RAE1

General Information

Gene Name       : RAE1
Gene ID             : ENSG00000101146.12
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RAE1)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RAE1ENSG00000101146.1231.5537.0134.28ENCSR000AEF
HepG2RAE1ENSG00000101146.1289.5981.1185.35ENCSR985KAT
K562RAE1ENSG00000101146.1275.0367.5771.30ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RAE1-BGKcLV17-K562ENCSR092QTFRAE1K562ReleasedENCSR516TLX   CRISPRKD% = 68.9,69.3
knockdown followed by RNA-seq RAE1-BGHcLV18-HepG2ENCSR723QPDRAE1HepG2ReleasedENCSR670KAS   CRISPRKD% = 61.5,68.3






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RAE1ENCAB079EVOPA5-76998TL2693074Released, ENCODE4BGHcLV18-39, Released, ENCODE4BGKcLV17-43, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RAE1K562CRISPRENCSR092QTFENCSR516TLXRAE1-BGKcLV17-43,RAE1-BGKcLV17-44NT-BGKcLV17-1,NT-BGKcLV17-268.9,69.3ReleasedENCODE4
RAE1HepG2CRISPRENCSR723QPDENCSR670KASRAE1-BGHcLV18-39,RAE1-BGHcLV18-40NT-BGHcLV18-1,NT-BGHcLV18-261.5,68.3ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RAE1Product_ID: PA5-76998
Lot_ID: TL2693074
Source: Thermo Fisher
Target Name: RAE1-human		 RAE1-HEPG2-CRISPR-PA5-76998.png<br>Caption: Western blot following CRISPR against RAE1 in HepG2 whole cell lysate using RAE1 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RAE1. RAE1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_PA5-76998_TL2693074_RAE1.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RAE1 specific antibody, PA5-76998. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the RAE1-specific antibody, PA5-76998. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RAE1-K562-CRISPR-PA5-76998.png<br>Caption: Western blot following CRISPR against RAE1 in K562 whole cell lysate using RAE1 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RAE1. RAE1 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RAE1 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RAE1 RNA is bound by 22 proteins.  







eCLIP assay for RAE1 is not avaliable on ENCORE database.





22 proteins are bound to RAE1 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr205595320055953280+3'-UTR4.226403.98353K562RBM15
chr205595327955953338+3'-UTR3.147866.00087K562YBX3
chr205594969355949799+3'-UTR,CDS4.160453.96931HepG2G3BP1
chr205594984555949900+3'-UTR,CDS,intron-Proximal4.6829417.12423HepG2PRPF8
chr205594966355949730+3'-UTR,CDS3.080343.55800HepG2BCLAF1
chr205594970055949891+3'-UTR,CDS,intron-Proximal3.253418.51590HepG2GRWD1
chr205595351355953595+3'-UTR,polyA_site4.249495.45333HepG2CSTF2
chr205595344255953491+3'-UTR8.1418245.25248K562PABPC4
chr205595320455953283+3'-UTR5.037156.23813HepG2SUB1
chr205592650455926587+5'-UTR,intron-Distal,intron-Proximal5.8638611.24224HepG2RBM22
chr205592644755926530+5'-UTR,intron-Distal,intron-Proximal3.9529210.01959HepG2PRPF8
chr205592633555926434+5'-UTR,intron-Proximal4.1252513.37398K562DDX3X
chr205592630855926356+5'-UTR3.112903.40404HepG2NCBP2
chr205592631455926383+5'-UTR4.106334.12025K562YBX3
chr205594985755949901+CDS,intron-Proximal5.150676.22530K562AQR
chr205593149955931559+CDS,intron-Proximal3.203124.99842HepG2PPIG
chr205593158155931633+CDS,intron-Proximal5.083455.01842HepG2PRPF8
chr205594044855940540+CDS,intron-Proximal4.502465.52476K562BUD13
chr205592983855929947+CDS,intron-Proximal5.158315.43043K562EFTUD2
chr205594851855948562+CDS,intron-Distal,intron-Proximal3.540373.95921HepG2U2AF2
chr205594041155940486+CDS,intron-Proximal3.165305.40132HepG2BCLAF1
chr205592917855929221+CDS,intron-Proximal4.337775.05356HepG2PRPF8
chr205594049355940540+CDS,intron-Proximal4.213194.55323HepG2BUD13
chr205592910155929173+CDS4.006213.70678K562BUD13
chr205594375555943888+CDS,intron-Proximal3.366157.20311HepG2PPIG
chr205594851755948581+CDS,intron-Distal,intron-Proximal4.339933.83824K562BUD13
chr205594046555940552+CDS,intron-Proximal5.107706.35567HepG2PRPF8
chr205594854155948638+CDS,intron-Distal,intron-Proximal3.245423.77614HepG2G3BP1
chr205592977155929877+CDS,intron-Proximal4.733454.89631K562SND1
chr205594191355942002+CDS,intron-Proximal4.6178112.81328HepG2PRPF8
chr205594041055940477+CDS,intron-Proximal3.081674.35521HepG2BUD13
chr205594182855941885+CDS,intron-Proximal4.085045.47926K562AQR
chr205592977455929873+CDS,intron-Proximal3.329024.53005K562YBX3
chr205594205955942138+CDS,intron-Proximal3.191186.28230HepG2PPIG
chr205594049255940545+CDS,intron-Proximal4.930477.09786HepG2AQR
chr205594639055946451+intron-Distal4.130113.38968HepG2BCLAF1
chr205594810555948178+intron-Distal3.063833.19398HepG2XPO5
chr205595074955950903+intron-Distal3.203583.84715HepG2HNRNPK
chr205594513555945246+intron-Distal4.012197.07387HepG2HNRNPK
chr205595056955950671+intron-Distal4.243627.22863HepG2PCBP2
chr205594389955943990+intron-Distal,intron-Proximal4.390413.49003K562PRPF8
chr205594961155949661+intron-Proximal5.168457.71578HepG2SF3B4
chr205594049855940548+intron-Proximal5.4988416.41423K562AQR
chr205594880655948894+intron-Proximal3.430983.76553K562PRPF8
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RAE1 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RAE1 is not available on ENCORE database.

  • 67 RBPs can affect the levels of RAE1.  








The Knockdown/knockout RNA-Seq assay for RAE1 is not available on ENCORE database.





67 RBPs can affect the gene expression of RAE1 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RAE1ENSG00000101146.81343.346401047.414900.358904.973104.20380HepG2ACO1
RAE1ENSG00000101146.81239.751701052.272200.236402.173901.48440HepG2AKAP8L
RAE1ENSG00000101146.8785.993801055.91700-0.425902.596901.72460HepG2BCLAF1
RAE1ENSG00000101146.8635.74060887.82310-0.481804.035503.32850HepG2BOP1
RAE1ENSG00000101146.81299.989301047.110600.312004.091203.31190HepG2CKAP4
RAE1ENSG00000101146.81334.75280948.066500.493402.551401.81810HepG2CNOT7
RAE1ENSG00000101146.81236.988601655.18470-0.420207.608306.46900HepG2DAZAP1
RAE1ENSG00000101146.8757.959401016.04910-0.422803.550402.44700HepG2DHX30
RAE1ENSG00000101146.8765.171801053.43160-0.461205.572504.60430HepG2DNAJC21
RAE1ENSG00000101146.8851.393101044.19410-0.294502.131701.54530HepG2DNAJC2
RAE1ENSG00000101146.8738.392901062.25270-0.524704.135003.41840HepG2EIF2S1
RAE1ENSG00000101146.8990.26080742.010900.416303.034902.09200HepG2EIF3D
RAE1ENSG00000101146.81268.931401633.06440-0.364004.782703.73110HepG2EIF4G1
RAE1ENSG00000101146.81233.877201564.34910-0.342402.525501.74550HepG2EIF4G2
RAE1ENSG00000101146.81350.582101023.443400.400103.879302.88900HepG2EWSR1
RAE1ENSG00000101146.81259.695301521.23110-0.272202.428101.54490HepG2HNRNPA2B1
RAE1ENSG00000101146.81165.837301416.58670-0.281103.087102.33250HepG2HNRNPF
RAE1ENSG00000101146.81129.169701394.93250-0.304902.673501.96130HepG2IGF2BP1
RAE1ENSG00000101146.8985.033601200.39660-0.285303.599402.77300HepG2ILF2
RAE1ENSG00000101146.81863.731401184.961900.653307.132806.09530HepG2MAGOH
RAE1ENSG00000101146.81711.116201191.565300.522004.236703.47230HepG2MATR3
RAE1ENSG00000101146.8565.95020781.80460-0.466103.198102.39560HepG2NSUN2
RAE1ENSG00000101146.8772.91720613.627600.332902.504401.33910HepG2PKM
RAE1ENSG00000101146.8878.137101048.15660-0.255302.070201.42490HepG2PPIL4
RAE1ENSG00000101146.81160.04970921.745100.331602.183401.46330HepG2PRPF8
RAE1ENSG00000101146.81047.731501240.41970-0.243601.869501.40260HepG2PTBP1
RAE1ENSG00000101146.8997.231601566.79010-0.651808.835007.85060HepG2PUF60
RAE1ENSG00000101146.81949.475601701.015000.196601.884401.43550HepG2RBM22
RAE1ENSG00000101146.8908.99650722.028800.332102.348501.77700HepG2RPS19
RAE1ENSG00000101146.8880.100601046.53080-0.249902.387601.78240HepG2SAFB2
RAE1ENSG00000101146.82498.949401194.393801.0649013.4724011.91790HepG2SF3A3
RAE1ENSG00000101146.81553.595301291.406600.266602.852802.25330HepG2SF3B4
RAE1ENSG00000101146.81163.349901502.40250-0.369005.527604.59320HepG2SLBP
RAE1ENSG00000101146.82350.575201921.228000.290904.464203.87150HepG2SRFBP1
RAE1ENSG00000101146.81974.112301347.961300.5503011.6474010.82020HepG2SRSF1
RAE1ENSG00000101146.81161.806301391.75330-0.260502.798302.09860HepG2SRSF7
RAE1ENSG00000101146.8914.544301211.63470-0.405804.648703.95120HepG2TAF15
RAE1ENSG00000101146.8665.24080859.51250-0.369602.467801.77740HepG2TARDBP
RAE1ENSG00000101146.8865.500501152.11310-0.412705.527904.78250HepG2TRIM56
RAE1ENSG00000101146.81636.961001071.708200.611007.008706.01810HepG2U2AF1
RAE1ENSG00000101146.81941.199901022.657300.9245029.8917028.24130HepG2UTP3
RAE1ENSG00000101146.8974.950601146.77520-0.234202.307401.68710HepG2ZC3H8
RAE1ENSG00000101146.82035.018501433.968700.504909.823808.83680K562AGGF1
RAE1ENSG00000101146.81888.429401492.096200.339702.908202.23860K562AKAP8L
RAE1ENSG00000101146.84191.894702630.364100.6722010.688309.64660K562AQR
RAE1ENSG00000101146.81387.261601605.34760-0.210602.208001.34850K562BCLAF1
RAE1ENSG00000101146.8550.258501218.63050-1.1471018.4588016.19630K562BOP1
RAE1ENSG00000101146.8658.66330854.23050-0.375102.393701.42780K562CCAR1
RAE1ENSG00000101146.8884.829101108.97470-0.325802.573701.56960K562DDX3X
RAE1ENSG00000101146.83200.674702267.346000.4973011.6283010.39300K562DDX47
RAE1ENSG00000101146.82012.151401605.685000.325402.378201.57480K562DKC1
RAE1ENSG00000101146.81694.604101306.449900.375203.050002.08960K562EFTUD2
RAE1ENSG00000101146.81749.244502195.73260-0.328004.388903.51110K562EIF2S2
RAE1ENSG00000101146.81489.523101121.922300.408805.369504.27530K562EIF3G
RAE1ENSG00000101146.82807.988503279.39070-0.223902.187901.34770K562EIF4G2
RAE1ENSG00000101146.8817.743101117.49280-0.450503.255202.25680K562FTO
RAE1ENSG00000101146.81832.368101204.889800.6047011.8120010.65060K562FXR1
RAE1ENSG00000101146.81004.655901237.09250-0.300302.126801.42720K562GPKOW
RAE1ENSG00000101146.81946.194701583.533200.297402.133701.41920K562KIF1C
RAE1ENSG00000101146.8669.770201056.36280-0.657402.873501.91850K562KRR1
RAE1ENSG00000101146.81968.424601416.573300.474505.857804.61870K562MAGOH
RAE1ENSG00000101146.81793.900301243.406900.528703.809402.72990K562NAA15
RAE1ENSG00000101146.81695.488301913.88740-0.174802.070001.41390K562NSUN2
RAE1ENSG00000101146.82093.423601193.040900.811104.304302.99710K562PABPN1
RAE1ENSG00000101146.81767.039601260.432900.487305.367003.99510K562PCBP2
RAE1ENSG00000101146.82564.844401271.326801.0124019.2160017.21810K562PRPF8
RAE1ENSG00000101146.81761.929501376.276000.356302.571701.92790K562PTBP1
RAE1ENSG00000101146.82201.599801479.844300.573006.646205.84640K562PUS1
RAE1ENSG00000101146.81932.606801441.807200.422605.711004.88490K562RBM15
RAE1ENSG00000101146.81688.140401319.784400.355003.034802.14950K562RBM22
RAE1ENSG00000101146.81886.301101294.971000.542504.897703.80960K562RPS19
RAE1ENSG00000101146.81916.252901091.038400.812503.717502.25860K562SF1
RAE1ENSG00000101146.81982.460801233.303900.6847012.6697010.75110K562SF3B4
RAE1ENSG00000101146.82013.950201253.034400.684502.869601.61360K562SNRNP200
RAE1ENSG00000101146.82087.572901349.188800.6296010.070508.73450K562SRSF1
RAE1ENSG00000101146.81017.215501273.20730-0.323802.570501.56240K562SRSF4
RAE1ENSG00000101146.81596.790102228.14820-0.480703.202301.83650K562STAU1
RAE1ENSG00000101146.81933.127301518.187800.348503.848202.87870K562SYNCRIP
RAE1ENSG00000101146.81632.744001149.650400.506004.168602.76820K562U2AF1
RAE1ENSG00000101146.81502.731501187.408700.339703.339402.45950K562U2AF2
RAE1ENSG00000101146.82446.367502963.29520-0.276602.375801.58520K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RAE1 is not available on ENCORE database.





3 RBPs can affect the splicing of RAE1 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERAE1ENSG00000101146.8chr20+5595123455951322559496625594985755953068559542670.051000.000000.00000includedK562SRSF1
SERAE1ENSG00000101146.8chr20+559485305594863755943625559438685594871855948794-0.115000.000000.00000excludedK562AQR
SERAE1ENSG00000101146.8chr20+5595123455951322559496625594985755953068559542670.052000.000040.00299includedHepG2MATR3
SERAE1ENSG00000101146.8chr20+5595123455951322559496625594985755953068559542670.112000.000000.00000includedHepG2SRSF1
A3SSRAE1ENSG00000101146.8chr20+5595304155953155559530685595315555949662559498570.208000.000000.00000longerK562AQR
A3SSRAE1ENSG00000101146.8chr20+5594370255943868559437605594386855942063559421350.095000.000000.00000longerK562AQR
A5SSRAE1ENSG00000101146.8chr20+5594376055943963559437605594386855948530559486370.079000.000000.00000longerK562AQR
MXERAE1ENSG00000101146.8chr20+55943713559438685594853055948637559420635594213555948718559487940.112000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943713559438685594847655948637559420635594213555948718559487940.112000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943702559438685594853055948637559420635594213555948718559487940.137000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943702559438685594847655948637559420635594213555948718559487940.137000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943732559438685594853055948637559420635594213555948718559487940.129000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943732559438685594847655948637559420635594213555948718559487940.129000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943664559438685594853055948637559420635594213555948718559487940.107000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943664559438685594847655948637559420635594213555948718559487940.107000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943648559438685594853055948637559420635594213555948718559487940.106000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943648559438685594847655948637559420635594213555948718559487940.106000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943625559438685594853055948637559420635594213555948718559487940.112000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943625559438685594847655948637559420635594213555948718559487940.112000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943632559438685594853055948637559420635594213555948718559487940.108000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+55943632559438685594847655948637559420635594213555948718559487940.108000.000000.000001includedK562AQR
MXERAE1ENSG00000101146.8chr20+5594853055948637559487185594879455943760559438685594966255949857-0.121000.000110.001312includedK562AQR
MXERAE1ENSG00000101146.8chr20+5594853055948637559486635594879455943648559438685594966255949857-0.107000.000910.008392includedK562AQR
MXERAE1ENSG00000101146.8chr20+5594853055948637559486895594879455943648559438685594966255949857-0.107000.000940.008632includedK562AQR

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RAE1 is not avaliable on ENCORE database.