Caption: IP-WB analysis of 8482S whole cell lysate using the IGF2BP1 specific antibody, 8482S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the IGF2BP1-specific antibody, 8482S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used. Method: immunoprecipitation Releated Sample: eCLIP:220 Status: Submitted Lab: Yeo Lab |