RIOK2

General Information

Gene Name       : RIOK2
Gene ID             : ENSG00000058729.10
Essential Gene : Yes

Functions

Localization

Domains (AlphaFold: RIOK2)


Antibody Validation

 View validation images for all RIOK2 antibodies

Expression Level in Cell lines

Cell Line Gene Name Ensembl ID Rep1 TPM Rep2 TPM Average TPM Experiment ID
Gm12878RIOK2ENSG00000058729.1019.2315.6217.43ENCSR000AEF
HepG2RIOK2ENSG00000058729.1012.2919.6815.98ENCSR985KAT
K562RIOK2ENSG00000058729.1017.6615.3716.52ENCSR000AEO

Assays

2 Released experiments are available on ENCORE database.

Assay TypeExp_NameENCODE IDTarget labelBiosampleStatusLinked AntibodyControlsMethodNote
knockdown followed by RNA-seq RIOK2-BGKcLV30-K562ENCSR262ZBERIOK2K562ReleasedENCSR347NKX   CRISPRKD% = 50.0,61.0
knockdown followed by RNA-seq RIOK2-BGHcLV32-HepG2ENCSR485ZHWRIOK2HepG2ReleasedENCSR911CLD   CRISPRKD% = 87.0,75.7






  eCLIP  

No eCLIP experiment available



  Antibody Validation  
  GeneName    Antibody_DCC_ID    Product_ID    Lot_ID    Phosphate    HepG2 Primary Validation    K562 Primary Validation    HepG2 Secondary Validation    K562 Secondary Validation  
RIOK2ENCAB828CEYA302-449A1Released, ENCODE4BGHcLV32-33, Released, ENCODE4BGKcLV30-35, Released, ENCODE4
RIOK2ENCAB531RFUA302-450A1Released, ENCODE4BGHcLV32-33, Released, ENCODE4BGKcLV30-35, Released, ENCODE4


  KD RNA-Seq  
  GeneName    Cell_Line    Method    Exp_ID    Control_ID    Sample_reps    Control_reps    KD%    Status    Project    Note  
RIOK2K562CRISPRENCSR262ZBEENCSR347NKXRIOK2-BGKcLV30-35,RIOK2-BGKcLV30-36NT-BGKcLV30-1,NT-BGKcLV30-250.0,61.0ReleasedENCODE4
RIOK2HepG2CRISPRENCSR485ZHWENCSR911CLDRIOK2-BGHcLV32-33B,RIOK2-BGHcLV32-34BNT-BGHcLV32-1B,NT-BGHcLV32-2B87.0,75.7ReleasedENCODE4


  RNA Bind-n-Seqn  

No RBNS experiment available



  Localization Image in HepG2  

No Localization Information available













RBP Antibody InfoPrimary-HepG2Secondary-HepG2Primary-K562Secondary-K562Primary-UBERONPrimary-Hela
RIOK2Product_ID: A302-449A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RIOK2-human		 RIOK2-HEPG2-CRISPR-A302-449A.png<br>Caption: Western blot following CRISPR against RIOK2 in HepG2 whole cell lysate using RIOK2 specific antibody. Lane 1 is a ladder, lane 2 is HepG2 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RIOK2. RIOK2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
 Bethyl_A302-449A_1_RIOK2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RIOK2 specific antibody, A302-449A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RIOK2-specific antibody, A302-449A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RIOK2-K562-CRISPR-A302-449A.png<br>Caption: Western blot following CRISPR against RIOK2 in K562 whole cell lysate using RIOK2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RIOK2. RIOK2 protein appears as the green arrow, GAPDH serves as a control and appears in red arrow.
RIOK2Product_ID: A302-450A
Lot_ID: 1
Source: Bethyl Labs
Target Name: RIOK2-human		 Bethyl_A302-450A_1_RIOK2.png<br>Caption: IP-WB analysis of K562 whole cell lysate using the RIOK2 specific antibody, A302-450A. Lanes 1 and 2 are 2.5% of five million whole cell lysate input and 50% of IP enrichment, respectively, using a normal IgG antibody. Lane 3 is 50% of IP enrichment from five million whole cell lysate using the RIOK2-specific antibody, A302-450A. The same antibody was used to detect protein levels via Western blot. This antibody passes preliminary validation and will be further pursued for secondary validation. *NOTE* Protein sizes are taken from Genecards.org and are only estimates based on sequence. Actual protein size may differ based on protein characteristics and electrophoresis method used.
 RIOK2-K562-CRISPR-A302-450A.png<br>Caption: Western blot following CRISPR against RIOK2 in K562 whole cell lysate using RIOK2 specific antibody. Lane 1 is a ladder, lane 2 is K562 non-targeting control knockdown, lane 3 and 4 are two different CRISPR against RIOK2. RIOK2 protein appears as the green arrow, Beta-actin serves as a control and appears in red arrow.








RNA Targets

  • eCLIP assay for RIOK2 is not avaliable on ENCORE database.



Proteins bound to RNA
  • RIOK2 RNA is bound by 1 proteins.  







eCLIP assay for RIOK2 is not avaliable on ENCORE database.





1 proteins are bound to RIOK2 RNA:

Chr Start End Strand Matched Area log2(fold change) -log(p-value) Cell Line Binding Protein Name
chr59651882996518860-CDS,intron-Distal4.591694.54356K562DDX3X
ChrStartEndStrandMatched Arealog2(fold change)-log(p-value)Cell LineBinding Protein Name




eCLIP assay for RIOK2 is not avaliable on ENCORE database.



Diffrential Expression

  • The Knockdown/knockout assay for RIOK2 is not available on ENCORE database.

  • 61 RBPs can affect the levels of RIOK2.  








The Knockdown/knockout RNA-Seq assay for RIOK2 is not available on ENCORE database.





61 RBPs can affect the gene expression of RIOK2 :

Gene NameGene IDKDMeanCNMeanlog2(fold change) *-log(pval) **-log(padj) **Cell LineAffected by RBPBound by RBP
RIOK2ENSG00000058729.6490.92830397.390300.304902.053401.51480HepG2ACO1
RIOK2ENSG00000058729.6768.54240584.452000.394901.958201.50180HepG2AKAP1
RIOK2ENSG00000058729.6640.69240461.977200.471703.864702.97560HepG2CALR
RIOK2ENSG00000058729.6492.68000397.294700.310302.153501.55610HepG2CKAP4
RIOK2ENSG00000058729.6347.65870479.35090-0.463402.657501.98220HepG2CPSF6
RIOK2ENSG00000058729.6488.77090338.531000.529803.537102.77780HepG2DDX21
RIOK2ENSG00000058729.6290.12770544.33750-0.907805.190303.86390HepG2DDX59
RIOK2ENSG00000058729.6351.54210480.87580-0.452002.140701.59060HepG2DDX6
RIOK2ENSG00000058729.6378.58450269.942400.487902.547301.48110HepG2EFTUD2
RIOK2ENSG00000058729.6312.41130462.36540-0.565603.300702.65700HepG2EIF2S1
RIOK2ENSG00000058729.6759.42730582.348500.382903.472802.55080HepG2EIF4G1
RIOK2ENSG00000058729.6647.33880448.252500.530103.113602.30210HepG2FIP1L1
RIOK2ENSG00000058729.6295.99280478.23210-0.692104.671903.81810HepG2G3BP1
RIOK2ENSG00000058729.6335.27970543.38690-0.696604.812903.98340HepG2GPKOW
RIOK2ENSG00000058729.6387.25640584.17680-0.593102.886302.15530HepG2HNRNPF
RIOK2ENSG00000058729.6359.24670575.25880-0.679203.957203.10490HepG2IGF2BP1
RIOK2ENSG00000058729.6753.06210518.546000.538203.508102.80530HepG2MATR3
RIOK2ENSG00000058729.6594.18700424.552500.484904.656303.90320HepG2PCBP1
RIOK2ENSG00000058729.6645.58300432.015000.579406.622205.84780HepG2PTBP1
RIOK2ENSG00000058729.6720.54010593.130700.280602.407801.84710HepG2PUM1
RIOK2ENSG00000058729.6583.39750404.766400.527305.571804.75320HepG2PUS1
RIOK2ENSG00000058729.6783.23860630.427300.313002.389801.89900HepG2RBM22
RIOK2ENSG00000058729.6220.25050326.97940-0.570103.018302.35110HepG2SAFB2
RIOK2ENSG00000058729.6749.73020447.432000.744605.708404.71850HepG2SF3A3
RIOK2ENSG00000058729.6608.53410449.752900.436103.756803.08380HepG2SF3B4
RIOK2ENSG00000058729.6906.63860712.051800.348403.731903.17680HepG2SRFBP1
RIOK2ENSG00000058729.61044.81950469.457101.1541021.6504020.59400HepG2SRSF1
RIOK2ENSG00000058729.6403.50720552.28080-0.452802.267701.37120HepG2SRSF3
RIOK2ENSG00000058729.6373.84820573.89500-0.618303.590202.81280HepG2SRSF7
RIOK2ENSG00000058729.6758.25910618.242400.294402.531902.03600HepG2SSB
RIOK2ENSG00000058729.6245.63270422.00090-0.780707.188606.35760HepG2TAF15
RIOK2ENSG00000058729.6381.08910505.42480-0.407402.223701.56380HepG2TARDBP
RIOK2ENSG00000058729.6779.20270383.527601.0226012.6357011.26050HepG2U2AF1
RIOK2ENSG00000058729.6553.25640383.099000.530104.958804.19680HepG2UTP3
RIOK2ENSG00000058729.6608.00090404.841400.586603.234202.08550HepG2XRCC6
RIOK2ENSG00000058729.6216.04910317.92130-0.557303.811203.04470HepG2ZC3H8
RIOK2ENSG00000058729.6797.53890480.788100.730105.897604.10790K562AARS
RIOK2ENSG00000058729.61746.195901319.994900.403603.378902.74380K562AQR
RIOK2ENSG00000058729.6150.65110245.24430-0.703003.791002.51680K562CCAR1
RIOK2ENSG00000058729.6757.788301071.33690-0.499502.331701.37180K562CNOT7
RIOK2ENSG00000058729.6484.46650652.54100-0.429702.930002.12360K562CPSF6
RIOK2ENSG00000058729.61913.972301099.413600.7997010.993809.79260K562DDX47
RIOK2ENSG00000058729.61160.97520776.620700.580008.609606.90400K562DKC1
RIOK2ENSG00000058729.6742.87970995.90930-0.422903.718802.90680K562EIF2S2
RIOK2ENSG00000058729.6812.74190605.466000.424702.237501.49680K562EIF4A3
RIOK2ENSG00000058729.6901.14590629.660300.517106.251605.39650K562FXR1
RIOK2ENSG00000058729.61078.52950660.756400.706805.687104.59120K562G3BP2
RIOK2ENSG00000058729.6328.01960526.36910-0.682303.047502.42430K562HNRNPC
RIOK2ENSG00000058729.6966.72000765.910300.335802.192301.46820K562KIF1C
RIOK2ENSG00000058729.61062.26990858.010600.308002.379301.54490K562MBNL1
RIOK2ENSG00000058729.6809.06800601.401700.427802.214601.44640K562NAA15
RIOK2ENSG00000058729.6617.94750784.62510-0.344503.648302.80400K562NSUN2
RIOK2ENSG00000058729.61066.20450800.724200.413003.364802.28690K562PCBP2
RIOK2ENSG00000058729.6561.61150449.368900.321602.012701.32880K562POLR2G
RIOK2ENSG00000058729.61145.93560549.366501.0606015.8236014.00990K562PRPF8
RIOK2ENSG00000058729.62150.411501597.956500.428303.292502.40290K562PUF60
RIOK2ENSG00000058729.61224.05730715.753500.774006.041205.27640K562PUS1
RIOK2ENSG00000058729.6943.30840559.643600.753107.197705.86490K562RPS19
RIOK2ENSG00000058729.6920.97080687.033700.422704.081203.03600K562SF3B4
RIOK2ENSG00000058729.6255.31310408.01100-0.676303.739002.49590K562SRFBP1
RIOK2ENSG00000058729.61176.70390857.074700.457204.676503.76190K562SRSF1
RIOK2ENSG00000058729.6292.98710405.70740-0.469602.554701.55030K562SRSF4
RIOK2ENSG00000058729.6809.62210450.728000.8449011.8687010.16870K562SYNCRIP
RIOK2ENSG00000058729.6650.33400867.46530-0.415602.756302.04990K562TARDBP
RIOK2ENSG00000058729.6281.68500453.91880-0.688403.950702.81850K562TIAL1
RIOK2ENSG00000058729.6310.63420482.03860-0.633903.464302.38470K562TRIM56
RIOK2ENSG00000058729.61131.80990640.446400.821408.853906.72180K562U2AF1
RIOK2ENSG00000058729.6901.00950661.455200.445804.301803.30470K562U2AF2
RIOK2ENSG00000058729.61077.685401487.19770-0.464703.765502.73000K562ZC3H8
Gene NameGene IDKDMeanCNMeanlog2(fold change)-log(pval)-log(padj)Cell LineAffected by RBPBound by RBP

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".






The Knockdown/knockout RNA-Seq assay for RIOK2 is not available on ENCORE database.





2 RBPs can affect the splicing of RIOK2 :

Event TypeGene NameGene IDchrStrandExonStartExonEnd2ndExonStart2ndExonEndupstreamESupstreamEEdownstreamESdownstreamEEdPSIp-valueFDRis_includedCell LineAffected by RBPBound by RBP
SERIOK2ENSG00000058729.6chr5-965088789650899396506909965071019651281996513067-0.098000.000100.00173excludedK562AQR
MXERIOK2ENSG00000058729.6chr5-96513435965134859651475896514946965128199651299596518829965189640.051000.000150.001771includedK562AQR
MXERIOK2ENSG00000058729.6chr5-9651343596513552965147589651489796512819965129959651882996518964-0.175000.000140.003202includedK562U2AF2

*: "Inf" was shown as "99999.99999" and "-Inf" was shown as "-99999.99999".
**: "-log(0)" was displayed as "99999.99999".





The Knockdown/knockout RNA-Seq assay for RIOK2 is not avaliable on ENCORE database.