IGF2BP1      CRISPR in K562      Batch: BGKcLV44

Experiment Information (Status: NotSatisfied)
BGKcLV44-101BGKcLV44-102
idx00
TRCN#_or_BGC#BGC#0000002BGC#0000002
shRNA_or_gRNA_sequenceCAAATCCGGCTACGCCTTCGCAAATCCGGCTACGCCTTCG
PAMtggtgg
NameIGF2BP1-C2-AIGF2BP1-C2-A
Sample_IDBGKcLV44-101BGKcLV44-102
transduction_Date7/30/247/30/24
daysD6D6
RBP_nameIGF2BP1IGF2BP1
qPCR_result
Ave_qPCR
RT-qPCR_primer-F
RT-qPCR_primer-R
protein_conc39524066
WB_result54.659.0
Ave_WB54.6
WB_DONE_date8/28/24FOR wb
MW63kDa
IP
antibody_Cat#8482Slot # 2
Antibody DCC ID
submitted_to_DCC_date
Rep1_TPM00
Rep2_TPM00
Action
Library_start_date
repeat_library
Note
ID60056006




Western Blot
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
IGF2BP1Product_ID: 8482S
Lot_ID: 2
Source: Cell Signaling Technology
Target Name: IGF2BP1-human		 CST_8482S_2_IGF2BP1.png<br>Caption: IP-WB analysis of 8482S whole cell lysate using the IGF2BP1 specific antibody, 8482S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the IGF2BP1-specific antibody, 8482S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 IGF2BP1-K562-CRISPR-8482S.png<br>Caption: Western blot following CRISPR against IGF2BP1 in K562 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. IGF2BP1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.




Experiment Status
BGKcLV44-101BGKcLV44-102
Sample_IDBGKcLV44-101BGKcLV44-102
Sample Name
Sample Name
RBPIGF2BP1IGF2BP1
Cell_LineK562K562
Exp UID
StatusNotSatisfiedNotSatisfied
Status_date2024-10-082024-10-08
ProjectENCORE2ENCORE2
Note
ID1368413685




Library-Prep Information
Library info was not avaliable




Sequencing Information
Sequencing info was not avaliable




Data Submission Information
DCC submission information was not avaliable




File Information
Files of the experiment was not avaliable in the database