Antibodies of IGF2BP1


2 antibodies are included.     

 Released    NotSatisfied    Submitted    NotReviewed    OnHold    Submitting    Experimented   
RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
IGF2BP1Product_ID: RN007P
Lot_ID: 3
Source: MBLI
Target Name: IGF2BP1-human		 HepG2_MBLI_RN007P_003_IGF2BP1.png<br>Caption: IP-WB analysis of HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 1% twenty million whole cell lysate input, lane 2 is 1% of the supernatant afer IP and Lane 3 is 10% of IP enrichment using rabbit polyclonal anti-IGF2BP1 Antibody. Lane 4 is 1% ten million whole cell lysate input and lane 5 is 25% IP enrichment using rabbit normal IgG (lanes under 'IgG').
 IGF2BP1_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
IGF2BP1_CRISPR.png<br>Caption: Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
 K562_MBLI_RN007P_003_IGF2BP1.png<br>Caption: IP-Western Blot analysis of K562 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is 2.5% of five million whole cell lysate input, lane 2 is 2.5% of supernatant after immunoprecipitation and Lane 3 is 50% of IP enrichment using either rabbit polyclonal anti-IGF2BP1 antibody (lanes under 'IGF2BP1') or using rabbit normal IgG (lanes under 'IgG').
 IGF2BP1_Secondary_Western.png<br>Caption: Western blot following shRNA against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different shRNAs against IGF2BP1. Lanes 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
IGF2BP1_CRISPR.png<br>Caption: Western blot following CRISPR against IGF2BP1 in K562 and HepG2 whole cell lysate using IGF2BP1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against IGF2BP1. 5-8 follow the same pattern, but in HepG2. IGF2BP1 protein appears as the green band, Tubulin serves as a control and appears in red.
IGF2BP1Product_ID: 8482S
Lot_ID: 2
Source: Cell Signaling Technology
Target Name: IGF2BP1-human		 CST_8482S_2_IGF2BP1.png<br>Caption: IP-WB analysis of 8482S whole cell lysate using the IGF2BP1 specific antibody, 8482S. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the IGF2BP1-specific antibody, 8482S. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.