MAK16

General Information

Gene Name : MAK16
Gene ID : ENSG00000198042.10
Essential Gene : Yes

Functions

Novel RBP

Localization(RBPImage: MAK16)

Nuclei
Nucleolus

Domains (AlphaFold: MAK16)

No checked domain (RRM, ZNF, KH, Helicase, Nuclease, dRBM or PUM_HD)

Antibody Validation

Bethyl Labs: A301-232A

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	MAK16	ENSG00000198042.10	35.97	28.26	32.12	ENCSR000AEF
HepG2	MAK16	ENSG00000198042.10	17.18	21.31	19.24	ENCSR985KAT
K562	MAK16	ENSG00000198042.10	42.16	49.08	45.62	ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	MAK16-BGKLV32-K562	ENCSR517JHY	MAK16	K562	Released		ENCSR143COQ	shRNA	KD% = 58.8,56.0
knockdown followed by RNA-seq	MAK16-BGHcLV12-HepG2	ENCSR624YDI	MAK16	HepG2	Released		ENCSR827PII	CRISPR	KD% = 97.5,96.2

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	Phosphate	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
MAK16	ENCAB777CMB	A301-232A	1				BGHcLV12-43, Released, ENCODE4	BGKLV32-37, Released, ENCODE3

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
MAK16	K562	shRNA	ENCSR517JHY	ENCSR143COQ	MAK16_BGKLV32-37,MAK16_BGKLV32-38	NT_BGKLV32-1,NT_BGKLV32-2	58.8,56.0	Released	ENCODE3
MAK16	K562	shRNA			BGKLV34-19,BGKLV34-20	BGKLV34-1,BGKLV34-2		NotSatisfied	ENCODE3
MAK16	HepG2	shRNA			BGHLV18-25,BGHLV18-26	BGHLV18-1,BGHLV18-2		NotSatisfied	ENCODE3
MAK16	HepG2	shRNA			BGHLV20-43,BGHLV20-44	BGHLV20-1,BGHLV20-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
MAK16	HepG2	shRNA			BGHLV27-29,BGHLV27-30	BGHLV27-1,BGHLV27-2		NotSatisfied	ENCODE3
MAK16	HepG2	shRNA			BGHLV33-15,BGHLV33-16	BGHLV33-1,BGHLV33-2		NotSatisfied	ENCODE3
MAK16	HepG2	CRISPR	ENCSR624YDI	ENCSR827PII	MAK16-BGHcLV12-43,MAK16-BGHcLV12-44	NT-BGHcLV12-1,NT-BGHcLV12-2	97.5,96.2	Released	ENCODE4
MAK16	HepG2	CRISPR			BGHcLV12-45,BGHcLV12-46	BGHcLV12-1,BGHcLV12-2	96.9,97.4	NotSatisfied	ENCODE4

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

MAK16: Click to RBP Image Database

Nuclei

Nucleolus

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
MAK16	Product_ID: A301-232A Lot_ID: 1 Source: Bethyl Labs Target Name: MAK16-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for MAK16 is not avaliable on ENCORE database.

Proteins bound to RNA

MAK16 RNA is bound by 6 proteins.

CanvasJS.com

eCLIP assay for MAK16 is not avaliable on ENCORE database.

6 proteins are bound to MAK16 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr8	33356393	33356460	+	3'-UTR	4.56322	16.75955	K562	PUM2
chr8	33357579	33357653	+	3'-UTR	3.65710	14.17328	K562	FAM120A
chr8	33355949	33356014	+	3'-UTR,CDS,intron-Proximal	3.63280	9.11524	K562	UCHL5
chr8	33358340	33358425	+	3'-UTR	4.55398	4.96452	K562	YBX3
chr8	33346067	33346148	+	CDS,intron-Proximal	3.03211	4.35865	K562	YBX3
chr8	33356969	33357021	+	3'-UTR	3.52968	4.01928	K562	FAM120A
chr8	33355940	33356047	+	3'-UTR,CDS,intron-Proximal	3.67666	3.87469	HepG2	BCLAF1
chr8	33356283	33356336	+	3'-UTR	3.63414	3.66308	K562	UPF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 8 of 8 entries

eCLIP assay for MAK16 is not avaliable on ENCORE database.

Diffrential Expression

2,914 differentially expressed genes after Knockdown/Knockout MAK16 protein.

CanvasJS.com

110 RBPs can affect the levels of MAK16.

CanvasJS.com

2,914 differentially expressed genes after Knockdown/Knockout MAK16 protein :

Only display 100 entries as example. Please click "View All" button to see all 2,914 entries.

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
VIM	ENSG00000026025.9	16490.99100	9405.48680	0.81000	20.48680	16.93480	K562	MAK16
TENM1	ENSG00000009694.9	1144.83550	2201.69960	-0.94350	19.11680	15.71970	K562	MAK16
TMSB10	ENSG00000034510.4	9220.86240	5290.95160	0.80130	16.77950	13.49630	K562	MAK16
BOD1L1	ENSG00000038219.8	1498.69280	2711.54110	-0.85540	15.88930	12.69630	K562	MAK16
AKAP11	ENSG00000023516.7	1465.65350	2550.31280	-0.79910	10.50710	7.96740	K562	MAK16
GOPC	ENSG00000047932.9	994.90920	1702.52450	-0.77500	9.51620	7.14030	K562	MAK16
REV3L	ENSG00000009413.11	1086.18850	1684.66460	-0.63320	8.49150	6.21170	K562	MAK16
DNAJC11	ENSG00000007923.11	1417.10970	2223.36010	-0.64980	8.10300	5.87030	K562	MAK16
ACSM3	ENSG00000005187.7	7917.39260	5789.22740	0.45160	6.55880	4.61200	K562	MAK16
GRN	ENSG00000030582.12	2492.08680	1697.41540	0.55390	6.33600	4.44300	K562	MAK16
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

110 RBPs can affect the gene expression of MAK16 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
MAK16	ENSG00000198042.6	1668.67580	791.18900	1.07650	30.94050	29.05560	K562	FXR1
MAK16	ENSG00000198042.6	1342.65820	642.70270	1.06280	30.17570	28.51900	HepG2	UTP3
MAK16	ENSG00000198042.6	493.09950	1423.18430	-1.52920	29.06340	25.26840	K562	MAK16
MAK16	ENSG00000198042.6	1207.62910	2243.11090	-0.89330	23.79590	21.94690	K562	UPF1
MAK16	ENSG00000198042.6	289.67390	610.49070	-1.07550	17.74800	16.42580	HepG2	PCBP1
MAK16	ENSG00000198042.6	473.05050	869.39140	-0.87800	16.88700	15.84490	HepG2	SSB
MAK16	ENSG00000198042.6	999.93340	2041.68400	-1.02990	16.62700	14.45470	K562	FMR1
MAK16	ENSG00000198042.6	455.67520	990.44610	-1.12010	15.70370	14.37360	HepG2	HNRNPLL
MAK16	ENSG00000198042.6	323.05410	626.70260	-0.95600	15.35700	14.02450	HepG2	HNRNPU
MAK16	ENSG00000198042.6	1390.62250	2115.51090	-0.60530	14.78520	13.25000	K562	NSUN2
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 137 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for MAK16 is not available on ENCORE database.

4 RBPs can affect the splicing of MAK16 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
SE	MAK16	ENSG00000198042.6	chr8	+	33347802	33347857	33346505	33346657	33353059	33353134	-0.10700	0.00000	0.00005	excluded	K562	AQR
A3SS	MAK16	ENSG00000198042.6	chr8	+	33354112	33354259	33354142	33354259	33353059	33353134	0.08000	0.00000	0.00000	longer	K562	AQR
SE	MAK16	ENSG00000198042.6	chr8	+	33347802	33347857	33346505	33346657	33353059	33353134	0.05200	0.00001	0.00114	included	HepG2	SFPQ
SE	MAK16	ENSG00000198042.6	chr8	+	33347802	33347857	33346505	33346657	33353059	33353134	-0.09100	0.00020	0.00831	excluded	HepG2	SF3A3
SE	MAK16	ENSG00000198042.6	chr8	+	33347802	33347857	33346505	33346657	33353059	33353134	-0.08100	0.00470	0.07057	excluded	K562	SF3B4

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

MAK16

General Information

Functions

Localization(RBPImage: MAK16)

Domains (AlphaFold: MAK16)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for MAK16 is not avaliable on ENCORE database.

6 proteins are bound to MAK16 RNA:

eCLIP assay for MAK16 is not avaliable on ENCORE database.

Diffrential Expression

2,914 differentially expressed genes after Knockdown/Knockout MAK16 protein :

110 RBPs can affect the gene expression of MAK16 :

The Knockdown/knockout RNA-Seq assay for MAK16 is not available on ENCORE database.

4 RBPs can affect the splicing of MAK16 :

The Knockdown/knockout RNA-Seq assay for MAK16 is not avaliable on ENCORE database.