GRB2

General Information

Gene Name : GRB2
Gene ID : ENSG00000177885.14
Essential Gene : Yes

Functions

Not Available

Localization

Not Available

Domains (AlphaFold: GRB2)

Not Available

Antibody Validation

Not Avaiable

Expression Level in Cell lines

Cell Line	Gene Name	Ensembl ID	Rep1 TPM	Rep2 TPM	Average TPM	Experiment ID
Gm12878	GRB2	ENSG00000177885.14	126.62	96.24	111.43	ENCSR000AEF
HepG2	GRB2	ENSG00000177885.14	80.30	127.39	103.84	ENCSR985KAT
K562	GRB2	ENSG00000177885.14	60.31	57.01	58.66	ENCSR000AEO

Assays

1 Released experiments are available on ENCORE database.

Assay Type	Exp_Name	ENCODE ID	Target label	Biosample	Status	Linked Antibody	Controls	Method	Note
knockdown followed by RNA-seq	GRB2-BG293LV06-WT293		GRB2	293T	Released			shRNA	KD% = ,

eCLIP

No eCLIP experiment available

Antibody Validation

No Antibody Primary Validation image or Secondary image available

KD RNA-Seq

GeneName	Cell_Line	Method	Exp_ID	Control_ID	Sample_reps	Control_reps	KD%	Status	Project	Note
GRB2	293T	shRNA			GRB2-BG293LV02-17,GRB2-BG293LV02-18	NT-BG293LV02-1,NT-BG293LV02-2	,	Revoked	ENCORE2
GRB2	293T	shRNA			GRB2-BG293LV06-7,GRB2-BG293LV06-8	NT-BG293LV06-1,NT-BG293LV06-2	,	Released	ENCORE2

RNA Bind-n-Seqn

No RBNS experiment available

Localization Image in HepG2

No Localization Information available

RBP	Antibody Info	Primary-HepG2	Secondary-HepG2	Primary-K562	Secondary-K562	Primary-UBERON	Primary-Hela

Summary
Proteins Bound to RBP RNA

RNA Targets

eCLIP assay for GRB2 is not avaliable on ENCORE database.

Proteins bound to RNA

GRB2 RNA is bound by 37 proteins.

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eCLIP assay for GRB2 is not avaliable on ENCORE database.

37 proteins are bound to GRB2 RNA:

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Search:

Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name
chr17	73315267	73315322	-	3'-UTR,intron-Distal	5.76777	400.00000	K562	PUM2
chr17	73315112	73315229	-	3'-UTR,intron-Distal	6.41201	400.00000	K562	UPF1
chr17	73315278	73315320	-	3'-UTR,intron-Distal	4.86224	400.00000	K562	UPF1
chr17	73315790	73315902	-	3'-UTR,intron-Distal	6.67618	78.50819	HepG2	UPF1
chr17	73314252	73314319	-	3'-UTR,intron-Distal	4.70943	67.85805	K562	PUM2
chr17	73314173	73314252	-	3'-UTR,intron-Distal	5.23528	62.41502	K562	PUM2
chr17	73315324	73315340	-	3'-UTR,intron-Distal	4.63654	51.65748	K562	UPF1
chr17	73315340	73315375	-	3'-UTR,intron-Distal	4.93636	50.47957	K562	UPF1
chr17	73314723	73314795	-	3'-UTR,intron-Distal,intron-Proximal	5.49294	49.43609	K562	UPF1
chr17	73315858	73315899	-	3'-UTR,intron-Distal	5.57283	49.43609	K562	UPF1
Chr	Start	End	Strand	Matched Area	log2(fold change)	-log(p-value)	Cell Line	Binding Protein Name

Showing 1 to 10 of 173 entries

eCLIP assay for GRB2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout assay for GRB2 is not available on ENCORE database.
44 RBPs can affect the levels of GRB2.

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The Knockdown/knockout RNA-Seq assay for GRB2 is not available on ENCORE database.

44 RBPs can affect the gene expression of GRB2 :

Show entries

Search:

Gene Name	Gene ID	KDMean	CNMean	log2(fold change) *	-log(pval) **	-log(padj) **	Cell Line	Affected by RBP	Bound by RBP
GRB2	ENSG00000177885.9	1064.22240	2258.48600	-1.08560	26.57940	24.23200	HepG2	HNRNPM
GRB2	ENSG00000177885.9	2893.91820	4282.11630	-0.56530	15.04680	14.12600	HepG2	SRFBP1
GRB2	ENSG00000177885.9	4542.88830	3303.59400	0.45950	12.25210	11.15180	K562	AGGF1
GRB2	ENSG00000177885.9	5483.57890	3408.82910	0.68570	11.74060	10.67040	K562	PUS1
GRB2	ENSG00000177885.9	1125.92350	2082.89750	-0.88750	12.10210	9.81750	K562	HNRNPM
GRB2	ENSG00000177885.9	1434.13700	2208.67480	-0.62300	9.62160	8.56300	K562	HNRNPC
GRB2	ENSG00000177885.9	2047.91510	2830.25610	-0.46680	8.58350	7.68570	HepG2	TAF15
GRB2	ENSG00000177885.9	4071.81720	3148.86310	0.37070	8.04170	7.33230	HepG2	SRSF1
GRB2	ENSG00000177885.9	1562.78920	2539.13820	-0.70020	7.96350	6.91070	HepG2	SUPV3L1
GRB2	ENSG00000177885.9	1545.53160	2594.22100	-0.74720	8.45720	6.77780	HepG2	BCCIP
Gene Name	Gene ID	KDMean	CNMean	log2(fold change)	-log(pval)	-log(padj)	Cell Line	Affected by RBP	Bound by RBP

Showing 1 to 10 of 52 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

The Knockdown/knockout RNA-Seq assay for GRB2 is not available on ENCORE database.

3 RBPs can affect the splicing of GRB2 :

Show entries

Search:

Event Type	Gene Name	Gene ID	chr	Strand	ExonStart	ExonEnd	2ndExonStart	2ndExonEnd	upstreamES	upstreamEE	downstreamES	downstreamEE	dPSI	p-value	FDR	is_included	Cell Line	Affected by RBP
A3SS	GRB2	ENSG00000177885.9	chr17	-	73328780	73328967			73328780	73328878	73389631	73389945	0.07600	0.00000	0.00000	longer	K562	AQR
A3SS	GRB2	ENSG00000177885.9	chr17	-	73317748	73317947			73317748	73317908	73321978	73322101	0.12600	0.00000	0.00000	longer	K562	AQR
MXE	GRB2	ENSG00000177885.9	chr17	-	73321978	73322101	73328780	73328924	73317748	73317908	73389631	73389846	0.06400	0.00000	0.00000	1included	K562	AQR
SE	GRB2	ENSG00000177885.9	chr17	-	73316412	73316634			73314156	73314378	73317739	73317908	-0.06000	0.00016	0.00892	excluded	K562	TARDBP
SE	GRB2	ENSG00000177885.9	chr17	-	73316412	73316634			73314835	73314877	73317739	73317908	-0.05200	0.00146	0.04963	excluded	K562	PRPF8

Showing 1 to 5 of 5 entries

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".

GRB2

General Information

Functions

Localization

Domains (AlphaFold: GRB2)

Antibody Validation

Expression Level in Cell lines

Assays

RNA Targets

Proteins bound to RNA

eCLIP assay for GRB2 is not avaliable on ENCORE database.

37 proteins are bound to GRB2 RNA:

eCLIP assay for GRB2 is not avaliable on ENCORE database.

Diffrential Expression

The Knockdown/knockout RNA-Seq assay for GRB2 is not available on ENCORE database.

44 RBPs can affect the gene expression of GRB2 :

The Knockdown/knockout RNA-Seq assay for GRB2 is not available on ENCORE database.

3 RBPs can affect the splicing of GRB2 :

The Knockdown/knockout RNA-Seq assay for GRB2 is not avaliable on ENCORE database.