : ENSG00000163811.11
Essential Gene : Yes

Functions

rRNA processing

Localization(RBPImage: WDR43)

Nuclei
Nucleolus
Cytoplasm
Mitotic-Apparatus (Spindle; Centrosomes)

Domains (AlphaFold: WDR43)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A302-478A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	WDR43	ENSG00000163811.11	65.29	57.43	61.36	ENCSR000AEF
HepG2	WDR43	ENSG00000163811.11	115.21	116.97	116.09	ENCSR985KAT
K562	WDR43	ENSG00000163811.11	56.30	55.89	56.09	ENCSR000AEO

Assays

4 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 517	ENCSR349CMI	WDR43	K562	Released	ENCAB350LKS	ENCSR404ABA
eCLIP	eCLIP: 602	ENCSR085JPB	WDR43	HepG2	Released	ENCAB350LKS	ENCSR722KGI
knockdown followed by RNA-seq	WDR43-BGKLV34-K562	ENCSR341PZW	WDR43	K562	Released		ENCSR174OYC	shRNA	KD% = 85.9,89.0
knockdown followed by RNA-seq	WDR43-BGHcLV12-HepG2	ENCSR606QVN	WDR43	HepG2	Released		ENCSR827PII	CRISPR	KD% = 71.9,68.1

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
WDR43	517	K562	ENCSR349CMI	ENCSR404ABA	ENCAB350LKS	A302-478A	1	Released	ENCODE4
WDR43	602	HepG2	ENCSR085JPB	ENCSR722KGI	ENCAB350LKS	A302-478A	1	Released	ENCODE4

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
WDR43	ENCAB350LKS	A302-478A	1		Released, ENCODE3	Released, ENCODE3	BGHcLV12-87, Released, ENCODE4	BGKLV34-51, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
WDR43	K562	shRNA	ENCSR341PZW	ENCSR174OYC	WDR43_BGKLV34-51,WDR43_BGKLV34-52	NT_BGKLV34-1,NT_BGKLV34-2	85.9,89.0	Released	ENCODE3
WDR43	HepG2	shRNA			BGHLV22-55,BGHLV22-56	BGHLV22-1,BGHLV22-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
WDR43	HepG2	shRNA			BGHLV27-59,BGHLV27-60	BGHLV27-1,BGHLV27-2		NotSatisfied	ENCODE3
WDR43	HepG2	shRNA			BGHLV33-39,BGHLV33-40	BGHLV33-1,BGHLV33-2	0.0,0.0	NotSatisfied	ENCODE3
WDR43	HepG2	shRNA			BGHLV37-81,BGHLV37-82	BGHLV37-1,BGHLV37-2	NO clear band,	NotSatisfied	ENCODE3
WDR43	HepG2	shRNA			BGHLV37-83,BGHLV37-84	BGHLV37-1,BGHLV37-2	NO clear band,	NotSatisfied	ENCODE3
WDR43	HepG2	shRNA			BGHLV37-85,BGHLV37-86	BGHLV37-1,BGHLV37-2	NO clear band,	NotSatisfied	ENCODE3
WDR43	K562	CRISPR			BGKcLV06-41,BGKcLV06-42	BGKcLV06-1,BGKcLV06-2		NotSatisfied	ENCODE3
WDR43	HepG2	CRISPR			BGHcLV07-41,BGHcLV07-42	BGHcLV07-1,BGHcLV07-2	0.0,0.0	NotSatisfied	ENCODE3
WDR43	HepG2	CRISPR			BGHcLV09-27,BGHcLV09-28	BGHcLV09-1,BGHcLV09-2	0.0,0.0	NotSatisfied	ENCODE4
WDR43	HepG2	CRISPR			BGHcLV09-29,BGHcLV09-30	BGHcLV09-1,BGHcLV09-2	0.0,0.0	NotSatisfied	ENCODE4
WDR43	HepG2	CRISPR			BGHcLV12-85,BGHcLV12-86	BGHcLV12-1,BGHcLV12-2	57.1,58.9	NotSatisfied	ENCODE4
WDR43	HepG2	CRISPR	ENCSR606QVN	ENCSR827PII	WDR43-BGHcLV12-87,WDR43-BGHcLV12-88	NT-BGHcLV12-1,NT-BGHcLV12-2	71.9,68.1	Released	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

WDR43: Click to RBP Image Database

Nuclei

Nucleolus

Cytoplasm

Mitotic-Apparatus (Spindle; Centrosomes)

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
WDR43	Product_ID: A302-478A Lot_ID: 1 Source: Bethyl Labs Target Name: WDR43-human

RNA Targets

WDR43 protein binds to 302 RNA regions.

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Proteins bound to RNA

WDR43 RNA is bound by 52 proteins.

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WDR43 protein has 302 RNA targets:

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
WDR43	chr11	93454913	93454957	+	4.39907	400.00000	K562	KIAA1731	intron-Distal
WDR43	chr9	136216310	136216327	+	3.18156	400.00000	K562	RPL7A	intron-Proximal
WDR43	chr11	93454831	93454903	+	4.63793	83.38314	K562	KIAA1731	intron-Distal
WDR43	chr11	122929615	122929619	-	3.69543	71.98649	K562	HSPA8	5'-UTR,intron-Distal,intron-Proximal
WDR43	chr19	13054602	13054736	+	3.34438	58.59441	K562	CALR	3'-UTR,5'-UTR,CDS,intron-Proximal
WDR43	chr9	100777650	100777762	+	3.46880	56.20946	K562	ANP32B	3'-UTR,CDS
WDR43	chr2	203141171	203141234	+	4.60396	52.57662	K562	NOP58	intron-Distal
WDR43	chr20	26189971	26189989	-	5.91925	30.72231	HepG2	MIR663A	intron-Distal,intron-Proximal
WDR43	chr9	100777645	100777650	+	3.81984	26.51266	K562	ANP32B	CDS,intron-Proximal
WDR43	chr11	76594456	76594464	+	5.78905	23.81672	HepG2	ACER3	intron-Distal

Showing 1 to 10 of 100 entries

52 proteins are bound to WDR43 RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr2	29136569	29136590	+	intron-Distal	5.30552	400.00000	HepG2	NOLC1
chr2	29150902	29150928	+	intron-Distal	5.16633	400.00000	HepG2	NOLC1
chr2	29136571	29136615	+	intron-Distal	3.18686	400.00000	HepG2	DKC1
chr2	29136555	29136569	+	intron-Distal	4.85077	400.00000	HepG2	NOLC1
chr2	29136571	29136584	+	intron-Distal	4.23655	400.00000	K562	AATF
chr2	29150847	29150902	+	intron-Distal	4.57718	400.00000	HepG2	NOLC1
chr2	29136553	29136571	+	intron-Distal	3.39214	400.00000	HepG2	DKC1
chr2	29136584	29136616	+	intron-Distal	4.75964	400.00000	K562	AATF
chr2	29149957	29149970	+	intron-Distal	4.24731	400.00000	HepG2	NOLC1
chr2	29136555	29136578	+	intron-Distal	4.13412	400.00000	K562	SMNDC1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 213 entries

No autoregularoty interactions between protein WDR43 and its RNA.

Diffrential Expression

4,552 differentially expressed genes after Knockdown/Knockout WDR43 protein.

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109 RBPs can affect the levels of WDR43.

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4,552 differentially expressed genes after Knockdown/Knockout WDR43 protein :

Only display 100 entries as example. Please click "View All" button to see all 4,552 entries.

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SLC4A1	ENSG00000004939.9	1441.19520	344.04270	2.06650	62.88300	60.16270	K562	WDR43
ITGA2B	ENSG00000005961.13	193.34450	613.39900	-1.66570	38.84480	36.38690	K562	WDR43
ACSM3	ENSG00000005187.7	1272.72760	2223.08820	-0.80460	27.92650	25.71020	K562	WDR43
CYP51A1	ENSG00000001630.11	3408.21430	5553.91840	-0.70450	27.04420	24.84490	K562	WDR43
MGST1	ENSG00000008394.8	295.62000	693.68640	-1.23050	26.56860	24.38300	K562	WDR43
SLC25A5	ENSG00000005022.5	5803.37350	8610.95490	-0.56930	23.60020	21.50010	K562	WDR43
QPCTL	ENSG00000011478.7	148.03360	411.44040	-1.47480	23.02990	20.94920	K562	WDR43
CREBBP	ENSG00000005339.8	3455.06990	1993.49740	0.79330	20.12940	18.16680	K562	WDR43
ETV1	ENSG00000006468.9	205.89230	477.02810	-1.21220	19.14750	17.21140	K562	WDR43
WAS	ENSG00000015285.6	99.49210	332.72240	-1.74170	17.77430	15.90050	K562	WDR43
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

109 RBPs can affect the gene expression of WDR43 :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
WDR43	ENSG00000163811.7	1202.50320	2762.93410	-1.20020	63.19000	60.46030	K562	WDR43
WDR43	ENSG00000163811.7	6859.75770	3729.41650	0.87910	40.71440	38.50210	K562	DDX47
WDR43	ENSG00000163811.7	3053.96770	1628.36270	0.90720	30.26330	28.40050	K562	FXR1
WDR43	ENSG00000163811.7	2611.57640	4217.59580	-0.69150	27.06180	25.91430	HepG2	SRFBP1
WDR43	ENSG00000163811.7	2421.99620	3891.26190	-0.68400	22.14040	20.53990	HepG2	AUH
WDR43	ENSG00000163811.7	1634.90390	3444.40930	-1.07510	21.73560	20.23220	HepG2	HNRNPLL
WDR43	ENSG00000163811.7	2345.02690	3661.87610	-0.64300	21.19490	20.06660	HepG2	SSB
WDR43	ENSG00000163811.7	861.52810	1892.50880	-1.13530	18.31990	16.86670	K562	HNRNPC
WDR43	ENSG00000163811.7	2068.38730	3105.22020	-0.58620	17.17290	15.54910	K562	UPF1
WDR43	ENSG00000163811.7	3324.11110	1973.38370	0.75220	17.33770	15.31880	K562	SYNCRIP
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 136 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

264 alternative splicing events after Knockdown/Knockout WDR43 protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	IVNS1ABP	ENSG00000116679.11	chr1	-	185275616	185275757	185274667	185274775	185276145	185276271	0.11200	0.00008	included	K562	WDR43
SE	MBNL1	ENSG00000152601.13	chr3	+	152164492	152164546	152163070	152163328	152165408	152165562	0.22600	0.00000	included	K562	WDR43
SE	TRA2A	ENSG00000164548.6	chr7	-	23561739	23562051	23561325	23561459	23571407	23571599	0.23400	0.00054	included	K562	WDR43
SE	SREK1	ENSG00000153914.11	chr5	+	65454636	65454760	65449290	65449424	65455046	65455162	0.16100	0.00003	included	K562	WDR43
SE	PTBP1	ENSG00000011304.12	chr19	+	805491	805569	805012	805187	806407	806556	0.13600	0.00002	included	K562	WDR43
SE	FLOT2	ENSG00000132589.11	chr17	-	27212874	27212965	27211242	27211333	27215962	27216044	0.06300	0.00012	included	K562	WDR43
SE	SNHG16	ENSG00000163597.10	chr17	+	74555026	74555125	74553873	74553939	74557369	74557480	0.16400	0.00082	included	K562	WDR43
SE	BAG1	ENSG00000107262.12	chr9	-	33262102	33262244	33261084	33261167	33262699	33262828	0.21000	0.00032	included	K562	WDR43
SE	FGFR1OP	ENSG00000213066.7	chr6	+	167426995	167427055	167424300	167424381	167435896	167436123	0.23100	0.00189	included	K562	WDR43
SE	SLC25A51	ENSG00000122696.8	chr9	-	37903527	37903554	37899825	37899947	37904064	37904130	0.24600	0.00344	included	K562	WDR43

Showing 1 to 10 of 100 entries

4 RBPs can affect the splicing of WDR43 :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	WDR43	ENSG00000163811.7	chr2	+	29140758	29140861			29136944	29137074	29145784	29145849	-0.07200	0.00000	0.00001	excluded	K562	AQR
A3SS	WDR43	ENSG00000163811.7	chr2	+	29159754	29159819			29159787	29159819	29158270	29158473	0.05300	0.00000	0.00001	longer	K562	AQR
A3SS	WDR43	ENSG00000163811.7	chr2	+	29165154	29165247			29165177	29165247	29164326	29164440	0.27700	0.00000	0.00000	longer	K562	AQR
RI	WDR43	ENSG00000163811.7	chr2	+	29169291	29169535			29169291	29169365	29169465	29169535	0.08600	0.00000	0.00000	included	K562	AQR
MXE	WDR43	ENSG00000163811.7	chr2	+	29136944	29137074	29140758	29140861	29135455	29135576	29145784	29145849	0.08000	0.00000	0.00002	1included	K562	AQR
MXE	WDR43	ENSG00000163811.7	chr2	+	29159754	29159819	29164300	29164440	29158270	29158473	29165177	29165247	0.06500	0.00000	0.00000	1included	K562	AQR
SE	WDR43	ENSG00000163811.7	chr2	+	29135375	29135576			29129325	29129447	29136934	29137025	-0.05400	0.00003	0.00068	excluded	K562	AQR
SE	WDR43	ENSG00000163811.7	chr2	+	29164326	29164440			29152403	29152576	29165177	29165247	-0.06400	0.00010	0.00164	excluded	K562	AQR
SE	WDR43	ENSG00000163811.7	chr2	+	29140758	29140861			29136944	29137074	29145784	29145849	-0.10300	0.00188	0.03317	excluded	K562	U2AF2
MXE	WDR43	ENSG00000163811.7	chr2	+	29152444	29152576	29164326	29164440	29150434	29150566	29165177	29165247	0.09200	0.00243	0.01959	1included	K562	AQR

Showing 1 to 10 of 12 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

WDR43

General Information

Functions

Localization(RBPImage: WDR43)

Domains (AlphaFold: WDR43)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

WDR43 protein has 302 RNA targets:

52 proteins are bound to WDR43 RNA:

No autoregularoty interactions between protein WDR43 and its RNA.

Diffrential Expression

4,552 differentially expressed genes after Knockdown/Knockout WDR43 protein :

109 RBPs can affect the gene expression of WDR43 :

264 alternative splicing events after Knockdown/Knockout WDR43 protein :

4 RBPs can affect the splicing of WDR43 :

No autoregularoty alternative splicing events between protein WDR43 and its RNA.