MECP2

General Information

Gene Name       : MECP2
Gene ID             : ENSG00000169057.21
Essential Gene : No

Functions

Localization

Domains (AlphaFold: MECP2)


Antibody Validation

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878MECP2ENSG00000169057.215.028.456.73ENCSR000AEF
HepG2MECP2ENSG00000169057.218.357.958.15ENCSR985KAT
K562MECP2ENSG00000169057.2121.8120.5321.17ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq MECP2-BGKcLV15-K562ENCSR665DMAMECP2K562ReleasedENCSR404OHQ   CRISPRKD% = 55.2,54.7
knockdown followed by RNA-seq MECP2-BGHcLV16-HepG2ENCSR266XKNMECP2HepG2ReleasedENCSR964HKT   CRISPRKD% = 58.4,58.2






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
MECP2ENCAB380QZE49-1029A200042181226Released, ENCODE4BGHcLV16-33, Released, ENCODE4BGKcLV15-39, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
MECP2K562CRISPRENCSR665DMAENCSR404OHQMECP2-BGKcLV15-39,MECP2-BGKcLV15-40NT-BGKcLV15-1,NT-BGKcLV15-255.2,54.7ReleasedENCODE4
MECP2HepG2CRISPRENCSR266XKNENCSR964HKTMECP2-BGHcLV16-33,MECP2-BGHcLV16-34NT-BGHcLV16-1,NT-BGHcLV16-258.4,58.2ReleasedENCODE4
MECP2HepG2CRISPRBGHcLV16-35,BGHcLV16-36BGHcLV16-1,BGHcLV16-231.1,27.1NotSatisfiedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
MECP2Product_ID: 49-1029
Lot_ID: A200042181226
Source: Thermo Fisher
Target Name: MECP2-human		 MECP2-HEPG2-CRISPR-49-1029.png<br>Caption: Western blot following CRISPR against MECP2 in HepG2 whole cell lysate using MECP2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MECP2. MECP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.
 ThermoFisher_49-1029_A200042181226_MECP2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the MECP2 specific antibody, 49-1029. Lane 1 is 2.5% of five million whole cell lysate input. Lanes 2 and 3 are 50% of IP enrichment from five million whole cell lysate using normal IgG antibody and the MECP2-specific antibody, 49-1029. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 MECP2-K562-CRISPR-49-1029.png<br>Caption: Western blot following CRISPR against MECP2 in K562 whole cell lysate using MECP2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against MECP2. MECP2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for MECP2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • MECP2 RNA is bound by 24 proteins.  







eCLIP assay for MECP2 is not avaliable on ENCORE database.





24 proteins are bound to MECP2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chrX153323998153324034-3'-UTR,intron-Distal,intron-Proximal4.772814.82767HepG2SF3B4
chrX153290573153290648-3'-UTR,intron-Distal4.546373.81568K562FAM120A
chrX153297724153297788-3'-UTR,CDS,intron-Distal,intron-Proximal3.606333.02250K562YBX3
chrX153294479153294579-3'-UTR,intron-Distal3.247353.71789HepG2PTBP1
chrX153294433153294566-3'-UTR,intron-Distal4.063675.84135HepG2PCBP2
chrX153297964153298007-3'-UTR,CDS,intron-Distal3.331783.73423K562UCHL5
chrX153294668153294733-3'-UTR,intron-Distal5.385066.34419K562PUM2
chrX153294487153294575-3'-UTR,intron-Distal4.509414.69074K562PTBP1
chrX153296860153296922-3'-UTR,CDS,intron-Distal,intron-Proximal3.309433.75245K562BUD13
chrX153292700153292765-3'-UTR,intron-Distal3.425215.67957K562UPF1
chrX153323944153323998-3'-UTR,intron-Distal4.080915.34167HepG2SF3B4
chrX153297843153297944-3'-UTR,CDS,intron-Distal,intron-Proximal4.124269.43617K562UCHL5
chrX153293772153293843-3'-UTR,intron-Distal3.739898.21835K562UPF1
chrX153296890153296960-3'-UTR,CDS,intron-Distal,intron-Proximal4.226958.28159K562SF3B4
chrX153363064153363151-5'-UTR,CDS,intron-Distal,intron-Proximal4.596495.32122HepG2NCBP2
chrX153363055153363143-5'-UTR,CDS,intron-Distal,intron-Proximal3.594933.01533HepG2SRSF1
chrX153362288153362361-5'-UTR,intron-Distal3.580938.11002K562NONO
chrX153362244153362341-5'-UTR,intron-Distal4.300574.22598K562CSTF2T
chrX153362416153362509-5'-UTR,intron-Distal,intron-Proximal3.391046.84977K562NONO
chrX153362240153362288-5'-UTR,intron-Distal3.335214.25264K562NONO
chrX153363058153363159-5'-UTR,CDS,intron-Distal,intron-Proximal3.788735.82148K562DDX3X
chrX153296352153296406-CDS,intron-Distal,intron-Proximal3.170703.46769K562YBX3
chrX153312265153312320-intron-Distal4.092693.24322HepG2MATR3
chrX153326137153326215-intron-Distal4.466864.76505K562HNRNPK
chrX153362086153362167-intron-Distal4.255378.40323K562NONO
chrX153315255153315315-intron-Distal4.174444.05620HepG2MATR3
chrX153312641153312743-intron-Distal5.207258.82993HepG2SUGP2
chrX153327168153327281-intron-Distal3.816116.20952K562HNRNPK
chrX153312264153312321-intron-Distal4.528265.76788K562MATR3
chrX153316066153316118-intron-Distal4.381213.54208HepG2SUGP2
chrX153342683153342806-intron-Distal4.216974.08332K562NONO
chrX153311908153311986-intron-Distal4.3667610.58275HepG2SUGP2
chrX153312021153312119-intron-Distal5.2722410.55100HepG2SUGP2
chrX153316181153316263-intron-Distal4.417074.47026K562MATR3
chrX153316058153316153-intron-Distal4.729206.20726K562HNRNPK
chrX153311509153311569-intron-Distal3.111274.61989HepG2SUGP2
chrX153353330153353420-intron-Distal3.080295.15194K562UPF1
chrX153312019153312155-intron-Distal5.369189.60586K562MATR3
chrX153362567153362594-intron-Distal3.876133.53896K562NONO
chrX153297523153297615-intron-Distal,intron-Proximal4.082314.19412K562EFTUD2
chrX153354676153354841-intron-Distal3.8022910.27184K562KHSRP
chrX153340170153340316-intron-Distal4.986637.23552K562HNRNPK
chrX153311677153311736-intron-Distal4.577196.07481HepG2SUGP2
chrX153354920153354970-intron-Distal3.595735.32141K562UPF1
chrX153312172153312327-intron-Distal4.2778913.27306HepG2SUGP2
chrX153330618153330674-intron-Distal4.121903.79026K562HNRNPM
chrX153356747153356825-intron-Distal5.5875110.67163K562EXOSC5
chrX153301591153301763-intron-Distal4.207333.31570HepG2HNRNPK
chrX153330633153330795-intron-Distal5.075008.56566HepG2SUGP2
chrX153362897153363013-intron-Distal4.073874.10564HepG2CSTF2T
chrX153358252153358303-intron-Distal4.487404.66899HepG2HNRNPM
chrX153351157153351262-intron-Distal4.531775.04993HepG2QKI
chrX153361761153361815-intron-Distal3.620644.94042K562NONO
chrX153315273153315374-intron-Distal3.883755.60995K562MATR3
chrX153326396153326476-intron-Distal4.231874.10192HepG2HNRNPK
chrX153330675153330765-intron-Distal4.478285.01910HepG2MATR3
chrX153317548153317627-intron-Distal3.868963.24322HepG2MATR3
chrX153311890153311969-intron-Distal4.575655.57532K562HNRNPM
chrX153312155153312242-intron-Distal4.807756.75776K562MATR3
chrX153316410153316473-intron-Distal4.265743.27829HepG2MATR3
chrX153314332153314404-intron-Distal3.748634.57279K562PTBP1
chrX153308693153308742-intron-Distal4.438163.57189K562ILF3
chrX153362943153362995-intron-Distal4.811513.62726K562DROSHA
chrX153312947153313012-intron-Distal4.061823.90241K562MATR3
chrX153330739153330800-intron-Distal3.479674.56666K562HNRNPM
chrX153312028153312091-intron-Distal4.314493.78432HepG2MATR3
chrX153362933153363022-intron-Distal4.589256.08649K562CSTF2T
chrX153312694153312739-intron-Distal3.931733.24322HepG2MATR3
chrX153330442153330543-intron-Distal4.383754.24113K562MATR3
chrX153354970153355015-intron-Distal3.837854.82552K562UPF1
chrX153362003153362067-intron-Distal3.784695.85821K562NONO
chrX153349209153349271-intron-Distal3.865803.57018HepG2HNRNPK
chrX153311843153311908-intron-Distal4.055047.03506HepG2SUGP2
chrX153312389153312470-intron-Distal3.446514.26619K562MATR3
chrX153362915153363002-intron-Distal4.923785.40320K562NONO
chrX153330678153330739-intron-Distal4.315234.51377K562HNRNPM
chrX153340387153340477-intron-Distal4.135773.06015K562MATR3
chrX153326884153326937-intron-Distal4.113233.98115K562MATR3
chrX153312108153312193-intron-Distal5.022015.37222HepG2MATR3
chrX153349185153349274-intron-Distal4.276034.57222K562HNRNPK
chrX153327133153327220-intron-Distal3.997563.33944K562MATR3
chrX153353246153353370-intron-Distal3.292267.47863K562KHSRP
chrX153312350153312401-intron-Distal4.339984.28663HepG2SUGP2
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for MECP2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for MECP2 is not available on ENCORE database.

  • 69 RBPs can affect the levels of MECP2.  








The Knockdown/knockout RNA-Seq assay for MECP2 is not available on ENCORE database.





69 RBPs can affect the gene expression of MECP2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
MECP2ENSG00000169057.15677.49660875.99440-0.370701.959201.50250HepG2AKAP1
MECP2ENSG00000169057.15961.87390749.192200.360402.547901.97030HepG2BOP1
MECP2ENSG00000169057.15583.66040722.06300-0.307002.166301.58640HepG2CELF1
MECP2ENSG00000169057.15394.43990578.88110-0.553504.220403.40040HepG2DDX21
MECP2ENSG00000169057.151205.80720839.039700.523104.210503.45190HepG2DDX3X
MECP2ENSG00000169057.15616.44920791.46250-0.360502.291401.37230HepG2DDX5
MECP2ENSG00000169057.15930.52570692.869700.425402.733102.14390HepG2EIF2S1
MECP2ENSG00000169057.151409.90440765.593200.8808010.029808.83050HepG2EIF4A3
MECP2ENSG00000169057.151078.45370745.621000.532303.169002.43540HepG2EIF4B
MECP2ENSG00000169057.15912.23470733.711700.314102.129801.40960HepG2EIF4G2
MECP2ENSG00000169057.151563.512301314.154100.250602.324301.76470HepG2ETF1
MECP2ENSG00000169057.15954.61660777.688700.295602.058601.33980HepG2EWSR1
MECP2ENSG00000169057.15742.35920513.920600.530504.536403.54420HepG2FUBP3
MECP2ENSG00000169057.151037.03470761.090800.446203.276202.30180HepG2G3BP2
MECP2ENSG00000169057.15735.30910539.890700.445602.669801.67110HepG2GNB2L1
MECP2ENSG00000169057.15721.60860549.682100.392502.574901.94860HepG2HNRNPA1
MECP2ENSG00000169057.15894.18740611.418400.548304.886903.98480HepG2HNRNPC
MECP2ENSG00000169057.15947.58310726.124600.383902.598401.91960HepG2HNRNPK
MECP2ENSG00000169057.15949.09580744.039300.351102.172801.60850HepG2HNRNPLL
MECP2ENSG00000169057.15356.83310674.34670-0.9182011.697309.98300HepG2HNRNPM
MECP2ENSG00000169057.151098.11290728.442700.592005.242204.33900HepG2IGF2BP2
MECP2ENSG00000169057.151041.12550732.804600.506503.074502.00930HepG2KHSRP
MECP2ENSG00000169057.15990.01600773.053500.356802.146301.56630HepG2MAGOH
MECP2ENSG00000169057.151121.96590795.593000.495803.669802.94450HepG2NCBP2
MECP2ENSG00000169057.151032.96690769.768300.424204.697803.70390HepG2NOL12
MECP2ENSG00000169057.15937.50990708.326500.404302.610601.90720HepG2PABPC4
MECP2ENSG00000169057.151071.87220777.153700.463803.833203.14340HepG2PCBP1
MECP2ENSG00000169057.151000.77560761.684500.393804.337203.39270HepG2PRPF4
MECP2ENSG00000169057.15930.24580780.102700.253802.721302.13220HepG2PUM1
MECP2ENSG00000169057.15619.49280779.51070-0.331502.205601.60420HepG2PUM2
MECP2ENSG00000169057.151274.87130777.263100.7138010.513709.25880HepG2RBM25
MECP2ENSG00000169057.151026.75950819.769300.324703.400702.72620HepG2RECQL
MECP2ENSG00000169057.15961.41540632.917600.603003.626602.54610HepG2RPS10
MECP2ENSG00000169057.15760.27590588.295500.369902.069901.37170HepG2RPS2
MECP2ENSG00000169057.151155.23580736.443800.649407.202806.11060HepG2SF3B1
MECP2ENSG00000169057.15929.15230704.672100.398905.261204.34490HepG2SLBP
MECP2ENSG00000169057.151550.145301240.573400.321303.235802.70750HepG2SRFBP1
MECP2ENSG00000169057.151039.59650725.592000.518707.782706.80320HepG2SRSF5
MECP2ENSG00000169057.151106.59900699.166300.6623012.9958011.95690HepG2SRSF9
MECP2ENSG00000169057.15820.458301077.09300-0.392604.812404.17260HepG2SSB
MECP2ENSG00000169057.151033.46040825.161400.324603.943203.22430HepG2SUCLG1
MECP2ENSG00000169057.15753.79790590.184300.352902.718502.22170HepG2TIAL1
MECP2ENSG00000169057.151122.01050717.967700.644008.524706.85150HepG2TUFM
MECP2ENSG00000169057.151198.25520836.110800.519104.466603.67790HepG2U2AF1
MECP2ENSG00000169057.15870.734901010.97390-0.215401.992801.50790HepG2ZNF622
MECP2ENSG00000169057.15882.95110643.725200.455802.543601.30600HepG2ZRANB2
MECP2ENSG00000169057.152471.731501945.557800.345205.468104.71310K562AGGF1
MECP2ENSG00000169057.152616.219202024.420100.369903.585802.83850K562AKAP8L
MECP2ENSG00000169057.153364.668902157.506300.641009.746608.74980K562AQR
MECP2ENSG00000169057.151822.563601399.668800.380803.120902.19270K562BOP1
MECP2ENSG00000169057.152831.923203423.15400-0.273502.805201.82440K562CELF1
MECP2ENSG00000169057.151316.570601050.157800.326102.175501.36550K562EFTUD2
MECP2ENSG00000169057.151281.480501017.029600.333303.370502.07210K562EIF4B
MECP2ENSG00000169057.153214.339102784.240200.207102.547601.64140K562EIF4G2
MECP2ENSG00000169057.151277.94620985.801200.374403.762502.22300K562EWSR1
MECP2ENSG00000169057.151605.545801174.478400.450906.569205.69380K562FXR1
MECP2ENSG00000169057.15457.65390636.53490-0.476002.574701.89750K562G3BP2
MECP2ENSG00000169057.152118.843102546.73400-0.265402.351201.65190K562GRSF1
MECP2ENSG00000169057.152330.771301481.176500.654002.889501.63970K562HNRNPA2B1
MECP2ENSG00000169057.151544.982801125.676800.456704.966704.18960K562HNRNPC
MECP2ENSG00000169057.15848.006701454.81520-0.778708.764906.94050K562HNRNPM
MECP2ENSG00000169057.151496.024601811.09010-0.275702.242601.43800K562HNRNPU
MECP2ENSG00000169057.151953.880202398.01240-0.295502.272501.54710K562HSPD1
MECP2ENSG00000169057.151597.208001916.66150-0.263003.257602.38260K562MTPAP
MECP2ENSG00000169057.151478.564401127.750500.390604.128502.61040K562NCBP2
MECP2ENSG00000169057.151864.997202129.52620-0.191402.583201.85880K562NSUN2
MECP2ENSG00000169057.151966.886101295.360100.602502.512401.58090K562PABPN1
MECP2ENSG00000169057.153359.959802007.819900.742705.426304.69860K562PUS1
MECP2ENSG00000169057.152975.658301956.193100.605104.906804.13790K562RBM15
MECP2ENSG00000169057.151297.821001001.636900.373602.667901.84340K562RBM22
MECP2ENSG00000169057.15784.66000603.365100.378902.772301.44210K562RBM25
MECP2ENSG00000169057.151968.727001184.495900.732902.976601.71300K562SF1
MECP2ENSG00000169057.15896.31580661.514000.438104.241903.17750K562SF3B4
MECP2ENSG00000169057.151776.283801024.591100.793707.424706.27690K562SRSF1
MECP2ENSG00000169057.151297.25870979.541300.405203.722702.47410K562SRSF4
MECP2ENSG00000169057.15703.06510871.86360-0.310402.595801.36250K562SSB
MECP2ENSG00000169057.151660.275201036.756600.679208.478707.15260K562TARDBP
MECP2ENSG00000169057.151364.811701000.120400.448403.830702.84510K562TIA1
MECP2ENSG00000169057.151315.48180981.880700.421902.728501.64560K562TRA2A
MECP2ENSG00000169057.151565.744102009.18970-0.359806.206004.99200K562WDR43
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for MECP2 is not available on ENCORE database.





23 RBPs can affect the splicing of MECP2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631200.254000.000000.00001includedK562SRSF1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.224000.000000.00002excludedK562SF3B4
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.207000.000850.03317excludedK562PRPF8
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.182000.000260.09985excludedK562SMNDC1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.322000.000000.00000excludedK562AKAP8L
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631480.392000.000000.00142includedHepG2ILF3
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.203000.000120.03980excludedK562WDR43
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631480.236000.000070.08972includedK562PHF6
SEMECP2ENSG00000169057.15chrX-1533052421533053721532978841532980081533576411533577650.085000.000000.00004includedK562AQR
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631200.255000.000000.00008includedK562FXR1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.254000.000030.00523excludedK562SUPV3L1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.338000.000000.00000excludedK562PUS1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.242000.000100.04187excludedK562UCHL5
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.228000.000110.03308excludedK562GPKOW
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.402000.000000.00000excludedK562EFTUD2
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631480.286000.000080.09404includedK562ILF3
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.198000.000770.09831excludedK562SRSF7
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.246000.000000.00000excludedK562UTP18
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.281000.000000.00005excludedK562HNRNPU
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.266000.000000.00000excludedK562RBM15
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363120-0.214000.000000.00000excludedK562AGGF1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.291000.000000.00009excludedK562SF3B1
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.472000.000040.00330excludedHepG2HNRNPU
SEMECP2ENSG00000169057.15chrX-153357641153357765153297657153298008153363060153363148-0.244000.000080.02655excludedK562SERBP1
SEMECP2ENSG00000169057.15chrX-1533576411533577651532976571532980081533630601533631200.263000.000000.00021includedK562PTBP1

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for MECP2 is not avaliable on ENCORE database.