Antibodies of DDX1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
DDX1Product_ID: 703141
Lot_ID: None
Source: Thermo Fisher
Target Name: DDX1-human		 ThermoFisher_703141_Unknown_DDX1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the DDX1 specific antibody, 703141. Lanes 1 and 3 are 2.5% of five million whole cell lysate input. Lanes 2 and 4 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the DDX1-specific antibody, 703141. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 DDX1-K562-703141.png<br>Caption: Western blot following shRNA against DDX1 in K562 whole cell lysate using DDX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNA against DDX1. DDX1 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
DDX1Product_ID: GTX105205
Lot_ID: 39904
Source: GeneTex
Target Name: DDX1-human		 DDX1_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX1 in K562 and HepG2 whole cell lysate using DDX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX1. Lanes 5-8 follow the same pattern, but in HepG2. DDX1 protein appears as the green band, Tubulin serves as a control and appears in red.
 DDX1_Secondary_Western.png<br>Caption: Western blot following shRNA against DDX1 in K562 and HepG2 whole cell lysate using DDX1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against DDX1. Lanes 5-8 follow the same pattern, but in HepG2. DDX1 protein appears as the green band, Tubulin serves as a control and appears in red.