NKRF

General Information

Gene Name : NKRF
Gene ID : ENSG00000186416.14
Essential Gene : No

Functions

Novel RBP

Localization(RBPImage: NKRF)

Nuclei
Nucleoplasm
Nucleolus
Cytoplasm
Cytosolic
Nuclear release mitosis

Domains (AlphaFold: NKRF)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

View validation images for all NKRF antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	NKRF	ENSG00000186416.14	15.97	13.81	14.89	ENCSR000AEF
HepG2	NKRF	ENSG00000186416.14	4.94	7.83	6.38	ENCSR985KAT
K562	NKRF	ENSG00000186416.14	20.71	21.47	21.09	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
eCLIP	eCLIP: 546	ENCSR277DEO	NKRF	HepG2	Released	ENCAB893CIV	ENCSR479XOI
knockdown followed by RNA-seq	NKRF-BGKLV36-K562	ENCSR231PWH	NKRF	K562	Released		ENCSR619PCR	shRNA	KD% = 52.6,54.5
knockdown followed by RNA-seq	NKRF-BGHLV27-HepG2	ENCSR517JDK	NKRF	HepG2	Released		ENCSR246RRQ	shRNA	KD% = 54.6,48.9

eCLIP

RBP	Exp_UID	Cell_Line	ENCODE_ACCESSION	Control_ENCODE_ACCESSION	ENCODE_Antibody_ID	Antibody	Lot_num	Status	Project
NKRF	546	HepG2	ENCSR277DEO	ENCSR479XOI	ENCAB893CIV	A304-016A	1	Released	ENCODE3

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
NKRF	ENCAB893CIV	A304-016A	1		Released, ENCODE3		BGHLV27-37, Released, ENCODE3	BGKLV36-39, Released, ENCODE3
NKRF		63409S	1		Released, ENCODE3	Submitted, ENCORE2	BGHLV27-37, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
NKRF	K562	shRNA			BGKLV32-45,BGKLV32-46	BGKLV32-1,BGKLV32-2	42.9,46.0	NotSatisfied	ENCODE3
NKRF	K562	shRNA			BGKLV36-37,BGKLV36-38	BGKLV36-1,BGKLV36-2		NotSatisfied	ENCODE3
NKRF	K562	shRNA	ENCSR231PWH	ENCSR619PCR	NKRF-BGKLV36-39,NKRF-BGKLV36-40	NT-BGKLV36-1C,NT-BGKLV36-2C	52.6,54.5	Released	ENCODE4
NKRF	HepG2	shRNA			BGHLV18-31,BGHLV18-32	BGHLV18-1,BGHLV18-2		NotSatisfied	ENCODE3
NKRF	HepG2	shRNA	ENCSR517JDK	ENCSR246RRQ	NKRF_BGHLV27-37,NKRF_BGHLV27-38	non-target_BGHLV27-1,non-target_BGHLV27-2	54.6,48.9	Released	ENCODE3

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

NKRF: Click to RBP Image Database

Nuclei

Nucleoplasm

Nucleolus

Cytoplasm

Cytosolic

Nuclear release mitosis

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
NKRF	Product_ID: A304-016A Lot_ID: 1 Source: Bethyl Labs Target Name: NKRF-human
NKRF	Product_ID: 63409S Lot_ID: 1 Source: Cell Signaling Technology Target Name: NKRF-human

RNA Targets

NKRF protein binds to 3,783 RNA regions.

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Proteins bound to RNA

NKRF RNA is bound by 7 proteins.

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NKRF protein has 3,783 RNA targets:

Only display 100 entries as example. Please click "View All" button to see all 3,783 entries.

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RBP	Chr	Start	End	Strand	log2(fold change)	-log(p-value)	Cell Line	Matched Gene	Matched Area
NKRF	chr17	80192284	80192358	+	3.63329	35.37664	HepG2	SLC16A3	5'-UTR,intron-Distal
NKRF	chr11	706834	706969	+	3.70773	33.49254	HepG2	EPS8L2	intron-Distal,intron-Proximal
NKRF	chr2	231903285	231903369	+	3.86587	30.28899	HepG2	C2orf72	intron-Distal
NKRF	chr22	29280153	29280272	+	3.38286	27.59673	HepG2	ZNRF3	intron-Distal,intron-Proximal
NKRF	chr19	4066091	4066145	-	5.79698	25.82946	HepG2	ZBTB7A	intron-Distal
NKRF	chr7	139875778	139875871	-	4.22256	21.95164	HepG2	JHDM1D	intron-Distal
NKRF	chr8	142412148	142412237	+	3.31459	17.64438	HepG2	PTP4A3	intron-Distal
NKRF	chr4	1238161	1238288	-	3.19827	16.90770	HepG2	CTBP1	intron-Distal
NKRF	chr5	132166093	132166266	-	3.28022	16.86541	HepG2	SHROOM1	intron-Distal
NKRF	chr1	228330371	228330418	+	4.17518	15.49444	HepG2	GUK1	intron-Distal

Showing 1 to 10 of 100 entries

7 proteins are bound to NKRF RNA:

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Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chrX	118739415	118739646	-	intron-Distal	4.18721	9.46556	HepG2	PRPF4
chrX	118739422	118739591	-	intron-Distal	4.20548	7.48569	K562	GTF2F1
chrX	118723635	118723687	-	CDS,intron-Distal	4.72954	5.86954	K562	UCHL5
chrX	118722351	118722405	-	3'-UTR,intron-Distal	4.74031	5.09993	K562	PUM2
chrX	118739407	118739516	-	intron-Distal	3.34329	4.35027	K562	EFTUD2
chrX	118739658	118739726	-	5'-UTR,intron-Distal	4.03128	4.16174	HepG2	PRPF8
chrX	118739482	118739623	-	intron-Distal	4.84547	3.99130	HepG2	FAM120A
chrX	118724891	118724990	-	CDS,intron-Distal	3.24132	3.12941	K562	UCHL5
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 8 of 8 entries

No autoregularoty interactions between protein NKRF and its RNA.

Diffrential Expression

905 differentially expressed genes after Knockdown/Knockout NKRF protein.

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59 RBPs can affect the levels of NKRF.

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905 differentially expressed genes after Knockdown/Knockout NKRF protein :

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Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
SLC12A3	ENSG00000070915.5	91.04110	340.80850	-1.90440	17.89510	14.60090	HepG2	NKRF
CYP24A1	ENSG00000019186.5	422.73220	132.31730	1.67560	16.10450	13.07140	HepG2	NKRF
PRSS8	ENSG00000052344.11	1224.61940	501.20250	1.28880	10.13410	7.48490	HepG2	NKRF
PLEKHB1	ENSG00000021300.9	867.64920	1664.35130	-0.93980	9.77690	7.18880	HepG2	NKRF
TNS1	ENSG00000079308.12	7570.03180	14383.89940	-0.92610	9.14590	6.64760	HepG2	NKRF
WWTR1	ENSG00000018408.10	2227.25390	4378.68000	-0.97520	8.73220	6.27780	HepG2	NKRF
DPYSL2	ENSG00000092964.12	2683.05510	4701.00940	-0.80910	8.15510	5.75480	HepG2	NKRF
FAM107B	ENSG00000065809.9	1408.84700	713.12100	0.98220	7.77870	5.44660	HepG2	NKRF
PRR11	ENSG00000068489.8	558.65870	1029.79290	-0.88230	7.72510	5.40090	HepG2	NKRF
WNK1	ENSG00000060237.12	7943.11750	13358.99080	-0.75000	7.37500	5.09500	HepG2	NKRF
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

59 RBPs can affect the gene expression of NKRF :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
NKRF	ENSG00000186416.8	885.58640	1486.80310	-0.74750	18.18300	16.51960	K562	UPF1
NKRF	ENSG00000186416.8	462.32370	874.47400	-0.91950	12.72470	11.52110	K562	HNRNPC
NKRF	ENSG00000186416.8	977.61260	1402.21650	-0.52040	10.95660	9.60780	K562	NSUN2
NKRF	ENSG00000186416.8	164.69190	389.09040	-1.24030	10.48140	9.25840	HepG2	EIF4A3
NKRF	ENSG00000186416.8	598.86550	305.74000	0.96980	9.54540	8.36440	HepG2	U2AF1
NKRF	ENSG00000186416.8	706.91380	1116.36570	-0.65920	7.78450	6.44810	K562	GPKOW
NKRF	ENSG00000186416.8	889.09330	1274.20610	-0.51920	7.49650	6.39780	K562	EIF2S2
NKRF	ENSG00000186416.8	771.87810	1169.27550	-0.59920	7.62690	6.31830	K562	CPSF6
NKRF	ENSG00000186416.8	743.80720	1189.72130	-0.67760	7.78570	6.30660	K562	FMR1
NKRF	ENSG00000186416.8	1239.83960	875.61900	0.50170	7.06840	6.16210	K562	FXR1
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 68 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

110 alternative splicing events after Knockdown/Knockout NKRF protein :

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Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	NUPL1	ENSG00000139496.11	chr13	+	25883384	25883420	25881943	25882086	25887020	25887170	0.19600	0.00014	included	HepG2	NKRF
SE	SPTAN1	ENSG00000197694.9	chr9	+	131390203	131390221	131389664	131389777	131392599	131392654	0.05500	0.00265	included	HepG2	NKRF
SE	MORF4L2	ENSG00000123562.12	chrX	-	102939608	102939657	102933426	102933579	102940098	102940188	0.23000	0.00000	included	HepG2	NKRF
SE	TCERG1	ENSG00000113649.7	chr5	+	145841580	145841659	145838777	145838900	145843113	145843356	0.10700	0.00027	included	HepG2	NKRF
SE	HNRNPD	ENSG00000138668.14	chr4	-	83292680	83292737	83280696	83280792	83294598	83295109	0.15700	0.00091	included	HepG2	NKRF
SE	SH3D19	ENSG00000109686.12	chr4	-	152065371	152065440	152065061	152065202	152069073	152069338	0.14900	0.00586	included	HepG2	NKRF
SE	RALY	ENSG00000125970.7	chr20	+	32661624	32661672	32661368	32661441	32663679	32663845	0.08100	0.00439	included	HepG2	NKRF
SE	SLC35D2	ENSG00000130958.7	chr9	-	99113384	99113453	99107580	99107683	99122435	99122503	0.22000	0.00000	included	HepG2	NKRF
SE	ACSL3	ENSG00000123983.9	chr2	+	223765391	223765498	223752547	223752606	223773450	223773754	0.11900	0.00633	included	HepG2	NKRF
SE	PEG3	ENSG00000198300.8	chr19	-	57337755	57337831	57335653	57335795	57347385	57347472	0.15300	0.00439	included	HepG2	NKRF

Showing 1 to 10 of 100 entries

NKRF

General Information

Functions

Localization(RBPImage: NKRF)

Domains (AlphaFold: NKRF)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

NKRF protein has 3,783 RNA targets:

7 proteins are bound to NKRF RNA:

No autoregularoty interactions between protein NKRF and its RNA.

Diffrential Expression

905 differentially expressed genes after Knockdown/Knockout NKRF protein :

59 RBPs can affect the gene expression of NKRF :

110 alternative splicing events after Knockdown/Knockout NKRF protein :

No RBPs affect the alternative splcing events of NKRF on ENCORE database.

No autoregularoty alternative splicing events between protein NKRF and its RNA.