DDX5

General Information

Gene Name : DDX5
Gene ID : ENSG00000108654.14
Essential Gene : No

Functions

Splicing regulation
RNA stability & decay
microRNA processing

Localization(RBPImage: DDX5)

Nuclei
Nucleoplasm
Cytoplasm
Cytosolic

Domains (AlphaFold: DDX5)

Helicase

Antibody Validation

View validation images for all DDX5 antibodies

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	DDX5	ENSG00000108654.14	574.23	468.69	521.46	ENCSR000AEF
HepG2	DDX5	ENSG00000108654.14	423.87	503.99	463.93	ENCSR985KAT
K562	DDX5	ENSG00000108654.14	418.08	406.29	412.19	ENCSR000AEO

Assays

3 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Controls	Method	Note
knockdown followed by RNA-seq	DDX5-BGHLV30-HepG2	ENCSR808FBR	DDX5	HepG2	Released	ENCSR776SXA	shRNA	KD% = ,
knockdown followed by RNA-seq	DDX5-BG293LV04-WT293		DDX5	293T	Released		shRNA	KD% = ,
knockdown followed by RNA-seq	DDX5-BG293LV06-WT293		DDX5	293T	Released		shRNA	KD% = ,

eCLIP

No eCLIP experiment available

Antibody Validation

GeneName	Antibody_DCC_ID	Product_ID	Lot_ID	HepG2 Primary Validation	K562 Primary Validation	HepG2 Secondary Validation	K562 Secondary Validation
DDX5	ENCAB946VDH	9877	1	Released, ENCODE3		BGHLV30-25, Released, ENCODE3
DDX5	ENCAB374ZWM	A300-523A	3			BGHcLV39-5, Submitted, ENCORE2	BGKcLV37-9, Submitted, ENCORE2
DDX5	ENCAB538ILD	A300-522A	1			BGHcLV39-5, Submitted, ENCORE2	BGKcLV37-9, Submitted, ENCORE2
DDX5	ENCAB749MOE	GTX100234	39274		NotSatisfied, ENCODE3	BGHcLV39-5, Submitted, ENCORE2
DDX5		9877S	2		Submitted, ENCORE2	BGHcLV39-5, Submitted, ENCORE2

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project
DDX5	HepG2	shRNA			BGHLV26-9,BGHLV26-10	BGHLV26-1,BGHLV26-2	redo transfection,redo transfection	NotSatisfied	ENCODE3
DDX5	HepG2	shRNA	ENCSR808FBR	ENCSR776SXA	DDX5_BGHLV30-25B,DDX5_BGHLV30-26B	NT_BGHLV30-1B,NT_BGHLV30-2B	,	Released	ENCODE3
DDX5	K562	CRISPR			DDX5-BGKcLV37-9,DDX5-BGKcLV37-10	NT-BGKcLV37-1,NT-BGKcLV37-2	59.6,64.8	Submitting	ENCORE2
DDX5	HepG2	CRISPR			DDX5-BGHcLV39-5,DDX5-BGHcLV39-6	NT-BGHcLV39-1,NT-BGHcLV39-2	86.6,95.7	Submitting	ENCORE2
DDX5	293T	shRNA			BG293LV01-13,BG293LV01-14	BG293LV01-1,BG293LV01-2		NotSatisfied	ENCORE2
DDX5	293T	shRNA			BG293LV01-15,BG293LV01-16	BG293LV01-1,BG293LV01-2		NotSatisfied	ENCORE2
DDX5	293T	shRNA			BG293LV01-17,BG293LV01-18	BG293LV01-1,BG293LV01-2		NotSatisfied	ENCORE2
DDX5	293T	shRNA			BG293LV04-19,BG293LV04-20	BG293LV04-1,BG293LV04-2		NotSatisfied	ENCORE2
DDX5	293T	shRNA			BG293LV04-21,BG293LV04-22	BG293LV04-1,BG293LV04-2		NotSatisfied	ENCORE2
DDX5	293T	shRNA			DDX5-BG293LV04-23,DDX5-BG293LV04-24	NT-BG293LV04-1,NT-BG293LV04-2	,	Released	ENCORE2
DDX5	293T	shRNA			DDX5-BG293LV06-121B,DDX5-BG293LV06-122B	NT-BG293LV06-1B,NT-BG293LV06-2B	,	Released	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

DDX5: Click to RBP Image Database

Nuclei

Nucleoplasm

Cytoplasm

Cytosolic

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela
DDX5	Product_ID: GTX100234 Lot_ID: 39274 Source: GeneTex Target Name: DDX5-human
DDX5	Product_ID: 9877 Lot_ID: 1 Source: Cell Signaling Technology Target Name: DDX5-human
DDX5	Product_ID: 9877S Lot_ID: 2 Source: Cell Signaling Technology Target Name: DDX5-human
DDX5	Product_ID: A300-523A Lot_ID: 3 Source: Bethyl Labs Target Name: DDX5-human
DDX5	Product_ID: A300-522A Lot_ID: 1 Source: Bethyl Labs Target Name: DDX5-human

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for DDX5 is not avaliable on ENCORE database.

Proteins bound to RNA

DDX5 RNA is bound by 66 proteins.

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eCLIP assay for DDX5 is not avaliable on ENCORE database.

66 proteins are bound to DDX5 RNA:

Show entries

Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	62500209	62500230	-	3'-UTR,5'-UTR,CDS,intron-Distal,intron-Proximal	4.21089	400.00000	HepG2	BCLAF1
chr17	62496873	62496969	-	3'-UTR,CDS,intron-Distal,intron-Proximal	5.64384	400.00000	K562	AQR
chr17	62498279	62498323	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.55270	400.00000	HepG2	BCLAF1
chr17	62498566	62498597	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.90416	400.00000	HepG2	BCLAF1
chr17	62498323	62498338	-	3'-UTR,CDS,intron-Distal	4.62721	400.00000	HepG2	BCLAF1
chr17	62498338	62498342	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.46172	400.00000	HepG2	BCLAF1
chr17	62498083	62498124	-	intron-Distal,intron-Proximal	6.04645	400.00000	K562	AQR
chr17	62498555	62498559	-	3'-UTR,CDS,intron-Distal,intron-Proximal	4.93214	88.09505	HepG2	BCLAF1
chr17	62496916	62496945	-	intron-Distal,intron-Proximal	3.77241	86.96477	HepG2	U2AF2
chr17	62500181	62500204	-	5'-UTR,CDS,intron-Distal,intron-Proximal	3.88805	85.54467	HepG2	BCLAF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 380 entries

eCLIP assay for DDX5 is not avaliable on ENCORE database.

Diffrential Expression

2,968 differentially expressed genes after Knockdown/Knockout DDX5 protein.

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119 RBPs can affect the levels of DDX5.

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2,968 differentially expressed genes after Knockdown/Knockout DDX5 protein :

Only display 100 entries as example. Please click "View All" button to see all 2,968 entries.

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
PLEKHB1	ENSG00000021300.9	494.40630	1971.33470	-1.99540	59.40100	56.36500	HepG2	DDX5
CRLF1	ENSG00000006016.6	1383.12360	3866.88780	-1.48320	41.77950	38.98080	HepG2	DDX5
CYP51A1	ENSG00000001630.11	8649.60190	4487.15860	0.94670	20.36350	18.01030	HepG2	DDX5
SNAI2	ENSG00000019549.4	82.77570	2.89570	4.83710	19.72210	17.40060	HepG2	DDX5
SEMA3G	ENSG00000010319.2	1238.57300	581.56980	1.09060	17.92570	15.69950	HepG2	DDX5
C8B	ENSG00000021852.8	553.50050	214.47890	1.36760	17.90770	15.68770	HepG2	DDX5
CROT	ENSG00000005469.7	432.95470	156.79090	1.46530	16.80480	14.62780	HepG2	DDX5
SLC38A5	ENSG00000017483.10	434.60150	918.84590	-1.08010	15.51060	13.38010	HepG2	DDX5
ANLN	ENSG00000011426.6	1784.45830	3322.98750	-0.89700	14.97010	12.88390	HepG2	DDX5
PON1	ENSG00000005421.4	63.33000	3.84070	4.04340	13.28050	11.26760	HepG2	DDX5
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 100 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

119 RBPs can affect the gene expression of DDX5 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
DDX5	ENSG00000108654.7	10393.73790	17765.57090	-0.77340	57.29410	54.99420	HepG2	SLBP
DDX5	ENSG00000108654.7	23180.92730	14345.43470	0.69220	51.93080	50.32970	HepG2	ZNF622
DDX5	ENSG00000108654.7	37492.50460	22021.03240	0.76760	51.69870	50.24540	HepG2	SRFBP1
DDX5	ENSG00000108654.7	21242.33510	11559.94580	0.87770	38.50290	36.72640	HepG2	TAF15
DDX5	ENSG00000108654.7	19739.54160	11834.63620	0.73800	36.27320	34.74950	HepG2	PTBP1
DDX5	ENSG00000108654.7	4210.26560	12943.03900	-1.62020	35.62880	32.88380	HepG2	DDX5
DDX5	ENSG00000108654.7	16649.21860	10462.96110	0.67010	33.33400	31.82880	HepG2	SRSF9
DDX5	ENSG00000108654.7	18502.93290	11630.56120	0.66970	30.34870	28.70810	HepG2	PCBP1
DDX5	ENSG00000108654.7	12425.94720	19119.25410	-0.62170	28.69710	27.43870	HepG2	SSB
DDX5	ENSG00000108654.7	18973.24020	12321.19510	0.62270	27.44420	25.91370	HepG2	SF3B4
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 135 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for DDX5 is not available on ENCORE database.

69 RBPs can affect the splicing of DDX5 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	FDR	is_included	Cell Line	Affected by RBP
SE	DDX5	ENSG00000108654.7	chr17	-	62496666	62496891	62495733	62496444	62498127	62498187	-0.06600	0.00030	excluded	K562	TIA1
SE	DDX5	ENSG00000108654.7	chr17	-	62496792	62496891	62495733	62496444	62498127	62498187	-0.08000	0.00000	excluded	HepG2	U2AF1
SE	DDX5	ENSG00000108654.7	chr17	-	62496792	62496891	62496031	62496444	62498127	62498187	-0.05600	0.00014	excluded	HepG2	HNRNPM
SE	DDX5	ENSG00000108654.7	chr17	-	62496792	62496891	62495733	62496444	62498127	62498187	-0.12000	0.00126	excluded	K562	LIN28B
SE	DDX5	ENSG00000108654.7	chr17	-	62496792	62496891	62495733	62496444	62498127	62498187	-0.12800	0.00000	excluded	HepG2	U2AF2
SE	DDX5	ENSG00000108654.7	chr17	-	62496666	62496891	62496031	62496444	62498127	62498187	-0.05100	0.00000	excluded	K562	U2AF1
SE	DDX5	ENSG00000108654.7	chr17	-	62496666	62496891	62495733	62496444	62498127	62498187	-0.18100	0.00000	excluded	K562	TARDBP
A3SS	DDX5	ENSG00000108654.7	chr17	-	62496792	62497000	62496792	62496891	62498127	62498187	0.14200	0.00000	longer	HepG2	QKI
A3SS	DDX5	ENSG00000108654.7	chr17	-	62496792	62497000	62496792	62496891	62498127	62498187	-0.09300	0.00404	shorter	K562	CPSF6
A3SS	DDX5	ENSG00000108654.7	chr17	-	62496792	62497000	62496792	62496891	62498127	62498187	-0.35000	0.00000	shorter	K562	FXR1

Showing 1 to 10 of 160 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

DDX5

General Information

Functions

Localization(RBPImage: DDX5)

Domains (AlphaFold: DDX5)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for DDX5 is not avaliable on ENCORE database.

66 proteins are bound to DDX5 RNA:

eCLIP assay for DDX5 is not avaliable on ENCORE database.

Diffrential Expression

2,968 differentially expressed genes after Knockdown/Knockout DDX5 protein :

119 RBPs can affect the gene expression of DDX5 :

The Knockdown/knockout RNA-Seq assay for DDX5 is not available on ENCORE database.

69 RBPs can affect the splicing of DDX5 :

The Knockdown/knockout RNA-Seq assay for DDX5 is not avaliable on ENCORE database.